Characterization of annexin A1 glycan binding reveals binding to highly sulfated glycans with preference for highly sulfated heparan sulfate and heparin.


:Annexin A1 is a multifunctional, calcium-dependent phospholipid binding protein involved in a host of processes including inflammation, regulation of neuroendocrine signaling, apoptosis, and membrane trafficking. Binding of annexin A1 to glycans has been implicated in cell attachment and modulation of annexin A1 function. A detailed characterization of the glycan binding preferences of annexin A1 using carbohydrate microarrays and surface plasmon resonance served as a starting point to understand the role of glycan binding in annexin A1 function. Glycan array analysis identified annexin A1 binding to a series of sulfated oligosaccharides and revealed for the first time that annexin A1 binds to sulfated non-glycosaminoglycan carbohydrates. Using heparin/heparan sulfate microarrays, highly sulfated heparan sulfate/heparin were identified as preferred ligands of annexin A1. Binding of annexin A1 to heparin/heparan sulfate is calcium- but not magnesium-dependent. An in-depth structure-activity relationship of annexin A1-heparan sulfate interactions was established using chemically defined sugars. For the first time, a calcium-dependent heparin binding protein was characterized with such an approach. N-Sulfation and 2-O-sulfation were identified as particularly important for binding.






Horlacher T,Noti C,de Paz JL,Bindschädler P,Hecht ML,Smith DF,Fukuda MN,Seeberger PH




Has Abstract


2011-04-05 00:00:00












  • Association of insulin-like growth factor 2 with the insulin-linked polymorphic region in cultured fetal thymus cells.

    abstract::The insulin-linked polymorphic region (ILPR) is a regulatory sequence in the promoter region upstream of the human insulin gene and is widely recognized as a locus of type 1 diabetes susceptibility. Polymorphism of the ILPR sequence can affect expression of both insulin and the adjacent insulin-like growth factor 2 (I...


    pub_type: 杂志文章


    authors: Wang Y,Zhang H,Ligon LA,McGown LB

    更新日期:2009-09-01 00:00:00

  • Human myeloperoxidase: structure of a cyanide complex and its interaction with bromide and thiocyanate substrates at 1.9 A resolution.

    abstract::The 1.9 A X-ray crystal structure of human myeloperoxidase complexed with cyanide (R = 0.175, R(free) = 0.215) indicates that cyanide binds to the heme iron with a bent Fe-C-N angle of approximately 157 degrees, and binding is accompanied by movement of the iron atom by 0.2 A into the porphyrin plane. The bent orienta...


    pub_type: 杂志文章


    authors: Blair-Johnson M,Fiedler T,Fenna R

    更新日期:2001-11-20 00:00:00

  • An internal cysteine plays a role in the maintenance of the latency of human fibroblast collagenase.

    abstract::The cDNA that encodes the proenzyme form of human fibroblast collagenase has been expressed in Escherichia coli. It has been shown by a number of criteria to be functionally identical with the enzyme isolated from human sources. Mutations of each of three cysteine residues found in procollagenase were constructed by s...


    pub_type: 杂志文章


    authors: Windsor LJ,Birkedal-Hansen H,Birkedal-Hansen B,Engler JA

    更新日期:1991-01-22 00:00:00

  • Carbon monoxide binding properties of hemoglobin M Iwate.

    abstract::The kinetic and equilibrium CO binding properties of hemoglobin (Hb) M Iwate (alpha2 87 His leads to Tyr beta2) have been investigated. The results show that the alpha(Met) beta2 (CO) tetramer of this protein has a low affinity for CO, as indicated by the stopped-flow and flash-photolysis kinetic, as well as the CO bi...


    pub_type: 杂志文章


    authors: Salhany JM,Castillo CL,Ogawa S

    更新日期:1976-11-30 00:00:00

  • Metal-selective DNA-binding response of Escherichia coli NikR.

    abstract::The NikR transcription factor from Escherichia coli is a Ni(II)-dependent repressor that regulates production of the nickel ion transporter encoded by the nik operon. In the previous paper in this issue (Wang, S. C., Dias, A., Bloom, S. L., and Zamble, D. B. (2004) Selectivity of Metal Binding and Metal-Induced Stabil...


    pub_type: 杂志文章


    authors: Bloom SL,Zamble DB

    更新日期:2004-08-10 00:00:00

  • Main chain and side chain dynamics of a heme protein: 15N and 2H NMR relaxation studies of R. capsulatus ferrocytochrome c2.

    abstract::A detailed characterization of the main chain and side chain dynamics in R. capsulatus ferrocytochrome c(2) derived from (2)H NMR relaxation of methyl group resonances is presented. (15)N relaxation measurements confirm earlier results indicating that R. capsulatus ferrocytochrome c(2) exhibits minor rotational anisot...


    pub_type: 杂志文章


    authors: Flynn PF,Bieber Urbauer RJ,Zhang H,Lee AL,Wand AJ

    更新日期:2001-06-05 00:00:00

  • Steric requirements at position B12 for high biological activity in insulin.

    abstract::The alpha-helix formed by the amino acid residues 9-19 of the B-chain of insulin is involved in the stabilization of its three-dimensional structure. We have shown that modification at positions B9, B10, B12, and B16 results in analogues possessing biological activities ranging from ca. 0.2% to ca. 500% relative to th...


    pub_type: 杂志文章


    authors: Hu SQ,Burke GT,Schwartz GP,Ferderigos N,Ross JB,Katsoyannis PG

    更新日期:1993-03-16 00:00:00

  • Amino acid sequence of the nucleotide binding region of chloroplast coupling factor 1.

    abstract::The labeling of chloroplast coupling factor 1 by 3'-O-(4-benzoyl)benzoyl-ATP (BzATP) was studied. When the enzyme was incubated with approximately 10 microM BzATP and 6 mM MgCl2 at pH 7.9 for approximately 20 min and passed through two Sephadex G-50 centrifuge columns, three BzATP molecules were bound per coupling fac...


    pub_type: 杂志文章


    authors: Admon A,Hammes GG

    更新日期:1987-06-02 00:00:00

  • A novel pentasaccharide sequence GlcA(3-sulfate)(beta1-3)GalNAc(4-sulfate)(beta1-4)(Fuc alpha1-3)GlcA(beta1-3)GalNAc(4-sulfate) in the oligosaccharides isolated from king crab cartilage chondroitin sulfate K and its differential susceptibility to chondroi

    abstract::Novel sulfated tetrasaccharide structures containing 3-O-sulfated GlcA were isolated recently from king crab cartilage chondroitin sulfate K [Sugahara, K., Tanaka, Y., Yamada, S., Seno, N., Kitagawa, H., Haslam, S. M., Morris, H. R., & Dell, A. (1996) J. Biol. Chem. 271, 26745-26754]. In this study, we prepared a seri...


    pub_type: 杂志文章


    authors: Kitagawa H,Tanaka Y,Yamada S,Seno N,Haslam SM,Morris HR,Dell A,Sugahara K

    更新日期:1997-04-01 00:00:00

  • Order and dynamics in mixtures of membrane glucolipids from Acholeplasma laidlawii studied by 2H NMR.

    abstract::The two dominant glucolipids in Acholeplasma laidlawii, viz., 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol (MGlcDG) and 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1----2)-O-alpha-D-glucopyranosyl ]- sn-glycerol (DGlcDG), have markedly different phase behavior. MGlcDG has an ability to form nonlamellar phases, wher...


    pub_type: 杂志文章


    authors: Eriksson PO,Rilfors L,Wieslander A,Lundberg A,Lindblom G

    更新日期:1991-05-21 00:00:00

  • Inhibitors of the molybdenum cofactor containing 4-hydroxybenzoyl-CoA reductase.

    abstract::4-Hydroxybenzoyl-CoA reductase (4-HBCR) is a member of the xanthine oxidase (XO) family of molybdenum cofactor containing enzymes and catalyzes the irreversible removal of a phenolic hydroxy group by reduction, yielding benzoyl-CoA and water. In this work the effects of various activity modulating compounds were chara...


    pub_type: 杂志文章


    authors: Johannes J,Unciuleac MC,Friedrich T,Warkentin E,Ermler U,Boll M

    更新日期:2008-04-29 00:00:00

  • Determination of the essentiality of the eight cysteine residues of the NrtA protein for high-affinity nitrate transport and the generation of a functional cysteine-less transporter.

    abstract::All eight cysteine residues, C90, C94, C143, C147, C219, C325, C367, and C431, present in transmembrane domains of the Aspergillus nidulans NrtA nitrate transporter protein were altered individually by site-specific mutagenesis. The results indicate that six residues, C90, C147, C219, C325, C367, and C431, are not req...


    pub_type: 杂志文章


    authors: Unkles SE,Rouch DA,Wang Y,Siddiqi MY,Okamoto M,Stephenson RM,Kinghorn JR,Glass AD

    更新日期:2005-04-12 00:00:00

  • Extreme Expression of DNA Repair Protein Apurinic/Apyrimidinic Endonuclease 1 (APE1) in Human Breast Cancer As Measured by Liquid Chromatography and Isotope Dilution Tandem Mass Spectrometry.

    abstract::Apurinic/apyrimidinic endonuclease 1 (APE1) is a DNA repair protein and plays other important roles. Increased levels of APE1 in cancer have been reported. However, available methods for measuring APE1 levels are indirect and not quantitative. We previously developed an approach using liquid chromatography and tandem ...


    pub_type: 杂志文章


    authors: Coskun E,Jaruga P,Reddy PT,Dizdaroglu M

    更新日期:2015-09-29 00:00:00

  • Crystal structure of ribonuclease T1 carboxymethylated at Glu58 in complex with 2'-GMP.

    abstract::The carboxymethylation of RNase T1 at the gamma-carboxyl group of Glu58 leads to a complete loss of the enzymatic activity while it retains substrate-binding ability. Accompanying the carboxymethylation, RNase T1 undergoes a remarkable thermal stabilization of 9 degrees C in the melting temperature (Tm). In order to c...


    pub_type: 杂志文章


    authors: Ishikawa K,Suzuki E,Tanokura M,Takahashi K

    更新日期:1996-06-25 00:00:00

  • Structures of enterococcal glycerol kinase in the absence and presence of glycerol: correlation of conformation to substrate binding and a mechanism of activation by phosphorylation.

    abstract::The first structure of a glycerol kinase from a Gram-positive organism, Enterococcus casseliflavus, has been determined to 2.8 A resolution in the presence of glycerol and to 2.5 A resolution in the absence of substrate. The substrate-induced closure of 7 degrees is significantly smaller than that reported for hexokin...


    pub_type: 杂志文章


    authors: Yeh JI,Charrier V,Paulo J,Hou L,Darbon E,Claiborne A,Hol WG,Deutscher J

    更新日期:2004-01-20 00:00:00

  • Mechanism of human alpha-1,3-fucosyltransferase V: glycosidic cleavage occurs prior to nucleophilic attack.

    abstract::alpha-1,3-Fucosyltransferase V (FucT V) catalyzes the transfer of 1-fucose from the donor sugar guanosine 5'-diphospho-beta-1-fucose (GDP-Fuc) to an acceptor sugar. A secondary isotope effect on the fucosyltransfer reaction with guanosine 5'-diphospho-[1-2H]-beta-1-fucose (GDP-[1-2H]-Fuc) as the substrate was observed...


    pub_type: 杂志文章


    authors: Murray BW,Wittmann V,Burkart MD,Hung SC,Wong CH

    更新日期:1997-01-28 00:00:00

  • Metal-ligand vibrations of cyanoferric myeloperoxidase and cyanoferric horseradish peroxidase: evidence for a constrained heme pocket in myeloperoxidase.

    abstract::The low-frequency FeCN vibrations of cyanoferric myeloperoxidase (MPO) and horseradish peroxidase (HRP) have been measured by resonance Raman spectroscopy. The ordering of the frequencies of the predominantly FeC stretching and FeCN bending normal vibrational modes in the two peroxidases differs. These normal mode vib...


    pub_type: 杂志文章


    authors: López-Garriga JJ,Oertling WA,Kean RT,Hoogland H,Wever R,Babcock GT

    更新日期:1990-10-09 00:00:00

  • Enzymic hydrolysis of phosphonate esters. Reaction mechanism of intestinal 5'-nucleotide phosphodiesterase.

    abstract::The mechanism of bovine intestinal 5'-nucleotide phosphodiesterase was investigated by determining kinetic constants of systematically varied substrates, with emphasis on esters of phosphonic acids (which have much higer Vmax values than conventional phosphodiester substrates), and by pre-steady-state kinetics using b...


    pub_type: 杂志文章


    authors: Kelly SJ,Butler LG

    更新日期:1977-03-22 00:00:00

  • Protein arginine deiminase 4: evidence for a reverse protonation mechanism.

    abstract::The presumed role of an overactive protein arginine deiminase 4 (PAD4) in the pathophysiology of rheumatoid arthritis (RA) suggests that PAD4 inhibitors could be used to treat an underlying cause of RA, potentially offering a mechanism to stop further disease progression. Thus, the development of such inhibitors is of...


    pub_type: 杂志文章


    authors: Knuckley B,Bhatia M,Thompson PR

    更新日期:2007-06-05 00:00:00

  • Copper- and silver-substituted yeast metallothioneins: sequential 1H NMR assignments reflecting conformational heterogeneity at the C terminus.

    abstract::Complete 1H NMR sequential assignments have been made for copper(I)- and silver (I)-substituted metallothionein (MT) from Saccharomyces cerevisiae using standard 2D 1H NMR methods. The fingerprint region of the COSY spectrum of both metalloproteins shows a doubling of a few backbone proton resonances from residue K41 ...


    pub_type: 杂志文章


    authors: Narula SS,Winge DR,Armitage IM

    更新日期:1993-07-06 00:00:00

  • Evidence for a quantitative tissue-specific distribution of high mobility group chromosomal proteins.

    abstract::The quantitative tissue specificity of the high mobility group (HMG) chromosomal proteins was investigated. Perchloric acid (PCA) extracts of four different chicken tissues and erythrocytes contained three proteins which comigrated on NaDodSO4-polyacrylamide gels with the HMG's 1,2, and E from erythrocyte nuclei. Thes...


    pub_type: 杂志文章


    authors: Gordon JS,Rosenfeld BI,Kaufman R,Williams DL

    更新日期:1980-09-16 00:00:00

  • Dynamics and binding mode of Hoechst 33258 to d(GTGGAATTCCAC)2 in the 1:1 solution complex as determined by two-dimensional 1H NMR.

    abstract::We have investigated the interaction of the bisbenzimidazole derivative Hoechst 33258 with the self-complementary dodecadeoxynucleotide duplex d(GTGGAATTCCAC)2 using one-dimensional (1D) and two-dimensional (2D) proton nuclear magnetic resonance (1H NMR) spectroscopy. To monitor the extent of complex formation, we use...


    pub_type: 杂志文章


    authors: Fede A,Labhardt A,Bannwarth W,Leupin W

    更新日期:1991-12-03 00:00:00

  • Medium-Throughput Screen of Microbially Produced Serotonin via a G-Protein-Coupled Receptor-Based Sensor.

    abstract::Chemical biosensors, for which chemical detection triggers a fluorescent signal, have the potential to accelerate the screening of noncolorimetric chemicals produced by microbes, enabling the high-throughput engineering of enzymes and metabolic pathways. Here, we engineer a G-protein-coupled receptor (GPCR)-based sens...


    pub_type: 杂志文章


    authors: Ehrenworth AM,Claiborne T,Peralta-Yahya P

    更新日期:2017-10-17 00:00:00

  • Selective radiolabeling and isolation of the hydrophobic membrane-binding domain of human erythrocyte acetylcholinesterase.

    abstract::The hydrophobic, membrane-binding domain of purified human erythrocyte acetylcholinesterase was labeled with the photoactivated reagent 3-(trifluoromethyl)-3-(m-[125I]iodophenyl)diazirine. The radiolabel was incorporated when the enzyme was prepared in detergent-free aggregates, in detergent micelles, or in phospholip...


    pub_type: 杂志文章


    authors: Roberts WL,Rosenberry TL

    更新日期:1986-06-03 00:00:00

  • Structure of lithocholic acid binding to the N-terminal 8-kDa domain of DNA polymerase beta.

    abstract::The purpose of this study was to investigate the molecular action of lithocholic acid (LCA), known as a selective inhibitor of DNA polymerase beta (pol beta). The 39-kDa pol beta was separated proteolytically into two fragments of the template-primer binding domain (8 kDa) and the catalytic domain (31 kDa). LCA bound ...


    pub_type: 杂志文章


    authors: Mizushina Y,Ohkubo T,Sugawara F,Sakaguchi K

    更新日期:2000-10-17 00:00:00

  • Structural analysis of integrin recognition and the inhibition of integrin-mediated cell functions by novel nonpeptidic surrogates of the Arg-Gly-Asp sequence.

    abstract::The pivotal role of the Arg-Gly-Asp (RGD) peptide motif in integrin-mediated cell adhesive interactions with extracellular matrix and plasma proteins stimulated the present design of nonpeptidic mimetics of this sequence. To probe the structural requirements for RGD recognition by integrins, we designed various struct...


    pub_type: 杂志文章


    authors: Greenspoon N,Hershkoviz R,Alon R,Varon D,Shenkman B,Marx G,Federman S,Kapustina G,Lider O

    更新日期:1993-02-02 00:00:00

  • Assignment of disulfide bond location in prothoracicotropic hormone of the silkworm, Bombyx mori: a homodimeric peptide.

    abstract::The disulfide bond location of a homodimeric peptide, prothoracicotropic hormone (PTTH) of the silkworm, Bombyx mori, was determined by a combination of partial reduction and sequence analysis of peptide fragments generated through a partial reduction of PTTH followed by alkylation and enzyme digestion. The partial re...


    pub_type: 杂志文章


    authors: Ishibashi J,Kataoka H,Isogai A,Kawakami A,Saegusa H,Yagi Y,Mizoguchi A,Ishizaki H,Suzuki A

    更新日期:1994-05-17 00:00:00

  • Mechanistic insights from the binding of substrate and carbocation intermediate analogues to aristolochene synthase.

    abstract::Aristolochene synthase, a metal-dependent sesquiterpene cyclase from Aspergillus terreus, catalyzes the ionization-dependent cyclization of farnesyl diphosphate (FPP) to form the bicyclic eremophilane (+)-aristolochene with perfect structural and stereochemical precision. Here, we report the X-ray crystal structure of...


    pub_type: 杂志文章


    authors: Chen M,Al-lami N,Janvier M,D'Antonio EL,Faraldos JA,Cane DE,Allemann RK,Christianson DW

    更新日期:2013-08-13 00:00:00

  • Activity of one of two engineered heterodimers of AhpF, the NADH:peroxiredoxin oxidoreductase from Salmonella typhimurium, reveals intrasubunit electron transfer between domains.

    abstract::AhpF, the flavoprotein reductase component of the Salmonella typhimurium alkyl hydroperoxide reductase system, catalyzes the reduction of an intersubunit disulfide bond in the peroxidatic active site of the system's other component, AhpC, a member of the peroxiredoxin family. Previous studies have shown that AhpF can ...


    pub_type: 杂志文章


    authors: Reynolds CM,Poole LB

    更新日期:2001-04-03 00:00:00

  • cAMP binds with high affinity and specificity to the androgen receptor from murine skeletal muscle.

    abstract::cAMP binding of the androgen receptor (AR) from murine skeletal muscle was studied. Testosterone affinity chromatography yielded androgen receptor with about 4000-fold purification. Determination of the cAMP binding in the affinity eluate, by adsorption of protein-cAMP complexes to cellulose ester filters or removal o...


    pub_type: 杂志文章


    authors: Ofenloch-Hähnle B,Eisele K

    更新日期:1988-11-15 00:00:00