Residues 207, 216, and 221 and the catalytic activity of mGSTA1-1 and mGSTA2-2 toward benzo[a]pyrene-(7R,8S)-diol-(9S,10R)-epoxide.

abstract：:alpha-1,3-Fucosyltransferase V (FucT V) catalyzes the transfer of 1-fucose from the donor sugar guanosine 5'-diphospho-beta-1-fucose (GDP-Fuc) to an acceptor sugar. A secondary isotope effect on the fucosyltransfer reaction with guanosine 5'-diphospho-[1-2H]-beta-1-fucose (GDP-[1-2H]-Fuc) as the substrate was observed...

journal_title：Biochemistry

authors： Murray BW,Wittmann V,Burkart MD,Hung SC,Wong CH

更新日期：1997-01-28 00:00:00

abstract：:Phosphinothricyl-alanyl-alanine (Pt tripeptide (Ptt), bialaphos) is a metabolite produced by Streptomyces viridochromogenes and Streptomyces hygroscopicus. It contains the unique phosphinoamino acid phosphinothricin (Pt), which after cleavage from Ptt is active as an inhibitor of glutamine synthetase. We have isolated...

journal_title：Biochemistry

authors： Grammel N,Schwartz D,Wohlleben W,Keller U

更新日期：1998-02-10 00:00:00

abstract：:In this report recombinant estrogen binding protein (EBP1), isolated originally from Candida albicans as a result of its high affinity for 17beta-estradiol, has been purified extensively using a modified affinity purification scheme originally developed for a homolog of EBP1, old yellow enzyme (OYE). It is shown that ...

journal_title：Biochemistry

authors： Buckman J,Miller SM

更新日期：1998-10-06 00:00:00

abstract：:Specific binding of glutamate to its carrier was investigated by using cytoplasmic membrane vesicles prepared from Escherichia coli B. The binding activity was specifically affected by the Na+ and H+ concentrations of the medium. Cytoplasmic membrane vesicles from the mutant strain 36-39 that is defective in the Na+-d...

journal_title：Biochemistry

authors： Fujimura T,Yamato I,Anraku Y

更新日期：1983-04-12 00:00:00

abstract：:Legumain (asparaginyl endopeptidase) is the only protease with a preference for cleavage after asparagine residues. Increased legumain activity is a hallmark of inflammation, neurodegenerative diseases, and cancer, and legumain inhibitors have exhibited therapeutic effects in mouse models of these pathologies. Improve...

journal_title：Biochemistry

authors： Anderson BM,de Almeida LGN,Sekhon H,Young D,Dufour A,Edgington-Mitchell LE

更新日期：2020-01-28 00:00:00

abstract：:Tryptophan 2,3-dioxygenase (TDO) from Xanthomonas campestris is a highly specific heme-containing enzyme from a small family of homologous enzymes, which includes indoleamine 2,3-dioxygenase (IDO). The structure of wild type (WT TDO) in the catalytically active, ferrous (Fe (2+)) form and in complex with its substrate...

journal_title：Biochemistry

authors： Thackray SJ,Bruckmann C,Anderson JL,Campbell LP,Xiao R,Zhao L,Mowat CG,Forouhar F,Tong L,Chapman SK

更新日期：2008-10-07 00:00:00

abstract：:Cytochrome P450 BM-3 is a bacterial enzyme with sequence similarity to mammalian P450s that catalyzes the hydroxylation of fatty acids with high efficiency. Enzyme-substrate binding and dynamics has been an important topic of study for cytochromes P450 because most of the crystal structures of substrate-bound structur...

journal_title：Biochemistry

authors： Catalano J,Sadre-Bazzaz K,Amodeo GA,Tong L,McDermott A

更新日期：2013-10-01 00:00:00

abstract：:We have investigated the interaction of alpha 2-macroglobulin (alpha 2M) with the serine proteinase urokinase, an activator of plasminogen. Urokinase formed sodium dodecyl sulfate stable complexes with purified alpha 2M and with alpha 2M in plasma. These complexes could be visualized after polyacrylamide gel electroph...

journal_title：Biochemistry

authors： Straight DL,Hassett MA,McKee PA

更新日期：1985-07-16 00:00:00

abstract：:The structural protein nonerythroid alpha spectrin (alphaIISp) plays a role in the repair of DNA interstrand cross-links and is deficient in cells from patients with Fanconi anemia (FA), in which there is a defect in ability to repair such cross-links. We have proposed a model in which alphaIISp, whose stability is de...

journal_title：Biochemistry

authors： Lefferts JA,Wang C,Sridharan D,Baralt M,Lambert MW

更新日期：2009-01-20 00:00:00

abstract：:Activated receptors with a tyrosine kinase activity induce a variety of responses like changes in the differentiation and mitogenic status of cells. These responses are mediated in part by p21ras. Some of these activated receptors induce in certain cell types a pronounced, but transient, increase in Ras-GTP. We have s...

journal_title：Biochemistry

authors： Osterop AP,Medema RH,Ouwens DM,Van der Zon GC,Möller W,Maassen JA

更新日期：1994-06-14 00:00:00

abstract：:To study the effects of methylation damage on DNA replication in vitro, the plasmid pSVori containing the SV40 origin of replication was reacted with N-methyl-N-nitrosourea and used as a substrate for SV40 T antigen dependent replication by HeLa cell extracts. The plasmid was methylated with a range of N-methyl-N-nitr...

journal_title：Biochemistry

authors： Ceccotti S,Dogliotti E,Gannon J,Karran P,Bignami M

更新日期：1993-12-14 00:00:00

abstract：:Lipocortin I is a 39-kilodalton membrane-associated protein that in A431 cells is phosphorylated on tyrosine in response to epidermal growth factor (EGF). We have used recombinant human lipocortin I as a substrate for several protein kinases and identified phosphorylated residues by a combination of peptide mapping an...

journal_title：Biochemistry

authors： Varticovski L,Chahwala SB,Whitman M,Cantley L,Schindler D,Chow EP,Sinclair LK,Pepinsky RB

更新日期：1988-05-17 00:00:00

abstract：:In aqueous glycerol solutions, the rate constant for the formation of compound I of horseradish peroxidase from hydrogen peroxide is independent of the viscosity. However, whem m-chloroperbenzoic acid is used to form compound I, the rate is viscosity dependent, indicating that this reaction is diffusion controlled. Th...

journal_title：Biochemistry

authors： Dunford HB,Hewson WD

更新日期：1977-06-28 00:00:00

abstract：:Cdc31, the Saccharomyces cerevisiae centrin, is an EF-hand calcium-binding protein essential for the cell division and mRNA nuclear export. We used biophysical techniques to investigate its calcium, magnesium, and protein target binding properties as well as their conformations in solution. We show here that Cdc31 dis...

journal_title：Biochemistry

authors： Miron S,Durand D,Chilom C,Pérez J,Craescu CT

更新日期：2011-07-26 00:00:00

abstract：:Five gangliosides, accounting for over 95% of the total ganglioside fraction, were isolated from bovine adrenal medulla by preparative thin-layer chromatography and the carbohydrate structures determined by a combination of periodate oxidation and permethylation techniques. Partially methylated alditol acetates were g...

journal_title：Biochemistry

authors： Price H,Kundu S,Ledeen R

更新日期：1975-04-08 00:00:00

abstract：:Over 70 transthyretin (TTR) mutations facilitate amyloidosis in tissues other than the central nervous system (CNS). In contrast, the D18G TTR mutation in individuals of Hungarian descent leads to CNS amyloidosis. D18G forms inclusion bodies in Escherichia coli, unlike the other disease-associated TTR variants overexp...

journal_title：Biochemistry

authors： Hammarström P,Sekijima Y,White JT,Wiseman RL,Lim A,Costello CE,Altland K,Garzuly F,Budka H,Kelly JW

更新日期：2003-06-10 00:00:00

abstract：:The catalytic utilization of dimethylallyl, geranyl, farnesyl, and geranylgeranyl diphosphates in the reaction catalyzed by recombinant human farnesyl:protein transferase (hFPTase) has been examined in the presence of three different protein substrates, Ras-CVLS, Ras-CVIM, and Ras-CAIL. hFPTase catalyzed both farnesyl...

journal_title：Biochemistry

authors： Pompliano DL,Schaber MD,Mosser SD,Omer CA,Shafer JA,Gibbs JB

更新日期：1993-08-17 00:00:00

abstract：:The backbone dynamics of the liganded (holo) and unliganded (apo) forms of Escherichia coli-derived rat intestinal fatty acid-binding protein (I-FABP) have been characterized and compared using amide 15N relaxation and 1H exchange NMR measurements. The amide 1H/15N resonances for apo and holo I-FABP were assigned at 2...

journal_title：Biochemistry

authors： Hodsdon ME,Cistola DP

更新日期：1997-02-25 00:00:00

abstract：:Gelatinase B is a matrix metalloproteinase (MMP-9) involved in tissue remodeling, development, cancer, and inflammation. Neutrophils produce three major forms of (pro)gelatinase B: 92 kDa monomers, homodimers, and complexes of gelatinase B covalently bound to neutrophil gelatinase B-associated lipocalin (NGAL). In con...

journal_title：Biochemistry

authors： Rudd PM,Mattu TS,Masure S,Bratt T,Van den Steen PE,Wormald MR,Küster B,Harvey DJ,Borregaard N,Van Damme J,Dwek RA,Opdenakker G

更新日期：1999-10-19 00:00:00

abstract：:Because of its ability to selectively glucosylate misfolded glycoproteins, UDP-glucose:glycoprotein glucosyltransferase (UGGT) functions as a folding sensor in the glycoprotein quality control system in the endoplasmic reticulum (ER). The unique property of UGGT derives from its ability to transfer a glucose residue t...

journal_title：Biochemistry

authors： Ohara K,Takeda Y,Daikoku S,Hachisu M,Seko A,Ito Y

更新日期：2015-08-11 00:00:00

abstract：:Glutaraldehyde treatment of electroplax membrane preparations of Na,K-ATPase leads to irreversible changes in the enzymic behavior of the protein, which are not due to modification of the active site. When the glutaraldehyde treatment is carried out in a medium containing K+ and without Na+, the "K+-modified enzyme" s...

journal_title：Biochemistry

authors： Chipman DM,Lev A

更新日期：1983-09-13 00:00:00

abstract：:The second-order rate constants for the reactions between nitrogen monoxide and oxymyoglobin or oxyhemoglobin, determined by stopped-flow spectroscopy, increase with increasing pH. At pH 7.0 the rates are (43.6 +/- 0.5) x 10(6) M(-1) x s(-1) for oxymyoglobin and (89 +/- 3) x 10(6) M(-1) x s(-1) for oxyhemoglobin (per ...

journal_title：Biochemistry

authors： Herold S,Exner M,Nauser T

更新日期：2001-03-20 00:00:00

abstract：:Proton inventories (rate measurements in mixtures of H2O and D2O) were determined for the human leukocyte elastase catalyzed hydrolyses of thiobenzyl esters and p-nitroanilides of the peptides MeOSuc-Val, MeOSuc-Alan-Pro-Val (n = 0-2), and MeOSuc-Alan-Pro-Ala (n = 1 or 2). The dependencies of k2/Ks on mole fraction of...

journal_title：Biochemistry

authors： Stein RL,Strimpler AM,Hori H,Powers JC

更新日期：1987-03-10 00:00:00

abstract：:Haloalkane dehalogenase converts haloalkanes to their corresponding alcohols and halides. The reaction mechanism involves the formation of a covalent alkyl-enzyme complex which is hydrolyzed by water. The active site is a hydrophobic cavity buried between the main domain and the cap domain of the enzyme. The enzyme ha...

journal_title：Biochemistry

authors： Schanstra JP,Janssen DB

更新日期：1996-05-07 00:00:00

abstract：:To elucidate the mechanism of solute diffusion through lipid bilayer membranes, nearly 4 ns of molecular dynamics simulations of solutes in phospholipid bilayers was conducted. The study, the first atomic level study of solute diffusion in a lipid bilayer, involved four simulations of an all-atom representation of a f...

journal_title：Biochemistry

authors： Bassolino-Klimas D,Alper HE,Stouch TR

更新日期：1993-11-30 00:00:00

abstract：:Sarcoplasmic phosphorylase phosphatase extracted from ground skeletal muscle was recovered in a high molecular weight from (Mr = 250000). This enzyme has been purified from extracts by anion-exchange and gel chromatography to yield a preparation with three major protein components of Mr 83000, 72000, and 32000 by sodi...

journal_title：Biochemistry

authors： Brautigan DL,Picton C,Fischer EH

更新日期：1980-12-09 00:00:00

abstract：:Betaine-homocysteine S-methyltransferase (BHMT) is a zinc-dependent enzyme that catalyzes the transfer of a methyl group from glycine betaine (Bet) to homocysteine (Hcy) to form dimethylglycine (DMG) and methionine (Met). Previous studies in other laboratories have indicated that catalysis proceeds through the formati...

journal_title：Biochemistry

authors： Castro C,Gratson AA,Evans JC,Jiracek J,Collinsová M,Ludwig ML,Garrow TA

更新日期：2004-05-11 00:00:00

abstract：:A newly recognized type of protein-ligand interaction phenomenon has resulted in the preparation of simple, nonionic, and highly specific gel derivatives for selective adsorption chromatography. The essential structure of the immobilized ligand can be represented as agarose-CH2CH2SO2CH2CH2SCH2CH2OH, which was prepared...

journal_title：Biochemistry

authors： Hutchens TW,Porath J

更新日期：1987-11-03 00:00:00

abstract：:Because of the lack of protein turnover in fiber cells of the ocular lens, Aquaporin 0 (AQP0), the most abundant membrane protein in the lens, undergoes extensive post-translational modification with fiber cell age. To map the distribution of modified forms of AQP0 within the lens, normal human lenses ranging in age f...

journal_title：Biochemistry

authors： Ball LE,Garland DL,Crouch RK,Schey KL

更新日期：2004-08-03 00:00:00

abstract：:Understanding the evolution of allostery in multidomain enzymes remains an important step in improving our ability to identify and exploit structure-function relationships in allosteric mechanisms. A recent protein similarity network for the DRE-TIM metallolyase superfamily indicated there are two evolutionarily disti...

journal_title：Biochemistry

authors： Kumar G,Frantom PA

更新日期：2014-07-29 00:00:00