Abstract:
:Peroxynitrite-mediated oxidative chemistry is currently the subject of intense investigation owing to the toxic side effects associated with nitric oxide overproduction. Using direct electron spin resonance spectroscopy (ESR) at 37 degrees C, we observed that in human erythrocytes peroxynitrite induced a long-lived singlet signal at g = 2.004 arising from hemoglobin. This signal was detectable in oxygenated red blood cells and in purified oxyhemoglobin but significantly decreased after deoxygenation. The formation of the g = 2.004 radical required the presence of CO2 and pH values higher than the pKa of peroxynitrous acid (pKa = 6.8), indicating the involvement of a secondary oxidant formed in the interaction of ONOO- with CO2. The g = 2.004 radical yield leveled off at a 1:1 ratio between peroxynitrite and oxyhemoglobin, while CO-hemoglobin formed less radical and methemoglobin did not form the radical at all. These results suggest that the actual oxidant is or is derived from the ONOOCO2- adduct interacting with oxygenated FeII-heme. Spin trapping with 2-methyl-2-nitrosopropane (MNP) of the g = 2.004 radical and subsequent proteolytic digestion of the MNP/hemoglobin adduct revealed the trapping of a tyrosyl-centered radical(s). A similar long-lived unresolved g = 2.004 singlet signal is a common feature of methemoglobin/H2O2 and metmyoglobin/H2O2 systems. We show by spin trapping that these g = 2.004 signals generated by H2O2 also indicated trapping of radicals centered on tyrosine residues. Analysis of visible spectra of hemoglobin treated with peroxynitrite revealed that, in the presence of CO2, oxyhemoglobin was oxidized to a ferryl species, which rapidly decayed to lower iron oxidation states. The g = 2.004 radical may be an intermediate formed during ferrylhemoglobin decay. Our results describe a new pathway of peroxynitrite-dependent hemoglobin oxidation of dominating importance in CO2-containing biological systems and identify the g = 2.004 radical(s) formed in the process as tyrosyl radical(s).
journal_name
Biochemistryjournal_title
Biochemistryauthors
Minetti M,Scorza G,Pietraforte Ddoi
10.1021/bi982311gsubject
Has Abstractpub_date
1999-02-16 00:00:00pages
2078-87issue
7eissn
0006-2960issn
1520-4995pii
bi982311gjournal_volume
38pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Heme-copper oxidases are membrane-bound proteins that catalyze the reduction of O(2) to H(2)O, a highly exergonic reaction. Part of the free energy of this reaction is used for pumping of protons across the membrane. The ba(3) oxidase from Thermus thermophilus presumably uses a single proton pathway for the transfer o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300132t
更新日期:2012-06-05 00:00:00
abstract::Sorcin, a 21.6 kDa cytosolic EF-hand protein which undergoes a Ca(2+)-induced translocation from cytoplasm to membranes, has been assigned to the newly defined penta EF-hand family. A molecular model of the C-terminal Ca(2+)-binding domain has been generated using as a template the X-ray coordinates of the correspondi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi991648v
更新日期:2000-02-01 00:00:00
abstract::The Fo membrane domain of the F1Fo-ATP synthase complex has been purified from bovine heart mitochondria. The purification procedure involves the removal of peripheral membrane proteins, including F1-ATPase, from submitochondrial particles with guanidine hydrochloride, followed by extraction of Fo and other membrane p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00191a026
更新日期:1994-06-28 00:00:00
abstract::We have determined the mechanism of DNA-dependent protein kinase (DNA-PK) inhibition by cis-diamminedichloroplatinum(II)-(cisplatin-) damaged DNA. We previously have demonstrated that Ku, the DNA binding subunit of DNA-PK, is capable of binding to DNA duplexes globally damaged with cisplatin but was unable to stimulat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi963124q
更新日期:1997-06-17 00:00:00
abstract::Binding of 125I-labeled human alpha-thrombin to endothelial cells derived from bovine corneas was studied in tissue culture. Specific and saturable binding to the cell surface occurred at 37 degrees C but to a much smaller extent at 4 degrees C. Binding of [125I]thrombin to a specific site on these cells with formatio...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00505a027
更新日期:1981-01-20 00:00:00
abstract::Doxorubicin (DOX) is a chemotherapeutic that is used in the treatment of a wide variety of cancers. However, it causes cardiotoxicity partly because of the formation of reactive oxygen species. CYP2J2 is a human cytochrome P450 that is strongly expressed in cardiomyocytes. It converts arachidonic acid (AA) into four d...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b01025
更新日期:2017-12-26 00:00:00
abstract::The structural factors responsible for the extraordinary rate enhancement ( approximately 10(17)) of the reaction catalyzed by orotidine 5'-monophosphate decarboxylase (OMPDC) have not been defined. Catalysis requires a conformational change that closes an active site loop and "clamps" the orotate base proximal to hyd...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi100443a
更新日期:2010-05-04 00:00:00
abstract::Putative domain--domain interactions of the monomeric bifunctional enzyme indoleglycerol phosphate synthase:phosphoribosyl anthranilate isomerase from Escherichia coli were probed by separating the domains on the gene level and expressing them as monofunctional proteins. The engineered monofunctional enzymes were foun...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00016a013
更新日期:1995-04-25 00:00:00
abstract::Animal galectins (lectins with specificity for beta-galactosides of glycan chains) are potent effectors in diverse aspects of cell sociology. Gene divergence has led to different groups and a marked interspecies variability in the number of members per group. Since the suitability of a model for studying functionality...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900083q
更新日期:2009-05-26 00:00:00
abstract::Retinoid X receptors (RXRs) are transcription factors with important functions in embryonic development, metabolic processes, differentiation, and apoptosis. A particular feature of RXRs is their ability to act as obligatory heterodimerization partners of class II nuclear receptors. At the same time, these receptors a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b01122
更新日期:2016-03-29 00:00:00
abstract::More than 100 distinct mutations in the gene CuZnSOD encoding human copper-zinc superoxide dismutase (CuZnSOD) have been associated with familial amyotrophic lateral sclerosis (fALS), a fatal neuronal disease. Many studies of different mutant proteins have found effects on protein stability, catalytic activity, and me...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500606j
更新日期:2015-01-20 00:00:00
abstract::Glutamate151 (E151) has been shown to be catalytically essential for the aminopeptidase from Vibrio proteolyticus (AAP). E151 acts as the general acid/base during the catalytic mechanism of peptide hydrolysis. However, a glutamate residue is not the only residue capable of functioning as a general acid/base during cat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0505823
更新日期:2005-09-13 00:00:00
abstract::The PufX membrane protein is essential for photosynthetic growth of Rhodobacter sphaeroides because it is required for multiple-turnover electron transfer under anaerobic conditions [see accompanying article; Barz, W. P., Francia, F., Venturoli, G., Melandri, B. A., Verméglio, A., & Oesterhelt, D. (1995) Biochemistry ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00046a033
更新日期:1995-11-21 00:00:00
abstract::Known crystal structures of class II aminoacyl-tRNA synthetases complexed to their cognate tRNAs reveal that critical acceptor stem contacts are made by the variable loop connecting the beta-strands of motif 2 located within the catalytic core of class II synthetases. To identify potential acceptor stem contacts made ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001835p
更新日期:2000-12-19 00:00:00
abstract::Rabbit lung flavin-containing monooxygenase (FMO, EC 1.14.13.8) was denatured, reduced, carboxymethylated, digested with endoproteinase Glu-C or trypsin, and subjected to mass spectrometric analysis. The amino acid sequences of selected peptides were determined by tandem mass spectrometry. Over 90% of rabbit lung FMO ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00105a012
更新日期:1991-10-15 00:00:00
abstract::Human chorionic gonadotropin (hCG) is a heterodimeric glycoprotein hormone involved in the maintenance of the corpus luteum in early pregnancy. The free alpha-subunit of hCG has a biological activity of its own, namely, stimulation of prolactin secretion from term pregnancy decidual cells [Blithe, D. L., et al. (1991)...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9718548
更新日期:1998-02-17 00:00:00
abstract::Although plasma membrane domains, such as caveolae, provide an organizing principle for signaling pathways and cholesterol homeostasis in the cell, relatively little is known regarding specific mechanisms, whereby intracellular lipid-binding proteins are targeted to caveolae. Therefore, the interaction between caveoli...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi7002636
更新日期:2007-07-17 00:00:00
abstract::Glutamate racemase, MurI, catalyzes the interconversion of glutamate enantiomers in a cofactor-independent fashion and provides bacteria with a source of D-Glu for use in peptidoglycan biosynthesis. The enzyme uses a "two-base" mechanism involving a deprotonation of the substrate at the alpha-position to form an anion...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi002703z
更新日期:2001-05-29 00:00:00
abstract::Structural changes in the complex formation between transducin and metarhodopsin II, the activated form of photolyzed rhodopsin, in visual transduction processes were analyzed by Fourier transform infrared spectroscopy. The spectrum of the complex was obtained by subtracting the contribution of metarhodopsin I and unc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi960911e
更新日期:1996-10-15 00:00:00
abstract::We have measured the frequency of the carbon-hydrogen stretching mode of the pro-R and pro-S C4-H bonds of NADH in solution and when bound to pig heart lactate (LDH) or mitochondrial malate (mMDH) dehydrogenases. This is achieved by specifically deuterating the C4 pro-R or pro-S hydrogens of NADH and determining the f...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00136a022
更新日期:1992-06-02 00:00:00
abstract::The maltodextrin binding protein from Escherichia coli serves as the initial receptor for both the active transport of and chemotaxis toward a range of linear maltose sugars. The X-ray structures of both the maltose-bound and sugar-free forms of the protein have been previously described [Spurlino, J. C., Lu, G.-Y., &...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00091a004
更新日期:1993-10-12 00:00:00
abstract::Synthetic ligands containing up to six residues of nonreducing terminal galactose were prepared. The synthesis involved coupling of carboxyl groups of N-benzyloxy-carbonylaspartic acid or of N-benzyloxycarbonyltyrosyl-gamma-glutamylglutamic acid to the omega-amino group of the aglycon of a glycoside that contained up ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00313a037
更新日期:1984-08-28 00:00:00
abstract::Holliday junctions are central intermediates in the process of genetic recombination. These DNA molecules contain four double helices that flank a central branch point, so that each of the four strands that constitute the junction is associated with two different helices. Previously, we have shown that such branched j...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00003a026
更新日期:1995-01-24 00:00:00
abstract::The actin depolymerizing factor (ADF)/cofilins are an essential group of proteins that are important regulators of actin filament turnover in vivo. Although protists and yeasts express only a single member of this family, metazoans express two or more members in many cell types. In cells expressing both ADF and cofili...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049797n
更新日期:2004-06-08 00:00:00
abstract::Inherently curved DNA segments, associated with short runs of adenines, have been identified in many gene regulatory regions, yet their physiological significance remains unknown. The observations reported in this study indicate that intrinsically bent nucleic acid fragments are characterized by substantially attenuat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00123a019
更新日期:1992-03-03 00:00:00
abstract::Intrinsic factor (IF(50)) is a cobalamin (Cbl)-transporting protein of 50 kDa, which can be cleaved into two fragments: the 30 kDa N-terminal peptide IF(30) and the 20 kDa C-terminal glycopeptide IF(20). Experiments on binding of Cbl to IF(30), IF(20), and IF(50) revealed comparable association rate constants (k(+)(Cb...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi047936v
更新日期:2005-03-08 00:00:00
abstract::Upon injury of a blood vessel, activated factor VII (FVIIa) forms a high-affinity complex with its allosteric regulator, tissue factor (TF), and initiates blood clotting. Active site-inhibited factor VIIa (FVIIai) binds to TF with even higher affinity. We compared the interactions of FVIIai and FVIIa with soluble TF (...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi010283n
更新日期:2001-08-07 00:00:00
abstract::The physicochemical and catalytic properties of thioredoxin-T' are described. This complemented protein structure consists of a 1:1 complex between the inactive fragments thioredoxin-T-(1--73) and thioredoxin T-(74--108). These are generated by selective trypsin cleavage at Arg-73 in lysine-modified and denatured Esch...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00592a010
更新日期:1979-12-11 00:00:00
abstract::In this work we report the synthesis of a radioactive, photolabile oligodeoxyribonucleotide probe and its exploitation in identifying 50S ribosomal subunit components neighboring its target site in 23S rRNA. The probe is complementary to 23S rRNA nucleotides 2497-2505, a single-stranded sequence that has been shown to...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00236a014
更新日期:1991-06-04 00:00:00
abstract::Under mildly acidic conditions, annexin 12 (ANX) inserts into lipid membranes to form a transbilayer pore [Langen, R., et al. (1998) Proc. Natl. Acad. Sci. U.S.A. 95, 14060]. In this study, we have addressed the question of the oligomeric state of ANX in this transbilayer conformation by means of Forster-type resonanc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi047805u
更新日期:2005-03-08 00:00:00