Structural Analysis Provides Mechanistic Insight into Nicotine Oxidoreductase from Pseudomonas putida.

abstract：:Halogen bonding is a weak chemical force that has so far mostly found applications in crystal engineering. Despite its potential for use in drug discovery, as a new molecular tool in the direction of molecular recognition events, it has rarely been assessed in biopolymers. Motivated by this fact, we have developed a p...

journal_title：Biochemistry

authors： Danelius E,Andersson H,Jarvoll P,Lood K,Gräfenstein J,Erdélyi M

更新日期：2017-06-27 00:00:00

abstract：:Modification of the bacterial cell wall heteropolymer peptidoglycan by addition of an acetyl group to the C-6 hydroxyl group of N-acetylmuramoyl residues is known to inhibit the activity of muramidases (lysozymes) of innate immune systems. The O-acetylation of peptidoglycan also precludes the action of intrinsic lytic...

journal_title：Biochemistry

authors： Weadge JT,Clarke AJ

更新日期：2006-01-24 00:00:00

abstract：:The assignment of disulfide bonds in human J chain and its covalent pairing with immunoglobulin M was determined under conditions which minimize disulfide bond interchange. We show that in J chain the three intradisulfide bridges are formed between Cys 12 and 100, Cys 71 and 91, and Cys 108 and 133. Previous reports [...

journal_title：Biochemistry

authors： Frutiger S,Hughes GJ,Paquet N,Lüthy R,Jaton JC

更新日期：1992-12-22 00:00:00

abstract：:Picosecond transient absorption spectra of rhodopsin and isorhodopsin were measured at room temperature with a double-beam laser spectrophotometer after excitation at 355 nm. Photolysis studies were performed on rhodopsin solubilized in two different detergents (digitonin and 3-[(3-cholamidopropyl)dimethylammonio]-1-p...

journal_title：Biochemistry

authors： Rudzki JE,Peters KS

更新日期：1984-08-14 00:00:00

abstract：:The 14-3-3 proteins interact with diverse cellular molecules involved in various signal transduction pathways controlling cell proliferation, transformation, and apoptosis. To aid our investigation of the biological function of 14-3-3 proteins, we have set out to identify high-affinity antagonists. By screening phage ...

journal_title：Biochemistry

authors： Wang B,Yang H,Liu YC,Jelinek T,Zhang L,Ruoslahti E,Fu H

更新日期：1999-09-21 00:00:00

abstract：:The fungal neurotoxin alpha-cyclopiazonic acid (CPA), a nanomolar inhibitor of Ca(2+)-ATPase with a unique pentacyclic indole tetramic acid scaffold, is assembled by a three enzyme pathway CpaS, CpaD, and CpaO in Aspergillus sp. We recently characterized the first pathway-specific enzyme CpaS, a hybrid two module poly...

journal_title：Biochemistry

authors： Liu X,Walsh CT

更新日期：2009-11-24 00:00:00

abstract：:Factor V(a) (FV(a)) is a cofactor for the serine protease factor X(a) that activates prothrombin to thrombin in the presence of Ca(2+) and a membrane surface. FV(a) is a heterodimer composed of one heavy chain (A1 and A2 domains) and one light chain (A3, C1, and C2 domains). We use fluorescence, circular dichroism, an...

journal_title：Biochemistry

authors： Srivastava A,Quinn-Allen MA,Kim SW,Kane WH,Lentz BR

更新日期：2001-07-27 00:00:00

abstract：:Rat organic anion transporting protein 1a1 (oatp1a1), a hepatocyte basolateral plasma membrane protein, mediates transport of various amphipathic compounds. Our previous studies indicated that serine phosphorylation of a single tryptic peptide inhibits its transport activity without changing its cell surface content. ...

journal_title：Biochemistry

authors： Xiao Y,Nieves E,Angeletti RH,Orr GA,Wolkoff AW

更新日期：2006-03-14 00:00:00

abstract：:The interactions of bovine brain MAP2 and tau, recombinant murine MAP2C, and recombinant human tau with phosphatidylinositol vesicles yield apparent Kd values of 51 +/- 6 nM, 2.4 +/- 0.6 microM, 1.4 +/- 0.1 microM, and 1.6 +/- 0.2 microM, respectively. Examinations of the binding of MAP2 and/or MAP2C to phosphatidylch...

journal_title：Biochemistry

authors： Surridge CD,Burns RG

更新日期：1994-07-05 00:00:00

abstract：:Ferrochelatase is the terminal enzyme in the heme biosynthetic pathway. It catalyzes the insertion of ferrous iron into protoporphyrin IX to produce protoheme IX. The crystal structures of ferrochelatase from Saccharomyces cerevisiae in free form, in complex with Co(II), a substrate metal ion, and in complex with two ...

journal_title：Biochemistry

authors： Karlberg T,Lecerof D,Gora M,Silvegren G,Labbe-Bois R,Hansson M,Al-Karadaghi S

更新日期：2002-11-19 00:00:00

abstract：:In the anaerobic sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough (DvH), the genome sequencing revealed the presence of three operons encoding formate dehydrogenases. fdh1 encodes an alphabetagamma trimeric enzyme containing 11 heme binding sites; fdh2 corresponds to an alphabetagamma trimeric enzyme wi...

journal_title：Biochemistry

authors： ElAntak L,Dolla A,Durand MC,Bianco P,Guerlesquin F

更新日期：2005-11-15 00:00:00

abstract：:To understand the stabilization, folding, and functional mechanisms of proteins, it is very important to understand the structural and thermodynamic properties of the molten globule state. In this study, the global structure of the acid molten globule state, which we call MG1, of horse cytochrome c at low pH and high ...

journal_title：Biochemistry

authors： Nakamura S,Seki Y,Katoh E,Kidokoro S

更新日期：2011-04-19 00:00:00

abstract：:Nitridomanganese(V) protoporphyrin IX was prepared by hypochlorite oxidation of the corresponding manganese(III) protoporphyrin IX derivative in the presence of ammonium ion and by photolysis of the corresponding azidomanganese(III) complex. Myoglobin and horseradish peroxidase containing this novel protoporphyrin der...

journal_title：Biochemistry

authors： Tsubaki M,Hori H,Hotta T,Hiwatashi A,Ichikawa Y,Yu NT

更新日期：1987-08-11 00:00:00

abstract：:Under mildly acidic conditions, annexin 12 (ANX) inserts into lipid membranes to form a transbilayer pore [Langen, R., et al. (1998) Proc. Natl. Acad. Sci. U.S.A. 95, 14060]. In this study, we have addressed the question of the oligomeric state of ANX in this transbilayer conformation by means of Forster-type resonanc...

journal_title：Biochemistry

authors： Ladokhin AS,Haigler HT

更新日期：2005-03-08 00:00:00

abstract：:The structure of a synthetic peptide corresponding to residues 1-45 of rabbit muscle adenylate kinase has been studied in aqueous solution by two-dimensional NMR, FTIR, and CD spectroscopy. This peptide, which binds MgATP and is believed to represent most of the MgATP-binding site of the enzyme [Fry, D.C., Kuby, S.A.,...

journal_title：Biochemistry

authors： Fry DC,Byler DM,Susi H,Brown EM,Kuby SA,Mildvan AS

更新日期：1988-05-17 00:00:00

abstract：:The phagocyte superoxide-generating NADPH oxidase, a multicomponent, membrane-bound electron transport chain, consists of cytochrome b558, p47-phox, p67-phox, and p21rac1 or p21rac2. The mechanisms of oxidase assembly are poorly understood. In previous studies using a cell-free NADPH oxidase system, we showed that pre...

journal_title：Biochemistry

authors： Kleinberg ME,Malech HL,Mital DA,Leto TL

更新日期：1994-03-08 00:00:00

abstract：:In addition to encoding the tertiary fold and stability, the primary sequence of a protein encodes the folding trajectory and kinetic barriers that determine the speed of folding. How these kinetic barriers are encoded is not well understood. Here, we use evolutionary sequence variation in the α-lytic protease (αLP) p...

journal_title：Biochemistry

authors： Nixon CF,Lim SA,Sailer ZR,Zheludev IN,Gee CL,Kelch BA,Harms MJ,Marqusee S

更新日期：2021-01-26 00:00:00

abstract：:Biological actions of insulin are initiated by activation of the insulin receptor tyrosine kinase. Protein tyrosine phosphatases (PTPases) PTP1B and PTPalpha are known to dephosphorylate the insulin receptor and may contribute to insulin resistance in diseases such as diabetes. We previously reported that overexpressi...

journal_title：Biochemistry

authors： Chen H,Cong LN,Li Y,Yao ZJ,Wu L,Zhang ZY,Burke TR Jr,Quon MJ

更新日期：1999-01-05 00:00:00

abstract：:NADPH-cytochrome P450 oxidoreductase (P450 reductase, EC 1.6.2.4) is an essential component of the P450 monooxygenase complex and binds FMN, FAD, and NADPH cofactors. Residues Tyr140 and Tyr178 are known to be involved in FMN binding. A third aromatic side chain, Phe181, is also located in the proximity of the FMN rin...

journal_title：Biochemistry

authors： Paine MJ,Ayivor S,Munro A,Tsan P,Lian LY,Roberts GC,Wolf CR

更新日期：2001-11-13 00:00:00

abstract：:The conformational analysis of non-native states of proteins remains one of the most difficult problems in structural biology, because such states are represented by a superimposition of several states that are rapidly interconverting. Hence, model building of the conformational ensemble remains challenging, although ...

journal_title：Biochemistry

authors： Sziegat F,Silvers R,Hähnke M,Jensen MR,Blackledge M,Wirmer-Bartoschek J,Schwalbe H

更新日期：2012-04-24 00:00:00

abstract：:We have synthesized 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho(N-oleoyl)serine (N-acyl-PS) and 1,2-dioleoyl-sn-glycero-3-phospho-Tris (phosphatidyl-Tris) and have characterized both phospholipids by their chemical and chromatographic properties, as well as by their IR, 13C NMR, and 1H NMR spectra. Comparison of these d...

journal_title：Biochemistry

authors： Schmid PC,Kumar VV,Weis BK,Schmid HH

更新日期：1991-02-19 00:00:00

abstract：:A rapid, three-step purification of DNA alpha-polymerase from calf thymus is described. The key feature is immunoaffinity chromatography using a column of immobilized monoclonal immunoglobulin G (IgG) developed against human KB cell alpha-polymerase. This step is followed by preparative sucrose gradient sedimentation....

journal_title：Biochemistry

authors： Wahl AF,Kowalski SP,Harwell LW,Lord EM,Bambara RA

更新日期：1984-04-24 00:00:00

abstract：:Although the short-chain dehydrogenase/reductase (SDR) superfamily contains a very large number of members defined in annotated databases and by biochemical and structural studies, very few SDR enzymes have been identified that have a homologous partner catalyzing the same reaction but with an opposite stereospecifici...

journal_title：Biochemistry

authors： Clark DD,Ensign SA

更新日期：2002-02-26 00:00:00

abstract：:The effects of several guanine nucleotide analogues on (Na+,K+)-ATPase activity of membranes isolated from several tissues were analyzed to determine if a G-protein might be involved in the hormonal regulation of the (Na+,K+)-ATPase. Submillimolar concentrations of GTP gamma S, but not GMPPNP, inhibit rat skeletal mus...

journal_title：Biochemistry

authors： McGill DL

更新日期：1991-07-09 00:00:00

abstract：:Murine double-minute clone 2 protein (MDM2) is an E3 ubiquitin ligase that regulates the turnover of several cellular factors including the p53 tumor suppressor protein. As part of the mechanism of p53 induction in response to DNA damage, a cluster of serine residues within the central acidic domain of MDM2 become hyp...

journal_title：Biochemistry

authors： Winter M,Milne D,Dias S,Kulikov R,Knippschild U,Blattner C,Meek D

更新日期：2004-12-28 00:00:00

abstract：:Gelatinase B is a matrix metalloproteinase (MMP-9) involved in tissue remodeling, development, cancer, and inflammation. Neutrophils produce three major forms of (pro)gelatinase B: 92 kDa monomers, homodimers, and complexes of gelatinase B covalently bound to neutrophil gelatinase B-associated lipocalin (NGAL). In con...

journal_title：Biochemistry

authors： Rudd PM,Mattu TS,Masure S,Bratt T,Van den Steen PE,Wormald MR,Küster B,Harvey DJ,Borregaard N,Van Damme J,Dwek RA,Opdenakker G

更新日期：1999-10-19 00:00:00

abstract：:As a prerequisite to mammalian fertilization, the sperm acrosomal vesicle fuses with the plasma membrane and the acrosome contents are exocytosed. Induction occurs through engagement of the sperm receptors by multiple sugar residues. Multivalent polymers displaying mannose, fucose, or GlcNAc are effective synthetic in...

journal_title：Biochemistry

authors： Huang H,Rodolis MT,Bhatia SR,Sampson NS

更新日期：2017-06-06 00:00:00

abstract：:Binding studies of the interaction of immobilized 1alpha- and 17alpha-aminoalkyl derivatives of 5alpha-dihydrotestosterone (DHT) with purified N-deglycosylated homodimeric human sex hormone-binding globulin (SHBG) were performed using a surface plasmon resonance biosensor. These 1alpha- and 17alpha-derivatives with sp...

journal_title：Biochemistry

authors： Metzger J,Schnitzbauer A,Meyer M,Söder M,Cuilleron CY,Hauptmann H,Huber E,Luppa PB

更新日期：2003-11-25 00:00:00

abstract：:The reaction of cytochrome c oxidase (COX) from Rhodobacter sphaeroides with hydrogen peroxide has been studied at alkaline (pH 8.5) and acidic (pH 6.5) conditions with the aid of a stopped-flow apparatus. Absorption changes in the entire 350-800 nm spectral range were monitored and analyzed by a global fitting proced...

journal_title：Biochemistry

authors： Pecoraro C,Gennis RB,Vygodina TV,Konstantinov AA

更新日期：2001-08-14 00:00:00

abstract：:Matrix metalloproteinases (MMPs) are a family of enzymes that are up-regulated in many diseases, including osteoarthritis (OA) and rheumatoid arthritis (RA). Here we report on a novel technique that can be used to simultaneously measure activity levels for a panel of enzymes, such as the MMPs. The technique, termed th...

journal_title：Biochemistry

authors： Rasmussen FH,Yeung N,Kiefer L,Murphy G,Lopez-Otin C,Vitek MP,Moss ML

更新日期：2004-03-23 00:00:00