Characterization of annexin A1 glycan binding reveals binding to highly sulfated glycans with preference for highly sulfated heparan sulfate and heparin.

abstract：:The introduction into peptide chains of alpha-aminoisobutyric acid (Aib) has proven to stabilize the helical structure in short peptides by restricting the available range of polypeptide backbone conformations. In order to evaluate the potential stabilizing effect of Aib at the protein level, we have studied the confo...

journal_title：Biochemistry

authors： De Filippis V,De Antoni F,Frigo M,Polverino de Laureto P,Fontana A

更新日期：1998-02-10 00:00:00

abstract：:Phosphoenolpyruvate carboxykinase (PEPCK) is an essential metabolic enzyme operating in the gluconeogenesis and glyceroneogenesis pathways. Previous work has demonstrated that the enzyme cycles between a catalytically inactive open state and a catalytically active closed state. The transition of the enzyme between the...

journal_title：Biochemistry

authors： Johnson TA,Mcleod MJ,Holyoak T

更新日期：2016-01-26 00:00:00

abstract：:DREAM (also known as K(+) channel interacting protein 3 and calsenilin) is a calcium binding protein and an active modulator of KV4 channels in neuronal cells as well as a novel Ca(2+)-regulated transcriptional modulator. DREAM has also been associated with the regulation of Alzheimer's disease through the prevention ...

journal_title：Biochemistry

authors： Gonzalez WG,Ramos V,Diaz M,Garabedian A,Molano-Arevalo JC,Fernandez-Lima F,Miksovska J

更新日期：2016-03-29 00:00:00

abstract：:We compared the kinetics and thermodynamics of rhodamine phalloidin binding to actin purified from rabbit skeletal muscle, Acanthamoeba castellanii, and Saccharomyces cerevisiae in 50 mM KCl, 1 mM MgCl2, and pH 7.0 buffer at 22 degrees C. Filaments of S. cerevisiae actin bind rhodamine phalloidin more weakly than Acan...

journal_title：Biochemistry

authors： De La Cruz EM,Pollard TD

更新日期：1996-11-12 00:00:00

abstract：:Crystallographic studies of ligand-protein complexes reveal most preferable ligand binding modes, but do not show less populated modes that may contribute to measurable biochemical and biophysical characteristics of the complexes. In some cases, a ligand may bind a protein in essentially different modes. An example is...

journal_title：Biochemistry

authors： Blanchet J,Lin SX,Zhorov BS

更新日期：2005-05-17 00:00:00

abstract：:Electron spin echo envelope modulation (ESEEM) spectroscopy has been applied to the determination of the number of water molecules coordinated to the metal in the binary complex of staphylococcal nuclease with Mn2+, to the ternary enzyme-Mn2+-3',5'-pdTp complex, and to ternary complexes of a number of mutant enzymes i...

journal_title：Biochemistry

authors： Serpersu EH,McCracken J,Peisach J,Mildvan AS

更新日期：1988-10-18 00:00:00

abstract：:Pantothenate synthetase (PS, EC 6.3.2.1) is the last enzyme in the pantothenate biosynthesis pathway, a metabolic pathway identified as a potential target for new antimicrobials. PS catalyzes the ATP-dependent condensation of pantoate and beta-alanine to form pantothenate. Here we report the overexpression, purificati...

journal_title：Biochemistry

authors： Satoh A,Konishi S,Tamura H,Stickland HG,Whitney HM,Smith AG,Matsumura H,Inoue T

更新日期：2010-08-03 00:00:00

abstract：:A preparation consisting of isolated dimeric peripheral antenna complexes from green plant photosystem I (light-harvesting complex I or LHCI) has been characterized by means of (polarized) steady-state absorption and fluorescence spectroscopy at low temperatures. We show that this preparation can be described reasonab...

journal_title：Biochemistry

authors： Ihalainen JA,Gobets B,Sznee K,Brazzoli M,Croce R,Bassi R,van Grondelle R,Korppi-Tommola JE,Dekker JP

更新日期：2000-07-25 00:00:00

abstract：:The traditional approach to studying protein folding involves applying a perturbation, usually denaturant or mutation, and determining the effect upon the free energy of folding, DeltaG0, and the activation free energy, DeltaG(not equal). Data collected as a function of the perturbation can be used to construct rate e...

journal_title：Biochemistry

authors： Song B,Cho JH,Raleigh DP

更新日期：2007-12-11 00:00:00

abstract：:Histone deacetylases have proven to be attractive novel targets for the treatment of cancer. The first inhibitor of histone deacetylases was approved for the treatment of cutaneous T-cell lymphoma in 2006. The identification of new lead structures with improved effectiveness and fewer side effects is necessary. This r...

journal_title：Biochemistry

authors： Sykora J,Meyer-Almes FJ

更新日期：2010-02-23 00:00:00

abstract：:Utilizing structure-based design, we have previously demonstrated that it is possible to obtain selective inhibitors of protein-tyrosine phosphatase 1B (PTP1B). A basic nitrogen was introduced into a general PTP inhibitor to form a salt bridge to Asp48 in PTP1B and simultaneously cause repulsion in PTPs containing an ...

journal_title：Biochemistry

authors： Iversen LF,Andersen HS,Møller KB,Olsen OH,Peters GH,Branner S,Mortensen SB,Hansen TK,Lau J,Ge Y,Holsworth DD,Newman MJ,Hundahl Møller NP

更新日期：2001-12-11 00:00:00

abstract：:The calcitonin/CGRP gene pre-mRNA is alternatively spliced in a tissue-specific manner resulting in the formation of calcitonin mRNA in thyroid C-cells and CGRP mRNA in neurons. Computer analysis of the RNA containing the 3' splice acceptor of the calcitonin-specific exon 4 predicts that this region has the potential ...

journal_title：Biochemistry

authors： Coleman TP,Roesser JR

更新日期：1998-11-10 00:00:00

abstract：:Aminoacylation of a transfer RNA (tRNA) by its cognate aminoacyl-tRNA synthetase relies upon the recognition of specific nucleotides as well as conformational features within the tRNA by the synthetase. In Escherichia coli, the aminoacylation of tRNA(His) by histidyl-tRNA synthetase (HisRS) is highly dependent upon th...

journal_title：Biochemistry

authors： Connolly SA,Rosen AE,Musier-Forsyth K,Francklyn CS

更新日期：2004-02-03 00:00:00

abstract：:Hormone-sensitive lipase (HSL) is a rate-limiting enzyme in lipolysis that displays broad substrate specificity. HSL function is regulated by reversible phosphorylation that occurs within a 150 aa "regulatory module" of the protein. The current studies used mutational analysis to dissect the contribution of the "regul...

journal_title：Biochemistry

authors： Wang J,Shen WJ,Patel S,Harada K,Kraemer FB

更新日期：2005-02-15 00:00:00

abstract：:For some hormone receptors, the early events of signal transduction depend on their molecular arrangement and interactions at the cell surface. An understanding of the mechanism of signal transduction in general needs a careful analysis of the receptor distribution. Here, we present the first quantitative measurement ...

journal_title：Biochemistry

authors： St-Pierre PR,Petersen NO

更新日期：1992-03-10 00:00:00

abstract：:Di-leucine motifs have been implicated in the internalization or degradation of many membrane proteins. The epidermal growth factor receptor (EGFR) contains two di-leucine residues at 658 (TLRRLLQER) and 679 (NQALLRIL). To determine the role of these di-leucine motifs in regulating EGF receptor expression, activity, o...

journal_title：Biochemistry

authors： Morrison P,Chung KC,Rosner MR

更新日期：1996-11-19 00:00:00

abstract：:Fluorescence recovery after photobleaching was used to measure the diffusion coefficient of green fluorescent protein (GFP, 27 kDa) in Escherichia coli in the presence or absence of four coexpressed proteins: cytoplasmic maltose binding protein (42 kDa), tau-40 (45 kDa), alpha-synuclein (14 kDa), or calmodulin (17 kDa...

journal_title：Biochemistry

authors： Slade KM,Baker R,Chua M,Thompson NL,Pielak GJ

更新日期：2009-06-16 00:00:00

abstract：:In globular proteins, there are intermolecular hydrogen bonds between protein and water molecules, and between water molecules, which are bound with the proteins, in addition to intramolecular hydrogen bonds. To estimate the contribution of these hydrogen bonds to the conformational stability of a protein, the thermod...

journal_title：Biochemistry

authors： Takano K,Yamagata Y,Funahashi J,Hioki Y,Kuramitsu S,Yutani K

更新日期：1999-09-28 00:00:00

abstract：:We have investigated the role of the labile terminal domains of the core histones on the stability of the subunits of the protein core of the nucleosome by studying the thermodynamic behavior of the products of limited trypsin digestion of these subunits. The thermal stabilities of the truncated H2A-H2B dimer and the ...

journal_title：Biochemistry

authors： Karantza V,Freire E,Moudrianakis EN

更新日期：2001-10-30 00:00:00

abstract：:Salmonella typhimurium L-histidinol dehydrogenase (EC 1.1.1.23), a four-electron dehydrogenase, was inactivated by an active-site-directed modification reagent, 7-chloro-4-nitro-2,1,3-benzoxadiazole (NBD-Cl). The inactivation followed pseudo-first-order kinetics and was prevented by low concentrations of the substrate...

journal_title：Biochemistry

authors： Grubmeyer CT,Gray WR

更新日期：1986-08-26 00:00:00

abstract：:Factor IX activation by factor XIa is thought to proceed through the singly-cleaved free intermediate, factor IX alpha. However, we observed no intermediate development during factor IX activation by factor XIa when using a low substrate to enzyme ratio (44:1 mol/mol). This result can be explained by one of two mechan...

journal_title：Biochemistry

authors： Wolberg AS,Morris DP,Stafford DW

更新日期：1997-04-08 00:00:00

abstract：:Sorcin, a 21.6 kDa cytosolic EF-hand protein which undergoes a Ca(2+)-induced translocation from cytoplasm to membranes, has been assigned to the newly defined penta EF-hand family. A molecular model of the C-terminal Ca(2+)-binding domain has been generated using as a template the X-ray coordinates of the correspondi...

journal_title：Biochemistry

authors： Zamparelli C,Ilari A,Verzili D,Giangiacomo L,Colotti G,Pascarella S,Chiancone E

更新日期：2000-02-01 00:00:00

abstract：:Methylmalonyl-CoA mutase from Propionibacterium shermanii is an adenosylcobalamin-dependent enzyme which catalyzes the reversible isomerization of methylmalonyl-CoA and succinyl-CoA. The rate of tritium loss from 5'-[3H]adenosylcobalamin during the enzymic reaction and the relative rates of tritium appearance in subst...

journal_title：Biochemistry

authors： Meier TW,Thomä NH,Leadlay PF

更新日期：1996-09-10 00:00:00

abstract：:Although Mg(2+) is the cation that functions as the cofactor for the nucleotidyl transfer reaction for almost all DNA polymerases, Mn(2+) can also serve, but when it does, the degree of base discrimination exhibited by most DNA polymerases (pols) is diminished. Metal ions other than Mg(2+) or Mn(2+) can also act as co...

journal_title：Biochemistry

authors： Vashishtha AK,Konigsberg WH

更新日期：2016-05-10 00:00:00

abstract：:Two hybridomas producing monoclonal antibodies to poly(adenosine diphosphate ribose) [poly(ADP-Rib)] were established. One antibody, 10H (IgG3, kappa), bound to most of the poly(ADP-Rib) preparation, which consisted of molecules of various sizes of more than 20 ADP-Rib residues. The binding of this antibody was inhibi...

journal_title：Biochemistry

authors： Kawamitsu H,Hoshino H,Okada H,Miwa M,Momoi H,Sugimura T

更新日期：1984-07-31 00:00:00

abstract：:Heme uptake and utilization by pathogenic bacteria are critical for virulence and disease, since heme and heme proteins are a major source of iron within the host. Although the role of outer membrane heme receptors in this process has been extensively characterized at the genetic and biochemical level, the role of the...

journal_title：Biochemistry

authors： Kaur AP,Wilks A

更新日期：2007-03-20 00:00:00

abstract：:The structure of human epidermal growth factor (EGF, 53 amino acids) comprises three distinct loops (A, B, and C) connected correspondingly by the three native disulfide bonds, Cys(6)-Cys(20), Cys(14)-Cys(31), and Cys(33)-Cys(42). The connection of Cys(6) and Cys(20) forming the N-terminal A loop is essential for the ...

journal_title：Biochemistry

authors： Lu BY,Jiang C,Chang JY

更新日期：2005-11-15 00:00:00

abstract：:The rate of the reaction between p-nitroperoxybenzoic acid and cytochrome c peroxidase (CcP) has been investigated as a function of pH and ionic strength. The pH dependence of the reaction between CcP and peracetic acid has also been determined. The rate of the reactions are influenced by two heme-linked ionizations i...

journal_title：Biochemistry

authors： Palamakumbura AH,Foshay MC,Vitello LB,Erman JE

更新日期：1999-11-23 00:00:00

abstract：:The subtilisins have an extended substrate binding cleft comprising at least 8 subsites. Two pockets at the S1 and S4 sites are particularly conspicuous, and the interactions between substrate and these two pockets are very important for the substrate specificity. Phe residues have mutationally been introduced at one ...

journal_title：Biochemistry

authors： Bech LM,Sørensen SB,Breddam K

更新日期：1993-03-23 00:00:00

abstract：:The cAMP-dependent protein kinase (PKA) controls a large number of cellular functions. One critical PKA substrate in the brain and heart is the L-type Ca(2+) channel Ca(v)1.2, the activity of which is upregulated by PKA. The main PKA phosphorylation site is serine 1928 in the central pore forming alpha(1)1.2 subunit o...

journal_title：Biochemistry

authors： Hall DD,Feekes JA,Arachchige Don AS,Shi M,Hamid J,Chen L,Strack S,Zamponi GW,Horne MC,Hell JW

更新日期：2006-03-14 00:00:00