Abstract:
:Difference Fourier transform infrared spectra of lumirhodopsin, metarhodopsin I, and metarhodopsin II versus rhodopsin were recorded with hydrated films of bovine rod outer segments at 200, 240, and 270 K. In the region between 3700 and 3450 cm-1, the O-H stretching vibrational bands of water were identified by H(2)18O and 2H2O shifts. Lumirhodopsin and metarhodopsin I exhibit almost identical spectral shape in this region. The O-H stretching vibration band of water was detected at 3533 cm-1 upon formation of lumirhodopsin and metarhodopsin I and at 3641 cm-1 upon formation of metarhodopsin II. The results suggest that hydrogen bonding of water molecules in the protein is stronger in lumirhodopsin and metarhodopsin I, intermediates with a protonated Schiff base, than in metarhodopsin II with an unprotonated Schiff base. This is similar to the case of photoreaction of bacteriorhodopsin, in which stronger hydrogen bonding of water is formed in the L intermediate than the M intermediate [Maeda, A., Sasaki, J., Shichida, Y., & Yoshizawa, T. (1992) Biochemistry 31, 462-467].
journal_name
Biochemistryjournal_title
Biochemistryauthors
Maeda A,Ohkita YJ,Sasaki J,Shichida Y,Yoshizawa Tdoi
10.1021/bi00096a013subject
Has Abstractpub_date
1993-11-16 00:00:00pages
12033-8issue
45eissn
0006-2960issn
1520-4995journal_volume
32pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Steroid receptors define a family of ligand-activated transcription factors. Recent work has demonstrated that the receptors regulate distinct but overlapping gene networks, yet the mechanisms by which they do so remain unclear. We previously determined the microscopic binding energetics for progesterone receptor (PR)...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi2017156
更新日期:2012-01-24 00:00:00
abstract::A modified multiple binding equilibria treatment is presented that allows determination of thermodynamic parameters of the interaction of phospholipids with integral membrane proteins solubilized in excess detergent. Lipid binding is modeled as a series of exchange reactions between lipid molecules and detergent molec...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00376a019
更新日期:1987-01-27 00:00:00
abstract::The nitrogenase MoFe protein contains the active site metallocluster called FeMo-cofactor [7Fe-9S-Mo-homocitrate] that exhibits an S = 3/2 EPR signal in the resting state. No interaction with FeMo-cofactor is detected when either substrates or inhibitors are incubated with MoFe protein in the resting state. Rather, th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011571m
更新日期:2001-11-20 00:00:00
abstract::The retaining beta-1,4-glycanase Cex from Cellulomonas fimi, a family 10 glycosyl hydrolase, hydrolyzes xylan 40-fold more efficiently than cellulose. To gain insight into the nature of its preference for xylan, we determined the crystal structure of the Cex catalytic domain (Cex-cd) trapped as its covalent 2-deoxy-2-...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9729211
更新日期:1998-04-07 00:00:00
abstract::A single histidine residue has been placed at either the N-terminus or the C-terminus of each of the two alpha-helices of barnase. The pKa of that histidine residue in each of the four mutants has been determined by 1H NMR. The pKas of the two residues at the C-terminus are, on average, 0.5 unit higher, and those of t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00123a006
更新日期:1992-03-03 00:00:00
abstract::The kringle 2 (K2) module of human plasminogen (Pgn) binds L-lysine and analogous zwitterionic compounds, such as the antifibronolytic agent trans-(aminomethyl)cyclohexanecarboxylic acid (AMCHA). Far-UV CD and NMR spectra reveal little conformational change in K2 upon ligand binding. However, retarded (1)H-(2)H isotop...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9917378
更新日期:1999-11-30 00:00:00
abstract::(E)-4-Hydroxy-3-methylbut-2-enyl diphosphate reductase (IspH or LytB) catalyzes the terminal step of the MEP/DOXP pathway where it converts (E)-4-hydroxy-3-methylbut-2-enyl diphosphate (HMBPP) into the two products, isopentenyl diphosphate and dimethylallyl diphosphate. The reaction involves the reductive elimination ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi3001215
更新日期:2012-06-19 00:00:00
abstract::Serine palmitoyltransferase (EC 2.3.1.50) catalyzes the condensation of L-serine and palmitoyl-CoA to yield 3-ketosphinganine in the first unique reaction of long-chain (sphingoid) base biosynthesis. The kinetic effects of changing the extracellular concentrations of the precursors for this pathway were studied with L...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00401a051
更新日期:1988-01-12 00:00:00
abstract::The pH dependence of the 13C chemical shifts of the side-chain carboxyl carbons of all Asp and Glu residues in the reduced and oxidized states of human thioredoxin and in a mixed disulfide complex of human thioredoxin with a target peptide from the transcription factor NF kappa B has been investigated by multidimensio...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952299h
更新日期:1996-01-09 00:00:00
abstract::The zinc metalloenzyme protein farnesyltransferase (FTase) catalyzes the alkylation of a cysteine residue of protein substrates with a 15 carbon farnesyl group. We have developed fluorescence assays to directly measure the affinity of the enzyme for peptide and protein (Ras) substrates. A peptide corresponding to the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981525v
更新日期:1998-11-03 00:00:00
abstract::Several 3-oxo-4-aza steroids (1) have been identified as inhibitors of the 3 beta-hydroxy-delta 5-steroid dehydrogenase/3-keto-delta 5-steroid isomerase catalyzed conversion of pregnenolone to progesterone. By kinetically decoupling the two enzyme activities isolated from bovine adrenal cortex, it has been demonstrate...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00427a021
更新日期:1989-01-10 00:00:00
abstract::Insulin-stimulated glucose disposal in skeletal muscle proceeds predominantly through a nonoxidative pathway with glycogen synthase as a rate-limiting enzyme, yet the mechanisms for insulin activation of glycogen synthase are not understood despite years of investigation. Isolation of putative insulin second messenger...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0508845
更新日期:2005-08-23 00:00:00
abstract::It has been shown (Poole et al., 1991) that deletion of residues 44-49 from the sequence of staphylococcal nuclease (E43 SNase) results in an enzyme (E43 delta SNase) that is significantly more active than D43 SNase, an enzyme that differs from the wild-type enzyme by deletion of a single methylene group. In addition,...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00229a006
更新日期:1991-04-16 00:00:00
abstract::The potassium form of d(CGCGAATTCGCG) solved by X-ray diffraction to 1.75 A resolution indicates that monovalent cations penetrate the primary and secondary layers of the "spine of hydration". Both the sodium [Shui, X., McFail-Isom, L., Hu, G. G., and Williams, L. D. (1998) Biochemistry 37, 8341-8355] and the potassiu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi982063o
更新日期:1998-12-01 00:00:00
abstract::Living organisms have evolved a vast array of catalytic functions that make them ideally suited for the production of medicinally and industrially relevant small-molecule targets. Indeed, native metabolic pathways in microbial hosts have long been exploited and optimized for the scalable production of both fine and co...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/bi200416g
更新日期:2011-06-21 00:00:00
abstract::The photobleaching pathway of a short-wavelength cone opsin purified in delipidated form (lambda(max) = 425 nm) is reported. The batho intermediate of the violet cone opsin generated at 45 K has an absorption maximum at 450 nm. The batho intermediate thermally decays to the lumi intermediate (lambda(max) = 435 nm) at ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi010387y
更新日期:2001-07-03 00:00:00
abstract::Two forms of cytochrome P-450, designated P-450NMa and P-450NMb, were purified to electrophoretic homogeneity from rabbit nasal microsomes. The purified cytochromes, which contained 14-16 nmol of P-450/mg of protein, exhibited apparent monomeric molecular weights of 49,500 and 51,000, respectively. As indicated by sev...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00422a007
更新日期:1988-11-01 00:00:00
abstract::The interaction of bleomycin A2 with DNA has been examined by fluorescence spectroscopy and proton magnetic resonance techniques. Fluorescence bands observed at 353 and 405 nm in the spectrum of bleomycin were assigned to the bithiazole and 4-aminopyrimidine rings, respectively. Quenching of bithiazole fluorescence by...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00635a021
更新日期:1977-08-09 00:00:00
abstract::Thromboxane A(2) receptor (TP receptor), a prostanoid receptor, belongs to the G protein-coupled receptor family, composed of three intracellular loops and three extracellular loops connecting seven transmembrane helices. The highly conserved extracellular domains of the prostanoid receptors were found in the second e...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001867c
更新日期:2001-01-09 00:00:00
abstract::Merozoite surface protein 2 (MSP2), an abundant glycosylphosphatidylinositol-anchored protein on the surface of Plasmodium falciparum merozoites, is a promising malaria vaccine candidate. MSP2 is intrinsically disordered and forms amyloid-like fibrils in solution under physiological conditions. The 25 N-terminal resid...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi201880s
更新日期:2012-02-21 00:00:00
abstract::The substrate specificity of the human enzyme glutathione reductase was changed from its natural substrate glutathione to trypanothione [N1,N8-bis(glutathionyl)spermidine] by site-directed mutagenesis of two residues. The glutathione analogue, trypanothione, is the natural substrate for trypanothione reductase, an enz...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00239a006
更新日期:1991-06-25 00:00:00
abstract::Ultraviolet irradiation of calmodulin in the presence of calcium results in either the intramolecular cross-linking of Tyr99 and Tyr138 [Malencik, D.A., & Anderson, S.R. (1986) Biochemistry 25, 709] or, when [Tyr8]substance P is bound, the generation of peptide-calmodulin adducts. The latter consist of two chromatogra...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00403a016
更新日期:1988-02-09 00:00:00
abstract::In the commonly used nucleation-dependent model of protein aggregation, aggregation proceeds only after a lag phase in which the concentration of energetically unfavorable nuclei reaches a critical value. The formation of oligomeric species prior to aggregation can be difficult to detect by current spectroscopic techn...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi400027y
更新日期:2013-03-19 00:00:00
abstract::Human NFU (also known as HIRIP5) has been implicated in cellular iron-sulfur cluster biosynthesis. Bacterial and yeast forms are smaller than the human protein and are homologous to the C-terminal domain of the latter. This C-terminal domain contains a pair of redox active cysteines and demonstrates thioredoxin-like a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801645z
更新日期:2009-02-10 00:00:00
abstract::Thermodynamic parameters of helix formation were measured spectroscopically for 16 oligoribonucleotides containing either internal GU mismatches or the corresponding AU pairs. Internal GU mismatches stabilize each helix, but not as much as the corresponding AU pairs. The differences in the enthalpy and entropy changes...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00367a061
更新日期:1986-09-23 00:00:00
abstract::Strong inward-rectifier K(+) (Kir) channels play a significant role in shaping the cardiac action potential: they help produce its long plateau and accelerate its rate of repolarization. Consequently, genetic deletion of the gene encoding the strongly rectifying K(+) channel IRK1 (Kir2.1) prolongs the cardiac action p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0483099
更新日期:2004-12-14 00:00:00
abstract::The nuclear tRNA 3' processing activity from wheat has been characterized and partially purified. Several characteristics of the wheat nuclear 3' processing enzyme now allow this activity to be distinguished from its mitochondrial counterpart. The nuclear enzyme is an endonuclease, which we termed nuclear RNase Z. The...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi992253e
更新日期:2000-02-29 00:00:00
abstract::Kinetic studies of reactions between fluorescein and antifluorescein antibody produced during early, intermediate, and late stages of the immune response have been carried out utilizing both fluorescence intensity and polarization measurements in the static (time constant similar to 5 sec) and in the stopped-flow mode...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00688a009
更新日期:1975-08-26 00:00:00
abstract::PDZ (acronym of the synapse-associated protein PSD-95/SAP90, the septate junction protein Discs-large, and the tight junction protein ZO-1) domains are abundant small globular protein interaction domains that mainly recognize the carboxyl termini of their target proteins. Detailed knowledge on PDZ domain binding speci...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700954e
更新日期:2007-11-27 00:00:00
abstract::The capsular polysaccharide (CPS) structure of Campylobacter jejuni contributes to its robust fitness. Many strains contain heptose moieties in their CPS units. The precursor heptose is GDP-d-glycero-α-d-manno-heptose; modifications to the stereochemistry at C3-C6 as well as additions of methyl and phosphoramidate gro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b00548
更新日期:2019-09-17 00:00:00