Abstract：
:We have measured the frequency of the carbon-hydrogen stretching mode of the pro-R and pro-S C4-H bonds of NADH in solution and when bound to pig heart lactate (LDH) or mitochondrial malate (mMDH) dehydrogenases. This is achieved by specifically deuterating the C4 pro-R or pro-S hydrogens of NADH and determining the frequencies of the resulting C4-D stretches by Raman difference spectroscopy. We find that the frequencies of the two C4-D stretching modes for the two bonds are essentially the same for the unliganded coenzyme. On the other hand, the position of the pro-S-[4-2H]NADH stretch shifts upward by about 23-30 cm-1 in its binary complex with either lactate or malate dehydrogenase relative to that observed in solution, while that for the bound pro-R-[4-2H]NADH is relatively unchanged. The fact that the frequency of the pro-R hydrogen is not significantly affected during complex formation suggests that the rate enhancements for reaction of substrate with NADH brought about by both pig heart LDH and mMDH apparently do not involve either stabilization or destabilization of the pro-R hydrogen of NADH in enzyme-coenzyme binary complexes, in agreement with previous chemical studies. That these proteins are able to regulate the frequencies of the two C4-D bonds differentially, and hence the electronic distributions in these bonds, has important implications for the stereochemical reactions catalyzed by the NAD dehydrogenases, and this is discussed.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
Deng H,Zheng J,Sloan D,Burgner J,Callender Rdoi
10.1021/bi00136a022subject
Has Abstractpub_date
1992-06-02 00:00:00pages
5085-92issue
21eissn
0006-2960issn
1520-4995journal_volume
31pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Picosecond absorption spectroscopy has been used to investigate energy-transfer dynamics within the LH1 and LH2 light-harvesting complexes of three mutants of Rhodobacter sphaeroides. We demonstrate that both complexes are inhomogeneous; each contains a specialized pigment pool which absorbs maximally at a longer wave...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00465a008
更新日期：1990-04-03 00:00:00
abstract：:NADH:ubiquinone oxidoreductase (complex I) from bovine heart mitochondria is a highly complicated, energy transducing, membrane-bound enzyme. It contains 46 different subunits and nine redox cofactors: a noncovalently bound flavin mononucleotide and eight iron-sulfur clusters. The mechanism of complex I is not known. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051809x
更新日期：2006-01-10 00:00:00
abstract：:Band 3, the anion transport protein of human erythrocyte membranes, can be transferred from cells to liposomes and from liposomes back to cell membranes, retaining function and native orientation. After incubation with cells, sonicated phosphatidylcholine vesicles bind a transmembrane protein that comigrates with band...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00295a011
更新日期：1983-12-20 00:00:00
abstract：:The in vitro hydrolysis by porcine kidney prolidase of the imidodipeptide L-alanyl-L-proline was monitored by using 1H high-resolution NMR spectroscopy. The dipeptide exists as an equilibrium mixture of isomers with cis or trans conformation about the peptide bond. The 13C and 1H NMR spectra of the dipeptide displayed...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00353a016
更新日期：1986-03-11 00:00:00
abstract：:FLI1 (Friend leukemia integration 1) is a metazoan transcription factor that is upregulated in a number of cancers. In addition, rearrangements of the fli1 gene cause sarcomas, leukemias, and lymphomas. These rearrangements encode oncogenic transcription factors, in which the DNA binding domain (DBD or ETS domain) of ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b01121
更新日期：2015-12-22 00:00:00
abstract：:A nucleoside triphosphate binding site on calf thymus RNA polymerase II was identified by using photoaffinity analogues of adenosine 5'-triphosphate and guanosine 5'-triphosphate. Both radiolabeled 8-azidoadenosine 5'-triphosphate (8-N3ATP) and radiolabeled 8-azidoguanosine 5'-triphosphate (8-N3GTP) bound to a single ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00349a038
更新日期：1986-01-14 00:00:00
abstract：:Apamin is being studied as a model for the folding mechanism of proteins whose structures are stabilized by disulfide bonds. Apamin consists of 18 amino acid residues and forms a stable structure consisting of a C-terminal alpha-helix and two reverse turns. This structure is stabilized by two disulfide bonds connectin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00133a009
更新日期：1992-05-12 00:00:00
abstract：:The side chains of R269 and N270 interact with the phosphodianion of dihydroxyacetone phosphate (DHAP) bound to glycerol 3-phosphate dehydrogenase (GPDH). The R269A, N270A, and R269A/N270A mutations of GPDH result in 9.1, 5.6, and 11.5 kcal/mol destabilization, respectively, of the transition state for GPDH-catalyzed ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00116
更新日期：2016-03-15 00:00:00
abstract：:Plasma kallikrein (Fletcher factor) is a hepatic serine proteinase that participated in the early phase of blood coagulation. From two genomic libraries, we succeeded to isolate four overlapping clones representing the entire rat plasma kallikrein gene. Using selective DNA sequencing, polymerase chain reactions, and r...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00220a027
更新日期：1991-02-12 00:00:00
abstract：:The envelope glycoprotein (G protein) of vesicular stomatitis virus probably exists in the viral envelope as a trimer of identical subunits. Depending on the conditions of solubilization, G protein may dissociate into monomers. G protein solubilized with the detergent octyl glucoside was shown to exist as oligomeric f...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00462a002
更新日期：1990-03-13 00:00:00
abstract：:The tertiary structure of alpha1-acid glycoprotein (AGP) remains unresolved despite its novel function because AGP is a hard target in X-ray and NMR analyses. To elucidate the membrane-induced conformational change of AGP, the vacuum-ultraviolet circular dichroism (VUVCD) spectra of AGP and its constituent sugars were...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901184r
更新日期：2009-09-29 00:00:00
abstract：:Two forms of cytochrome P-450, designated P-450NMa and P-450NMb, were purified to electrophoretic homogeneity from rabbit nasal microsomes. The purified cytochromes, which contained 14-16 nmol of P-450/mg of protein, exhibited apparent monomeric molecular weights of 49,500 and 51,000, respectively. As indicated by sev...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00422a007
更新日期：1988-11-01 00:00:00
abstract：:MreB is a bacterial orthologue of actin that affects cell shape, polarity, and chromosome segregation. Although a significant body of work has explored its cellular functions, we know very little about the biochemical behavior of MreB. We have cloned, overexpressed in Escherichia coli, and purified untagged MreB1 from...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi701538e
更新日期：2008-01-15 00:00:00
abstract：:Binding mechanisms of ADPR-transferase to restricted double-stranded DNA fragments of SV40 and pBR322 DNA were determined by nuclease protection techniques. Top and bottom strands of double-stranded DNA were identified by specific labeling with 32P. Protection against specific exonucleases identified binding of ADPR-t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00439a050
更新日期：1989-06-27 00:00:00
abstract：:The evolutionarily conserved DRY motif at the end of the third helix of rhodopsin-like, class-A G protein-coupled receptors (GPCRs) is a major regulator of receptor stability, signaling activity, and β-arrestin-mediated internalization. Substitution of the DRY arginine with histidine in the human vasopressin receptor ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi501061h
更新日期：2015-01-27 00:00:00
abstract：:Studies were undertaken to assess the postulated involvement of subunit III in the proton-linked functions of cytochrome c oxidase. The effect of pH on the steady-state kinetic [corrected] parameters of subunit III containing and subunit III depleted cytochrome oxidase was determined by using beef heart and rat liver ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00417a016
更新日期：1988-08-23 00:00:00
abstract：:The N-acyl- l-homoserine lactone hydrolases (AHL lactonases) have attracted considerable attention because of their ability to quench AHL-mediated quorum-sensing pathways in Gram-negative bacteria and because of their relation to other enzymes in the metallo-beta-lactamase superfamily. To elucidate the detailed cataly...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi8003704
更新日期：2008-07-22 00:00:00
abstract：:Ran, a small nuclear GTP-binding protein, is one of the most abundant Ras-related proteins in eucaryotic cells. Ran is essential for nucleo-cytoplasmatic transport and is primarily localized in the nucleus and at the nuclear pore complex. Here, we characterize the kinetics and equilibrium of the interaction between Ra...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970524k
更新日期：1997-10-07 00:00:00
abstract：:We have previously shown that the anticodon of methionine tRNAs contains most, if not all, of the nucleotides required for specific recognition of tRNA substrates by Escherichia coli methionyl-tRNA synthetase [Schulman, L. H., & Pelka, H. (1988) Science 242, 765-768]. Previous cross-linking experiments have also ident...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00461a003
更新日期：1990-03-06 00:00:00
abstract：:Thiazolinyl imine reductases catalyze the NADPH-dependent reduction of a thiazoline to a thiazolidine, a required step in the formation of the siderophores yersiniabactin (Yersinia spp.) and pyochelin (Pseudomonas aeruginosa). These stand-alone nonribosomal peptide tailoring domains are structural homologues of sugar ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00735
更新日期：2016-09-27 00:00:00
abstract：:Corticosteroid binding globulin (CBG), a serum glycoprotein which binds glucocorticoids and progestins with high affinity, is widely distributed throughout the animal world. Although its charge and size characteristics have largely been conserved across species, we found the behavior of CBG in squirrel monkey (Saimiri...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00407a046
更新日期：1988-04-05 00:00:00
abstract：:The 270-MHz 1H nuclear magnetic resonance spectra of the apo, copper(I)-zinc(II), and copper(II)-zinc(II) forms of bovine erythrocyte superoxide dismutase (EC 1.15.1.1) are reported, and assignments of resonances to ten amino acid residues are proposed. The data require that at least four and probably six histidine re...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00633a003
更新日期：1977-07-12 00:00:00
abstract：:Cells that depend on oxygen for survival constantly produce reactive oxygen species that attack DNA to produce a variety of lesions, including single-strand breaks with 3'-blocking groups such as 3'-phosphate and 3'-phosphoglycolate. These 3'-blocking ends prevent the activity of DNA polymerase and are generally remov...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi012213m
更新日期：2002-06-18 00:00:00
abstract：:The solution conformation is presented for representatives of each of the major classes of asparaginyl oligosaccharides. In this report the conformation of alpha(1-3)-, alpha(1-2)-, beta(1-2)-, and beta(1-4)-linked units is described. The conformational properties of these glycopeptides were determined by high-resolut...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00284a021
更新日期：1983-07-19 00:00:00
abstract：:The free solution electrophoretic mobilities and thermal stabilities of hairpins formed by two complementary 26-nucleotide oligomers have been measured by capillary electrophoresis. The oligomers are predicted to form molecular beacon-like hairpins with 5 bp stems and 16 nucleotides in the loop. One hairpin, called ha...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi201263n
更新日期：2011-10-25 00:00:00
abstract：:Segregation of mRNAs in the cytoplasm of polar cells has been demonstrated for proteins involved in Xenopus and Drosophila oogenesis, and for some proteins in somatic cells. It is assumed that vectorial transport of the messages is generally responsible for this localization. The mRNA encoding the basic protein of cen...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9800247
更新日期：1998-05-12 00:00:00
abstract：:Cysteine desulfurases (CDs) are pyridoxal-5'-phosphate (PLP)-dependent enzymes that cleave sulfur from cysteine via an enzyme cysteinyl persulfide intermediate. In vitro studies of these enzymes have generally employed dithiothreitol as a cosubstrate to reductively cleave the persulfide intermediate, and it has been s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049143e
更新日期：2004-09-28 00:00:00
abstract：:We examined the interactions of nucleotides with the CMP-sialic acid transporter in order to better understand which features play a role in binding and to investigate the relationship between binding and subsequent transport. With respect to the sugar, the transporter requires a complete ribose ring for tight binding...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011262w
更新日期：2001-11-27 00:00:00
abstract：:In the preceding accompanying paper [Shu, X., et al. (2007) Biochemistry 46, 12005-12013], the 1.5 A resolution crystal structure of green fluorescent protein (GFP) variant S65T/H148D is presented, and the possible consequences of an unusual short hydrogen bond ( journal_title：Biochemistry pub_type： 杂志文章 doi：10.1021/bi700904a 更新日期：2007-10-30 00:00:00
abstract：:The interactions of 4-thiouridine and 5-[(methylamino)methyl]-2-thiouridine in tRNA and of phosphorothioate esters in nucleic acids with an organomercurial have been investigated. For this purpose, an affinity electrophoretic system has been developed in which the mercury derivative has been covalently immobilized in ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00410a048
更新日期：1988-05-17 00:00:00
