Abstract：
:Lysyl-tRNA synthetase is a member of the class II aminoacyl-tRNA synthetases and catalyses the specific aminoacylation of tRNA(Lys). The crystal structure of the constitutive lysyl-tRNA synthetase (LysS) from Escherichia coli has been determined to 2.7 A resolution in the unliganded form and in a complex with the lysine substrate. A comparison between the unliganded and lysine-bound structures reveals major conformational changes upon lysine binding. The lysine substrate is involved in a network of hydrogen bonds. Two of these interactions, one between the alpha-amino group and the carbonyl oxygen of Gly 216 and the other between the carboxylate group and the side chain of Arg 262, trigger a subtle and complicated reorganization of the active site, involving the ordering of two loops (residues 215-217 and 444-455), a change in conformation of residues 393-409, and a rotation of a 4-helix bundle domain (located between motif 2 and 3) by 10 degrees. The result of these changes is a closing up of the active site upon lysine binding.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Onesti S,Desogus G,Brevet A,Chen J,Plateau P,Blanquet S,Brick Pdoi
10.1021/bi001487rsubject
Has Abstractpub_date
2000-10-24 00:00:00pages
12853-61issue
42eissn
0006-2960issn
1520-4995pii
bi001487rjournal_volume
39pub_type
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