当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Intermediates in excision repair by human cells: use of S1 nuclease and benzoylated naphthoylated cellulose to reveal single-strand breaks.

Intermediates in excision repair by human cells: use of S1 nuclease and benzoylated naphthoylated cellulose to reveal single-strand breaks.

Abstract：

:Treatment of human lymphoid cells with methyl methanesulfonate (MMS) results in discontinuities in cellular DNA. Discontinuities can be detected by sedimentation through neutral sucrose gradients after digestion with S1 nuclease on top of the gradient and by benzoylated naphthoylated diethylaminoethylcellulose (BND-cellulose) chromatography. DNA with discontinuities is sensitive to shear which produces single-stranded regions and causes the DNA to adhere to BND-cellulose. The shear-sensitive and S1-sensitive sites produced by MMS treatment are probably identical. Treatment of cells with acetoxyacetylaminofluorene (AAAF) does not result in detectable discontinuities either at doses which produce the same number of adducts as observed after MMS treatment or at doses which inhibit DNA excision repair. Addition of aromatic adducts to DNA can also result in adherence to BND-cellulose but such DNA is not S1 sensitive. We conclude that MMS- and AAAF-induced damage are repaired by different pathways in human cells and that DNA with discontinuities is a readily detectable intermediate only in the repair of MMS damage by the apurinic pathway.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Karran P,Higgins NP,Strauss B

doi

10.1021/bi00639a024

subject

Has Abstract

pub_date

1977-10-04 00:00:00

pages

4483-90

issue

20

eissn

0006-2960

issn

1520-4995

journal_volume

16

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • tRNA discrimination at the binding step by a class II aminoacyl-tRNA synthetase.

    abstract：:Aminoacyl-tRNA synthetases preserve the fidelity of decoding genetic information by accurately joining amino acids to their cognate transfer RNAs. Here, tRNA discrimination at the level of binding by Escherichia coli histidyl-tRNA synthetase is addressed by filter binding, analytical ultracentrifugation, and iodine fo...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi991182g

    authors： Bovee ML,Yan W,Sproat BS,Francklyn CS

    更新日期：1999-10-12 00:00:00

  • Characterization of human aspartoacylase: the brain enzyme responsible for Canavan disease.

    abstract：:Aspartoacylase catalyzes the deacetylation of N-acetylaspartic acid (NAA) to produce acetate and L-aspartate and is the only brain enzyme that has been shown to effectively metabolize NAA. Although the exact role of this enzymatic reaction has not yet been completely elucidated, the metabolism of NAA appears to be nec...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi052608w

    authors： Le Coq J,An HJ,Lebrilla C,Viola RE

    更新日期：2006-05-09 00:00:00

  • A test of the linear extrapolation of unfolding free energy changes over an extended denaturant concentration range.

    abstract：:Guanidine hydrochloride (GdnHCl) and thermally induced unfolding measurements on the oxidized form of Escherichia coli thioredoxin at pH 7 were combined for the purpose of assessing the functional dependence of unfolding free energy changes on denaturant concentration over an extended GdnHCl concentration range. Conve...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00135a022

    authors： Santoro MM,Bolen DW

    更新日期：1992-05-26 00:00:00

  • Temperature-dependent CD and NMR studies on a synthetic oligonucleotide containing a B-Z junction at high salt.

    abstract：:It is now accepted that two or more conformations may exist within the same DNA molecule, thereby generating conformational junctions. The presence of B-Z junctions between right- and left-handed DNA conformations has been detected in plasmids by a number of techniques. Preliminary characterization of the first exampl...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00428a046

    authors： Sheardy RD,Winkle SA

    更新日期：1989-01-24 00:00:00

  • Geometry of the phosphodiester backbone in the A form of deoxyribonucleic acid determined by phosphorus-31 nuclear magnetic resonance spectroscopy.

    abstract：:31P NMR spectra were obtained from poly(dA-dT) . poly(dA-dT) fibers which gave an X-ray diffraction pattern similar to that of the A form of natural deoxyribonucleic acid (DNA). The analysis of the line shape indicated that the A form of poly(dA-dT) . poly(dA-dT) has a single uniform backbone conformation; the orienta...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00507a013

    authors： Shindo H,Wooten JB,Zimmerman SB

    更新日期：1981-02-17 00:00:00

  • Using saturation-recovery EPR to measure distances in proteins: applications to photosystem II.

    abstract：:The stable tyrosine radical YD. (tyrosine 160 in the D2 polypeptide) in photosystem II (PSII) exhibits nonexponential electron spin-lattice relaxation transients at low temperature. As previously reported, the tetranuclear Mn complex in PSII significantly enhances the spin-lattice relaxation of YD.. However, in Mn-dep...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00117a033

    authors： Hirsh DJ,Beck WF,Innes JB,Brudvig GW

    更新日期：1992-01-21 00:00:00

  • Amino acid 305 determines catalytic center accessibility in CYP3A4.

    abstract：:Site-directed mutagenesis has been used to replace alanine 305 with phenylalanine (A305F) and serine (A305S) in the active site of cytochrome P450 3A4 (CYP3A4). Enzyme kinetics for diazepam, erythromycin, nifedipine, and testosterone metabolism have been determined for both mutants and wild-type CYP3A4. The A305F muta...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi992372u

    authors： Fowler SM,Riley RJ,Pritchard MP,Sutcliffe MJ,Friedberg T,Wolf CR

    更新日期：2000-04-18 00:00:00

  • Secondary structure of human interleukin 2 from 3D heteronuclear NMR experiments.

    abstract：:Recombinant 15N-labeled human interleukin 2 (IL-2) has been studied by 2D and 3D NMR using uniformly 15N-labeled protein. Assignment of the backbone resonances has enabled the secondary structure of the protein to be defined. The secondary structure was found to consist of four alpha-helical regions and a short sectio...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00148a040

    authors： Mott HR,Driscoll PC,Boyd J,Cooke RM,Weir MP,Campbell ID

    更新日期：1992-08-25 00:00:00

  • Assignment of spectral substructures to pigment-binding sites in higher plant light-harvesting complex LHC-II.

    abstract：:The trimeric main light-harvesting complex (LHC-II) is the only antenna complex of higher plants of which a high-resolution 3D structure has been obtained (Kühlbrandt, W., Wang, D., and Fujiyoshi, Y. (1994) Nature 367, 614-621) and which can be refolded in vitro from its components. Four different recombinant forms of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi015875k

    authors： Rogl H,Schödel R,Lokstein H,Kühlbrandt W,Schubert A

    更新日期：2002-02-19 00:00:00

  • An unnatural folate stereoisomer is catalytically competent in DNA photolyase.

    abstract：:The folate chromophore in native Escherichia coli DNA photolyase ([6R]-5,10-CH+-H4Pte(Glu)n=3-6) serves as an antenna, transferring light energy to the fully reduced flavin (FADH2) reaction center at high efficiency (EET = 0.92). Apophotolyase reconstituted after an overnight incubation with [6R,S]-5,10-CH+-H4folate (...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9605548

    authors： Lipman RS,Jorns MS

    更新日期：1996-06-18 00:00:00

  • Primary photoreaction of photoactive yellow protein studied by subpicosecond-nanosecond spectroscopy.

    abstract：:The primary photochemical event of photoactive yellow protein (PYP) was studied by laser flash photolysis experiments on a subpicosecond-nanosecond time scale. PYP was excited by a 390-nm pulse, and the transient difference absorption spectra were recorded by a multichannel spectrometer for a more reliable spectral an...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi002437p

    authors： Imamoto Y,Kataoka M,Tokunaga F,Asahi T,Masuhara H

    更新日期：2001-05-22 00:00:00

  • The imine-pyridine torsion of the pyridoxal 5'-phosphate Schiff base of aspartate aminotransferase lowers its pKa in the unliganded enzyme and is crucial for the successive increase in the pKa during catalysis.

    abstract：:In aspartate aminotransferase, pyridoxal 5'-phosphate (PLP) forms a Schiff base with the epsilon-amino group of Lys258 (internal aldimine). The internal aldimine has a pKa value of 6.8. Binding of a substrate amino acid to the enzyme yields the Michaelis complex, in which PLP still forms the internal aldimine with Lys...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi981517e

    authors： Hayashi H,Mizuguchi H,Kagamiyama H

    更新日期：1998-10-27 00:00:00

  • Purification and characterization of the human erythrocyte band 3 protein C-terminal domain.

    abstract：:To clarify the function of the hydrophilic carboxyl-terminal tail of human erythrocyte membrane band 3 protein (HEM-B3), we purified two peptides, C1 (Ala893-Val911) and KS4 (Gly647-Arg656), from human erythrocyte band 3 protein preparations. Purified C1 peptides at concentrations from 5 to 80 microM were incubated wi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi035281c

    authors： Fu G,Wang T,Yang B,Lv F,Shi C,Jiang X,Tian L,Yu W,Hamasaki N

    更新日期：2004-02-17 00:00:00

  • Prothrombin domains: circular dichroic evidence for a lack of cooperativity.

    abstract：:The far-ultraviolet circular dichroism spectra of bovine and human prothrombin, prothrombin fragment 1, prethrombin 1, prothrombin fragment 2, and prethrombin 2 (prethrombin 2des(1--13)) were determined and the method of Chen et al. [Chen, Y. H., Yang, J. T., & Martinez, H. M. (1972) Biochemistry 11, 4120--4131; Chen,...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00577a017

    authors： Bloom JW,Mann KG

    更新日期：1979-05-15 00:00:00

  • A "Seleno Effect" Differentiates the Roles of Redox Active Cysteine Residues in Plasmodium falciparum Thioredoxin Reductase.

    abstract：:Here, we introduce the concept of the "seleno effect" in the study of oxidoreductases that catalyze thiol/disulfide exchange reactions. In these reactions, selenium can replace sulfur as a nucleophile, electrophile, or leaving group, and the resulting change in rate (the seleno effect) is defined as kS/ kSe. In soluti...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b00004

    authors： O'Keefe JP,Dustin CM,Barber D,Snider GW,Hondal RJ

    更新日期：2018-03-20 00:00:00

  • Deuterium exchange of operator 8CH groups as a Raman probe of repressor recognition: interactions of wild-type and mutant lambda repressors with operator OL1.

    abstract：:The rate of deuterium exchange of a purine 8CH group in DNA is highly sensitive to both macromolecular secondary structure and intermolecular interactions which restrict solvent access to the major groove [Lamba, O.P., Becka, R., & Thomas, G.J., Jr. (1990) Biopolymers 29, 1465-1477]. We have exploited the sensitivity ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00127a012

    authors： Reilly KE,Becka R,Thomas GJ Jr

    更新日期：1992-03-31 00:00:00

  • Tubulin-G protein association stabilizes GTP binding and activates GTPase: cytoskeletal participation in neuronal signal transduction.

    abstract：:It has been suggested that dimeric tubulin can participate in the signal transduction process through its association with the GTP-binding (G) proteins Gs and Gi1. Using the photoaffinity GTP analog, azidoanilido-GTP, it has been shown that the transfer of nucleotide from tubulin to G alpha s and G alpha i1 is the key...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00198a052

    authors： Roychowdhury S,Rasenick MM

    更新日期：1994-08-16 00:00:00

  • Transducer-binding and transducer-mutations modulate photoactive-site-deprotonation in sensory rhodopsin I.

    abstract：:Sensory rhodopsin I (SRI) is a seven-transmembrane helix retinylidene protein that mediates color-sensitive phototaxis responses through its bound transducer HtrI in the archaeon Halobacterium salinarum. Deprotonation of the Schiff base attachment site of the chromophore accompanies formation of the SRI signaling stat...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi991180w

    authors： Jung KH,Spudich EN,Dag P,Spudich JL

    更新日期：1999-10-05 00:00:00

  • The Ebola Viral Protein 35 N-Terminus Is a Parallel Tetramer.

    abstract：:Members of Mononegavirales, the order that includes nonsegmented negative sense RNA viruses (NNSVs), encode a small number of multifunctional proteins. In members of the Filoviridae family, virus protein 35 (VP35) facilitates immune evasion and functions as an obligatory cofactor for viral RNA synthesis. VP35 function...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b01154

    authors： Chanthamontri CK,Jordan DS,Wang W,Wu C,Lin Y,Brett TJ,Gross ML,Leung DW

    更新日期：2019-02-12 00:00:00

  • Ability of DNA and spermidine to affect the activity of restriction endonucleases from several bacterial species.

    abstract：:Previous work has described the novel ability to modulate in vitro the activity of restriction endonuclease NaeI from Nocardia aerocoligenes by using cleavable DNA and spermidine [Conrad & Topal (1989) Proc. Natl. Acad. Sci. U.S.A. 86, 9707-9711]. In this paper we report the results of a study of 49 type II restrictio...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00223a035

    authors： Oller AR,Vanden Broek W,Conrad M,Topal MD

    更新日期：1991-03-05 00:00:00

  • Pyrrolo[1,4]benzodiazepine antibiotics. Biosynthesis of the antitumor antibiotic sibiromycin by Streptosporangium sibiricum.

    abstract：:The biosynthesis of the antitumor antibiotic sibiromycin by Streptosporangium sibiricum requires the construction of four units: the amino sugar from glucose; the anthranilate ring from DL-tryptophan probably via kynurenine; the aromatic methyl group from methionine; the propylidene proline from L-tyrosine with the lo...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00586a029

    authors： Hurley LH,Lasswell WL,Malhotra RK,Das NV

    更新日期：1979-09-18 00:00:00

  • Induced alpha-helix structure in the aryl hydrocarbon receptor transactivation domain modulates protein-protein interactions.

    abstract：:The aryl hydrocarbon receptor (AhR) is an intracellular receptor protein that regulates gene transcription in response to both man-made and natural ligands. A modular transactivaton domain (TAD) has been mapped to the 304 C-terminal amino acids and consists of acidic, Q-rich, and P/S/T-rich subdomains. We have used st...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0487701

    authors： Watt K,Jess TJ,Kelly SM,Price NC,McEwan IJ

    更新日期：2005-01-18 00:00:00

  • Cofactor Complexes of DesD, a Model Enzyme in the Virulence-related NIS Synthetase Family.

    abstract：:The understudied nonribosomal-peptide-synthetase-independent siderophore (NIS) synthetase family has been increasingly associated with virulence in bacterial species due to its key role in the synthesis of hydroxamate and carboxylate "stealth" siderophores. We have identified a model family member, DesD, from Streptom...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.9b00899

    authors： Hoffmann KM,Goncuian ES,Karimi KL,Amendola CR,Mojab Y,Wood KM,Prussia GA,Nix J,Yamamoto M,Lathan K,Orion IW

    更新日期：2020-09-22 00:00:00

  • Composition and structure of zinc-deficient Euglena gracilis chromatin.

    abstract：:The histone content of zinc-deficient (-Zn) Euglena gracilis decreases while, concomitantly, DNA content increases and the transcription rate is reduced markedly [Mazus, B., Falchuk, K. H., & Vallee, B. L. (1983) Biochemistry (in press); Falchuk, K. H., Fawcett, D. W., & Vallee, B. L. (1975) J. Cell Sci. 17, 57-78]. T...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00291a014

    authors： Stankiewicz AJ,Falchuk KH,Vallee BL

    更新日期：1983-10-25 00:00:00

  • Arginine-42 and threonine-45 are required for FAD incorporation and catalytic activity in human monoamine oxidase B.

    abstract：:Monoamine oxidase B (MAO B) is an integral protein of the outer mitochondrial membrane that is involved in the deamination of vasoactive and neuroactive amines. The oxidation of these amine substrates requires the cofactor FAD, which is covalently bound to Cys-397 of human MAO B. Previously, Glu-34 and Tyr-44 of MAO B...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9806910

    authors： Kirksey TJ,Kwan SW,Abell CW

    更新日期：1998-09-01 00:00:00

  • Identifying the lowest electronic states of the chlorophylls in the CP47 core antenna protein of photosystem II.

    abstract：:CP47 is a pigment-protein complex in the core of photosystem II that tranfers excitation energy to the reaction center. Here we report on a spectroscopic investigation of the isolated CP47 complex. By deconvoluting the 77 K absorption and linear dichroism, red-most states at 683 and 690 nm have been identified with os...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0261948

    authors： De Weerd FL,Palacios MA,Andrizhiyevskaya EG,Dekker JP,Van Grondelle R

    更新日期：2002-12-24 00:00:00

  • Long-range interaction networks in the function and fidelity of poliovirus RNA-dependent RNA polymerase studied by nuclear magnetic resonance.

    abstract：:The fidelity of the poliovirus RNA-dependent RNA polymerase (3D(pol)) plays a direct role in the genomic evolution and pathogenesis of the virus. A single site mutation (Gly64Ser) that is remote from the catalytic center results in a higher fidelity polymerase. NMR studies with [methyl-(13)C]methionine-labeled protein...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi100833r

    authors： Yang X,Welch JL,Arnold JJ,Boehr DD

    更新日期：2010-11-02 00:00:00

  • Engineering a novel specificity in subtilisin BPN'.

    abstract：:The specificity of subtilisin BPN' toward substrates with large hydrophobic P4 residues has been improved by single amino acid replacements at positions 104 and 107. Mutations were designed to (i) increase the size of the P4 binding pocket by replacing Ile107, which is at the bottom of the S4 pocket, by Val, Ala, and ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00056a001

    authors： Rheinnecker M,Baker G,Eder J,Fersht AR

    更新日期：1993-02-09 00:00:00

  • Pre-steady-state kinetic analysis of enzyme-monitored turnover during cystathionine β-synthase-catalyzed H(2)S generation.

    abstract：:Cystathionine β-synthase (CBS) catalyzes the first step in the transsulfuration pathway in mammals, i.e., the condensation of serine and homocysteine to produce cystathionine and water. Recently, we have reported a steady-state kinetic analysis of the three hydrogen sulfide (H(2)S)-generating reactions that are cataly...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi1010893

    authors： Singh S,Ballou DP,Banerjee R

    更新日期：2011-01-25 00:00:00

  • Mycoplasma gallisepticum produces a histone-like protein that recognizes base mismatches in DNA.

    abstract：:Mycoplasmas are the smallest known microorganisms, with drastically reduced genome sizes. One of the essential biochemical pathways lost in mycoplasmas is methylation-mediated DNA repair (MMR), which is responsible for correction of base substitutions, insertions, and deletions in both bacteria and higher organisms. W...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi2009097

    authors： Kamashev D,Oberto J,Serebryakova M,Gorbachev A,Zhukova Y,Levitskii S,Mazur AK,Govorun V

    更新日期：2011-10-11 00:00:00