当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Probing inhibitor-induced conformational changes along the interface between tissue factor and factor VIIa.

Probing inhibitor-induced conformational changes along the interface between tissue factor and factor VIIa.

Abstract：

:Upon injury of a blood vessel, activated factor VII (FVIIa) forms a high-affinity complex with its allosteric regulator, tissue factor (TF), and initiates blood clotting. Active site-inhibited factor VIIa (FVIIai) binds to TF with even higher affinity. We compared the interactions of FVIIai and FVIIa with soluble TF (sTF). Six residues in sTF were individually selected for mutagenesis and site-directed labeling. The residues are distributed along the extensive binding interface, and were chosen because they are known to interact with the different domains of FVIIa. Fluorescent and spin probes were attached to engineered Cys residues to monitor local changes in hydrophobicity, accessibility, and rigidity in the sTF--FVIIa complex upon occupation of the active site of FVIIa. The results show that inhibition of FVIIa caused the structures around the positions in sTF that interact with the protease domain of FVIIa to become more rigid and less accessible to solvent. Thus, the presence of an active site inhibitor renders the interface in this region less flexible and more compact, whereas the interface between sTF and the light chain of FVIIa is unaffected by active site occupancy.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Osterlund M,Owenius R,Carlsson K,Carlsson U,Persson E,Lindgren M,Freskgård PO,Svensson M

doi

10.1021/bi010283n

subject

Has Abstract

pub_date

2001-08-07 00:00:00

pages

9324-8

issue

31

eissn

0006-2960

issn

1520-4995

pii

bi010283n

journal_volume

40

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • L-685,458, an aspartyl protease transition state mimic, is a potent inhibitor of amyloid beta-protein precursor gamma-secretase activity.

    abstract：:Progressive cerebral amyloid beta-protein (A beta) deposition is believed to play a central role in the pathogenesis of Alzheimer's disease (AD). Elevated levels of A beta(42) peptide formation have been linked to early-onset familial AD-causing gene mutations in the amyloid beta-protein precursor (A beta PP) and the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0005456

    authors： Shearman MS,Beher D,Clarke EE,Lewis HD,Harrison T,Hunt P,Nadin A,Smith AL,Stevenson G,Castro JL

    更新日期：2000-08-01 00:00:00

  • 15N- and 13C-labeled media from Anabaena sp. for universal isotopic labeling of bacteriocins: NMR resonance assignments of leucocin A from Leuconostoc gelidum and nisin A from Lactococcus lactis.

    abstract：:A procedure for universal 13C and/or 15N labeling of microbial peptides which are produced by fermentation in complex media and its application to two food-preserving bacteriocins from lactic acid bacteria are described. Isotopic enrichment of nisin A (from Lactococcus lactis) and of leucocin A (from Leuconostoc gelid...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00052a039

    authors： Sailer M,Helms GL,Henkel T,Niemczura WP,Stiles ME,Vederas JC

    更新日期：1993-01-12 00:00:00

  • Structures of apo- and holo-tyrosine phenol-lyase reveal a catalytically critical closed conformation and suggest a mechanism for activation by K+ ions.

    abstract：:Tyrosine phenol-lyase, a tetrameric pyridoxal 5'-phosphate dependent enzyme, catalyzes the reversible hydrolytic cleavage of L-tyrosine to phenol and ammonium pyruvate. Here we describe the crystal structure of the Citrobacter freundii holoenzyme at 1.9 A resolution. The structure reveals a network of protein interact...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0601858

    authors： Milić D,Matković-Calogović D,Demidkina TV,Kulikova VV,Sinitzina NI,Antson AA

    更新日期：2006-06-20 00:00:00

  • Expression and In Vivo Characterization of the Antimicrobial Peptide Oncocin and Variants Binding to Ribosomes.

    abstract：:Peptides have important biomedical applications, but poor correlation between in vitro and in vivo activities can limit their development for clinical use. The ability to generate peptides and monitor their expression with new mass spectrometric methods and biological activities in vivo would be an advantage for the d...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.0c00600

    authors： Muthunayake NS,Islam R,Inutan ED,Colangelo W,Trimpin S,Cunningham PR,Chow CS

    更新日期：2020-09-15 00:00:00

  • Shutting Down Shigella Secretion: Characterizing Small Molecule Type Three Secretion System ATPase Inhibitors.

    abstract：:Many important human pathogens rely on one or more type three secretion systems (T3SSs) to inject bacterial effector proteins directly into the host cell cytoplasm. Secretion of protein through the needlelike type three secretion apparatus (T3SA) is essential for pathogen virulence and relies on a highly conserved ATP...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b01077

    authors： Case HB,Mattock DS,Dickenson NE

    更新日期：2018-12-18 00:00:00

  • Mechanism and role of cooperative binding of bacteriophage fd gene 5 protein to single-stranded deoxyribonucleic acid.

    abstract：:The highly cooperative binding of fd gene 5 to single-stranded DNA was studied kinetically by rapid photo-cross-linking and stopped-flow UV absorption measurements. The observed change in absorbance was shown to be due to the binding by direct evidence of rapid photo-cross-linking of the bound proteins to fd DNA. The ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00294a028

    authors： Shimamoto N,Utiyama H

    更新日期：1983-12-06 00:00:00

  • Small Molecule Rescue and Glycosidic Conformational Analysis of the Twister Ribozyme.

    abstract：:The number of self-cleaving ribozymes has increased sharply in recent years, giving rise to elaborations of the four known ribozyme catalytic strategies, α, β, γ, and δ. One such extension is utilized by the twister ribozyme, which is hypothesized to conduct δ, or general acid catalysis, via N3 of the syn adenine +1 n...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.9b00742

    authors： Messina KJ,Kierzek R,Tracey MA,Bevilacqua PC

    更新日期：2019-12-03 00:00:00

  • Acetaldehyde--enkephalins: structure proof and some conformational deductions from one- and two-dimensional proton nuclear magnetic resonance spectra.

    abstract：:The structure of the adduct formed by reaction of acetaldehyde and Met5-enkephalin has been determined by analysis of 400-MHz proton spectra: two-dimensional J spectroscopy was used to resolve and measure virtually all the overlapping resonances, and decoupling difference spectroscopy was used to assign the resonances...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00516a033

    authors： Gidley MJ,Hall LD,Sanders JK,Summers MC

    更新日期：1981-06-23 00:00:00

  • Prion protein selectively binds copper(II) ions.

    abstract：:The infectious isoform of the prion protein (PrPSc) is derived from cellular PrP (PrPC) in a conversion reaction involving a dramatic reorganization of secondary and tertiary structure. While our understanding of the pathogenic role of PrPSc has grown, the normal physiologic function of PrPC still remains unclear. Usi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi972827k

    authors： Stöckel J,Safar J,Wallace AC,Cohen FE,Prusiner SB

    更新日期：1998-05-19 00:00:00

  • Pressure effects and thermal stability of myosin rods and rod minifilaments: fluorescence and circular dichroism studies.

    abstract：:In the present study hydrostatic pressure was applied upon both skeletal myosin rod molecules and rod minifilaments to learn more of the intra- and intermolecular interaction behavior of myosin. Applied pressure disassembled the rod minifilaments into individual rod molecules and dissociated each myosin rod molecule i...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00184a028

    authors： King L,Liu CC,Lee RF

    更新日期：1994-05-10 00:00:00

  • Active site modifications in a double mutant of liver alcohol dehydrogenase: structural studies of two enzyme-ligand complexes.

    abstract：:The oxidation of alcohol to aldehyde by horse liver alcohol dehydrogenase (LADH) requires the transfer of a hydride ion from the alcohol substrate to the cofactor nicotinamide adenine dinucleotide (NAD). A quantum mechanical tunneling contribution to this hydride transfer step has been demonstrated in a number of LADH...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi973184b

    authors： Colby TD,Bahnson BJ,Chin JK,Klinman JP,Goldstein BM

    更新日期：1998-06-30 00:00:00

  • Functional characterization of an NADPH dependent 2-alkyl-3-ketoalkanoic acid reductase involved in olefin biosynthesis in Stenotrophomonas maltophilia.

    abstract：:OleD is shown to play a key reductive role in the generation of alkenes (olefins) from acyl thioesters in Stenotrophomonas maltophilia. The gene coding for OleD clusters with three other genes, oleABC, and all appear to be transcribed in the same direction as an operon in various olefin producing bacteria. In this stu...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi201096w

    authors： Bonnett SA,Papireddy K,Higgins S,del Cardayre S,Reynolds KA

    更新日期：2011-11-08 00:00:00

  • High-resolution crystal structures of the lectin-like xylan binding domain from Streptomyces lividans xylanase 10A with bound substrates reveal a novel mode of xylan binding.

    abstract：:Carbohydrate-binding module (CBM) family 13 includes the "R-type" or "ricin superfamily" beta-trefoil lectins. The C-terminal CBM, CBM13, of xylanase 10A from Streptomyces lividans is a family 13 CBM that is not only structurally similar to the "R-type" lectins but also somewhat functionally similar. The primary funct...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi015865j

    authors： Notenboom V,Boraston AB,Williams SJ,Kilburn DG,Rose DR

    更新日期：2002-04-02 00:00:00

  • The structure of chromatin: interaction of ethidium bromide with native and denatured chromatin.

    abstract：:The binding of ethidium bromide, as monitored by fluorescence enhancement, to chromatin prepared by nuclease digestion has been compared with the binding of the dye to sheared chromatin. The nuclease preparation (native chromatin) is characterized by a high affinity region of the Scatchard plot (r = 0-0.025, K1 = 1 X ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00622a002

    authors： Paoletti J,Magee BB,Magee PT

    更新日期：1977-02-08 00:00:00

  • Interaction of ribosomal protein S1 and initiation factor IF3 with the 3' major domain and the decoding site of the 30S subunit of Escherichia coli.

    abstract：:We have studied the effect of the binding of ribosomal protein S1 and initiation factor IF3 on the accessibility of nucleotide residues 584-1506 in the small subunit of the Escherichia coli ribosome. Protein S1 strongly decreases RNase V1 attack at G1164, in hairpin 40 of the 3' major domain, and weakly decreases DMS ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00112a007

    authors： Laughrea M,Tam J

    更新日期：1991-12-03 00:00:00

  • DNA sequence-specific recognition by the Saccharomyces cerevisiae "TATA" binding protein: promoter-dependent differences in the thermodynamics and kinetics of binding.

    abstract：:The equilibrium binding and association kinetics of the Saccharomyces cerevisiae TATA Binding Protein (TBP) to the E4 and Major Late promoters of adenovirus (TATATATA and TATAAAAG, respectively), have been directly compared by quantitative DNase I titration and quench-flow "footprinting". The equilibrium binding of TB...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi981072u

    authors： Petri V,Hsieh M,Jamison E,Brenowitz M

    更新日期：1998-11-10 00:00:00

  • Polyisoprenol specificity in the Campylobacter jejuni N-linked glycosylation pathway.

    abstract：:Campylobacter jejuni contains a general N-linked glycosylation pathway in which a heptasaccharide is sequentially assembled onto a polyisoprenyl diphosphate carrier and subsequently transferred to the asparagine side chain of an acceptor protein. The enzymes in the pathway function at a membrane interface and have in ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi701956x

    authors： Chen MM,Weerapana E,Ciepichal E,Stupak J,Reid CW,Swiezewska E,Imperiali B

    更新日期：2007-12-18 00:00:00

  • Calcium regulation of calmodulin binding to and dissociation from the myo1c regulatory domain.

    abstract：:Myo1c is an unconventional myosin involved in cell signaling and membrane dynamics. Calcium binding to the regulatory-domain-associated calmodulin affects myo1c motor properties, but the kinetic details of this regulation are not fully understood. We performed actin gliding assays, ATPase measurements, fluorescence sp...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi700894h

    authors： Manceva S,Lin T,Pham H,Lewis JH,Goldman YE,Ostap EM

    更新日期：2007-10-23 00:00:00

  • The Protein-tyrosine-phosphatase SHP2 is phosphorylated on serine residues 576 and 591 by protein kinase C isoforms alpha, beta 1, beta 2, and eta.

    abstract：:To study whether protein kinase C (PKC) isoforms can interact with protein-tyrosine-phosphatases (PTPs) which are connected to the insulin signaling pathway, we co-overexpressed PKC isoforms together with insulin receptor, docking proteins, and the PTPs SHP1 and SHP2 in human embryonic kidney (HEK) 293 cells. After ph...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi011327v

    authors： Strack V,Krützfeldt J,Kellerer M,Ullrich A,Lammers R,Häring HU

    更新日期：2002-01-15 00:00:00

  • Structural and Biochemical Investigation of PglF from Campylobacter jejuni Reveals a New Mechanism for a Member of the Short Chain Dehydrogenase/Reductase Superfamily.

    abstract：:Within recent years it has become apparent that protein glycosylation is not limited to eukaryotes. Indeed, in Campylobacter jejuni, a Gram-negative bacterium, more than 60 of its proteins are known to be glycosylated. One of the sugars found in such glycosylated proteins is 2,4-diacetamido-2,4,6-trideoxy-α-d-glucopyr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.7b00910

    authors： Riegert AS,Thoden JB,Schoenhofen IC,Watson DC,Young NM,Tipton PA,Holden HM

    更新日期：2017-11-14 00:00:00

  • Crystallographic Structures of IlvN·Val/Ile Complexes: Conformational Selectivity for Feedback Inhibition of Aceto Hydroxy Acid Synthases.

    abstract：:Conformational factors that predicate selectivity for valine or isoleucine binding to IlvN leading to the regulation of aceto hydroxy acid synthase I (AHAS I) of Escherichia coli have been determined for the first time from high-resolution (1.9-2.43 Å) crystal structures of IlvN·Val and IlvN·Ile complexes. The valine ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.9b00050

    authors： Bansal A,Karanth NM,Demeler B,Schindelin H,Sarma SP

    更新日期：2019-04-16 00:00:00

  • Benzothiazepine binding domain of purified L-type calcium channels: direct labeling using a novel fluorescent diltiazem analogue.

    abstract：:We have synthesized a series of N-propylamino-substituted benzazepinones (NPSBs) as specific probes for the benzothiazepinone (BTZ) binding domain of muscle L-type calcium channels (LTCCs). NPSBs were identified which possess high affinity for the channel after purification. We synthesized a fluorescent NPSB, DMBODIPY...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00010a039

    authors： Brauns T,Cai ZW,Kimball SD,Kang KC,Haugland RP,Berger W,Berjukov S,Hering S,Glossmann H,Striessnig J

    更新日期：1995-03-14 00:00:00

  • Identification of active site residues essential to 4-chlorobenzoyl-coenzyme A dehalogenase catalysis by chemical modification and site directed mutagenesis.

    abstract：:4-Chlorobenzoyl-coenzyme A (4-CBA-CoA) dehalogenase catalyzes the hydrolysis of 4-CBA-CoA to 4-hydroxybenzoyl-coenzyme A (4-HBA-CoA) via a nucleophilic aromatic substitution pathway involving the participation of an active site carboxylate side chain in covalent catalysis. In this paper we report on the identification...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9609533

    authors： Yang G,Liu RQ,Taylor KL,Xiang H,Price J,Dunaway-Mariano D

    更新日期：1996-08-20 00:00:00

  • Characterization and purification of the monoamine transporter of bovine chromaffin granules.

    abstract：:The monoamine transporter of the chromaffin granule membranes can be specifically labeled by the photoaffinity reagent 7-azido-8-[125I]iodoketanserin. The characteristics of the labeled protein have been investigated. Two-dimensional gel electrophoresis of the labeled membranes indicated a MW of about 70,000 and an is...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00122a012

    authors： Isambert MF,Gasnier B,Botton D,Henry JP

    更新日期：1992-02-25 00:00:00

  • Beta-elimination of indole from L-tryptophan catalyzed by bacterial tryptophan synthase: a comparison between reactions catalyzed by tryptophanase and tryptophan synthase.

    abstract：:Although tryptophan synthase catalyzes a number of pyridoxal phosphate dependent beta-elimination and beta-replacement reactions that are also catalyzed by tryptophanase, a principal and puzzling difference between the two enzymes lies in the apparent inability of tryptophan synthase to catalyze beta-elimination of in...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00363a010

    authors： Ahmed SA,Martin B,Miles EW

    更新日期：1986-07-29 00:00:00

  • Phosphorylation of the active, A1 component of cholera toxin by protein kinase.

    abstract：:Cholera toxin, an activator of adenylate cyclase in a wide variety of cells, is a substrate for the phosphotransferase reaction catalyzed by purified cyclic adenosine 5'-monophosphate dependent bovine cardiac muscle protein kinase and the protein associated with human erythrocyte membranes. Phosphorylation occurs when...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00658a031

    authors： Rosen OM

    更新日期：1976-06-29 00:00:00

  • Temperature dependence of forward and reverse electron transfer from A1-, the reduced secondary electron acceptor in photosystem I.

    abstract：:Electron-transfer reactions following the formation of P700(+)A1- have been studied in isolated Photosystem I complexes from Synechococcus elongatus between 300 and 5 K by flash absorption spectroscopy. (1) In the range from 300 to 200 K, A1- is reoxidized by electron transfer to the iron-sulfur cluster FX. The rate s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi973182r

    authors： Schlodder E,Falkenberg K,Gergeleit M,Brettel K

    更新日期：1998-06-30 00:00:00

  • Reversible unfolding of ribosomal protein E-L30: an NMR study.

    abstract：:Ribosomal protein E-L30 unfolds reversibly at pH values between 7.0 and 4.5. Unfolding of the protein involves a fast and a slow equilibrium, which depend on the degree of protonation of His19 and His33. Both the fast equilibrium between protonated and deprotonated histidines and the slow equilibrium between folded an...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00391a050

    authors： van de Ven FJ,Hilbers CW

    更新日期：1987-08-25 00:00:00

  • Influence of phosphoenolpyruvate and magnesium ions on the quaternary structure of enzyme I of the phosphotransferase system from gram-positive bacteria.

    abstract：:Solution X-ray scattering patterns of enzyme I of the phosphotransferase system from Staphylococcus carnosus indicate an increase in radius of gyration and molecular mass in the presence of Mg2+ or both Mg2+ and phosphoenolpyruvate, indicating a partial dimerization of enzyme I. Mg2+ ions are essential for both the di...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00048a014

    authors： Hübner G,König S,Koch MH,Hengstenberg W

    更新日期：1995-12-05 00:00:00

  • Thermodynamics of the dimer-decamer transition of reduced human and plant 2-cys peroxiredoxin.

    abstract：:Isothermal titration calorimetry (ITC) is a powerful technique for investigating self-association processes of protein complexes and was expected to reveal quantitative data on peroxiredoxin oligomerization by directly measuring the thermodynamic parameters of dimer-dimer interaction. Recombinant classical 2-cysteine ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi8002956

    authors： Barranco-Medina S,Kakorin S,Lázaro JJ,Dietz KJ

    更新日期：2008-07-08 00:00:00