Phosphorylation of the active, A1 component of cholera toxin by protein kinase.

abstract：:G-protein-coupled receptors (GPCRs) represent the largest class of drug targets, accounting for more than 40% of marketed drugs; however, discovery efforts for many GPCRs have failed to provide viable drug candidates. Historically, drug discovery efforts have focused on developing ligands that act at the orthosteric s...

journal_title：Biochemistry

authors： Wood MR,Hopkins CR,Brogan JT,Conn PJ,Lindsley CW

更新日期：2011-04-05 00:00:00

abstract：:IFABP is a small beta-barrel protein with a short helix-turn-helix motif near the N-terminus that is thought to participate in the regulation of the uptake and delivery of fatty acids. In a previous work, we detected by near UV circular dichroism a reversible conformational transition of this protein occurring between...

journal_title：Biochemistry

authors： Arighi CN,Rossi JP,Delfino JM

更新日期：2003-06-24 00:00:00

abstract：:Hydrogenases are metalloenzymes that catalyze the reversible reduction of protons at unusual metal centers. This Current Topic discusses recent advances in elucidating the steps involved in the biosynthesis of the complex metal cluster at the [FeFe]-hydrogenase (HydA) active site, known as the H-cluster. The H-cluster...

journal_title：Biochemistry

authors： Shepard EM,Mus F,Betz JN,Byer AS,Duffus BR,Peters JW,Broderick JB

更新日期：2014-07-01 00:00:00

abstract：:Infrared spectroscopy has been used to monitor residual ordered structure in the denatured state of wild-type and two mutants of iso-1-cytochrome c. The technique used involves a careful digital subtraction procedure that removes spectral contributions from buffer, water vapor, and the denaturant guanidine hydrochlori...

journal_title：Biochemistry

authors： Bowler BE,Dong A,Caughey WS

更新日期：1994-03-08 00:00:00

abstract：:DNA three-way junctions (3WJs) are branched structures that serve as important biological intermediates and as components in DNA nanostructures. We recently derived the global structure of a fully complementary 3WJ and found that it contained unpaired bases at the branchpoint, which is consistent with previous observa...

journal_title：Biochemistry

authors： Toulmin A,Baltierra-Jasso LE,Morten MJ,Sabir T,McGlynn P,Schröder GF,Smith BO,Magennis SW

更新日期：2017-09-19 00:00:00

abstract：:In this paper, we report kinetic studies for the chymotryptic activity of the 20S proteasome. Major observations include the following: (1) Reaction progress curves that are recorded at concentrations of Suc-Leu-Leu-Val-Tyr-AMC greater than about 40 microM are biphasic and characterized by initial velocities that deca...

journal_title：Biochemistry

authors： Stein RL,Melandri F,Dick L

更新日期：1996-04-02 00:00:00

abstract：:We have expressed the alpha and beta subunits of bacterial luciferase, encoded by luxA and luxB, from separate plasmids in Escherichia coli and developed an efficient purification scheme that yields many milligrams of protein of greater than 90% homogeneity. Earlier experiments showed that subunits synthesized separat...

journal_title：Biochemistry

authors： Sinclair JF,Waddle JJ,Waddill EF,Baldwin TO

更新日期：1993-05-18 00:00:00

abstract：:Lecithin retinol acyltransferase (LRAT) catalyzes the esterification of all-trans-retinol into all-trans-retinyl ester, an essential reaction in the vertebrate visual cycle. Since all-trans-retinyl esters are the substrates for the isomerization reaction that generates 11-cis-retinoids, this esterification reaction is...

journal_title：Biochemistry

authors： Bok D,Ruiz A,Yaron O,Jahng WJ,Ray A,Xue L,Rando RR

更新日期：2003-05-27 00:00:00

abstract：:The DeltaG degrees (N)(-)(D) value obtained from extrapolation to zero denaturant concentration by the linear extrapolation method (LEM) is commonly interpreted to represent the Gibbs energy difference between native (N) and denatured (D) ensembles at the limit of zero denaturant concentration. For DeltaG degrees (N)(...

journal_title：Biochemistry

authors： Bolen DW,Yang M

更新日期：2000-12-12 00:00:00

abstract：:Fluorescent nucleobase analogues are used extensively to probe the structure and dynamics of nucleic acids. The fluorescence of the adenine analogue 2-aminopurine and the cytosine analogue pyrrolocytosine is significantly quenched when the bases are located in regions of double-stranded nucleic acids. To allow more de...

journal_title：Biochemistry

authors： Hardman SJ,Thompson KC

更新日期：2006-08-01 00:00:00

abstract：:A soluble Ca2+/calmodulin-dependent protein kinase has been purified from rat brain to near homogeneity by using casein as substrate. The enzyme was purified by using hydroxylapatite adsorption chromatography, phosphocellulose ion-exchange chromatography, Sepharose 6B gel filtration, affinity chromatography using calm...

journal_title：Biochemistry

authors： Kuret J,Schulman H

更新日期：1984-11-06 00:00:00

abstract：:Vibrational frequencies associated with FeC and CO stretching and FeCO bending modes have been determined via resonance Raman (RR) and infrared (IR) spectroscopy for cytochrome c peroxidase (CCP) mutants prepared by site-directed mutagenesis. These include the bacterial "wild type", CCP(MI), and mutations involving gr...

journal_title：Biochemistry

authors： Smulevich G,Mauro JM,Fishel LA,English AM,Kraut J,Spiro TG

更新日期：1988-07-26 00:00:00

abstract：:Fructose-1,6-bisphosphate muscle aldolase is an essential glycolytic enzyme that catalyzes reversible carbon-carbon bond formation by cleaving fructose 1,6-bisphosphate to yield dihydroxyacetone phosphate (DHAP) and d-glyceraldehyde phosphate. To elucidate the mechanistic role of conserved amino acid Asp-33, Asn-33 an...

journal_title：Biochemistry

authors： St-Jean M,Blonski C,Sygusch J

更新日期：2009-06-02 00:00:00

abstract：:Applications of sensitive new technologies, in particular, two-dimensional NMR spectroscopy, have allowed detection of folded structures in short peptide fragments of proteins in aqueous solution under conditions where native proteins fold. These structures are in rapid dynamic exchange with unfolded states. These obs...

journal_title：Biochemistry

authors： Wright PE,Dyson HJ,Lerner RA

更新日期：1988-09-20 00:00:00

abstract：:Faithful translation of the genetic code is mainly based on the specificity of tRNA aminoacylation catalyzed by aminoacyl-tRNA synthetases. These enzymes are comprised of a catalytic core and several appended domains. Bacterial glutamyl-tRNA synthetases (GluRS) contain five structural domains, the two distal ones inte...

journal_title：Biochemistry

authors： Dubois DY,Blais SP,Huot JL,Lapointe J

更新日期：2009-06-30 00:00:00

abstract：:In order to elucidate the molecular mechanisms responsible for the apparent nonlinear behavior of the temperature dependence of the redox potential of Hydrogenobacter thermophilus cytochrome c552 [Takahashi, Y., Sasaki, H., Takayama, S. J., Mikami, S., Kawano, S., Mita, H., Sambongi, Y., and Yamamoto, Y. (2006) Bioche...

journal_title：Biochemistry

authors： Takayama SJ,Takahashi YT,Mikami S,Irie K,Kawano S,Yamamoto Y,Hemmi H,Kitahara R,Yokoyama S,Akasaka K

更新日期：2007-08-14 00:00:00

abstract：:Several naturally occurring dietary polyphenols with chemopreventive or anticancer properties are topoisomerase II poisons. To identify additional phytochemicals that enhance topoisomerase II-mediated DNA cleavage, a library of 341 Mediterranean plant extracts was screened for activity against human topoisomerase IIα....

journal_title：Biochemistry

authors： Vann KR,Sedgeman CA,Gopas J,Golan-Goldhirsh A,Osheroff N

更新日期：2015-07-28 00:00:00

abstract：:The location of the sites of methylation of the methyl-accepting chemotaxis proteins (MCP) of Escherichia coli has been investigated by trypsin digestion and mapping of the peptides. The two principal MCPs, MCP I and MCP II, were found to have very similar methyl-labeled peptide patterns. Each produced three major spe...

journal_title：Biochemistry

authors： Chelsky D,Dahlquist FW

更新日期：1981-02-17 00:00:00

abstract：:The transporter associated with antigen processing (TAP) is a prototype of an asymmetric ATP-binding cassette (ABC) transporter, which uses ATP binding and hydrolysis to translocate peptides from the cytosol to the lumen of the endoplasmic reticulum (ER). Here, we review molecular details of peptide binding and ATP bi...

journal_title：Biochemistry

authors： Hinz A,Tampé R

更新日期：2012-06-26 00:00:00

abstract：:Although the membrane-associated surfactant protein B (SP-B) is an essential component of lung surfactant, which is itself essential for life, the molecular basis for its activity is not understood. SP-B's biophysical functions can be partially mimicked by subfragments of the protein, including the C-terminus. We have...

journal_title：Biochemistry

authors： Booth V,Waring AJ,Walther FJ,Keough KM

更新日期：2004-12-07 00:00:00

abstract：:Yeast mitochondrial NAD+-specific isocitrate dehydrogenase (IDH) has previously been shown to bind specifically to 5'-untranslated regions of yeast mitochondrial mRNAs, and transcripts containing these regions have been found to allosterically inhibit activity of the enzyme. This inhibition is relieved by AMP, an allo...

journal_title：Biochemistry

authors： Anderson SL,Schirf V,McAlister-Henn L

更新日期：2002-06-04 00:00:00

abstract：:A novel single polypeptide endopeptidase of 24 kDa (24k-endopeptidase) was purified with a yield of 300-400 microg/L from conditioned medium of a bacterial strain which was identified as a new species in the genus Chryseobacterium Sp. on the basis of its 16S rDNA sequence and DNA:DNA hybridizations. The NH(2)-terminal...

journal_title：Biochemistry

authors： Lijnen HR,Van Hoef B,Ugwu F,Collen D,Roelants I

更新日期：2000-01-18 00:00:00

abstract：:Development of potent and isoform selective nitric oxide synthase (NOS) inhibitors is challenging because of the structural similarity in the heme active sites. One amino acid difference between NOS isoforms, Asp597 in rat neuronal NOS (nNOS) versus Asn368 in bovine endothelial NOS (eNOS), has been identified as the s...

journal_title：Biochemistry

authors： Li H,Wang HY,Kang S,Silverman RB,Poulos TL

更新日期：2016-07-05 00:00:00

abstract：:Recent studies of proteins with reversed charged residues have demonstrated that electrostatic interactions on the surface can contribute significantly to protein stability. We have used the approach of reversing negatively charged residues using Arg to evaluate the effect of the electrostatics context on the transiti...

journal_title：Biochemistry

authors： Mancusso R,Cruz E,Cataldi M,Mendoza C,Fuchs J,Wang H,Yang X,Tasayco ML

更新日期：2004-04-06 00:00:00

abstract：:The interactions of a series of spin-labeled fatty acids, in which the nitroxide ring is incorporated in different ways as an integral part of the hydrocarbon chain, with the (Na+,K+)-ATPase in membranes from Squalus acanthias, have been studied by electron spin resonance spectroscopy. The fatty acids are 2,4-, 2,5-, ...

journal_title：Biochemistry

authors： Esmann M,Hideg K,Marsh D

更新日期：1988-05-31 00:00:00

abstract：:A 31 amino acid fragment of the extracellular N-terminus of the human G-protein coupled receptor for parathyroid hormone (PTH1R) has been structurally characterized by NMR and molecular dynamics simulations. The fragment PTH1R[168-198] includes residues 173-189, shown by photoaffinity cross-linking to be a contact dom...

journal_title：Biochemistry

authors： Pellegrini M,Bisello A,Rosenblatt M,Chorev M,Mierke DF

更新日期：1998-09-15 00:00:00

abstract：:19F NMR spectroscopic properties of glycogen phosphorylase reconstituted with 6-fluoropyridoxal (6-FPAL) and 6-fluoropyridoxal phosphate (6-FPLP) were investigated. Analysis of the contribution of chemical shift anisotropy to the line width of the 6-FPLP-enzyme signal shows that the coenzyme molecule is tightly bound ...

journal_title：Biochemistry

authors： Chang YC,Scott RD,Graves DJ

更新日期：1986-04-22 00:00:00

abstract：:Onconase (ONC) from Rana pipiens is the smallest member of the ribonuclease A (RNase A) superfamily. Despite a tertiary structure similar to RNase A, ONC is distinguished by an extremely high thermodynamic stability. In the present paper we have probed the significance of three structural regions, which exhibit struct...

journal_title：Biochemistry

authors： Arnold U,Schulenburg C,Schmidt D,Ulbrich-Hofmann R

更新日期：2006-03-21 00:00:00

abstract：:Lactate dehydrogenase (LDH) catalyzes the interconversion between pyruvate and lactate with nicotinamide adenine dinucleotide (NAD) as a cofactor. Using isotope-edited difference Fourier transform infrared spectroscopy on the "live" reaction mixture (LDH·NADH·pyruvate ⇌ LDH·NAD(+)·lactate) for the wild-type protein an...

journal_title：Biochemistry

authors： Peng HL,Deng H,Dyer RB,Callender R

更新日期：2014-03-25 00:00:00

abstract：:Hydrophobic interactions are responsible for stabilizing leucine zippers in peptides containing heptad repeats. The effects of substituting leucine by phenylalanine and alanine by glycine on the self-assembly of coiled-coils were examined in minimalist antimicrobial peptides designed to form amphipathic alpha-helices....

journal_title：Biochemistry

authors： Javadpour MM,Barkley MD

更新日期：1997-08-05 00:00:00