Abstract:
:Misaligned structures can result from strand slippage during DNA replication and, if not repaired, would lead to mutations. Previously, we showed that strand slippage can occur upon misincorporation of a dNTP opposite thymine and cytosine templates, resulting in a misaligned structure with a T- or C-bulge. The formation propensity for misaligned structures was found to depend on the type of terminal base pair. In this study, we performed NMR investigations on primer-template models containing a guanine template. Our results reveal guanine templates are less prone to strand slippage than pyrimidine templates. Misalignment was found to occur only in 5'-CG templates with a downstream purine. In addition to the significance of terminal base pair and upstream nucleotide, the present study reveals the importance of the templating base and its downstream nucleotide, which also determine the propensity of strand slippage in primer-templates.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Chi LM,Lam SLdoi
10.1021/bi9014049subject
Has Abstractpub_date
2009-12-08 00:00:00pages
11478-86issue
48eissn
0006-2960issn
1520-4995journal_volume
48pub_type
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