Activation of DNA cleavage by dynemicin A in a B-Z conformational junction.

abstract：:Multielectron multiproton reactions play an important role in both biological systems and chemical reactions involved in energy storage and manipulation. A key strategy employed by nature in achieving such complex chemistry is the use of proton-coupled redox steps. Cytochrome c nitrite reductase (ccNiR) catalyzes the ...

journal_title：Biochemistry

authors： Judd ET,Stein N,Pacheco AA,Elliott SJ

更新日期：2014-09-09 00:00:00

abstract：:The dissociation of Semliki Forest virus induced by increasing concentrations of the anionic detergent sodium dodecyl sulfate was studied using density gradient centrifugation. Detectable binding to the virus started well below the critical micellar concentration of the detergent and increased thereafter with increase...

journal_title：Biochemistry

authors： Becker R,Helenius A,Simons K

更新日期：1975-05-06 00:00:00

abstract：:To maintain daily cycles, the circadian clock must tightly regulate the rhythms of thousands of mRNAs and proteins with the correct period, phase, and amplitude to ultimately drive the wide range of rhythmic biological processes. Recent genomic approaches have revolutionized our view of circadian gene expression and h...

journal_title：Biochemistry

authors： Kojima S,Green CB

更新日期：2015-01-20 00:00:00

abstract：:Information on the secondary structure and interactions of RNA is important to understand the biological function of RNA as well as in applying RNA as a tool for therapeutic purposes. Recently, the isoenergetic microarray mapping method was developed to improve the prediction of RNA secondary structure. Herein, for th...

journal_title：Biochemistry

authors： Fratczak A,Kierzek R,Kierzek E

更新日期：2011-09-06 00:00:00

abstract：:The physicochemical and catalytic properties of thioredoxin-T' are described. This complemented protein structure consists of a 1:1 complex between the inactive fragments thioredoxin-T-(1--73) and thioredoxin T-(74--108). These are generated by selective trypsin cleavage at Arg-73 in lysine-modified and denatured Esch...

journal_title：Biochemistry

authors： Slabý I,Holmgren A

更新日期：1979-12-11 00:00:00

abstract：:Deposition of amyloid beta-peptide (Abeta) into amyloid plaques is one of the invariant neuropathological features of Alzheimer's disease. Proteins that codeposit with Abeta are potentially important for the pathogenesis, and a recently discovered plaque-associated protein is the collagenous Alzheimer amyloid plaque c...

journal_title：Biochemistry

authors： Kakuyama H,Söderberg L,Horigome K,Winblad B,Dahlqvist C,Näslund J,Tjernberg LO

更新日期：2005-11-29 00:00:00

abstract：:Neuropeptide Y (NPY) and the pancreatic polypeptide (PP) are members of the neuropeptide Y family of hormones. They bind to the Y receptors with very different affinities: Whereas PP is highly selective for the Y(4) receptor, NPY displays highest affinites for Y(1), Y(2), and Y(5) receptor subtypes. Introducing the NP...

journal_title：Biochemistry

authors： Lerch M,Kamimori H,Folkers G,Aguilar MI,Beck-Sickinger AG,Zerbe O

更新日期：2005-06-28 00:00:00

abstract：:DNA polymerase (pol) β is a small eukaryotic DNA polymerase composed of two domains. Each domain contributes an enzymatic activity (DNA synthesis and deoxyribose phosphate lyase) during the repair of simple base lesions. These domains are termed the polymerase and lyase domains, respectively. Pol β has been an excelle...

journal_title：Biochemistry

authors： Beard WA,Wilson SH

更新日期：2014-05-06 00:00:00

abstract：:NSC 613862 (S)-(-) and NSC 613863 (R)-(+) are the two chiral isomers of ethyl-5-amino-2-methyl-1,2-dihydro-3-phenylpyrido[3, 4-b]pyrazin-7-yl carbamate. Both compounds bind to tubulin in a region that overlaps the colchicine site. They induce formation of abnormal polymers from purified GTP-Mg-tubulin, the active asse...

journal_title：Biochemistry

authors： Barbier P,Peyrot V,Leynadier D,Andreu JM

更新日期：1998-01-13 00:00:00

abstract：:The salivary antimicrobial peptide histatin-5 is able to aggregate and fuse negatively charged small unilamellar vesicles, and this fusogenic activity is selectively induced by the presence of zinc ions. Circular dichroism spectroscopy shows that histatin-5, in the presence of negatively charged vesicles and zinc ions...

journal_title：Biochemistry

authors： Melino S,Rufini S,Sette M,Morero R,Grottesi A,Paci M,Petruzzelli R

更新日期：1999-07-27 00:00:00

abstract：:Phospholipase B1 (PLB1), secreted by the pathogenic yeast Cryptococcus neoformans, has an established role in virulence. Although the mechanism of its phospholipase B, lysophospholipase, and lysophospholipase transacylase activities is unknown, it possesses lipase, subtilisin protease aspartate, and phospholipase moti...

journal_title：Biochemistry

authors： Jones PM,Turner KM,Djordjevic JT,Sorrell TC,Wright LC,George AM

更新日期：2007-09-04 00:00:00

abstract：:Eosinophil cationic protein (ECP) is a component of the eosinophil granule matrix. It shows marked toxicity against helminth parasites, bacteria single-stranded RNA viruses, and host epithelial cells. Secretion of human ECP is related to eosinophil-associated allergic, asthmatic, and inflammatory diseases. ECP belongs...

journal_title：Biochemistry

authors： Mohan CG,Boix E,Evans HR,Nikolovski Z,Nogués MV,Cuchillo CM,Acharya KR

更新日期：2002-10-08 00:00:00

abstract：:Fesselin is a proline-rich actin-binding protein that was isolated from avian smooth muscle. Fesselin bundles actin and accelerates actin polymerization by facilitating nucleation. We now show that this polymerization of actin can be regulated by Ca(2+)-calmodulin. Fesselin was shown to bind to immobilized calmodulin ...

journal_title：Biochemistry

authors： Schroeter M,Chalovich JM

更新日期：2004-11-02 00:00:00

abstract：:Pulmonary surfactant forms a monolayer of lipids and proteins at the alveolar air/liquid interface. Although cholesterol is a natural component of surfactant, its function in surface dynamics is unclear. To further elucidate the role of cholesterol in surfactant, we used a captive bubble surfactometer (CBS) to measure...

journal_title：Biochemistry

authors： Diemel RV,Snel MM,Van Golde LM,Putz G,Haagsman HP,Batenburg JJ

更新日期：2002-12-17 00:00:00

abstract：:We have measured the rates and efficiencies of DNA unwinding (the number of ATP molecules hydrolyzed per DNA base pair unwound) catalyzed by the RecBC,RecBCD-K177Q (a site-directed mutant in the putative ATP-binding site in the RecD subunit), and RecBCD enzymes from Escherichia coli. The DNA unwinding rate was measure...

journal_title：Biochemistry

authors： Korangy F,Julin DA

更新日期：1994-08-16 00:00:00

abstract：:We report two small molecule azobenzenesulfonamide probes, CAP1 and CAP2, capable of photomodulating the activity of carbonic anhydrase (CA) on demand. In the trans form, CAP azobenzene probes adopt a linear shape, making them suitable for occupying the CA active site and interacting with Zn2+, thereby inhibiting enzy...

journal_title：Biochemistry

authors： Aggarwal K,Banik M,Medellin B,Que EL

更新日期：2019-01-08 00:00:00

abstract：:Protein electrochemistry in an ultra-thin-layer electrochemical cell suitable for UV/vis and IR spectroscopy has been used to characterize the vibrational modes of the primary electron donors of Rhodobacter sphaeroides and Rhodopseudomonas viridis reaction centers in their neutral and cation radical states (P and P+, ...

journal_title：Biochemistry

authors： Leonhard M,Mäntele W

更新日期：1993-05-04 00:00:00

abstract：:The fluorescence intensity of the two flavin prosthetic groups, FMN and FAD, in neuronal nitric oxide synthase (nNOS) was found to decay highly nonexponentially, being best described by four fluorescence lifetimes. This excited state heterogeneity is the result of multiple flavin quenching sites which are due to sever...

journal_title：Biochemistry

authors： Brunner K,Tortschanoff A,Hemmens B,Andrew PJ,Mayer B,Kungl AJ

更新日期：1998-12-15 00:00:00

abstract：:Previous optical and electron paramagnetic resonance (EPR) spectroscopic studies of the newly discovered peroxynitrite scavenging pseudoperoxidase from Leishmania major (LmPP) suggested that ferric LmPP contained a six-coordinate low-spin (6cLS) heme with a thiolate ligand, presumably a cysteine, bound to its heme iro...

journal_title：Biochemistry

authors： Saha R,Bose M,Sen Santara S,Roy J,Adak S

更新日期：2013-12-10 00:00:00

abstract：:The formation of pseudouridine (psi) on U5 small nuclear RNA (U5 snRNA) was studied using an in vitro modification system. Labeled U5 RNA, synthesized in vitro and therefore unmodified, was incubated in reactions containing S100 and/or nuclear extracts (NE) from HeLa cells, and the levels of psi were determined. There...

journal_title：Biochemistry

authors： Patton JR

更新日期：1994-08-30 00:00:00

abstract：:The folding of the enzyme indole-3-glycerol-phosphate synthase (IGPS), a member of the (alpha/beta)8 fold family, has been studied. At least two folding intermediates have been detected using spectroscopic and activity measurements in combination with gel filtration chromatography. These two intermediates are produced...

journal_title：Biochemistry

authors： Sánchez del Pino MM,Fersht AR

更新日期：1997-05-06 00:00:00

abstract：:A galactoside-binding lectin (hL-31) containing a collagen-like sequence was identified in human tumor cells. It was found to be the homologue of the IgE-binding protein, the macrophage cell-surface Mac-2 antigen, and the murine CBP35, RL-29, and mL-34 lectins. Here we report on the expression in Escherichia coli and ...

journal_title：Biochemistry

authors： Ochieng J,Platt D,Tait L,Hogan V,Raz T,Carmi P,Raz A

更新日期：1993-04-27 00:00:00

abstract：:Discoidal high-density lipoprotein (HDL) particles are known to be fractionalized into several discrete populations in plasma and to differ in behavior according to size; however, their structural differences and the factors regulating their size are less understood. In this study, we prepared several reconstituted HD...

journal_title：Biochemistry

authors： Miyazaki M,Nakano M,Fukuda M,Handa T

更新日期：2009-08-18 00:00:00

abstract：:Human cystathionine beta-synthase (CBS) is an essential enzyme for the removal of the toxic metabolite homocysteine. Heme and pyridoxal phosphate (PLP) cofactors are necessary to catalyze the condensation of homocysteine and serine to generate cystathionine. While the role for the PLP cofactor is thought to be similar...

journal_title：Biochemistry

authors： Green EL,Taoka S,Banerjee R,Loehr TM

更新日期：2001-01-16 00:00:00

abstract：:Phe-tRNAPhe species modified on the 3'-terminal ribose residue were investigated for their ability to participate in individual steps of the elongation cycle using eukaryotic ribosomes from reticulocytes. None of the Phe-tRNAs used, namely Phe-tRNAPhe-C-C-3'dA, Phe-tRNAPhe-C-C-3'-NH2A, and Phe-tRNAPhe-C-C-Aoxi-red, ca...

journal_title：Biochemistry

authors： Baksht E,de Groot N,Sprinzl M,Cramer F

更新日期：1976-08-10 00:00:00

abstract：:Pluronic block copolymers (triblock copolymers of poly(ethylene oxide) and poly(propylene oxide)) exhibit a chemosensitizing effect on multidrug resistant cell lines. Changes in membrane permeability are hypothesized to be responsible because inhibition of drug transport mediated by both the multidrug-resistance-assoc...

journal_title：Biochemistry

authors： Krylova OO,Pohl P

更新日期：2004-03-30 00:00:00

abstract：:Self-splicing of Tetrahymena pre-rRNA proceeds in two consecutive phosphoryl transesterification steps. One major difference between these steps is that in the first an exogenous guanosine (G) binds to the active site, while in the second the 3'-terminal G414 residue of the intron binds. The first step has been extens...

journal_title：Biochemistry

authors： Mei R,Herschlag D

更新日期：1996-05-07 00:00:00

abstract：:The crystal structure of the heme-copper oxidases suggested a putative channel of oxygen entry into the heme-copper site of O(2) reduction. Changing a conserved valine near this center in cytochrome bo(3) of Escherichia coli to isoleucine caused a significant increase in the apparent K(M) for oxygen with little or no ...

journal_title：Biochemistry

authors： Riistama S,Puustinen A,Verkhovsky MI,Morgan JE,Wikström M

更新日期：2000-05-30 00:00:00

abstract：:Lipoprotein(a) [Lp(a)] is a unique lipoprotein consisting of a low-density lipoprotein moiety (LDL) covalently linked to apoprotein(a). Previous work has demonstrated that Lp(a) can compete with plasminogen (PGN) for binding to endothelial and mononuclear cells and can inhibit PGN activation in cell-free systems. We h...

journal_title：Biochemistry

authors： Ezratty A,Simon DI,Loscalzo J

更新日期：1993-05-04 00:00:00

abstract：:Diphtheria toxin (DT) has been studied as a model for understanding active-site structure and function in the ADP-ribosyltransferases. Earlier evidence suggested that histidine-21 of DT is important for the ADP-ribosylation of eukaryotic elongation factor 2 (EF-2). We have generated substitutions of this residue by ca...

journal_title：Biochemistry

authors： Blanke SR,Huang K,Wilson BA,Papini E,Covacci A,Collier RJ

更新日期：1994-05-03 00:00:00