Zn(2+) ions selectively induce antimicrobial salivary peptide histatin-5 to fuse negatively charged vesicles. Identification and characterization of a zinc-binding motif present in the functional domain.

abstract：:The structures of ternary complexes of human inositol monophosphatase with inhibitory Gd3+ and either D- or L-myo-inositol 1-phosphate have been determined to 2.2-2.3 A resolution using X-ray crystallography. Substrate and metal are bound identically in each active site of the phosphatase dimer. The substrate is prese...

journal_title：Biochemistry

authors： Bone R,Frank L,Springer JP,Pollack SJ,Osborne SA,Atack JR,Knowles MR,McAllister G,Ragan CI,Broughton HB

更新日期：1994-08-16 00:00:00

abstract：:To elucidate early stages in protein folding, we have adopted a fragment reconstitution method for small proteins. This approach is expected to provide nuclei for protein folding and to allow us to investigate folding mechanisms. In previous work [Kobayashi, N., et al. (1995) FEBS Lett. 366, 99-103.] we demonstrated t...

journal_title：Biochemistry

authors： Honda S,Kobayashi N,Munekata E,Uedaira H

更新日期：1999-01-26 00:00:00

abstract：:It has previously been shown that 9S, untransformed progestin, estrogen, androgen, and glucocorticoid receptor complexes in rabbit uterine and liver cytosols contain a 59-kDa protein [Tai, P. K., Maeda, Y., Nakao, K., Wakim, N. G., Duhring, J. L., & Faber, L. E. (1986) Biochemistry 25, 5269-5275]. In this work we show...

journal_title：Biochemistry

authors： Sanchez ER,Faber LE,Henzel WJ,Pratt WB

更新日期：1990-05-29 00:00:00

abstract：:Locked nucleic acid (LNA) is a nucleic acid analogue containing one or more LNA nucleotide monomers with a bicyclic furanose unit locked in an RNA mimicking sugar conformation. LNA oligonucleotides display unprecedented hybridization affinity toward complementary single-stranded RNA and complementary single- or double...

journal_title：Biochemistry

authors： Vester B,Wengel J

更新日期：2004-10-26 00:00:00

abstract：:The smooth muscle basic calponin interacts with F-actin and inhibits the actomyosin ATPase in a calmodulin or phosphorylation modulated manner. It also binds in vitro to microtubules and its acidic isoform, present in nonmuscle cells, and co-localizes with microfilaments and microtubules in cultured neurons. To assess...

journal_title：Biochemistry

authors： Fattoum A,Roustan C,Smyczynski C,Der Terrossian E,Kassab R

更新日期：2003-02-11 00:00:00

abstract：:In halophilic archaea the photophobic response is mediated by the membrane-embedded 2:2 photoreceptor/-transducer complex SRII/HtrII, the latter being homologous to the bacterial chemoreceptors. Both systems bias the rotation direction of the flagellar motor via a two-component system coupled to an extended cytoplasmi...

journal_title：Biochemistry

authors： Orban-Glaß I,Voskoboynikova N,Busch KB,Klose D,Rickert C,Mosslehy W,Roder F,Wilkens V,Piehler J,Engelhard M,Steinhoff HJ,Klare JP

更新日期：2015-01-20 00:00:00

abstract：:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a glycolytic enzyme that also functions in transcriptional regulation, oxidative stress, vesicular trafficking, and apoptosis. Because GAPDH is required for the insertion of cellular heme into inducible nitric oxide synthase [Chakravarti, R., et al. (2010) Proc. Natl...

journal_title：Biochemistry

authors： Hannibal L,Collins D,Brassard J,Chakravarti R,Vempati R,Dorlet P,Santolini J,Dawson JH,Stuehr DJ

更新日期：2012-10-30 00:00:00

abstract：:In Escherichia coli, the cyclic AMP receptor protein (CRP) serves as a sensor of the intracellular level of cyclic AMP. At increasing concentrations of cyclic AMP, CRP becomes activated upon binding a cyclic AMP molecule. The activated CRP is capable of regulating the expression of more than 20 genes by binding to spe...

journal_title：Biochemistry

authors： Pyles EA,Chin AJ,Lee JC

更新日期：1998-04-14 00:00:00

abstract：:Aqueous suspensions of dark-adapted bacteriorhodopsin (bR560) in the purple membrane of Halobacterium halobium are exposed to rapid jumps to high pH. Optical and resonance Raman measurements are carried out by using flow and stationary methods. Above pH congruent to 11.5 bR560 starts to be reversibly converted to a sp...

journal_title：Biochemistry

authors： Druckmann S,Ottolenghi M,Pande A,Pande J,Callender RH

更新日期：1982-09-28 00:00:00

abstract：:Acyclovir triphosphate, ganciclovir triphosphate and penciclovir triphosphate inhibited DNA polymerases alpha, delta, and epsilon. Each triphosphate preferentially inhibited pol delta, although ganciclovir triphosphate was the most impressive of the three; the Ki for inhibition of pol delta was 2 microM (competitive w...

journal_title：Biochemistry

authors： Ilsley DD,Lee SH,Miller WH,Kuchta RD

更新日期：1995-02-28 00:00:00

abstract：:The quantitative tissue specificity of the high mobility group (HMG) chromosomal proteins was investigated. Perchloric acid (PCA) extracts of four different chicken tissues and erythrocytes contained three proteins which comigrated on NaDodSO4-polyacrylamide gels with the HMG's 1,2, and E from erythrocyte nuclei. Thes...

journal_title：Biochemistry

authors： Gordon JS,Rosenfeld BI,Kaufman R,Williams DL

更新日期：1980-09-16 00:00:00

abstract：:Calcium is required for the substrate binding and for the chemical step of the interfacial catalytic turnover cycle of pancreatic phospholipase A2 (PLA2), but not for the binding of the enzyme to the interface. The role of calcium and other divalent cations (C) is analyzed for the effect on the substrate binding and k...

journal_title：Biochemistry

authors： Yu BZ,Rogers J,Nicol GR,Theopold KH,Seshadri K,Vishweshwara S,Jain MK

更新日期：1998-09-08 00:00:00

abstract：:Human alpha1 and alpha2 isoforms of Na,K-ATPase have been expressed with porcine 10*Histidine-tagged beta1 subunit in Pichia pastoris. Methanol-induced expression of alpha2 is optimal at 20 degrees C, whereas at 25 degrees C, which is optimal for expression of alpha1, alpha2 is not expressed. Detergent-soluble alpha2b...

journal_title：Biochemistry

authors： Lifshitz Y,Petrovich E,Haviv H,Goldshleger R,Tal DM,Garty H,Karlish SJ

更新日期：2007-12-25 00:00:00

abstract：:Efforts to elucidate the nature of the bimolecular interaction of parathyroid hormone (PTH) with its cognate receptor, the PTH receptor type 1 (PTHR1), have relied heavily on benzoylphenylalanine- (Bpa-) based photoaffinity cross-linking. However, given the flexibility, size, and shape of Bpa, the resolution at the PT...

journal_title：Biochemistry

authors： Monaghan P,Thomas BE,Woznica I,Wittelsberger A,Mierke DF,Rosenblatt M

更新日期：2008-06-03 00:00:00

abstract：:At pH 5.5, binding of bleomycin relaxed supercoiled ColE1 DNA without breaking it. Binding of tripeptide S, a fragment of the drug containing the bithiazole rings, also relaxed and then recoiled supercoiled DNA, at pH 5.5 and at pH 8.0, where bleomycin is normally active. The unwinding angle was 12 degrees. Both compo...

journal_title：Biochemistry

authors： Povirk LF,Hogan M,Dattagupta N

更新日期：1979-01-09 00:00:00

abstract：:Cyanobacteriochromes (CBCRs) are cyanobacterial photoreceptors that exhibit photochromism between two states: a thermally stable dark-adapted state and a metastable light-adapted state with bound linear tetrapyrrole (bilin) chromophores possessing 15Z and 15E configurations, respectively. The photodynamics of canonica...

journal_title：Biochemistry

authors： Kirpich JS,Chang CW,Franse J,Yu Q,Escobar FV,Jenkins AJ,Martin SS,Narikawa R,Ames JB,Lagarias JC,Larsen DS

更新日期：2021-01-13 00:00:00

abstract：:Thermococcus litoralis 4-alpha-glucanotransferase (TLGT) belongs to family 57 of glycoside hydrolases and catalyzes the disproportionation and cycloamylose synthesis reactions. Family 57 glycoside hydrolases have not been well investigated, and even the catalytic mechanism involving the active site residues has not be...

journal_title：Biochemistry

authors： Imamura H,Fushinobu S,Jeon BS,Wakagi T,Matsuzawa H

更新日期：2001-10-16 00:00:00

abstract：:Urea-induced unfolding of Na,K-ATPase from pig kidney and from shark salt gland was studied by electron paramagnetic resonance (EPR) spectroscopy of a nitroxyl derivative of maleimide covalently attached to sulfhydryl groups which are essential for activity. Urea-induced structural changes lead to the inhibition of Na...

journal_title：Biochemistry

authors： Babavali M,Esmann M,Fedosova NU,Marsh D

更新日期：2009-09-29 00:00:00

abstract：:Pulmonary surfactant forms a monolayer of lipids and proteins at the alveolar air/liquid interface. Although cholesterol is a natural component of surfactant, its function in surface dynamics is unclear. To further elucidate the role of cholesterol in surfactant, we used a captive bubble surfactometer (CBS) to measure...

journal_title：Biochemistry

authors： Diemel RV,Snel MM,Van Golde LM,Putz G,Haagsman HP,Batenburg JJ

更新日期：2002-12-17 00:00:00

abstract：:Charybdotoxin (ChTX) and iberiotoxin (IbTX) are highly charged peptidyl toxins which exhibit 68% sequence identity and share a similar three-dimensional structure. Despite these structural similarities, IbTX and ChTX differ in their selectivity for two types of potassium channels; large conductance calcium-activated p...

journal_title：Biochemistry

authors： Giangiacomo KM,Sugg EE,Garcia-Calvo M,Leonard RJ,McManus OB,Kaczorowski GJ,Garcia ML

更新日期：1993-03-09 00:00:00

abstract：:The iron-chelating peptide vibriobactin of the pathogenic Vibrio cholerae is assembled by a four-subunit nonribosomal peptide synthetase complex, VibE, VibB, VibH, and VibF, using 2,3-dihydroxybenzoate and L-threonine as precursors to two 2,3-dihydroxyphenyl- (DHP-) methyloxazolinyl groups in amide linkage on a norspe...

journal_title：Biochemistry

authors： Marshall CG,Burkart MD,Keating TA,Walsh CT

更新日期：2001-09-04 00:00:00

abstract：:The metabolism of the anticonvulsant mephenytoin is subject to a genetic polymorphism. In 2-5% of Caucasians and 18-23% of Japanese subjects a specific cytochrome P-450 isozyme, P-450 meph, is functionally deficient or missing. We have accumulated evidence that autoimmune antibodies observed in sera of patients with t...

journal_title：Biochemistry

authors： Meier UT,Meyer UA

更新日期：1987-12-15 00:00:00

abstract：:Clip domains are structural modules found in arthropod serine proteinases and some proteolytically inactive homologues, which mediate extracellular signaling pathways of development and immunity. While little is known about their structures or functions, clip domains are proposed to be sites for interactions of protei...

journal_title：Biochemistry

authors： Huang R,Lu Z,Dai H,Velde DV,Prakash O,Jiang H

更新日期：2007-10-16 00:00:00

abstract：:The synaptotagmin (Syt) family of proteins plays an important role in vesicle docking and fusion during Ca(2+)-induced exocytosis in a wide variety of cell types. Its role as a Ca(2+) sensor derives primarily from its two C2 domains, C2A and C2B, which insert into anionic lipid membranes upon binding Ca(2+). Syt isofo...

journal_title：Biochemistry

authors： Osterberg JR,Chon NL,Boo A,Maynard FA,Lin H,Knight JD

更新日期：2015-09-22 00:00:00

abstract：:Conformational and environmental changes in the functionally significant amino-terminal region of human parathyroid hormone (hPTH), induced by solvent or by complexation with acidic lipid, have been investigated. Structural perturbations were monitored by their effect on the fluorescence decay kinetics of the single t...

journal_title：Biochemistry

authors： Willis KJ,Szabo AG

更新日期：1992-09-22 00:00:00

abstract：:The phosphorylating of the lysine-rich histone at various stages in the cell cycle has been studied. In rapidly dividing cell populations the lysine-rich histone is phosphorylated rapidly after synthesis and more slowly once bound to the chromosome. The half-life of hydrolysis of such interphase phosphorylation in 5 h...

journal_title：Biochemistry

authors： Balhorn R,Jackson V,Granner D,Chalkey R

更新日期：1975-06-03 00:00:00

abstract：:Lateral mobilities of fluorescent cell surface probes have been measured on normal (3T3) and transformed (SV3T3) cultured mouse fibroblasts. There is little discernible difference in the mobilities of a lipid analogue (diI), a fluorescent ganglioside derivative (GM1), and tetramethylrhodamine-labeled succinylated conc...

journal_title：Biochemistry

authors： Eldridge CA,Elson EL,Webb WW

更新日期：1980-05-13 00:00:00

abstract：:The isothermal equilibrium folding of the unmodified yeast tRNA(Phe) is studied as a function of Na(+), Mg(2+), and urea concentration with hydroxyl radical protection, circular dichroism, and diethyl pyrocarbonate (DEPC) modification. These assays indicate that this tRNA folds in Na(+) alone. Similar to folding in Mg...

journal_title：Biochemistry

authors： Shelton VM,Sosnick TR,Pan T

更新日期：2001-03-27 00:00:00

abstract：:The structure and folding of a novel human insulin mutant, [Thr(B27) --> Pro, Pro(B28) --> Thr]insulin (PT insulin), in aqueous solution and in mixtures of water and 2,2,2-trifluoroethanol (TFE) have been studied by NMR spectroscopy. It was found that PT insulin has a highly flexible structure in pure water and is pre...

journal_title：Biochemistry

authors： Keller D,Clausen R,Josefsen K,Led JJ

更新日期：2001-09-04 00:00:00

abstract：:The oxycomplexes (compound III, oxyperoxidase) of two lignin peroxidase isozymes, H1 (pI = 4.7) and H8 (pI = 3.5), were characterized in the present study. After generation of the ferroperoxidase by photochemical reduction with deazoflavin in the presence of EDTA, the oxycomplex is formed by mixing ferroperoxidase wit...

journal_title：Biochemistry

authors： Cai DY,Tien M

更新日期：1990-02-27 00:00:00