Enzyme:substrate hydrogen bond shortening during the acylation phase of serine protease catalysis.

abstract：:The three-dimensional solution structure of the repeating -PO4-6Gal beta 1-4Man alpha 1- disaccharide fragment of the lipophosphoglycan (LPG) derived from Leishmania donovani has been determined by use of a combination of homo- and heteronuclear NMR spin coupling constant measurements together with restrained molecula...

journal_title：Biochemistry

authors： Homans SW,Mehlert A,Turco SJ

更新日期：1992-01-28 00:00:00

abstract：:Stopped-flow experiments obtained in the pre-steady-state time scale of the NAD-malic enzyme reaction exhibit a lag prior to the attainment of steady state. Previous results from isotope effect studies in which the deuterium isotope effect on Vmax decreases to a value of 1 at low pH have been interpreted as suggesting...

journal_title：Biochemistry

authors： Rajapaksa R,Abu-Soud H,Raushel FM,Harris BG,Cook PF

更新日期：1993-03-02 00:00:00

abstract：:Pancreatic phospholipase A(2) (PLA2) shows a strong preference for the binding to the anionic interface and a consequent allosteric activation. In this paper, we show that virtually all the preference is mediated through 3 (Lys-53, -56, and -120) of the 12 cationic residues of bovine pancreatic PLA2. The lysine-to-met...

journal_title：Biochemistry

authors： Yu BZ,Poi MJ,Ramagopal UA,Jain R,Ramakumar S,Berg OG,Tsai MD,Sekar K,Jain MK

更新日期：2000-10-10 00:00:00

abstract：:Transcarboxylase is made up of a central hexameric subunit (S20,W similar 12 S), three peripheral dimeric metallo subunits (S20,W similar to 5 S), and six biotinyl carboxyl carrier subunits (S20,W similar to 1.3 S). The results presented here show that the carboxyl carrier subunit is required for assembly of the 12S a...

journal_title：Biochemistry

authors： Ahmad F,Jacobson B,Chuang M,Brattin W,Wood HG

更新日期：1975-04-22 00:00:00

abstract：:Calmodulin (CaM) is a ubiquitous protein in nature and plays a regulatory role in numerous biological processes, including the upregulation of nitric oxide (NO) synthesis in vivo. Several peptides that prevent NO production by interacting with CaM have been isolated in the cutaneous secretions of Australian amphibians...

journal_title：Biochemistry

authors： Calabrese AN,Bowie JH,Pukala TL

更新日期：2015-01-20 00:00:00

abstract：:The fluorescence spectrum of C1 inhibitor (C1-Inh) in aqueous buffer has a maximum at 324 nm which shifts to 358 nm in 6.0 M guanidinium chloride (GdmC1), indicating that fluorescent tryptophans are buried in the native protein. When titrated with GdmC1, the fluorescence intensity, polarization, and emission maximum o...

journal_title：Biochemistry

authors： Lennick M,Brew SA,Ingham KC

更新日期：1985-05-07 00:00:00

abstract：:The partition of cis-parinaric acid (9,11,13,15-cis, trans, trans,cis-octadecatetraenoic acid, cis-PnA) and trans-parinaric acid (9,11,13,15-all-trans-octadecatetraenoic acid, trans-PnA) among aqueous, solid lipid, and fluid lipid phases has been measured by three spectroscopic parameters: absorption spectral shifts, ...

journal_title：Biochemistry

authors： Sklar LA,Miljanich GP,Dratz EA

更新日期：1979-05-01 00:00:00

abstract：:The Fpg protein from Escherichia coli is a multifunctional protein that excises damaged purine bases from DNA to generate aldehydic abasic sites and then catalyzes the successive cleavage of the phosphodiester bonds first on the 3'-side and then on the 5'-side of the abasic site to generate 5'- and 3'-phosphate ends, ...

journal_title：Biochemistry

authors： Bhagwat M,Gerlt JA

更新日期：1996-01-16 00:00:00

abstract：:DNA sequences with repetitive G-rich structural motifs, which form special structures called G-quadruplexes, widely exist in the human genome. Here we report the general peroxidase activity of G-quadruplex-hemin complexes and discuss the connection between peroxidase activity and G-quadruplex structures. The high pero...

journal_title：Biochemistry

authors： Cheng X,Liu X,Bing T,Cao Z,Shangguan D

更新日期：2009-08-25 00:00:00

abstract：:In cytochrome c, the coordination of the axial Met Sδ atom to the heme Fe atom occurs in one of two distinctly different stereochemical manners, i.e., R and S configurations, depending upon which of the two lone pairs of the Sδ atom is involved in the bond; hence, the Fe-coordinated Sδ atom becomes a chiral center. In...

journal_title：Biochemistry

authors： Tai H,Tonegawa K,Shibata T,Hemmi H,Kobayashi N,Yamamoto Y

更新日期：2013-07-16 00:00:00

abstract：:Several furanocoumarins were tested for their ability to inhibit human P450 2A6 activity. The metabolites and conjugates formed from these furanocoumarins after incubation with reconstituted purified P450 2A6 in the absence and presence of exogenous nucleophiles were characterized by UV and LC/ESI-MS/MS analysis. The ...

journal_title：Biochemistry

authors： Koenigs LL,Trager WF

更新日期：1998-07-14 00:00:00

abstract：:The doublet at 850 and 830 cm-1 in the Raman spectra of proteins containing tyrosyl residues has been examined as to its origin and the relation of its components to the environment of the phenyl ring, the state of the phenolic hydroxyl group, and the conformation of the amino acid backbone. Raman spectral studies on ...

journal_title：Biochemistry

authors： Siamwiza MN,Lord RC,Chen MC,Takamatsu T,Harada I,Matsuura H,Shimanouchi T

更新日期：1975-11-04 00:00:00

abstract：:We have examined the effects on redox kinetics of changing the reduction potential of the mu-oxo-bridged binuclear iron center in octameric hemerythrin (Hr) from Phascolopsis gouldii. The opportunity to examine such effects is provided by the availability of mu-sulfidomethemerythrin (mu-S2-metHr), whose [Fe(III),Fe(II...

journal_title：Biochemistry

authors： Pearce LL,Utecht RE,Kurtz DM Jr

更新日期：1987-12-29 00:00:00

abstract：:A minor fraction of plasma high-density lipoprotein (pre beta-1 HDL) has been shown to promote cholesterol efflux from peripheral cell membranes [Castro, G. R., & Fielding, C. J. (1988) Biochemistry 27, 25-29]. When isolated native plasma is incubated at 37 degrees C, this fraction is specifically decreased. On the ot...

journal_title：Biochemistry

authors： Miida T,Kawano M,Fielding CJ,Fielding PE

更新日期：1992-11-17 00:00:00

abstract：:DrrA and DrrB proteins form an ATP-dependent efflux pump for doxorubicin and daunorubicin in Streptomyces peucetius. DrrA, the catalytic subunit, forms a complex with the integral membrane protein DrrB. Previous studies have provided evidence for strong interaction between these two proteins, which was found to be cri...

journal_title：Biochemistry

authors： Rao DK,Kaur P

更新日期：2008-03-04 00:00:00

abstract：:Inosine has the ability to act as a "wild-card" binding nonspecifically to both A.T and G.C base pairs. This has obvious implications for the design of oligonucleotide site-directed probes. In this paper we present a series of oligonucleotides with a 5'pur9-pyr9-pyr9 motif which are designed to fold up sequentially in...

journal_title：Biochemistry

authors： Mills M,Völker J,Klump HH

更新日期：1996-10-15 00:00:00

abstract：:Previous optical and electron paramagnetic resonance (EPR) spectroscopic studies of the newly discovered peroxynitrite scavenging pseudoperoxidase from Leishmania major (LmPP) suggested that ferric LmPP contained a six-coordinate low-spin (6cLS) heme with a thiolate ligand, presumably a cysteine, bound to its heme iro...

journal_title：Biochemistry

authors： Saha R,Bose M,Sen Santara S,Roy J,Adak S

更新日期：2013-12-10 00:00:00

abstract：:The crystal structures of the guanosine 5'-monophosphate (GMP) and inosine 5'-monophosphate (IMP) complexes of Toxoplasma gondii hypoxanthine-guanine phosphoribosyltransferase (HGPRT) have been determined at 1.65 and 1.90 A resolution. These complexes, which crystallize in space groups P2(1) (a = 65.45 A, b = 90.84 A,...

journal_title：Biochemistry

authors： Héroux A,White EL,Ross LJ,Borhani DW

更新日期：1999-11-02 00:00:00

abstract：:If cytochrome c oxidase is subjected to pressure during the aerobic steady state, large spectral changes are apparent. These seem to be associated with the inhibition of electron transport within the oxidase. The volume change for the transition is about 80 mL/mol. When the oxidase in the aerobic steady state, with po...

journal_title：Biochemistry

authors： Kornblatt JA,Hui Bon Hoa G,Heremans K

更新日期：1988-07-12 00:00:00

abstract：:A novel liposomal method permits studies of Ca movements across the bilayers of multilamellar vesicles (MLV) which had entrapped the Ca-dependent, fluorescent indicator dye Fura 2. Ionomycin-mediated Ca translocation across MLV of phosphatidylcholine (PC)/dicetyl phosphate (DCP), 9:1, obeyed simple first-order kinetic...

journal_title：Biochemistry

authors： Blau L,Weissmann G

更新日期：1988-07-26 00:00:00

abstract：:The high resolution proton nuclear magnetic resonance (NMR) spectra of the synthetic DNA block polymer d(C15A15)-d(T15G15) were studied in order to more completely understand telestability in DNA, and to provide fundamental NMR data on DNA helices and random coils. Spectra were measured in the spectral region from 0 t...

journal_title：Biochemistry

authors： Early TA,Kearns DR,Burd JF,Larson JE,Wells RD

更新日期：1977-02-08 00:00:00

abstract：:We report here that the DNA strand scission by dynemicin A is not only sequence-specific but also conformation-specific. The salt-induced B----Z conformational transition dramatically enhanced the cleavage by dynemicin A in a B-Z junction region. By contrast, the bleomycin-Fe(II) complex, the elsamicin A-Fe(II) comple...

journal_title：Biochemistry

authors： Ichikawa A,Kuboya T,Aoyama T,Sugiura Y

更新日期：1992-07-28 00:00:00

abstract：:Because of its ability to selectively glucosylate misfolded glycoproteins, UDP-glucose:glycoprotein glucosyltransferase (UGGT) functions as a folding sensor in the glycoprotein quality control system in the endoplasmic reticulum (ER). The unique property of UGGT derives from its ability to transfer a glucose residue t...

journal_title：Biochemistry

authors： Ohara K,Takeda Y,Daikoku S,Hachisu M,Seko A,Ito Y

更新日期：2015-08-11 00:00:00

abstract：:Membrane fusion is involved in many fundamental cellular processes and entry of enveloped viruses into host cells. Influenza type A virus HA has long served as a paradigm for mechanistic studies of protein-mediated membrane fusion via large-scale structural rearrangements induced by acidic pH. Here we report the newly...

journal_title：Biochemistry

authors： Ni F,Chen X,Shen J,Wang Q

更新日期：2014-02-11 00:00:00

abstract：:The binding of agonists, antagonists, and the acetylcholinesterase inhibitor, eserine, to the nicotinic acetylcholine receptor from Torpedo californica has been monitored by the fluorescence changes of two extrinsic probes that have been covalently attached to the receptor protein. Although both probes, 5-(iodoacetami...

journal_title：Biochemistry

authors： Dunn SM,Raftery MA

更新日期：1993-08-24 00:00:00

abstract：:Ca2+-activated Z-disk-removing activity in the P0-40 crude muscle extracts described by Busch et al. (Busch, W. A., Stromer, M. H., Goll, D. E., and Suzuki, A. (1972), J. Cell Biol. 52, 367) was purified from porcine skeletal muscle extracts by using five column chromatographic procedures in succession: (1) 6% agarose...

journal_title：Biochemistry

authors： Dayton WR,Goll DE,Zeece MG,Robson RM,Reville WJ

更新日期：1976-05-18 00:00:00

abstract：:The conformational analysis of non-native states of proteins remains one of the most difficult problems in structural biology, because such states are represented by a superimposition of several states that are rapidly interconverting. Hence, model building of the conformational ensemble remains challenging, although ...

journal_title：Biochemistry

authors： Sziegat F,Silvers R,Hähnke M,Jensen MR,Blackledge M,Wirmer-Bartoschek J,Schwalbe H

更新日期：2012-04-24 00:00:00

abstract：:To determine the energetic and structural consequences of placing a charged group within the core of a protein, two "buried charge" mutants, Met 102----Lys (M102K) and Leu 133----Asp (L133D) were constructed in phage T4 lysozyme. Both proteins fold at neutral pH, although they are substantially less stable than wild t...

journal_title：Biochemistry

authors： Dao-pin S,Anderson DE,Baase WA,Dahlquist FW,Matthews BW

更新日期：1991-12-10 00:00:00

abstract：:Membranes derived from free floating granulosa cells in porcine ovarian follicular fluid were used as a starting material for structural characterization of both LH/hCG and FSH receptors. The receptors were highly hormone-specific and showed single classes of high-affinity binding sites (Kd = 19-74 pM). Their molecula...

journal_title：Biochemistry

authors： Yarney TA,Sairam MR,Bhargavi GN,Mohapatra SK

更新日期：1990-04-17 00:00:00

abstract：:SHP-1, a protein tyrosine phosphatase containing two tandem SH2 domains, is autoinhibited at rest by its N-terminal SH2 (N-SH2) domain. Relief from autoinhibition and a subsequent 10-60-fold increase in V(max) have been observed upon N-SH2 domain engagement by a specific phosphotyrosyl ligand or upon deletion of the S...

journal_title：Biochemistry

authors： Wang J,Walsh CT

更新日期：1997-03-11 00:00:00