Abstract：
:The primary photochemical event of photoactive yellow protein (PYP) was studied by laser flash photolysis experiments on a subpicosecond-nanosecond time scale. PYP was excited by a 390-nm pulse, and the transient difference absorption spectra were recorded by a multichannel spectrometer for a more reliable spectral analysis than previously possible. Just after excitation, an absorbance decrease due to the stimulated emission at 500 nm and photoconversion of PYP at 450 nm were observed. The stimulated emission gradually shifted to 520 nm and was retained up to 4 ps. Then, the formation of a red-shifted intermediate with a broad absorption spectrum was observed from 20 ps to 1 ns. Another red-shifted intermediate with a narrow absorption spectrum was formed after 2 ns and was stable for at least 5 ns. The latter is therefore believed to correspond to I1 (PYP(L)), which has been detected on a nanosecond time scale or trapped at -80 degrees C. Singular value decomposition analysis demonstrated that the spectral shifts observed from 0.5 ps to 5 ns could be explained by two-component decay of excited state(s) and conversion from PYP(B) to PYP(L). The amount of PYP(L) at 5 ns was less than that of photoconverted PYP, suggesting the formation of another intermediate, PYP(H). In addition, the absorption spectra of these intermediates were calculated based on the proposed reaction scheme. Together, these results indicate that the photocycle of PYP at room temperature has a branched pathway in the early stage and is essentially similar to that observed under low-temperature spectroscopy.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Imamoto Y,Kataoka M,Tokunaga F,Asahi T,Masuhara Hdoi
10.1021/bi002437psubject
Has Abstractpub_date
2001-05-22 00:00:00pages
6047-52issue
20eissn
0006-2960issn
1520-4995pii
bi002437pjournal_volume
40pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:A combined experimental and theoretical investigation of the deuterium isotope effects on the bacterial luciferase reaction is described. The experimental studies focus on determining if the unusual aldehydic deuterium isotope effect of approximately 1.5 observed in these reactions is an intrinsic isotope effect resul...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972266x
更新日期：1998-02-24 00:00:00
abstract：:Isolated chloroplasts are capable of synthesizing chloroplast DNA in the presence of Mg2+ and deoxynucleoside triphosphates. The in vitro reaction proceeds for at least 60 min and is inhibited by KC1 and N-ethylmaleimide. Stretches of several hundred nucleotides in length are synthesized within an hour. Little or no i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00257a014
更新日期：1982-07-06 00:00:00
abstract：:Beta-1,4-galactosyltransferase (beta4Gal-T1) in the presence of manganese ion transfers galactose from UDP-galactose (UDP-Gal) to N-acetylglucosamine (GlcNAc) that is either free or linked to an oligosaccharide. Crystallographic studies on bovine beta4Gal-T1 have shown that the primary metal binding site is located in...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049007+
更新日期：2004-10-05 00:00:00
abstract：:Despite significant progress in the elucidation of the genetic basis of early-onset familial Alzheimer's disease (AD), the etiology of sporadic cases remains elusive. Although certain genetic loci play a role in conferring susceptibility in some sporadic AD cases, it is likely that the etiology is multifactorial; henc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi000029f
更新日期：2000-05-23 00:00:00
abstract：:Quinolinate phosphoribosyltransferase (QAPRTase, EC 2.4.2.19) catalyzes the formation of nicotinate mononucleotide, carbon dioxide, and pyrophosphate from 5-phosphoribosyl 1-pyrophosphate (PRPP) and quinolinic acid (QA, pyridine 2,3-dicarboxylic acid). The enzyme is the only type II PRTase whose X-ray structure is kno...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi012148g
更新日期：2002-03-12 00:00:00
abstract：:HTC cells have been labeled by short exposures to [3H]thymidine in order to identify newly synthesized DNA. By either isolating nuclei directly or isolating them after an extensive fixation with formaldehyde, we have been able to identify two phases in the maturation process of newly replicated chromatin. The first ph...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00302a036
更新日期：1984-03-27 00:00:00
abstract：:Histone redistributions between endogenous DNA in calf thymus chromatin and exogenous DNA from Clostridium perfringens (69% A + T) or from Micrococcus luteus (30% A + T) induced by 0.6 M NaCl or by 2% formaldehyde were studied by thermal denaturation. The observed redistribution occurred on histone Hl when the exogeno...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00666a002
更新日期：1976-10-19 00:00:00
abstract：:The allosteric interactions that regulate substrate channeling and catalysis in the tryptophan synthase bienzyme complex from Salmonella typhimurium are triggered by covalent reactions at the beta-site and binding of substrate/product to the alpha-site. The transmission of these allosteric signals between the alpha- a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034291a
更新日期：2003-07-01 00:00:00
abstract：:A gentle and rapid isolation procedure is described yielding fractions containing better than 95% pure OmpF porin of Escherichia coli BE with different amounts of bound lipopolysaccharide (LPS). The procedure employs continuous free-flow electrophoresis (FFE) in the presence of detergent above its critical micelle con...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00436a010
更新日期：1989-05-16 00:00:00
abstract：:A substrate analogue, 6-(difluoromethyl)tryptophan, was developed and characterized for mechanistic investigation of tryptophanase. The utility of this derivative was based on its ability to partition between fluoride elimination and carbon-carbon bond scission during tryptophan metabolism. The non-enzymatic hydrolysi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00221a018
更新日期：1991-02-19 00:00:00
abstract：:Redox transitions in the Rhodobacter capsulatus cytochrome bc(1) complex were investigated by perfusion-induced attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy combined with synchronous visible spectroscopy, in both the wild type and a cytochrome c(1) point mutant, M183K, in which the mi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049211x
更新日期：2004-07-27 00:00:00
abstract：:Anatoxin-a and homoanatoxin-a are two potent cyanobacterial neurotoxins. We recently reported the identification of the gene cluster responsible for the biosynthesis of these toxins as well as the in-vitro reconstitution of the first steps of this biosynthesis. We now report experimental evidence supporting the propos...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200892a
更新日期：2011-08-23 00:00:00
abstract：:Rapid withdrawal of estrogen from immature chicks, previously stimulated with the hormone, results in the inhibition of transcription of mRNAs of egg white proteins, rapid degradation of existing estrogen-induced mRNAs of egg white proteins, and decline in ribosomes and weight of the oviduct. On rapid withdrawal of es...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00409a019
更新日期：1988-05-03 00:00:00
abstract：:Hypoxic respiratory diseases or hypoxia exposures are frequently accompanied by glucose intolerance and impaired nitric oxide (NO) availability. However, the molecular mechanism responsible for impaired NO production and insulin resistance (IR) during hypoxia remains obscure. In this study, we investigated the possibl...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi401061e
更新日期：2014-01-14 00:00:00
abstract：:Troponin T (TnT) is an essential element in the thin filament-based regulatory system of striated muscle. Alternative mRNA splicing generates multiple TnT isoforms with primary structural differences in the NH2-terminal region. The functional significance of this hypervariable NH2-terminal domain and the developmental...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9812322
更新日期：1998-10-13 00:00:00
abstract：:4-Azido-2-nitrophenyl phosphate (ANPP) was synthesized and characterized. ANPP, unlabeled or labeled by 32P, was used as a photoreactive analogue of Pi to study the Pi binding site(s) in isolated F1-ATPase and inside-out particles from beef heart mitochondria. In the dark, the phosphate bond of ANPP was cleaved by alk...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00561a013
更新日期：1980-09-30 00:00:00
abstract：:The reaction of nitric oxide (NO) with fast cytochrome bo from Escherichia coli has been studied by electronic absorption, MCD, and EPR spectroscopy. Titration of the enzyme with NO showed the formation of two distinct species, consistent with NO binding stoichiometries of 1:1 and 2:1 with observed dissociation consta...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971481a
更新日期：1997-12-23 00:00:00
abstract：:Bacteriocins are bacterial peptides with specific activity against competing species. They hold great potential as natural preservatives and for their probiotic effects. We show here nuclear magnetic resonance-based evidence that glycocin F, a 43-amino acid bacteriocin from Lactobacillus plantarum, contains two β-link...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200217u
更新日期：2011-04-12 00:00:00
abstract：:Unilamellar liposomes can be fused at low pH with the plasma membrane of cells that express the hemagglutinin glycoprotein of influenza virus on their surface [van Meer, G., & Simons, K. (1983) J. Cell Biol. 97, 1365-1374]. Here, we have resolved this fusion process into two kinetically distinct steps. The first and m...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00335a030
更新日期：1985-07-02 00:00:00
abstract：:Various omega-halogenated carboxy acids and amides were evaluated as potential active-site-directed reagents for alcohol dehydrogenase. 2-Bromoacetamide and bromoacetic and 3-bromopropionic acids inactivated the enzyme; AMP, NAD+, and NADH markedly decreased the rate of inactivation. Some omega-halogenated carboxyamid...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00694a034
更新日期：1975-11-18 00:00:00
abstract：:In contrast to FK506 binding proteins and cyclophilins, the parvulin family of peptidyl-prolyl cis/trans isomerases (PPIases; E.C. 5.2.1.8) cannot be inhibited by either FK506 or cyclosporin A. We have found that juglone, 5-hydroxy-1,4-naphthoquinone, irreversibly inhibits the enzymatic activity of several parvulins, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi973162p
更新日期：1998-04-28 00:00:00
abstract：:The ligation state of hemoglobin during its cross-linking by glycolaldehyde influences the ultimate oxygen affinity of the cross-linked protein. Thus, if the cross-linking is performed with carbonmonoxy-hemoglobin, the oxygen affinity increases slightly to a P50 of 7 mmHg from a P50 of 9 mmHg for unmodified hemoglobin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00417a064
更新日期：1988-08-23 00:00:00
abstract：:The structural factors responsible for the extraordinary rate enhancement ( approximately 10(17)) of the reaction catalyzed by orotidine 5'-monophosphate decarboxylase (OMPDC) have not been defined. Catalysis requires a conformational change that closes an active site loop and "clamps" the orotate base proximal to hyd...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi100443a
更新日期：2010-05-04 00:00:00
abstract：:NG-Methyl-L-arginine (L-NMA) is one of the most commonly used inhibitors of the nitric oxide synthases (NOS). Results reported here demonstrate that L-NMA is an alternate substrate and a mechanism-based inhibitor of the inducible NOS purified from murine macrophages. The irreversible inhibition displays pseudo-first-o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00088a020
更新日期：1993-09-21 00:00:00
abstract：:The substrate analogue 2-(1-carboxy-2-phenylethyl)-4-phenylazophenol is a potent competitive inhibitor of carboxypeptidase A. Upon ligation to the active site, the azophenol moiety undergoes a shift of pKa from a value of 8.76 to a value of 4.9; this provides an index of the Lewis acidity of the active site zinc ion. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00358a028
更新日期：1986-05-20 00:00:00
abstract：:The NADH absorbance spectrum of nicotinoprotein (NADH-containing) alcohol dehydrogenase from Amycolatopsis methanolica has a maximum at 326 nm. Reduced enzyme-bound pyridine dinucleotide could be reversibly oxidized by acetaldehyde. The fluorescence excitation spectrum for NADH bound to the enzyme has a maximum at 325...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972115u
更新日期：1998-03-03 00:00:00
abstract：:Asymmetrically modified hemoglobins, [alpha(des-Arg)beta]A[alpha beta]cXL, [alpha(des-Arg-Tyr)beta]A[alpha beta]cXL, [alpha(des-Arg)beta(NES)]A[alpha beta]cXL, and [alpha(des-Arg)beta]A[alpha beta(NES)]cXL, have been prepared from chemically modified human normal adult hemoglobin (Hb A) and mutant hemoglobin C (beta 6...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00306a027
更新日期：1984-05-22 00:00:00
abstract：:Most duplex DNAs that are in the "B" conformation are not immunogenic. One important exception is poly(dG) X poly(dC), which produces a good immune response even though, by many criteria, it adopts a conventional right-handed helix. In order to investigate what features are being recognized, monoclonal antibodies were...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00309a024
更新日期：1984-07-03 00:00:00
abstract：:Insulin is very prone to form amyloid fibrils under slightly destabilizing conditions, and the B-chain region plays a critical role in the fibrillation. We show here that the isolated B-chain peptide of bovine insulin also forms fibrils at both acidic and neutral pH. When a mixture of insulin and the B-chain peptide w...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051658y
更新日期：2005-12-20 00:00:00
abstract：:Inhibitors of poly(ADP-ribose) polymerase (PARP, EC 2.4.2.30) are of clinical interest because they have potential for improving radiation therapy and chemotherapy of cancer. The refined binding structures of four such inhibitors are reported together with the refined structure of the unligated catalytic fragment of t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972383s
更新日期：1998-03-17 00:00:00
