Characterization of annexin A1 glycan binding reveals binding to highly sulfated glycans with preference for highly sulfated heparan sulfate and heparin.


:Annexin A1 is a multifunctional, calcium-dependent phospholipid binding protein involved in a host of processes including inflammation, regulation of neuroendocrine signaling, apoptosis, and membrane trafficking. Binding of annexin A1 to glycans has been implicated in cell attachment and modulation of annexin A1 function. A detailed characterization of the glycan binding preferences of annexin A1 using carbohydrate microarrays and surface plasmon resonance served as a starting point to understand the role of glycan binding in annexin A1 function. Glycan array analysis identified annexin A1 binding to a series of sulfated oligosaccharides and revealed for the first time that annexin A1 binds to sulfated non-glycosaminoglycan carbohydrates. Using heparin/heparan sulfate microarrays, highly sulfated heparan sulfate/heparin were identified as preferred ligands of annexin A1. Binding of annexin A1 to heparin/heparan sulfate is calcium- but not magnesium-dependent. An in-depth structure-activity relationship of annexin A1-heparan sulfate interactions was established using chemically defined sugars. For the first time, a calcium-dependent heparin binding protein was characterized with such an approach. N-Sulfation and 2-O-sulfation were identified as particularly important for binding.






Horlacher T,Noti C,de Paz JL,Bindschädler P,Hecht ML,Smith DF,Fukuda MN,Seeberger PH




Has Abstract


2011-04-05 00:00:00












  • Bacterial ApbC can bind and effectively transfer iron-sulfur clusters.

    abstract::The metabolism of iron-sulfur ([Fe-S]) clusters requires a complex set of machinery that is still being defined. Mutants of Salmonella enterica lacking apbC have nutritional and biochemical properties indicative of defects in [Fe-S] cluster metabolism. ApbC is a 40.8 kDa homodimeric ATPase and as purified contains lit...


    pub_type: 杂志文章


    authors: Boyd JM,Pierik AJ,Netz DJ,Lill R,Downs DM

    更新日期:2008-08-05 00:00:00

  • Identification and mechanism of a bacterial hydrolyzing UDP-N-acetylglucosamine 2-epimerase.

    abstract::This paper reports the first identification of a fully functional hydrolyzing UDP-N-acetylglucosamine 2-epimerase from a bacterial source. The epimerase (known as SiaA or NeuC) from Neisseria meningitidis MC58 group B is shown to catalyze the conversion of UDP-GlcNAc into ManNAc and UDP in the first step of sialic aci...


    pub_type: 杂志文章


    authors: Murkin AS,Chou WK,Wakarchuk WW,Tanner ME

    更新日期:2004-11-09 00:00:00

  • Substrate specificity of the heparan sulfate hexuronic acid 2-O-sulfotransferase.

    abstract::The interaction of heparan sulfate with different ligand proteins depends on the precise location of O-sulfate groups in the polysaccharide chain. We have previously shown that overexpression in human kidney 293 cells of a mouse mastocytoma 2-O-sulfotransferase (2-OST), previously thought to catalyze the transfer of s...


    pub_type: 杂志文章


    authors: Rong J,Habuchi H,Kimata K,Lindahl U,Kusche-Gullberg M

    更新日期:2001-05-08 00:00:00

  • Structural, dynamic, and chemical characterization of a novel S-glycosylated bacteriocin.

    abstract::Bacteriocins are bacterial peptides with specific activity against competing species. They hold great potential as natural preservatives and for their probiotic effects. We show here nuclear magnetic resonance-based evidence that glycocin F, a 43-amino acid bacteriocin from Lactobacillus plantarum, contains two β-link...


    pub_type: 杂志文章


    authors: Venugopal H,Edwards PJ,Schwalbe M,Claridge JK,Libich DS,Stepper J,Loo T,Patchett ML,Norris GE,Pascal SM

    更新日期:2011-04-12 00:00:00

  • Mechanistic aspects of the DNA junction-resolving enzyme T7 endonuclease I.

    abstract::The chemical mechanism of phosphodiester bond hydrolysis catalyzed by a junction-resolving enzyme has been investigated. Endonuclease I of phage T7 is a member of the nuclease superfamily of proteins that include many restriction enzymes, and the structure of the active site is very similar to that of BglI in particul...


    pub_type: 杂志文章


    authors: Liu J,Déclais AC,Lilley DM

    更新日期:2006-03-28 00:00:00

  • Interaction between the Rev1 C-Terminal Domain and the PolD3 Subunit of Polζ Suggests a Mechanism of Polymerase Exchange upon Rev1/Polζ-Dependent Translesion Synthesis.

    abstract::Translesion synthesis (TLS) is a mutagenic branch of cellular DNA damage tolerance that enables bypass replication over DNA lesions carried out by specialized low-fidelity DNA polymerases. The replicative bypass of most types of DNA damage is performed in a two-step process of Rev1/Polζ-dependent TLS. In the first ste...


    pub_type: 杂志文章


    authors: Pustovalova Y,Magalhães MT,D'Souza S,Rizzo AA,Korza G,Walker GC,Korzhnev DM

    更新日期:2016-04-05 00:00:00

  • Chemistry of gene silencing: the mechanism of NAD+-dependent deacetylation reactions.

    abstract::The Sir2 enzyme family is responsible for a newly classified chemical reaction, NAD(+)-dependent protein deacetylation. New peptide substrates, the reaction mechanism, and the products of the acetyl transfer to NAD(+) are described for SIR2. The final products of SIR2 reactions are the deacetylated peptide and the 2' ...


    pub_type: 杂志文章


    authors: Sauve AA,Celic I,Avalos J,Deng H,Boeke JD,Schramm VL

    更新日期:2001-12-25 00:00:00

  • Investigation of the structure of bovine erythrocyte superoxide dismutase by 1H nuclear magnetic resonance spectroscopy.

    abstract::The 270-MHz 1H nuclear magnetic resonance spectra of the apo, copper(I)-zinc(II), and copper(II)-zinc(II) forms of bovine erythrocyte superoxide dismutase (EC are reported, and assignments of resonances to ten amino acid residues are proposed. The data require that at least four and probably six histidine re...


    pub_type: 杂志文章


    authors: Cass AE,Hill AO,Smith BE,Bannister JV,Bannister WH

    更新日期:1977-07-12 00:00:00

  • Measurement of DNA-protein equilibria using gel chromatography: application to the HinfI restriction endonuclease.

    abstract::A method is described for measuring equilibrium constants of DNA-protein interactions using gel chromatography. This technique has been used to study the sequence-specific interaction of the HinfI restriction endonuclease with DNA. HinfI has a monomeric molecular weight of 31000 and exists as a dimer in its active for...


    pub_type: 杂志文章


    authors: Frankel AD,Ackers GK,Smith HO

    更新日期:1985-06-04 00:00:00

  • 9-(5',6'-dideoxy-beta-D-ribo-hex-5'-ynofuranosyl)adenine, a novel irreversible inhibitor of S-adenosylhomocysteine hydrolase.

    abstract::The acetylenic analogue of adenosine 9-(5',6'-dideoxy-beta-D-ribo-hex-5'-ynofuranosyl)adenine has been synthesized, and its behavior as an inhibitor of bovine S-adenosylhomocysteine hydrolase has been examined. Incubation of the enzyme with excess inhibitor caused a time-dependent, irreversible inactivation of the enz...


    pub_type: 杂志文章


    authors: Parry RJ,Muscate A,Askonas LJ

    更新日期:1991-10-15 00:00:00

  • Evidence from base-pair kinetics for two types of adenine tract structures in solution: their relation to DNA curvature.

    abstract::We have measured the base-pair lifetimes in oligodeoxynucleotides containing tracts of A.T base pairs using imino proton magnetic resonance. When the tract contains more than four consecutive A.T base pairs, possibly including a 5'-AT step but not a 5'-TA step, anomalously long lifetimes are observed. For example, the...


    pub_type: 杂志文章


    authors: Leroy JL,Charretier E,Kochoyan M,Guéron M

    更新日期:1988-12-13 00:00:00

  • Neurotoxins active on insects: amino acid sequences, chemical modifications, and secondary structure estimation by circular dichroism of toxins from the scorpion Androctonus australis Hector.

    abstract::Two scorpion neurotoxins active only on insects, the insect toxins AaH IT1 and AaH IT2, were purified from the venom of scorpions Androctonus australis Hector collected in Tozeur (Tunisia) and characterized. AaH IT2 was sequenced and found to differ in four amino acid positions from AaH IT, the single previously seque...


    pub_type: 杂志文章


    authors: Loret EP,Mansuelle P,Rochat H,Granier C

    更新日期:1990-02-13 00:00:00

  • The N-terminus of apolipoprotein A-V adopts a helix bundle molecular architecture.

    abstract::Previous studies of recombinant full-length human apolipoprotein A-V (apoA-V) provided evidence of the presence of two independently folded structural domains. Computer-assisted sequence analysis and limited proteolysis studies identified an N-terminal fragment as a candidate for one of the domains. C-Terminal truncat...


    pub_type: 杂志文章


    authors: Wong K,Beckstead JA,Lee D,Weers PM,Guigard E,Kay CM,Ryan RO

    更新日期:2008-08-19 00:00:00

  • Backbone dynamics of the glucocorticoid receptor DNA-binding domain.

    abstract::The extent of rapid (picosecond) backbone motions within the glucocorticoid receptor DNA-binding domain (GR DBD) has been investigated using proton-detected heteronuclear NMR spectroscopy on uniformly 15N-labeled protein fragments containing the GR DBD. Sequence-specific 15N resonance assignments, based on two- and th...


    pub_type: 杂志文章


    authors: Berglund H,Kovács H,Dahlman-Wright K,Gustafsson JA,Härd T

    更新日期:1992-12-08 00:00:00

  • Conversion of aminodeoxychorismate synthase into anthranilate synthase with Janus mutations: mechanism of pyruvate elimination catalyzed by chorismate enzymes.

    abstract::The central importance of chorismate enzymes in bacteria, fungi, parasites, and plants combined with their absence in mammals makes them attractive targets for antimicrobials and herbicides. Two of these enzymes, anthranilate synthase (AS) and aminodeoxychorismate synthase (ADCS), are structurally and mechanistically ...


    pub_type: 杂志文章


    authors: Culbertson JE,Chung Dh,Ziebart KT,Espiritu E,Toney MD

    更新日期:2015-04-14 00:00:00

  • Manganese(II)-dependent extradiol-cleaving catechol dioxygenase from Arthrobacter globiformis CM-2.

    abstract::A manganese-dependent 3,4-dihydroxyphenylactate 2,3-dioxygenase from Arthrobacter globiformis strain CM-2 (MndD) cloned in Escherichia coli has been purified to homogeneity. Sedimentation equilibrium analysis indicates an alpha 4 homotetrameric holoenzyme structure (4 x 38,861 Da). Steady-state kinetic analysis of Mnd...


    pub_type: 杂志文章


    authors: Whiting AK,Boldt YR,Hendrich MP,Wackett LP,Que L Jr

    更新日期:1996-01-09 00:00:00

  • Structure-function relationships in human glutathione-dependent formaldehyde dehydrogenase. Role of Glu-67 and Arg-368 in the catalytic mechanism.

    abstract::The active-site zinc in human glutathione-dependent formaldehyde dehydrogenase (FDH) undergoes coenzyme-induced displacement and transient coordination to a highly conserved glutamate residue (Glu-67) during the catalytic cycle. The role of this transient coordination of the active-site zinc to Glu-67 in the FDH catal...


    pub_type: 杂志文章


    authors: Sanghani PC,Davis WI,Zhai L,Robinson H

    更新日期:2006-04-18 00:00:00

  • Multiple-step kinetic mechanisms of the ssDNA recognition process by human polymerase beta in its different ssDNA binding modes.

    abstract::The kinetics of human polymerase beta (pol beta) binding to the single-stranded DNA, in the (pol beta)(16) and (pol beta)(5) binding modes, that differ in the number of occluded nucleotide residues in the protein-DNA complexes, have been examined, using the fluorescence stopped-flow technique. This is the first determ...


    pub_type: 杂志文章


    authors: Rajendran S,Jezewska MJ,Bujalowski W

    更新日期:2001-10-02 00:00:00

  • Stepwise modulation of ATPase activity, nucleotide trapping, and sliding motility of myosin S1 by modification of the thiol region with residues of increasing size.

    abstract::Rabbit muscle myosin S1 was modified either at SH1 alone or at both SH1 and SH2, using a series of alkylthiolating reagents of increasing size, designed for correlating gradually changing structural disturbances in the thiol region with functional impairments in the myosin head. The reagents were of the type H(CH(2))(...


    pub_type: 杂志文章


    authors: Tiepold M,Kliche W,Pfannstiel J,Faulstich H

    更新日期:2000-02-15 00:00:00

  • Effects of hydration on purine motion in solid DNA.

    abstract::Deuterium quadrupole echo spectra and spin-lattice relaxation rates measured at 76.8 and 38.4 MHz as a function of relative humidity are reported for calf thymus DNA deuterated at positions A8 and G8. The amplitude of base pair motion is observed to increase slightly with increasing degree of hydration (up to approxim...


    pub_type: 杂志文章


    authors: Brandes R,Vold RR,Vold RL,Kearns DR

    更新日期:1986-11-18 00:00:00

  • Activators of phosphorylase kinase alter the cross-linking of its catalytic subunit to the C-terminal one-sixth of its regulatory alpha subunit.

    abstract::Phosphorylase kinase, a regulatory enzyme of glycogenolysis in skeletal muscle, is a hexadecameric oligomer consisting of four copies each of a catalytic subunit (gamma) and three regulatory subunits (alpha, beta, and delta, the last being endogenous calmodulin). The enzyme is activated by a variety of effectors actin...


    pub_type: 杂志文章


    authors: Nadeau OW,Traxler KW,Fee LR,Baldwin BA,Carlson GM

    更新日期:1999-02-23 00:00:00

  • Mapping a ketosynthase:acyl carrier protein binding interface via unnatural amino acid-mediated photo-cross-linking.

    abstract::Probing and interrogating protein interactions that involve acyl carrier proteins (ACP's) in fatty acid synthases and polyketide synthases are critical to understanding the molecular basis for the programmed assembly of complex natural products. Here, we have used unnatural amino acid mutagenesis to site specifically ...


    pub_type: 杂志文章


    authors: Ye Z,Williams GJ

    更新日期:2014-12-09 00:00:00

  • Construction of cryptogein mutants, a proteinaceous elicitor from Phytophthora, with altered abilities to induce a defense reaction in tobacco cells.

    abstract::We prepared a series of cryptogein mutants, an elicitor from Phytophthora cryptogea, with altered abilities to bind sterols and fatty acids. The induction of the early events, i.e., synthesis of active oxygen species and pH changes, in suspension tobacco cells by these mutated proteins was proportional to their abilit...


    pub_type: 杂志文章


    authors: Lochman J,Kasparovsky T,Damborsky J,Osman H,Marais A,Chaloupkova R,Ponchet M,Blein JP,Mikes V

    更新日期:2005-05-03 00:00:00

  • Mechanism of tubulin assembly: guanosine 5'-triphosphate hydrolysis decreases the rate of microtubule depolymerization.

    abstract::The rate of depolymerization of microtubules upon lowering the temperature was found to depend on the amount of time elapsed since the beginning of the assembly process. In the first minutes following self-assembly at 37 degrees C, microtubules are more cold sensitive and depolymerize faster than later at the steady s...


    pub_type: 杂志文章


    authors: Bonne D,Pantaloni D

    更新日期:1982-03-02 00:00:00

  • Kinetic behavior of porcine pancreatic phospholipase A2 on zwitterionic and negatively charged double-chain substrates.

    abstract::A number of isomeric diacylglycerophosphocholines and diacylglycero sulfates containing O-acyl and/or S-acyl ester bonds were investigated as substrates for porcine pancreatic phospholipase A2 and its zymogen. A comparison is made with the kinetic properties of the enzyme toward the corresponding glycol detergents pre...


    pub_type: 杂志文章


    authors: van Oort MG,Dijkman R,Hille JD,de Haas GH

    更新日期:1985-12-31 00:00:00

  • Identification of a new P450 expressed in human lung: complete cDNA sequence, cDNA-directed expression, and chromosome mapping.

    abstract::A cDNA coding for a P450 expressed in human lung was isolated from a lambda gt11 library constructed from human lung mRNA using a cDNA probe to rat P450 IVA1. The cDNA-deduced amino acid sequence of this P450, designated IVB1, consisted of 511 amino acids and had a calculated molecular weight of 59,558. The IVB1 amino...


    pub_type: 杂志文章


    authors: Nhamburo PT,Gonzalez FJ,McBride OW,Gelboin HV,Kimura S

    更新日期:1989-10-03 00:00:00

  • 13C NMR studies of methylene and methine carbons of substrate bound to a 280,000-dalton protein, porphobilinogen synthase.

    abstract::13C NMR has been used to observe the equilibrium complex of [5,5-2H,5-13C]-5-aminolevulinate [( 5,5-2H,5-13C]ALA) bound to porphobilinogen (PBG) synthase (5-aminolevulinate dehydratase), a 280,000-dalton protein. [5,5-2H,5-13C]ALA (chemical shift 46.9 ppm in D2O) was prepared from [5-13C]ALA through enolization in deu...


    pub_type: 杂志文章


    authors: Jaffe EK,Markham GD

    更新日期:1988-06-14 00:00:00

  • Studies of 1-deoxy-D-fructose, 1-deoxy-D-glucitol, and 1-deoxy-D-minnitol as antimetabolites.

    abstract::1-Deoxy-D-fructose was synthesized in 27% yield from D-glucosamine in a three-step procedure involving Raney nickel desulfurization and oxidative deamination with 3,5-di-tert-butyl- 1,2-benzoquinone applied to appropriate intermediates. 1-Deoxyfructose and its reduction products, 1-deoxyglucitol and 1-deoxymannitol, w...


    pub_type: 杂志文章


    authors: Dills WL Jr,Meyer WL

    更新日期:1976-10-05 00:00:00

  • Chemical modification of the bovine mitochondrial bc1 complex reveals critical acidic residues involved in the proton pumping activity.

    abstract::Bovine heart ubiquinol-cytochrome c reductase (bc1 complex) was modified with N-(ethoxycarbonyl)-2-ethoxy-1,2-dihydroquinoline (EEDQ), which is a selective reagent for buried carboxyl groups. EEDQ treatment caused a loss of the proton pumping activity of liposome-reconstituted bc1 complex, without effect on the passiv...


    pub_type: 杂志文章


    authors: Cocco T,Di Paola M,Papa S,Lorusso M

    更新日期:1998-02-17 00:00:00

  • Effect of acid pH on the absorption spectra and photoreactions of bacteriorhodopsin.

    abstract::Purple membranes (PM) from Halobacterium halobium were incorporated into 7.5% polyacrylamide gels to prevent aggregation which occurs in suspensions at low pH. At pH 7.0, the circular dichroism (CD) spectra and visible absorption spectra of light- and dark-adapted bacteriorhodopsin (bR558, respectively) and the flash ...


    pub_type: 杂志文章


    authors: Mowery PC,Lozier RH,Chae Q,Tseng YW,Taylor M,Stoeckenius W

    更新日期:1979-09-18 00:00:00