Abstract:
:Information on the secondary structure and interactions of RNA is important to understand the biological function of RNA as well as in applying RNA as a tool for therapeutic purposes. Recently, the isoenergetic microarray mapping method was developed to improve the prediction of RNA secondary structure. Herein, for the first time, isoenergetic microarrays were used to study the binding of RNA to protein or other RNAs as well as the interactions of two different RNAs and protein in a three-component complex. The RNAs used as models were the regulatory DsrA and OxyS RNAs from Escherichia coli, the fragments of their target mRNAs (fhlA and rpoS), and their complexes with Hfq protein. The collected results showed the advantages and some limitations of microarray mapping.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Fratczak A,Kierzek R,Kierzek Edoi
10.1021/bi200463psubject
Has Abstractpub_date
2011-09-06 00:00:00pages
7647-65issue
35eissn
0006-2960issn
1520-4995journal_volume
50pub_type
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