Ligand binding alters the backbone mobility of intestinal fatty acid-binding protein as monitored by 15N NMR relaxation and 1H exchange.


:The backbone dynamics of the liganded (holo) and unliganded (apo) forms of Escherichia coli-derived rat intestinal fatty acid-binding protein (I-FABP) have been characterized and compared using amide 15N relaxation and 1H exchange NMR measurements. The amide 1H/15N resonances for apo and holo I-FABP were assigned at 25 degrees C, and gradient- and sensitivity-enhanced 2D experiments were employed to measure l5N T1, T2, and [1H]15N NOE values and relative 1H saturation transfer rates. The 15N relaxation parameters were analyzed using five different representations of the spectral density function based on the Lipari and Szabo formalism. A majority of the residues in both apo and holo I-FABP were characterized by relatively slow hydrogen exchange rates, high generalized order parameters, and no conformational exchange terms. However, residues V26-N35, S53-R56, and A73-T76 of apo I-FABP were characterized by rapid hydrogen exchange, low order parameters, and significant conformational exchange. These residues are clustered in a single region of the protein where variability and apparent disorder were previously observed in the chemical shift analyses and in the NOE-derived NMR structures of apo I-FABP. The increased mobility and discrete disorder in the backbone of the apo protein may permit the entry of ligand into the binding cavity. We postulate that the bound fatty acid participates in a series of long-range cooperative interactions that cap and stabilize the C-terminal half of helix II and lead to an ordering of the portal region. This ligand-modulated order-disorder transition has implications for the role of I-FABP in cellular fatty acid transport and targeting.






Hodsdon ME,Cistola DP




Has Abstract


1997-02-25 00:00:00














  • Formation of monomeric S100B and S100A11 proteins at low ionic strength.

    abstract::The S100 proteins comprise a group of EF-hand proteins that undergo a calcium-induced conformational change allowing them to interact with other proteins and produce a biological response. A unique feature of these proteins is the fact that they can form both homo- and heterodimers independent of calcium binding. The ...


    pub_type: 杂志文章


    authors: Marlatt NM,Boys BL,Konermann L,Shaw GS

    更新日期:2009-03-10 00:00:00

  • Preferential binding of H1e histone to GC-rich DNA.

    abstract::We have investigated the binding of a pure H1 histone, the mouse variant H1e, to a 214 bp fragment of DNA from pBR322. Binding was monitored by observing the effects of the protein on melting of the DNA and by a gel-mobility-shift assay. We found, using this highly purified system, that H1e protein binds preferentiall...


    pub_type: 杂志文章


    authors: Wellman SE,Sittman DB,Chaires JB

    更新日期:1994-01-11 00:00:00

  • Crystal structures and mutational analyses of acyl-CoA carboxylase beta subunit of Streptomyces coelicolor.

    abstract::The first committed step of fatty acid and polyketides biosynthesis, the biotin-dependent carboxylation of an acyl-CoA, is catalyzed by acyl-CoA carboxylases (ACCases) such as acetyl-CoA carboxylase (ACC) and propionyl-CoA carboxylase (PCC). ACC and PCC in Streptomyces coelicolor are homologue multisubunit complexes t...


    pub_type: 杂志文章


    authors: Arabolaza A,Shillito ME,Lin TW,Diacovich L,Melgar M,Pham H,Amick D,Gramajo H,Tsai SC

    更新日期:2010-08-31 00:00:00

  • Targeted generation of DNA strand breaks using pyrene-conjugated triplex-forming oligonucleotides.

    abstract::Gene targeting by triplex-forming oligonucleotides (TFOs) has proven useful for gene modulation in vivo. Photoreactive molecules have been conjugated to TFOs to direct sequence-specific damage in double-stranded DNA. However, the photoproducts are often repaired efficiently in cells. This limitation has led to the sea...


    pub_type: 杂志文章


    authors: Benfield AP,Macleod MC,Liu Y,Wu Q,Wensel TG,Vasquez KM

    更新日期:2008-06-10 00:00:00

  • Engineered biosynthesis of novel polyketides: regiospecific methylation of an unnatural substrate by the tcmO O-methyltransferase.

    abstract::TcmO is an O-methyltransferase that methylates the C-8 hydroxyl to Tcm B3, a four-ring aromatic intermediate in the tetracenomycin biosynthetic pathway of Streptomyces glaucescens. The gene encoding this enzyme was expressed in Streptomyces coelicolor CH999 together with the actinorhodin polyketide synthase (PKS) gene...


    pub_type: 杂志文章


    authors: Fu H,Alvarez MA,Khosla C,Bailey JE

    更新日期:1996-05-28 00:00:00

  • In silico mutation of cysteine residues in the ligand-binding domain of an N-methyl-D-aspartate receptor.

    abstract::The precise nature of redox modulation of N-methyl-d-aspartate (NMDA) receptors is still unclear, although it is thought to be related to the formation and breaking of disulfide bonds. Recent structural data demonstrated the way in which disulfide bonds in the ligand-binding core of the NR1 subunit are arranged. Howev...


    pub_type: 杂志文章


    authors: Kaye SL,Sansom MS,Biggin PC

    更新日期:2007-02-27 00:00:00

  • Solution structure of the Dickerson DNA dodecamer containing a single ribonucleotide.

    abstract::Ribonucleotides are frequently incorporated into DNA during replication. They are recognized and processed by several cellular enzymes, and their continued presence in the yeast nuclear genome results in replicative stress and genome instability. Thus, it is important to understand the effects of isolated ribonucleoti...


    pub_type: 杂志文章


    authors: DeRose EF,Perera L,Murray MS,Kunkel TA,London RE

    更新日期:2012-03-27 00:00:00

  • Kinetic mechanism for the flipping and excision of 1,N(6)-ethenoadenine by AlkA.

    abstract::Escherichia coli 3-methyladenine DNA glycosylase II (AlkA), an adaptive response glycosylase with a broad substrate range, initiates base excision repair by flipping a lesion out of the DNA duplex and hydrolyzing the N-glycosidic bond. We used transient and steady state kinetics to determine the minimal mechanism for ...


    pub_type: 杂志文章


    authors: Taylor EL,O'Brien PJ

    更新日期:2015-01-27 00:00:00

  • New clevable photoreactive heterobifunctional cross-linking reagents for studying membrane organization.

    abstract::The synthesis is described of four new cleavable, photosensitive, heterobifunctional cross-linking reagents for use in examining membrane organization: 4'-azidoazobenzene-4oxysuccinimide ester (1), N-[4-(p-azidophenylazo)benzoyl]-3-aminopropyl-N'-oxysuccinimide ester (2), N-[4-(p-azidophenylazo)benzoyl]-6-aminohexyl-N...


    pub_type: 杂志文章


    authors: Jaffe CL,Lis H,Sharon N

    更新日期:1980-09-16 00:00:00

  • Characterization of the nuclease activity of Drosophila Rrp1 on phosphoglycolate- and phosphate-modified DNA 3'-termini.

    abstract::Drosophila Rrp1 includes a carboxy-terminal region homologous to Escherichia coli exonuclease III which is sufficient to repair both oxidative and alkylation damage to DNA. An apurinic/apyrimidinic endonuclease activity intrinsic to Rrp1 was characterized previously. In this work, the 3'-phosphodiesterase and 3'-phosp...


    pub_type: 杂志文章


    authors: Sander M,Huang SM

    更新日期:1995-01-31 00:00:00

  • Studies of the interaction of the viral suppressor of RNA silencing protein p19 with small RNAs using fluorescence polarization.

    abstract::Tombusviruses use a 19 kDa protein (p19) as a suppressor of the RNA silencing pathway during infection. The p19 protein binds to short-interfering RNA (siRNA) as a dimer and shows a high selectivity for short duplex RNAs over other RNA species. Since p19 can bind to synthetic and RNA silencing generated small RNAs wit...


    pub_type: 杂志文章


    authors: Cheng J,Sagan SM,Jakubek ZJ,Pezacki JP

    更新日期:2008-08-05 00:00:00

  • Rhodamine 123 binds to multiple sites in the multidrug resistance protein (MRP1).

    abstract::The mechanisms of MRP1-drug binding and transport are not clear. In this study, we have characterized the interaction between MRP1 and rhodamine 123 (Rh123) using the photoreactive-iodinated analogue, [(125)I]iodoaryl azido-rhodamine 123 (or IAARh123). Photoaffinity labeling of plasma membranes from HeLa cells transfe...


    pub_type: 杂志文章


    authors: Daoud R,Kast C,Gros P,Georges E

    更新日期:2000-12-19 00:00:00

  • Efficient substrate cleavage catalyzed by hammerhead ribozymes derivatized with selenium for X-ray crystallography.

    abstract::Because oxygen and selenium are in the same group (Family VI) in the periodic table, the site-specific mutagenesis at the atomic level by replacing RNA oxygen with selenium can provide insights on the structure and function of catalytic RNAs. We report here the first Se-derivatized ribozymes transcribed with all nucle...


    pub_type: 杂志文章


    authors: Brandt G,Carrasco N,Huang Z

    更新日期:2006-07-25 00:00:00

  • Effect of mixed solvents on the formation of horseradish peroxidase compound I. The importance of diffusion-controlled reactions.

    abstract::In aqueous glycerol solutions, the rate constant for the formation of compound I of horseradish peroxidase from hydrogen peroxide is independent of the viscosity. However, whem m-chloroperbenzoic acid is used to form compound I, the rate is viscosity dependent, indicating that this reaction is diffusion controlled. Th...


    pub_type: 杂志文章


    authors: Dunford HB,Hewson WD

    更新日期:1977-06-28 00:00:00

  • Structural and catalytic properties of hydrogenase from Chromatium.

    abstract::The enzyme hydrogenase, from the photosynthetic bacterium Chromatium, was purified to homogeneity after solubilization of the particulate enzyme with deoxycholate. The purification procedure included ammonium sulfate fractionation, treatment with manganous phosphate gel, heating at 63 degrees, DEAE-cellulose chromatog...


    pub_type: 杂志文章


    authors: Gitlitz PH,Krasna AI

    更新日期:1975-06-17 00:00:00

  • Mass spectrometric based mapping of the disulfide bonding patterns of integrin alpha chains.

    abstract::Integrins are one of the major mediators of cellular adherence. Structurally the component alpha and beta chains are characterized by extensive intrachain disulfide bonding. The assignment of these bonds is currently based on homology with the chains of the integrin alphaIIbbeta3. However, recent crystallographic anal...


    pub_type: 杂志文章


    authors: Krokhin OV,Cheng K,Sousa SL,Ens W,Standing KG,Wilkins JA

    更新日期:2003-11-11 00:00:00

  • Picosecond absorption studies on rhodopsin and isorhodopsin in detergent and native membrane.

    abstract::Picosecond transient absorption spectra of rhodopsin and isorhodopsin were measured at room temperature with a double-beam laser spectrophotometer after excitation at 355 nm. Photolysis studies were performed on rhodopsin solubilized in two different detergents (digitonin and 3-[(3-cholamidopropyl)dimethylammonio]-1-p...


    pub_type: 杂志文章


    authors: Rudzki JE,Peters KS

    更新日期:1984-08-14 00:00:00

  • Position-dependent local motions in spin-labeled analogues of a short alpha-helical peptide determined by electron spin resonance.

    abstract::We have used electron spin resonance and circular dichroism to examine and compare the dynamics in two analogues of the Ala-based 3K(I) peptide [Marqusee, S., Robbins, V.H., & Baldwin, R. L. (1989) Proc. Natl. Acad. Sci. U.S.A. 86, 5286-5290], labeled at positions 4 and 8, throughout the alpha-helix----coil transition...


    pub_type: 杂志文章


    authors: Miick SM,Todd AP,Millhauser GL

    更新日期:1991-10-01 00:00:00

  • Characterization of Acyl Carrier Protein-Dependent Glycosyltransferase in Mitomycin C Biosynthesis.

    abstract::Mitomycins make up a group of antitumor natural products that are biosynthesized from aminohydroxybenzoic acid (AHBA) and N-acetylglucosamine (GlcNAc). While the biosynthetic gene cluster was reported two decades ago, the mechanism by which the two building blocks, AHBA and GlcNAc, are coupled during biosynthesis rema...


    pub_type: 杂志文章


    authors: Nguyen HP,Yokoyama K

    更新日期:2019-06-25 00:00:00

  • A deuterium and phosphorus-31 nuclear magnetic resonance study of the interaction of melittin with dimyristoylphosphatidylcholine bilayers and the effects of contaminating phospholipase A2.

    abstract::The interaction of bee venom melittin with dimyristolphosphatidylcholine (DMPC) selectively deuteriated in the choline head group has been studied by deuterium and phosphorus-31 nuclear magnetic resonance (NMR) spectroscopy. The action of residual phospholipase A2 in melittin samples resulted in mixtures of DMPC and i...


    pub_type: 杂志文章


    authors: Dempsey CE,Watts A

    更新日期:1987-09-08 00:00:00

  • Effect of RNA secondary structure on the kinetics of DNA synthesis catalyzed by HIV-1 reverse transcriptase.

    abstract::The effect of RNA secondary structure on the kinetics of DNA synthesis catalyzed by HIV-1 RT was determined using a 66 nucleotide RNA template containing a stable 12 base pair hairpin structure. Prior to reaching the hairpin structure, the primer elongation by RT was fast and the kinetics of polymerization was not aff...


    pub_type: 杂志文章


    authors: Suo Z,Johnson KA

    更新日期:1997-10-14 00:00:00

  • Fidelity of in vitro DNA strand transfer reactions catalyzed by HIV-1 reverse transcriptase.

    abstract::The fidelity of DNA strand transfer reactions catalyzed by human immunodeficiency virus type 1 reverse transcriptase has been studied in vitro. A model system involving two sequential DNA strand transfers was developed to simulate the process of forced copy-choice recombination. A propensity for nucleotide misincorpor...


    pub_type: 杂志文章


    authors: Peliska JA,Benkovic SJ

    更新日期:1994-04-05 00:00:00

  • Evidence for a recombination-independent pathway for the repair of DNA interstrand cross-links based on a site-specific study with nitrogen mustard.

    abstract::DNA-DNA interstrand cross-links are thought to be important for the cytotoxicity of many chemotherapeutic agents. To study this more definitively, adduct site-specific methods are used to construct a plasmid with a single nitrogen mustard interstrand cross-link (inter-HN2-pTZSV28). Replication efficiency (RE = [coloni...


    pub_type: 杂志文章


    authors: Berardini M,Mackay W,Loechler EL

    更新日期:1997-03-25 00:00:00

  • Modification of aldose reductase by S-nitrosoglutathione.

    abstract::Kinetic and structural changes in recombinant human aldose reductase (AR) due to modification by S-nitrosoglutathione (GSNO) were investigated. Incubation of the enzyme with 10-50 microM GSNO led to a time- and concentration-dependent inactivation of the enzyme, with a second-order rate constant of 0.087 +/- 0.009 M-1...


    pub_type: 杂志文章


    authors: Chandra A,Srivastava S,Petrash JM,Bhatnagar A,Srivastava SK

    更新日期:1997-12-16 00:00:00

  • Kinetic and modeling studies of S3-S3' subsites of HIV proteinases.

    abstract::Kinetic analysis and modeling studies of HIV-1 and HIV-2 proteinases were carried out using the oligopeptide substrate [formula: see text] and its analogs containing single amino acid substitutions in P3-P3' positions. The two proteinases acted similarly on the substrates except those having certain hydrophobic amino ...


    pub_type: 杂志文章


    authors: Tözsér J,Weber IT,Gustchina A,Bláha I,Copeland TD,Louis JM,Oroszlan S

    更新日期:1992-05-26 00:00:00

  • Adenosine 5'-triphosphate analogues as structural probes for Escherichia coli glutamine synthetase.

    abstract::Introduction of specific structural probes into substrate binding sites of Escherichia coli glutamine synthetase is now possible. Various analogues of ATP substituted with an amino or sulfhydryl moiety at the 6- or 8-position of the purine ring have been found to substitute for ATP in the autoinactivation reaction of ...


    pub_type: 杂志文章


    authors: Maurizi MR,Ginsburg A

    更新日期:1986-01-14 00:00:00

  • Effects of the DNA intercalators 4'-(9-acridinylamino)methanesulfon-m-anisidide and 2-methyl-9-hydroxyellipticinium on topoisomerase II mediated DNA strand cleavage and strand passage.

    abstract::DNA topoisomerase II is believed to be the enzyme that produces the protein-associated DNA strand breaks observed in mammalian cell nuclei treated with various intercalating agents. Two intercalators--4'-(9-acridinylamino)methanesulfon-m-anisidide (m-AMSA, amsacrine) and 2-methyl-9-hydroxyellipticinium (2-Me-9-OH-E+)-...


    pub_type: 杂志文章


    authors: Pommier Y,Minford JK,Schwartz RE,Zwelling LA,Kohn KW

    更新日期:1985-11-05 00:00:00

  • Study of the spin-spin interactions between the metal centers of Desulfovibrio gigas aldehyde oxidoreductase: identification of the reducible sites of the [2Fe-2S]1+,2+ clusters.

    abstract::The aldehyde oxidoreductase from Desulfovibrio gigas belongs to the family of molybdenum hydroxylases. Besides a molybdenum cofactor which constitutes their active site, these enzymes contain two [2Fe-2S](2+,1+) clusters which are believed to transfer the electrons provided by the substrate to an acceptor which is eit...


    pub_type: 杂志文章


    authors: More C,Asso M,Roger G,Guigliarelli B,Caldeira J,Moura J,Bertrand P

    更新日期:2005-08-30 00:00:00

  • Design and chemical synthesis of a magnetic resonance contrast agent with enhanced in vitro binding, high blood-brain barrier permeability, and in vivo targeting to Alzheimer's disease amyloid plaques.

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    pub_type: 杂志文章


    authors: Poduslo JF,Curran GL,Peterson JA,McCormick DJ,Fauq AH,Khan MA,Wengenack TM

    更新日期:2004-05-25 00:00:00

  • Functional interactions in cytochrome P450BM3. Evidence that NADP(H) binding controls redox potentials of the flavin cofactors.

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    pub_type: 杂志文章


    authors: Murataliev MB,Feyereisen R

    更新日期:2000-10-17 00:00:00