Direct Interaction between the β-Amyloid Core and Tau Facilitates Cross-Seeding: A Novel Target for Therapeutic Intervention.

abstract：:A method for the rapid and quantitative analysis of 5'-terminal oligonucleotides of RNAs made in vitro is described. The method involves synthesis of RNA in the presence of [gamma-32P]ATP or GTP, isolation of the RNA, and digestion with T1 or pancreatic ribonucleases to release labeled 5'-triphosphate termanated oligo...

journal_title：Biochemistry

authors： Miller JS,Burgess RR

更新日期：1978-05-30 00:00:00

abstract：:Exogenous mannosylphosphoryldolichol (Man-P-Dol) has previously been shown to stimulate UDP-GlcNAc:dolichyl phosphate N-acetylglucosamine 1-phosphate transferase (GPT1), the enzyme catalyzing the biosynthesis of N-acetylglucosaminylpyrophosphoryldolichol (GlcNAc-P-P-Dol). To define the structural specificity of the ma...

journal_title：Biochemistry

authors： Kean EL,Rush JS,Waechter CJ

更新日期：1994-08-30 00:00:00

abstract：:The fatty acid acylated insulin, Lys(B29)-tetradecanoyl, des-(B30) human insulin, has been crystallized and the structure determined by X-ray crystallography. The fatty acid substituent on residue B29 Lys binds reversibly to circulating albumin protein in vivo, and by this mechanism the hormone's action is prolonged. ...

journal_title：Biochemistry

authors： Whittingham JL,Havelund S,Jonassen I

更新日期：1997-03-11 00:00:00

abstract：:Mutation of Pro94 to phenylalanine or alanine significantly alters the redox properties of the type I copper center of amicyanin. Each mutation increases the redox midpoint potential (E(m)) value by at least 140 mV and shifts the pK(a) for the pH dependence of the E(m) value to a more acidic value. Atomic resolution (...

journal_title：Biochemistry

authors： Carrell CJ,Sun D,Jiang S,Davidson VL,Mathews FS

更新日期：2004-07-27 00:00:00

abstract：:The insulin hexamer is an allosteric protein which displays positive and negative cooperativity and half-site reactivity that is modulated by strong homotropic and heterotropic ligand binding interactions at two different loci. These loci consist of phenolic pockets situated on the dimer-dimer interfaces of T-R and R-...

journal_title：Biochemistry

authors： Huang ST,Choi WE,Bloom C,Leuenberger M,Dunn MF

更新日期：1997-08-12 00:00:00

abstract：:Cnu is a nucleoid protein that has a high degree of sequence homology with Hha/YmoA family proteins, which bind to chromatin and regulate the expression of Escherichia coli virulence genes in response to changes in temperature or ionic strength. Here, we determined its solution structure and dynamic properties and map...

journal_title：Biochemistry

authors： Bae SH,Liu D,Lim HM,Lee Y,Choi BS

更新日期：2008-02-19 00:00:00

abstract：:Glutamate mutase is one of a group of adenosylcobalamin-dependent enzymes that catalyze unusual isomerizations that proceed through organic radical intermediates generated by homolytic fission of the coenzyme's unique cobalt-carbon bond. These enzymes are part of a larger family of enzymes that catalyze radical chemis...

journal_title：Biochemistry

authors： Cheng MC,Marsh EN

更新日期：2007-01-23 00:00:00

abstract：:Transcarboxylase (TC) is a multisubunit enzyme that catalyzes the transfer of a carboxylate group from methylmalonyl-CoA (MMCoA) to pyruvate. The CO2- group is shuttled between the MMCoA and pyruvate binding sites by a biotin cofactor, covalently linked to the 1.3S subunit. Fully carboxylated 1.3S can be prepared in v...

journal_title：Biochemistry

authors： Rivera-Hainaj RE,Pusztai-Carey M,Venkat Reddy D,Choowongkomon K,Sönnichsen FD,Carey PR

更新日期：2002-02-19 00:00:00

abstract：:Two transcription factors, CRP and CytR, mediate positive and negative control of nine cistrons involved in nucleoside catabolism and recycling in Escherichia coli. The ability of multiple transcription factors to combine in different ways to confer differential gene regulation is of significant interest in both proka...

journal_title：Biochemistry

authors： Holt AK,Senear DF

更新日期：2010-01-26 00:00:00

abstract：:Our earlier NMR study showed that a two-disulfide variant of hen lysozyme containing intra-alpha-domain disulfide bridges, C6-C127 and C30-C115, is partially folded, with the alpha domain tightly folded to the nativelike conformation and the beta domain flexible or unfolded. With a view that the formation of a third d...

journal_title：Biochemistry

authors： Yokota A,Hirai K,Miyauchi H,Iimura S,Noda Y,Inoue K,Akasaka K,Tachibana H,Segawa S

更新日期：2004-06-01 00:00:00

abstract：:Cyanobacteriochromes (CBCRs) are photosensory proteins related to the red/far-red phytochromes. Like phytochromes, CBCRs use linear tetrapyrrole (bilin) chromophores covalently attached via a thioether linkage to a conserved Cys residue also found in plant and cyanobacterial phytochromes. Unlike almost all phytochrome...

journal_title：Biochemistry

authors： Rockwell NC,Martin SS,Lagarias JC

更新日期：2012-05-01 00:00:00

abstract：:Glutamate semialdehyde aminomutase is a recognized target for selective herbicides and antibacterial agents because it provides the aminolevulinate from which tetrapyrroles are synthesized in plants and bacteria but not in animals. The reactions of the enzyme with R- and S-enantiomers of a novel compound, diaminopropy...

journal_title：Biochemistry

authors： Contestabile R,Jenn T,Akhtar M,Gani D,John RA

更新日期：2000-03-21 00:00:00

abstract：:TcmO is an O-methyltransferase that methylates the C-8 hydroxyl to Tcm B3, a four-ring aromatic intermediate in the tetracenomycin biosynthetic pathway of Streptomyces glaucescens. The gene encoding this enzyme was expressed in Streptomyces coelicolor CH999 together with the actinorhodin polyketide synthase (PKS) gene...

journal_title：Biochemistry

authors： Fu H,Alvarez MA,Khosla C,Bailey JE

更新日期：1996-05-28 00:00:00

abstract：:Several diseases, including Alzheimer's disease, Parkinson's disease, and Huntington's disease (HD), are associated with specific proteins aggregating and depositing within tissues and/or cellular compartments. The aggregation of these proteins is characterized by the formation of extended, β-sheet rich fibrils, terme...

journal_title：Biochemistry

authors： Beasley M,Stonebraker AR,Hasan I,Kapp KL,Liang BJ,Agarwal G,Groover S,Sedighi F,Legleiter J

更新日期：2019-10-29 00:00:00

abstract：:A method is described for using very high specific activity [3H]poly(deoxythymidylate) [[3H]poly(dT)] to detect, size, and quantiate subnanogram amounts of nonradioactive polyadenylated RNA. Short (approximately 100 nucleotides long) [3H]poly(dT) is hybridized to the poly(adenylate) [poly(A)] tracts in polyadenylated ...

journal_title：Biochemistry

authors： Tracy S,Kohne DE

更新日期：1980-08-05 00:00:00

abstract：:Difference Fourier transform infrared spectra of lumirhodopsin, metarhodopsin I, and metarhodopsin II versus rhodopsin were recorded with hydrated films of bovine rod outer segments at 200, 240, and 270 K. In the region between 3700 and 3450 cm-1, the O-H stretching vibrational bands of water were identified by H(2)18...

journal_title：Biochemistry

authors： Maeda A,Ohkita YJ,Sasaki J,Shichida Y,Yoshizawa T

更新日期：1993-11-16 00:00:00

abstract：:Pyrroloquinoline quinone (PQQ) is a small, redox active molecule that serves as a cofactor for several bacterial dehydrogenases, introducing pathways for carbon utilization that confer a growth advantage. Early studies had implicated a ribosomally translated peptide as the substrate for PQQ production. This study pres...

journal_title：Biochemistry

authors： Shen YQ,Bonnot F,Imsand EM,RoseFigura JM,Sjölander K,Klinman JP

更新日期：2012-03-20 00:00:00

abstract：:The transfer of ganglioside GM1 from micelles to membranes and between different membrane populations has been examined by using a pyrene fatty acid derivative of the ganglioside. The transfer of gangliosides from micelles to membranes depends on the physical state as well as the molecular composition of the acceptor ...

journal_title：Biochemistry

authors： Masserini M,Freire E

更新日期：1987-01-13 00:00:00

abstract：:Terreic acid is a metabolite with antibiotic properties produced by the fungus Aspergillus terreus. We found that terreic acid is a covalent inhibitor of the bacterial cell wall biosynthetic enzyme MurA from Enterobacter cloacae and Escherichia coli in vitro. The crystal structure of the MurA dead-end complex with ter...

journal_title：Biochemistry

authors： Han H,Yang Y,Olesen SH,Becker A,Betzi S,Schönbrunn E

更新日期：2010-05-18 00:00:00

abstract：:The role of ATP hydrolysis by the 44/62 protein in formation of the stable holoenzyme DNA replication complex has been further elucidated by specifically examining the role that the 44/62 protein plays in loading the 45 protein onto the DNA substrate. A stable phospho-45 protein or phosphorylated holoenzyme complex wa...

journal_title：Biochemistry

authors： Berdis AJ,Benkovic SJ

更新日期：1997-03-11 00:00:00

abstract：:The nuclease hypersensitivity element III(1) (NHE III(1)) of the c-MYC promoter strongly controls the transcriptional activity of the c-MYC oncogene. The purine-rich strand of the NHE III(1) element has been shown to be a silencer element for c-MYC transcription upon formation of a G-quadruplex structure. We have dete...

journal_title：Biochemistry

authors： Ambrus A,Chen D,Dai J,Jones RA,Yang D

更新日期：2005-02-15 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae, CTP synthetase [EC 6.3.4.2; UTP:ammonia ligase (ADP-forming)] is the product of the URA7 gene. CTP synthetase was purified 503-fold to apparent homogeneity from cells bearing the URA7 gene on a multicopy plasmid that directed a 10-fold overproduction of the enzyme. The purificati...

journal_title：Biochemistry

authors： Yang WL,McDonough VM,Ozier-Kalogeropoulos O,Adeline MT,Flocco MT,Carman GM

更新日期：1994-09-06 00:00:00

abstract：:Detailed analysis of factors governing high affinity antibody-antigen interactions yields important insight into molecular recognition and facilitates the design of functional antibody libraries. Here we describe comprehensive mutagenesis of the light chain complementarity determining regions (CDRs) of HIV-1 antibody ...

journal_title：Biochemistry

authors： Da Silva GF,Harrison JS,Lai JR

更新日期：2010-07-06 00:00:00

abstract：:The turnover of the collagen triple-helical structure (collagenolysis) is a tightly regulated process in normal physiology and has been ascribed to a small number of proteases. Several members of the matrix metalloproteinase (MMPs) family possess collagenolytic activity, and the mechanisms by which these enzymes proce...

journal_title：Biochemistry

authors： Minond D,Lauer-Fields JL,Cudic M,Overall CM,Pei D,Brew K,Moss ML,Fields GB

更新日期：2007-03-27 00:00:00

abstract：:The association of membrane and cytoplasmic proteins with the cytoskeleton of resting and activated platelets was studied. Glycoproteins were identified by labeling with 125I-labeled lectins (concanavalin A, wheat germ agglutinin, and Lens culinaris). Polypeptides, which are embedded in the lipid bilayer, have been id...

journal_title：Biochemistry

authors： Rotman A,Heldman J,Linder S

更新日期：1982-04-13 00:00:00

abstract：:Nitric oxide is produced in mammals by the nitric oxide synthase (NOS) isoforms at a catalytic site comprising a heme associated with a biopterin cofactor. Through genome sequencing, proteins that are highly homologous to the oxygenase domain of NOSs have been identified, in particular in bacteria. The active site is ...

journal_title：Biochemistry

authors： Weisslocker-Schaetzel M,Lembrouk M,Santolini J,Dorlet P

更新日期：2017-02-07 00:00:00

abstract：:The (1)H NMR resonances of the heme substituents of the low-spin Fe(III) form of nitrophorin 2, as its complexes with N-methylimidazole (NP2-NMeIm) and imidazole (NP2-ImH), have been assigned by a combination of (1)H homonuclear two-dimensional NMR techniques and (1)H-(13)C HMQC. Complete assignment of the proton and ...

journal_title：Biochemistry

authors： Shokhireva TKh,Shokhirev NV,Walker FA

更新日期：2003-01-28 00:00:00

abstract：:Thermodynamic studies of bacteriorhodopsin (BR) have been undertaken in order to investigate the factors that stabilize the structure of a membrane protein. The stability of the native, intact protein was compared to that of protein with retinal removed, and/or cleaved in one or two of the loops connecting the transme...

journal_title：Biochemistry

authors： Kahn TW,Sturtevant JM,Engelman DM

更新日期：1992-09-22 00:00:00

abstract：:The association-dissociation kinetics of ribosomes from Escherichia coli have been studied under various conditions in a light-scattering stopped-flow apparatus. The dissociation reaction at 2 mg/ml at 25 degrees C, induced by lowering the MgCl2 concentration from 18 to 3 mM, can best be described by three independent...

journal_title：Biochemistry

authors： Görisch H,Goss DJ,Parkhurst LJ

更新日期：1976-12-28 00:00:00

abstract：:Seven hydrophobic residues ranging in size from glycine to phenylalanine have been substituted for the wild-type methionine residue at position 13 in a 15-residue truncated version (S15) of S-peptide, the small component of ribonuclease S. Complexes of both S-15 and the seven variants with S-protein yielded isomorphou...

journal_title：Biochemistry

authors： Varadarajan R,Richards FM

更新日期：1992-12-15 00:00:00