Characterization of annexin A1 glycan binding reveals binding to highly sulfated glycans with preference for highly sulfated heparan sulfate and heparin.

abstract：:The metabolism of iron-sulfur ([Fe-S]) clusters requires a complex set of machinery that is still being defined. Mutants of Salmonella enterica lacking apbC have nutritional and biochemical properties indicative of defects in [Fe-S] cluster metabolism. ApbC is a 40.8 kDa homodimeric ATPase and as purified contains lit...

journal_title：Biochemistry

authors： Boyd JM,Pierik AJ,Netz DJ,Lill R,Downs DM

更新日期：2008-08-05 00:00:00

abstract：:This paper reports the first identification of a fully functional hydrolyzing UDP-N-acetylglucosamine 2-epimerase from a bacterial source. The epimerase (known as SiaA or NeuC) from Neisseria meningitidis MC58 group B is shown to catalyze the conversion of UDP-GlcNAc into ManNAc and UDP in the first step of sialic aci...

journal_title：Biochemistry

authors： Murkin AS,Chou WK,Wakarchuk WW,Tanner ME

更新日期：2004-11-09 00:00:00

abstract：:The interaction of heparan sulfate with different ligand proteins depends on the precise location of O-sulfate groups in the polysaccharide chain. We have previously shown that overexpression in human kidney 293 cells of a mouse mastocytoma 2-O-sulfotransferase (2-OST), previously thought to catalyze the transfer of s...

journal_title：Biochemistry

authors： Rong J,Habuchi H,Kimata K,Lindahl U,Kusche-Gullberg M

更新日期：2001-05-08 00:00:00

abstract：:Bacteriocins are bacterial peptides with specific activity against competing species. They hold great potential as natural preservatives and for their probiotic effects. We show here nuclear magnetic resonance-based evidence that glycocin F, a 43-amino acid bacteriocin from Lactobacillus plantarum, contains two β-link...

journal_title：Biochemistry

authors： Venugopal H,Edwards PJ,Schwalbe M,Claridge JK,Libich DS,Stepper J,Loo T,Patchett ML,Norris GE,Pascal SM

更新日期：2011-04-12 00:00:00

abstract：:The chemical mechanism of phosphodiester bond hydrolysis catalyzed by a junction-resolving enzyme has been investigated. Endonuclease I of phage T7 is a member of the nuclease superfamily of proteins that include many restriction enzymes, and the structure of the active site is very similar to that of BglI in particul...

journal_title：Biochemistry

authors： Liu J,Déclais AC,Lilley DM

更新日期：2006-03-28 00:00:00

abstract：:Translesion synthesis (TLS) is a mutagenic branch of cellular DNA damage tolerance that enables bypass replication over DNA lesions carried out by specialized low-fidelity DNA polymerases. The replicative bypass of most types of DNA damage is performed in a two-step process of Rev1/Polζ-dependent TLS. In the first ste...

journal_title：Biochemistry

authors： Pustovalova Y,Magalhães MT,D'Souza S,Rizzo AA,Korza G,Walker GC,Korzhnev DM

更新日期：2016-04-05 00:00:00

abstract：:The Sir2 enzyme family is responsible for a newly classified chemical reaction, NAD(+)-dependent protein deacetylation. New peptide substrates, the reaction mechanism, and the products of the acetyl transfer to NAD(+) are described for SIR2. The final products of SIR2 reactions are the deacetylated peptide and the 2' ...

journal_title：Biochemistry

authors： Sauve AA,Celic I,Avalos J,Deng H,Boeke JD,Schramm VL

更新日期：2001-12-25 00:00:00

abstract：:The 270-MHz 1H nuclear magnetic resonance spectra of the apo, copper(I)-zinc(II), and copper(II)-zinc(II) forms of bovine erythrocyte superoxide dismutase (EC 1.15.1.1) are reported, and assignments of resonances to ten amino acid residues are proposed. The data require that at least four and probably six histidine re...

journal_title：Biochemistry

authors： Cass AE,Hill AO,Smith BE,Bannister JV,Bannister WH

更新日期：1977-07-12 00:00:00

abstract：:A method is described for measuring equilibrium constants of DNA-protein interactions using gel chromatography. This technique has been used to study the sequence-specific interaction of the HinfI restriction endonuclease with DNA. HinfI has a monomeric molecular weight of 31000 and exists as a dimer in its active for...

journal_title：Biochemistry

authors： Frankel AD,Ackers GK,Smith HO

更新日期：1985-06-04 00:00:00

abstract：:The acetylenic analogue of adenosine 9-(5',6'-dideoxy-beta-D-ribo-hex-5'-ynofuranosyl)adenine has been synthesized, and its behavior as an inhibitor of bovine S-adenosylhomocysteine hydrolase has been examined. Incubation of the enzyme with excess inhibitor caused a time-dependent, irreversible inactivation of the enz...

journal_title：Biochemistry

authors： Parry RJ,Muscate A,Askonas LJ

更新日期：1991-10-15 00:00:00

abstract：:We have measured the base-pair lifetimes in oligodeoxynucleotides containing tracts of A.T base pairs using imino proton magnetic resonance. When the tract contains more than four consecutive A.T base pairs, possibly including a 5'-AT step but not a 5'-TA step, anomalously long lifetimes are observed. For example, the...

journal_title：Biochemistry

authors： Leroy JL,Charretier E,Kochoyan M,Guéron M

更新日期：1988-12-13 00:00:00

abstract：:Two scorpion neurotoxins active only on insects, the insect toxins AaH IT1 and AaH IT2, were purified from the venom of scorpions Androctonus australis Hector collected in Tozeur (Tunisia) and characterized. AaH IT2 was sequenced and found to differ in four amino acid positions from AaH IT, the single previously seque...

journal_title：Biochemistry

authors： Loret EP,Mansuelle P,Rochat H,Granier C

更新日期：1990-02-13 00:00:00

abstract：:Previous studies of recombinant full-length human apolipoprotein A-V (apoA-V) provided evidence of the presence of two independently folded structural domains. Computer-assisted sequence analysis and limited proteolysis studies identified an N-terminal fragment as a candidate for one of the domains. C-Terminal truncat...

journal_title：Biochemistry

authors： Wong K,Beckstead JA,Lee D,Weers PM,Guigard E,Kay CM,Ryan RO

更新日期：2008-08-19 00:00:00

abstract：:The extent of rapid (picosecond) backbone motions within the glucocorticoid receptor DNA-binding domain (GR DBD) has been investigated using proton-detected heteronuclear NMR spectroscopy on uniformly 15N-labeled protein fragments containing the GR DBD. Sequence-specific 15N resonance assignments, based on two- and th...

journal_title：Biochemistry

authors： Berglund H,Kovács H,Dahlman-Wright K,Gustafsson JA,Härd T

更新日期：1992-12-08 00:00:00

abstract：:The central importance of chorismate enzymes in bacteria, fungi, parasites, and plants combined with their absence in mammals makes them attractive targets for antimicrobials and herbicides. Two of these enzymes, anthranilate synthase (AS) and aminodeoxychorismate synthase (ADCS), are structurally and mechanistically ...

journal_title：Biochemistry

authors： Culbertson JE,Chung Dh,Ziebart KT,Espiritu E,Toney MD

更新日期：2015-04-14 00:00:00

abstract：:A manganese-dependent 3,4-dihydroxyphenylactate 2,3-dioxygenase from Arthrobacter globiformis strain CM-2 (MndD) cloned in Escherichia coli has been purified to homogeneity. Sedimentation equilibrium analysis indicates an alpha 4 homotetrameric holoenzyme structure (4 x 38,861 Da). Steady-state kinetic analysis of Mnd...

journal_title：Biochemistry

authors： Whiting AK,Boldt YR,Hendrich MP,Wackett LP,Que L Jr

更新日期：1996-01-09 00:00:00

abstract：:The active-site zinc in human glutathione-dependent formaldehyde dehydrogenase (FDH) undergoes coenzyme-induced displacement and transient coordination to a highly conserved glutamate residue (Glu-67) during the catalytic cycle. The role of this transient coordination of the active-site zinc to Glu-67 in the FDH catal...

journal_title：Biochemistry

authors： Sanghani PC,Davis WI,Zhai L,Robinson H

更新日期：2006-04-18 00:00:00

abstract：:The kinetics of human polymerase beta (pol beta) binding to the single-stranded DNA, in the (pol beta)(16) and (pol beta)(5) binding modes, that differ in the number of occluded nucleotide residues in the protein-DNA complexes, have been examined, using the fluorescence stopped-flow technique. This is the first determ...

journal_title：Biochemistry

authors： Rajendran S,Jezewska MJ,Bujalowski W

更新日期：2001-10-02 00:00:00

abstract：:Rabbit muscle myosin S1 was modified either at SH1 alone or at both SH1 and SH2, using a series of alkylthiolating reagents of increasing size, designed for correlating gradually changing structural disturbances in the thiol region with functional impairments in the myosin head. The reagents were of the type H(CH(2))(...

journal_title：Biochemistry

authors： Tiepold M,Kliche W,Pfannstiel J,Faulstich H

更新日期：2000-02-15 00:00:00

abstract：:Deuterium quadrupole echo spectra and spin-lattice relaxation rates measured at 76.8 and 38.4 MHz as a function of relative humidity are reported for calf thymus DNA deuterated at positions A8 and G8. The amplitude of base pair motion is observed to increase slightly with increasing degree of hydration (up to approxim...

journal_title：Biochemistry

authors： Brandes R,Vold RR,Vold RL,Kearns DR

更新日期：1986-11-18 00:00:00

abstract：:Phosphorylase kinase, a regulatory enzyme of glycogenolysis in skeletal muscle, is a hexadecameric oligomer consisting of four copies each of a catalytic subunit (gamma) and three regulatory subunits (alpha, beta, and delta, the last being endogenous calmodulin). The enzyme is activated by a variety of effectors actin...

journal_title：Biochemistry

authors： Nadeau OW,Traxler KW,Fee LR,Baldwin BA,Carlson GM

更新日期：1999-02-23 00:00:00

abstract：:Probing and interrogating protein interactions that involve acyl carrier proteins (ACP's) in fatty acid synthases and polyketide synthases are critical to understanding the molecular basis for the programmed assembly of complex natural products. Here, we have used unnatural amino acid mutagenesis to site specifically ...

journal_title：Biochemistry

authors： Ye Z,Williams GJ

更新日期：2014-12-09 00:00:00

abstract：:We prepared a series of cryptogein mutants, an elicitor from Phytophthora cryptogea, with altered abilities to bind sterols and fatty acids. The induction of the early events, i.e., synthesis of active oxygen species and pH changes, in suspension tobacco cells by these mutated proteins was proportional to their abilit...

journal_title：Biochemistry

authors： Lochman J,Kasparovsky T,Damborsky J,Osman H,Marais A,Chaloupkova R,Ponchet M,Blein JP,Mikes V

更新日期：2005-05-03 00:00:00

abstract：:The rate of depolymerization of microtubules upon lowering the temperature was found to depend on the amount of time elapsed since the beginning of the assembly process. In the first minutes following self-assembly at 37 degrees C, microtubules are more cold sensitive and depolymerize faster than later at the steady s...

journal_title：Biochemistry

authors： Bonne D,Pantaloni D

更新日期：1982-03-02 00:00:00

abstract：:A number of isomeric diacylglycerophosphocholines and diacylglycero sulfates containing O-acyl and/or S-acyl ester bonds were investigated as substrates for porcine pancreatic phospholipase A2 and its zymogen. A comparison is made with the kinetic properties of the enzyme toward the corresponding glycol detergents pre...

journal_title：Biochemistry

authors： van Oort MG,Dijkman R,Hille JD,de Haas GH

更新日期：1985-12-31 00:00:00

abstract：:A cDNA coding for a P450 expressed in human lung was isolated from a lambda gt11 library constructed from human lung mRNA using a cDNA probe to rat P450 IVA1. The cDNA-deduced amino acid sequence of this P450, designated IVB1, consisted of 511 amino acids and had a calculated molecular weight of 59,558. The IVB1 amino...

journal_title：Biochemistry

authors： Nhamburo PT,Gonzalez FJ,McBride OW,Gelboin HV,Kimura S

更新日期：1989-10-03 00:00:00

abstract：:13C NMR has been used to observe the equilibrium complex of [5,5-2H,5-13C]-5-aminolevulinate [( 5,5-2H,5-13C]ALA) bound to porphobilinogen (PBG) synthase (5-aminolevulinate dehydratase), a 280,000-dalton protein. [5,5-2H,5-13C]ALA (chemical shift 46.9 ppm in D2O) was prepared from [5-13C]ALA through enolization in deu...

journal_title：Biochemistry

authors： Jaffe EK,Markham GD

更新日期：1988-06-14 00:00:00

abstract：:1-Deoxy-D-fructose was synthesized in 27% yield from D-glucosamine in a three-step procedure involving Raney nickel desulfurization and oxidative deamination with 3,5-di-tert-butyl- 1,2-benzoquinone applied to appropriate intermediates. 1-Deoxyfructose and its reduction products, 1-deoxyglucitol and 1-deoxymannitol, w...

journal_title：Biochemistry

authors： Dills WL Jr,Meyer WL

更新日期：1976-10-05 00:00:00

abstract：:Bovine heart ubiquinol-cytochrome c reductase (bc1 complex) was modified with N-(ethoxycarbonyl)-2-ethoxy-1,2-dihydroquinoline (EEDQ), which is a selective reagent for buried carboxyl groups. EEDQ treatment caused a loss of the proton pumping activity of liposome-reconstituted bc1 complex, without effect on the passiv...

journal_title：Biochemistry

authors： Cocco T,Di Paola M,Papa S,Lorusso M

更新日期：1998-02-17 00:00:00

abstract：:Purple membranes (PM) from Halobacterium halobium were incorporated into 7.5% polyacrylamide gels to prevent aggregation which occurs in suspensions at low pH. At pH 7.0, the circular dichroism (CD) spectra and visible absorption spectra of light- and dark-adapted bacteriorhodopsin (bR558, respectively) and the flash ...

journal_title：Biochemistry

authors： Mowery PC,Lozier RH,Chae Q,Tseng YW,Taylor M,Stoeckenius W

更新日期：1979-09-18 00:00:00