Abstract：
:NG-Methyl-L-arginine (L-NMA) is one of the most commonly used inhibitors of the nitric oxide synthases (NOS). Results reported here demonstrate that L-NMA is an alternate substrate and a mechanism-based inhibitor of the inducible NOS purified from murine macrophages. The irreversible inhibition displays pseudo-first-order kinetics of inactivation with kinact = 0.07 min-1 and KI = 2.7 microM. Inactivation of NOS is enantiospecific for L-NMA, and substrate protection against inactivation is enantiospecific for L-arginine. L-NMA is hydroxylated, producing NG-hydroxy-NG-methyl-L-arginine (L-NHMA), and both compounds are slow, partially uncoupled alternate substrates for NOS. Processing of L-NMA by NOS results in four amino acid products: L-NHMA, NG-hydroxy-L-arginine (L-NHA), L-arginine, and citrulline. Deformylation of L-NMA and L-NHMA precedes the formation of citrulline and nitric oxide (.NO). Partial uncoupling of NADPH oxidation during L-NMA and L-NHMA processing results in hydrogen peroxide formation. The apparent Km values for L-NMA and L-NHMA are 3.1 and 7.4 microM, respectively. Turnover of L-NMA and L-NHMA to .NO and citrulline is slow relative to L-arginine: Vmax(L-arginine/L-NMA) = 20:1; Vmax(L-arginine)/(L-NHMA) = 13:1. NOS contains a functional cytochrome P-450-type heme, and the formation of these products from L-NMA is consistent with cytochrome P-450 monooxygenase chemistry. Other than the NOS reaction intermediate L-NHA, L-NMA and L-NHMA are the first NG-substituted L-arginines identified as substrates for NOS.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Olken NM,Marletta MAdoi
10.1021/bi00088a020subject
Has Abstractpub_date
1993-09-21 00:00:00pages
9677-85issue
37eissn
0006-2960issn
1520-4995journal_volume
32pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The synthetic peptide SNX-111 corresponding to the sequence of the omega-conopeptide MVIIA from the venom of the marine snail Conus magus is a highly potent and selective antagonist of N-type calcium channels. We have synthesized and characterized a large number of analogs of SNX-111 in order to elucidate the structur...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00025a013
更新日期：1995-06-27 00:00:00
abstract：:The rates of inactivation of BF1 adenosinetriphosphatase (BF1-ATPase) from Escherichia coli by 7-chloro-4-nitro-2,1,3-benzoxadiazole, 1-fluoro-2,4-dinitrobenzene, 2,4,6-trinitrobenzenesulfonate, phenylglyoxal, and N,N'-dicyclohexylcarbodiimide have been measured in the presence and absence of various concentrations of...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00531a011
更新日期：1982-01-19 00:00:00
abstract：:Cytochrome P450 3A4 is a drug-metabolizing enzyme of extraordinarily broad substrate specificity. This quality imparts upon the enzyme special importance in understanding its determinants of activity and substrate recognition. Limited successes in P450 3A4 active-site structure studies have been achieved by use of mec...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049231z
更新日期：2005-02-15 00:00:00
abstract：:Over 155 mutations within the V2 vasopressin receptor (AVPR2) gene are responsible for nephrogenic diabetes insipidus (NDI). The expression and subcellular distribution of four of these was investigated in transfected cells. These include a point mutation in the seventh transmembrane domain (S315R), a frameshift mutat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002699r
更新日期：2001-06-12 00:00:00
abstract：:The contributions to thermostability of interactions within the beta-sheet region of rubredoxins (Rds) were investigated by examining proteins in which beta-strand sequences of Rds from the hyperthermophilic archaeon Pyrococcus furiosus (Pf) and the mesophilic bacterium Clostridium pasteurianum (Cp) were interchanged....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970110r
更新日期：1997-08-26 00:00:00
abstract：:The major carbohydrate fragment from the lipophosphoglycan of Leishmania donovani was generated by mild acid hydrolysis (0.02 N HCl, 5 min, 100 degrees C) and purified by chromatography on DE-52 cellulose and thin layer. By a combination of analyses including gas-liquid chromatography-mass spectrometry and 1H NMR, the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00393a042
更新日期：1987-09-22 00:00:00
abstract：:Staphylococcus aureus transcription factor QacR regulates expression of the qacA multidrug efflux determinant. In response to binding cationic lipophilic compounds, including ethidium and rhodamine 6G, QacR dissociates from the qacA operator alleviating repression. Such ligand binding uniformly induces a coil-to-helix...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901102h
更新日期：2009-10-20 00:00:00
abstract：:Four different temperature-sensitive M protein mutants (tsM) of vesicular stomatitis virus (VSV) were characterized with regard to the association of the mutated M protein either with nucleocapsids or with membranes in the intact virions. Virions were labeled with the photoreactive hydrophobic probe [125I]iodonaphthyl...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00527a020
更新日期：1981-11-24 00:00:00
abstract：:The flap endonuclease, FEN1, plays a critical role in DNA replication and repair. Human FEN1 exhibits both a 5' to 3' exonucleolytic and a structure-specific endonucleolytic activity. On primer-template substrates containing an unannealed 5'-tail, or flap structure, FEN1 employs a unique mechanism to cleave at the poi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi991321u
更新日期：1999-10-05 00:00:00
abstract：:Fluorescence-detected circular dichroism (FDCD) spectra are reported for ethidium in Escherichia coli cells and bound to E. coli DNA in vitro. FDCD bands are observed at 325 and 385 nm. These bands change amplitude as the ethidium to DNA ratio changes. Spectra are similar for in vivo and in vitro measurements. However...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00333a001
更新日期：1985-06-04 00:00:00
abstract：:The purified ATPase of sarcoplasmic reticulum was covalently labeled with N-iodoacetyl-N'-(5-sulfo-1-naphthyl)ethylenediamine (1,5-IAEDANS) or with iodoacetamidofluorescein (IAF). In reconstituted vesicles containing both types of ATPase molecules fluorescence energy transfer was observed from the IAEDANS (donor) to t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00626a003
更新日期：1977-04-05 00:00:00
abstract：:Myelin basic protein (MBP) binds to negatively charged lipids on the cytosolic surface of oligodendrocyte membranes and is most likely responsible for adhesion of these surfaces in the multilayered myelin sheath. It can also polymerize actin, bundle F-actin filaments, and bind actin filaments to lipid bilayers through...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0519194
更新日期：2006-01-17 00:00:00
abstract：:Dihydrofolate reductase (DHFR) is often used as a model system to study the relation between protein dynamics and catalysis. We have studied a number of variants of the cold-adapted DHFR from Moritella profunda (MpDHFR), in which the catalytically important M20 and FG loops have been altered, and present a comparison ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi500508z
更新日期：2014-07-29 00:00:00
abstract：:The single-stranded packaged genome (ssDNA) of bacteriophage phi X174 is shown by Raman spectroscopy to lack both the ordered phosphodiester backbone and base stacking, which are demonstrated for unpackaged, protein-free ssDNA. In solutions of moderate ionic strength, unpackaged ssDNA contains 36 +/- 7% of deoxyribosy...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00234a004
更新日期：1991-05-21 00:00:00
abstract：:The role of basic residues of the 70-80-loop, Arg(74), Arg(75), and Lys(78) (chymotrypsin numbering) in the catalytic function of activated protein C (APC) was investigated by expressing mutants of protein C in which these residues were replaced with Ala in three separate constructs. Following purification to homogene...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi015899r
更新日期：2002-05-14 00:00:00
abstract：:Bovine hoof keratin was shown to be a substrate for cAMP-dependent protein kinase using [gamma-32P]ATP. Natural-abundance cross-polarization (CP) MAS 13C NMR was used to examine the effect of phosphorylation on keratin structure. When short contact times were used, phosphorylation was shown to increase the number of r...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00458a023
更新日期：1990-02-13 00:00:00
abstract：:The effects of pH on the kinetics of association and dissociation of chymotrypsin and the dipeptidyl trifluoromethyl ketone (TFK) N-acetyl-L-leucyl-L-phenylalanyltrifluoromethane (1) were examined through the pH range 4-9.5. The pH dependence of the association rate (kon) is similar to that of kcat/Km for ester and pe...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00449a017
更新日期：1989-11-14 00:00:00
abstract：:L-Aspartate oxidase (Laspo) catalyzes the conversion of L-Asp to iminoaspartate, the first step in the de novo biosynthesis of NAD(+). This bacterial pathway represents a potential drug target since it is absent in mammals. The Laspo R386L mutant was crystallized in the FAD-bound catalytically competent form and its t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi015939r
更新日期：2002-03-05 00:00:00
abstract：:The conformation and activity of three different lipases have been studied in reverse micelles formed by sodium bis(2-ethylhexyl) sulfosuccinate (AOT) in isooctane. In the case of human pancreatic lipase, the conformation of the polypeptide chain--as judged from far-UV circular dichroism measurements--is only slightly...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00066a025
更新日期：1993-04-20 00:00:00
abstract：:The 5'-untranslated region of positive-strand RNA viruses harbors many cis-acting RNA structural elements that are important for various viral processes such as replication, translation, and packaging of new virions. Among these is loop B RNA of the stem-loop IV domain within the internal ribosomal entry site (IRES) o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0363228
更新日期：2004-05-18 00:00:00
abstract：:Deuterium nuclear magnetic resonance spectra of dimyristoylphosphatidylcholines specifically labeled in positions 2', 3', 4', 6', 8', 10', 12', and 14', of the 2 chain, of an N-deuteriomethylphosphatidylcholine, and of cholesterol-3alpha-d1, have been obtained by the Fourier transform method at 5.46 and 3.52 T on two ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00607a006
更新日期：1978-07-11 00:00:00
abstract：:Brd4 belongs to the BET family. It is a multifunctional protein involved in transcription, replication, the signal transduction pathway, and cell cycle progression. All of these functions are linked to its association with acetylated chromatin. With its tandem bromodomains, Brd4 avidly binds to diacetylated H4-AcK5/K1...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi8001659
更新日期：2008-06-17 00:00:00
abstract：:A full-length cDNA encoding a calcium-dependent protein kinase with a calmodulin-like domain from Arabidopsis thaliana (AK-1 for Arabidopsis kinase-1) has been expressed as a fusion protein (called AK-1-6H) in Escherichia coli and purified to near homogeneity with high specific activity (typically 2000 nmol min-1 mg-1...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00174a008
更新日期：1994-03-01 00:00:00
abstract：:HIV-1 Vif is an accessory protein that induces the proteasomal degradation of the host restriction factor, apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3G (APOBEC3G). The N-terminal half of Vif binds to APOBEC3G, and the C-terminal half binds to subunits of a cullin 5-based ubiquitin ligase. This Vi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi201738a
更新日期：2012-03-13 00:00:00
abstract：:Atomic resolution ( journal_title：Biochemistry pub_type： 杂志文章 doi：10.1021/bi0517133 更新日期：2006-02-21 00:00:00
abstract：:The reverse transcriptase (RT) from the human immunodeficiency virus (HIV) is initially expressed as a 66-kDa protein and is subsequently proteolytically processed in vivo to form a 66-kDa/51-kDa heterodimer. Comparison of circular dichroism spectra of the 66-kDa, 51-kDa, and heterodimeric forms of RT indicates that t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00150a015
更新日期：1992-09-08 00:00:00
abstract：:Substitution of cysteine for proline-39 in Escherichia coli dihydrofolate reductase by oligonucleotide-directed mutagenesis positions the new cysteine adjacent to already existing cysteine-85. When the mutant protein is expressed in the E. coli cytosol, the cysteine sulfur atoms are found, by X-ray crystallographic an...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00382a017
更新日期：1987-04-21 00:00:00
abstract：:Bacterial nucleoid-associated proteins (NAPs) are critical to genome integrity and chromosome maintenance. Post-translational modifications of bacterial NAPs appear to function similarly to their better studied mammalian counterparts. The histone-like NAP HupB from Mycobacterium tuberculosis (Mtb) was previously obser...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b01089
更新日期：2018-02-06 00:00:00
abstract：:Guanylin, an intestinal peptide hormone and endogenous ligand of guanylyl cyclase C, is produced as the corresponding prohormone proguanylin. The mature hormone consists of 15 amino acid residues, representing the COOH-terminal part of the prohormone comprised of 94 amino acid residues. Here we report the recombinant ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi026434j
更新日期：2002-12-10 00:00:00
abstract：:Elucidation of the molecular mechanism leading to biomembrane fusion is a challenging issue in current biomedical research in view of its involvement in controlling cellular functions and in mediating various important diseases. According to the generally admitted stalk mechanism described for membrane fusion, negativ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060317x
更新日期：2006-08-01 00:00:00
