Abstract：
:Peptides have important biomedical applications, but poor correlation between in vitro and in vivo activities can limit their development for clinical use. The ability to generate peptides and monitor their expression with new mass spectrometric methods and biological activities in vivo would be an advantage for the discovery and improvement of peptide-based drugs. In this study, a plasmid-based system was used to express the ribosome-targeting peptide oncocin (19 amino acids, VDKPPYLPRPRPPRRIYNR) and to determine its direct antibacterial effects on Escherichia coli. Previous biochemical and structure studies showed that oncocin targets the bacterial ribosome. The oncocin peptide generated in vivo strongly inhibits bacterial growth. In vivo dimethyl sulfate footprinting of oncocin on the rRNA gives results that are consistent with those of in vitro studies but reveals additional binding interactions with E. coli ribosomes. Furthermore, expression of truncated or mutated peptides reveals which amino acids are important for antimicrobial activity. Overall, the in vivo peptide expression system can be used to investigate biological activities and interactions of peptides with their targets within the cellular environment and to separate contributions of the sequence to cellular transport. This strategy has future applications for improving the effectiveness of existing peptides and developing new peptide-based drugs.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Muthunayake NS,Islam R,Inutan ED,Colangelo W,Trimpin S,Cunningham PR,Chow CSdoi
10.1021/acs.biochem.0c00600subject
Has Abstractpub_date
2020-09-15 00:00:00pages
3380-3391issue
36eissn
0006-2960issn
1520-4995journal_volume
59pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Pili are surface-linked virulence factors that play key roles in infection establishment in a variety of pathogenic species. In Gram-positive pathogens, pilus formation requires the action of sortases, dedicated transpeptidases that covalently associate pilus building blocks. In Streptococcus pneumoniae, a major human...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901261y
更新日期：2009-11-10 00:00:00
abstract：:To date, rational redesign of glycosidase active-site clefts has been mainly limited to the removal of essential functionalities rather than their introduction. The glycoside hydrolase family 26 endo-beta-1,4-mannanase from the soil bacterium Cellulomonas fimi depolymerizes various abundant plant mannans. On the basis...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi100097f
更新日期：2010-06-15 00:00:00
abstract：:The envelope glycoprotein (G protein) of vesicular stomatitis virus probably exists in the viral envelope as a trimer of identical subunits. Depending on the conditions of solubilization, G protein may dissociate into monomers. G protein solubilized with the detergent octyl glucoside was shown to exist as oligomeric f...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00462a002
更新日期：1990-03-13 00:00:00
abstract：:Fluorescence lifetimes and fluorescence anisotropy decays of the voltage-sensitive styryl-pyridinium dye RH421 have been measured in the presence of dimyristoylphosphatidylcholine vesicles, the water soluble enzyme ribulose 1,5-bisphosphate carboxylase/oxygenase (rubisco), and Na+,K(+)-ATPase-containing membrane fragm...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00037a015
更新日期：1995-09-19 00:00:00
abstract：:Rat alpha- and beta-parvalbumins have distinct monovalent cation-binding properties [Henzl et al. (2000) Biochemistry 39, 5859-5867]. Beta binds two Na(+) or one K(+), and alpha binds one Na(+) and no K(+). Ca(2+) abolishes these binding events, suggesting that the monovalent ions occupy the EF-hand motifs. This study...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi035890k
更新日期：2004-03-16 00:00:00
abstract：:Oligonucleotide-directed triple helix formation normally requires a long tract of oligopyrimidine.oligopurine sequence. This limitation can be partially overcome by alternate-strand triple helix (or switch triple helix) formation which enables recognition of alternating oligopurine/oligopyrimidine sequences. The prese...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980618+
更新日期：1998-09-22 00:00:00
abstract：:High-precision studies of oxygen binding in hemoglobin (HbA0) solutions at near-physiological concentrations (2-12 mM heme; pHs 7.0-9.1; various buffers) have led to an unanticipated result: an unmeasurably low contribution from the triply ligated species. We have obtained this result from new differential oxygen-bind...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00387a038
更新日期：1987-06-30 00:00:00
abstract：:The Escherichia coli D-galactose and D-glucose receptor possesses a Ca(II)-binding site closely related in structure and metal-binding characteristics to the eukaryotic EF-hand sites. Only the structure of the Ca(II)-occupied site is known. To investigate the structural change triggered by Ca(II) and Sr(II) binding, w...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00231a022
更新日期：1991-04-30 00:00:00
abstract：:To establish the state of protonation of quinonoid species formed nonenzymically from pyridoxal phosphate (PLP) and diethyl aminomalonate, we have studied absorption spectra of the rapidly established steady-state mixture of species. We have evaluated the formation constant and the spectrum of the mixture of Schiff ba...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00413a050
更新日期：1988-06-28 00:00:00
abstract：:The effect of tropomyosin alone on the binding of myosin subfragment 1 (S-1) to actin was compared to that of troponin-tropomyosin, with and without Ca2+. Over a range of ionic strength and with different ligands (adenyl-5'-yl imidodiphosphate, pyrophosphate, and adenosine 5'-diphosphate), tropomyosin confers slight c...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00280a027
更新日期：1983-05-24 00:00:00
abstract：:In an attempt to understand the mechanism by which transcription factors compete with histone octamers for cognate binding sites in chromatin, the effect of the histone binding protein nucleoplasmin on the binding of TFIIIA to nucleosomal 5S rRNA genes was tested. In this study, it was shown that, despite the previous...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972723+
更新日期：1998-02-03 00:00:00
abstract：:The free solution electrophoretic mobilities and thermal stabilities of hairpins formed by two complementary 26-nucleotide oligomers have been measured by capillary electrophoresis. The oligomers are predicted to form molecular beacon-like hairpins with 5 bp stems and 16 nucleotides in the loop. One hairpin, called ha...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi201263n
更新日期：2011-10-25 00:00:00
abstract：:Biochemical characterization of the inhibition mechanism of Deltalac-acetogenins synthesized in our laboratory indicated that they are a new type of inhibitor of bovine heart mitochondrial NADH-ubiquinone oxidoreductase (complex I) [Murai, M., et al. (2006) Biochemistry 45, 9778-9787]. To identify the binding site of ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi100454b
更新日期：2010-06-15 00:00:00
abstract：:Human DNA primase synthesizes short RNA primers that DNA polymerase alpha then elongates during the initiation of all new DNA strands. Even though primase misincorporates NTPs at a relatively high frequency, this likely does not impact the final DNA product since the RNA primer is replaced with DNA. We used an extensi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9019516
更新日期：2010-02-02 00:00:00
abstract：:Treatment of the beta 2 adrenergic receptor with the reducing agent dithiothreitol (DTT) is known to abolish ligand binding to the receptor. Interestingly, the loss of binding can be prevented by preoccupation of the receptor with ligand. It is unclear, however, whether the ligand blocks access of DTT to the receptor,...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961619+
更新日期：1996-11-19 00:00:00
abstract：:Cytochrome P450 reductase (CPR) is a tethered membrane protein which transfers electrons from NADPH to microsomal P450s. We show that the lipid bilayer has a role in defining the redox potential of the CPR flavin domains. In order to quantitate the electrochemical behavior of this central redox protein, full-length CP...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9011435
更新日期：2009-12-29 00:00:00
abstract：:Ferrochelatase is the terminal enzyme in the heme biosynthetic pathway. It catalyzes the insertion of ferrous iron into protoporphyrin IX to produce protoheme IX. The crystal structures of ferrochelatase from Saccharomyces cerevisiae in free form, in complex with Co(II), a substrate metal ion, and in complex with two ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0260785
更新日期：2002-11-19 00:00:00
abstract：:Several experimental approaches have been employed to independently determine rates of dissociation for Escherichia coli RNA polymerase from the T7 bacteriophage A1, A2, A3, and D promoters. Heparin challenge measurements employing abortive initiation turnover rates as an index of promoter occupancy were carried out o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00552a023
更新日期：1980-05-27 00:00:00
abstract：:We have performed a kinetic and thermodynamic analysis of 3D(pol) derivatives containing substitutions in the ribose-binding pocket with ATP analogues containing correct and incorrect sugar configurations. We find that Asp-238, a residue in structural motif A that is conserved in all RNA-dependent RNA polymerases, is ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi035429s
更新日期：2004-05-11 00:00:00
abstract：:The pathological prion protein PrP(Sc) is the only known component of the infectious prion. In cells infected with prions, PrP(Sc) is formed posttranslationally by the refolding of the benign cell surface glycoprotein PrP(C) into an aberrant conformation. The two PrP isoforms possess very different properties, as PrP(...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi025958g
更新日期：2002-10-22 00:00:00
abstract：:We have separately expressed the Dictyosteliumdiscoideum myosin II nonhydrolyzer point mutations E459V and E476K [Ruppel, K. M., and Spudich, J. A. (1996) Mol. Biol. Cell 7, 1123-1136] in the soluble myosin head fragment M761-1R [Anson et al. (1996) EMBO J. 15, 6069-6074] and performed transient kinetic analyses to ch...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi973143f
更新日期：1998-07-07 00:00:00
abstract：:The central importance of chorismate enzymes in bacteria, fungi, parasites, and plants combined with their absence in mammals makes them attractive targets for antimicrobials and herbicides. Two of these enzymes, anthranilate synthase (AS) and aminodeoxychorismate synthase (ADCS), are structurally and mechanistically ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00013
更新日期：2015-04-14 00:00:00
abstract：:α-Synuclein (α-Syn) oligomerization and amyloid formation are associated with Parkinson's disease (PD) pathogenesis. Studying familial α-Syn mutants associated with early onset PD has therapeutic importance. Here we report the aggregation kinetics and other biophysical properties of a newly discovered PD associated Fi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi5010365
更新日期：2014-10-21 00:00:00
abstract：:Derivatives of imidazole-2-thiones block reactions catalyzed by thyroid peroxidase (TPX) and the closely related lactoperoxidase (LPX), and this property is used therapeutically to treat hyperthyroidism. The reactions of a series of benzimidazoline-2-thiones with chemical and enzymatic oxidants were investigated to pr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00052a009
更新日期：1993-01-12 00:00:00
abstract：:Monoamine oxidase (MAO) was shown previously [Silverman, R. B., & Hoffman, S. J. (1980) J. Am. Chem. Soc. 102, 7126-7128] to catalyze the oxidation of N-cyclopropylbenzylamine (N-CBA) at two sites on the molecule. Oxidation at the benzyl methylene gave benzaldehyde and cyclopropylamine; oxidation of the cyclopropyl gr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00317a019
更新日期：1984-10-23 00:00:00
abstract：:RP2 is a ubiquitously expressed protein encoded by a gene associated with X-linked retinitis pigmentosa (XLRP), a retinal degenerative disease that causes severe vision loss. Previous in vitro studies have shown that RP2 binds to ADP ribosylation factor-like 3 (Arl3) and activates its intrinsic GTPase activity, but th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi1005249
更新日期：2010-09-07 00:00:00
abstract：:Electron paramagnetic resonance (EPR) at 15 K was used to probe the magnetic interaction between the visible copper CuA2+ and ferric cytochrome a in the carbon monoxide compound of beef heart cytochrome oxidase. At pH 8.6, the midpoint potentials (Em's) for one-electron oxidation of CuA+ and cytochrome a2+ were found ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00330a028
更新日期：1985-04-23 00:00:00
abstract：:Alpha-synuclein (alpha-syn) is a 140-residue protein that aggregates in intraneuronal inclusions called Lewy bodies in Parkinson's disease (PD). It is composed of an N-terminal domain with a propensity to bind lipids and a C-terminal domain rich in acidic residues (the acidic tail). The objective of this study was to ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060939i
更新日期：2006-09-12 00:00:00
abstract：:At neutral pH, compound I of Arthromyces ramosus peroxidase (ARP) was stable and was reduced to ferric ARP without apparent formation of compound II upon titration with ascorbate or hydroquinone. In the titration experiments, compound II was seen as an intermediate only at alkaline pH. However, measuring a difference ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00184a038
更新日期：1994-05-10 00:00:00
abstract：:In Escherichia coli, the PurT-encoded glycinamide ribonucleotide transformylase, or PurT transformylase, catalyzes an alternative formylation of glycinamide ribonucleotide (GAR) in the de novo pathway for purine biosynthesis. On the basis of amino acid sequence analyses, it is known that the PurT transformylase belong...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi000926j
更新日期：2000-08-01 00:00:00
