Phosphatidylinositol 4,5-bisphosphate alters synaptotagmin 1 membrane docking and drives opposing bilayers closer together.


:Synaptotagmin 1 (syt1) is a synaptic vesicle-anchored membrane protein that acts as the calcium sensor for the synchronous component of neuronal exocytosis. Using site-directed spin labeling, the position and membrane interactions of a fragment of syt1 containing its two C2 domains (syt1C2AB) were assessed in bilayers containing phosphatidylcholine (PC), phosphatidylserine (PS), and phosphatidylinositol 4,5-bisphosphate (PIP(2)). Addition of 1 mol % PIP(2) to a lipid mixture of PC and PS results in a deeper membrane penetration of the C2A domain and alters the orientation of the C2B domain so that the polybasic face of C2B comes into the proximity of the bilayer interface. The C2B domain is found to contact the membrane interface in two regions, the Ca(2+)-binding loops and a region opposite the Ca(2+)-binding loops. This suggests that syt1C2AB is configured to bridge two bilayers and is consistent with a model generated previously for syt1C2AB bound to membranes of PC and PS. Point-to-plane depth restraints, obtained by progressive power saturation, and interdomain distance restraints, obtained by double electron-electron resonance, were obtained in the presence of PIP(2) and used in a simulated annealing routine to dock syt1C2AB to two membrane interfaces. The results yield an average structure different from what is found in the absence of PIP(2) and indicate that bilayer-bilayer spacing is decreased in the presence of PIP(2). The results indicate that PIP(2), which is necessary for bilayer fusion, alters C2 domain orientation, enhances syt1-membrane electrostatic interactions, and acts to drive vesicle and cytoplasmic membrane surfaces closer together.






Kuo W,Herrick DZ,Cafiso DS




Has Abstract


2011-04-05 00:00:00












  • n-Alkylboronic acid inhibitors reveal determinants of ligand specificity in the quorum-quenching and siderophore biosynthetic enzyme PvdQ.

    abstract::The enzyme PvdQ (E.C. from Pseudomonas aeruginosa is an N-terminal nucleophile hydrolase that catalyzes the removal of an N-myristyl substituent from a biosynthetic precursor of the iron-chelating siderophore pyoverdine. Inhibitors of pyoverdine biosynthesis are potential antibiotics since iron is essential ...


    pub_type: 杂志文章


    authors: Clevenger KD,Wu R,Liu D,Fast W

    更新日期:2014-10-28 00:00:00

  • Direct measurement of nucleation and growth rates in lysozyme folding.

    abstract::A kinetic folding intermediate of hen lysozyme is shown to form in a nucleation/growth type of mechanism. Under native solvent conditions, a nucleated state is formed slowly during refolding (tau = 14 +/- 1 ms at 0 M GdmCl) and is rapidly converted to the folding intermediate (tau = 300 +/- 150 micros at 0 M GdmCl). U...


    pub_type: 杂志文章


    authors: Kiefhaber T,Bachmann A,Wildegger G,Wagner C

    更新日期:1997-04-29 00:00:00

  • Monovalent cation transport: lack of structural deformation upon cation binding.

    abstract::Cations often deform the structure of regulatory proteins to affect a functional response, but for other protein functions a more passive effect is desired. For instance, it is shown here that in the conductance of Na+ by the gramicidin channel there appears to be no significant structural deformation of either the si...


    pub_type: 杂志文章


    authors: Tian F,Lee KC,Hu W,Cross TA

    更新日期:1996-09-17 00:00:00

  • DCCD inhibits the reactions of the iron-sulfur protein in Rhodobacter sphaeroides chromatophores.

    abstract::N,N'-dicyclohexylcarbodiimide (DCCD) has been reported to inhibit proton translocation by cytochrome bc(1) and b(6)f complexes without significantly altering the rate of electron transport, a process referred to as decoupling. To understand the possible role of DCCD in inhibiting the protonogenic reactions of cytochro...


    pub_type: 杂志文章


    authors: Shinkarev VP,Ugulava NB,Crofts AR,Wraight CA

    更新日期:2000-12-26 00:00:00

  • 9-cis-retinoids: biosynthesis of 9-cis-retinoic acid.

    abstract::Retinoids function through conformational alterations of ligand-dependent nuclear transcription factors, the retinoic acid receptors, and retinoid X receptors. 9-cis-Retinoic acid is a known biological ligand for retinoid X receptors, but its synthesis pathway in vivo is largely unknown. Recently, we identified a cis-...


    pub_type: 杂志文章


    authors: Paik J,Vogel S,Piantedosi R,Sykes A,Blaner WS,Swisshelm K

    更新日期:2000-07-11 00:00:00

  • Dynamic nature of the quaternary structure of the vesicular stomatitis virus envelope glycoprotein.

    abstract::The envelope glycoprotein (G protein) of vesicular stomatitis virus probably exists in the viral envelope as a trimer of identical subunits. Depending on the conditions of solubilization, G protein may dissociate into monomers. G protein solubilized with the detergent octyl glucoside was shown to exist as oligomeric f...


    pub_type: 杂志文章


    authors: Lyles DS,Varela VA,Parce JW

    更新日期:1990-03-13 00:00:00

  • Biostructural chemistry of magnesium ion: characterization of the weak binding sites on tRNA(Phe)(yeast). Implications for conformational change and activity.

    abstract::The thermodynamics and kinetics of magnesium binding to tRNA(Phe)(yeast) have been studied directly by 25Mg NMR. In 0.17 M Na+(aq), tRNA(Phe) exists in its native conformation and the number of strong binding sites (Ka greater than or equal to 10(4)) was estimated to be 3-4 by titration experiments, in agreement with ...


    pub_type: 杂志文章


    authors: Reid SS,Cowan JA

    更新日期:1990-06-26 00:00:00

  • (15)N NMR relaxation data reveal significant chemical exchange broadening in the alpha-domain of human alpha-lactalbumin.

    abstract::Human alpha-lactalbumin (alpha-LA), a 123-residue calcium-binding protein, has been studied using (15)N NMR relaxation methods in order to characterize backbone dynamics of the native state at the level of individual residues. Relaxation data were collected at three magnetic field strengths and analyzed using the mode...


    pub_type: 杂志文章


    authors: Bruylants G,Redfield C

    更新日期:2009-05-19 00:00:00

  • Insight into the mechanism of inactivation of ribonucleotide reductase by gemcitabine 5'-diphosphate in the presence or absence of reductant.

    abstract::Gemcitabine 5'-diphosphate (F(2)CDP) is a potent inhibitor of ribonucleotide reductases (RNRs), enzymes that convert nucleotides (NDPs) to deoxynucleotides and are essential for DNA replication and repair. The Escherichia coli RNR, an alpha2beta2 complex, when incubated with 1 equiv of F(2)CDP catalyzes the release of...


    pub_type: 杂志文章


    authors: Artin E,Wang J,Lohman GJ,Yokoyama K,Yu G,Griffin RG,Bar G,Stubbe J

    更新日期:2009-12-15 00:00:00

  • Differential interaction of lecithin-retinol acyltransferase with cellular retinol binding proteins.

    abstract::Esterification of retinol (vitamin A alcohol) with long-chain fatty acids by lecithin-retinol acyltransferase (LRAT) is an important step in both the absorption and storage of vitamin A. Retinol in cells is bound by either cellular retinol binding protein (CRBP), present in most tissues including liver, or cellular re...


    pub_type: 杂志文章


    authors: Herr FM,Ong DE

    更新日期:1992-07-28 00:00:00

  • Subunit structure of human erythrocyte glycophorin A.

    abstract::Glycophorin A is a sialoglycoprotein isolated from human erythrocyte membranes which seems to exist as stable dimeric complexes in the presence of sodium dodecyl sulfate. When analyzed by dodecyl sulfate acrylamide electrophoresis this molecule forms two PAS-stainable bands (PAS-U and PAS-2) which are reversibly inter...


    pub_type: 杂志文章


    authors: Furthmayr H,Marchesi VT

    更新日期:1976-03-09 00:00:00

  • Single-channel recordings from purified acetylcholine receptors reconstituted in bilayers formed at the tip of patch pipets.

    abstract::The channel of the purified acetylcholine receptor from Torpedo californica electric organ reconstituted in lipid vesicles was assayed by direct electrical recording using patch-clamp pipets. High-resistance seals were obtained by gentle suction of vesicles into the pipet or after the formation of lipid bilayers from ...


    pub_type: 杂志文章


    authors: Suarez-Isla BA,Wan K,Lindstrom J,Montal M

    更新日期:1983-05-10 00:00:00

  • X-ray crystal structure of Desulfovibrio vulgaris rubrerythrin with zinc substituted into the [Fe(SCys)4] site and alternative diiron site structures.

    abstract::The X-ray crystal structure of recombinant Desulfovibrio vulgaris rubrerythrin (Rbr) that was subjected to metal constitution first with zinc and then iron, yielding ZnS(4)Rbr, is reported. A [Zn(SCys)(4)] site with no iron and a diiron site with no appreciable zinc in ZnS(4)Rbr were confirmed by analysis of the anoma...


    pub_type: 杂志文章


    authors: Jin S,Kurtz DM Jr,Liu ZJ,Rose J,Wang BC

    更新日期:2004-03-23 00:00:00

  • Acid-induced unfolding and refolding transitions of cytochrome c: a three-state mechanism in H2O and D2O.

    abstract::Whereas the salt-dependent conformational transition of acid-denatured horse ferricytochrome c at pH 2 is approximated by a two-state mechanism from the acid-unfolded state to the molten globule state [Kataoka, M., Hagihara, Y., Mihara, K., & Goto, Y. (1993) J. Mol. Biol. 229, 591-596], the corresponding transition in...


    pub_type: 杂志文章


    authors: Goto Y,Hagihara Y,Hamada D,Hoshino M,Nishii I

    更新日期:1993-11-09 00:00:00

  • Isolation of high-affinity peptide antagonists of 14-3-3 proteins by phage display.

    abstract::The 14-3-3 proteins interact with diverse cellular molecules involved in various signal transduction pathways controlling cell proliferation, transformation, and apoptosis. To aid our investigation of the biological function of 14-3-3 proteins, we have set out to identify high-affinity antagonists. By screening phage ...


    pub_type: 杂志文章


    authors: Wang B,Yang H,Liu YC,Jelinek T,Zhang L,Ruoslahti E,Fu H

    更新日期:1999-09-21 00:00:00

  • Internal architecture of the core nucleosome: fluorescence energy transfer studies at methionine-84 of histone H4.

    abstract::Chicken histone H4, labeled separately at Met-84 with N-[[(iodoacetyl)amino]ethyl]-5-naphthylamine-1-sulfonic acid and 5-(iodoacetamido)fluorescein, was reassociated with unlabeled histones H2A, H2B, and H3 and 146 base pairs of DNA to produce fluorescently labeled nucleosomes having physical characteristics virtually...


    pub_type: 杂志文章


    authors: Chung DG,Lewis PN

    更新日期:1986-09-09 00:00:00

  • Metal binding at the Deinococcus radiodurans Dps-1 N-terminal metal site controls dodecameric assembly and DNA binding.

    abstract::The prokaryotic DNA protection during starvation (Dps) proteins typically protect macromolecules against damaging agents via physical association with DNA and by oxidizing and sequestering iron. However, Deinococcus radiodurans Dps-1, which binds DNA with high affinity, fails to protect DNA against hydroxyl radicals d...


    pub_type: 杂志文章


    authors: Nguyen KH,Grove A

    更新日期:2012-08-21 00:00:00

  • Spectroscopic and kinetic studies of lipases solubilized in reverse micelles.

    abstract::The conformation and activity of three different lipases have been studied in reverse micelles formed by sodium bis(2-ethylhexyl) sulfosuccinate (AOT) in isooctane. In the case of human pancreatic lipase, the conformation of the polypeptide chain--as judged from far-UV circular dichroism measurements--is only slightly...


    pub_type: 杂志文章


    authors: Walde P,Han D,Luisi PL

    更新日期:1993-04-20 00:00:00

  • Conversion of a cosubstrate to an inhibitor: phosphorylation mutants of nicotinic acid phosphoribosyltransferase.

    abstract::Nicotinic acid phosphoribosyltransferase (NAPRTase; EC forms nicotinic acid mononucleotide (NAMN) and PPi from 5-phosphoribosyl 1-pyrophosphate (PRPP) and nicotinic acid (NA). The Vmax NAMN synthesis activity of the Salmonella typhimurium enzyme is stimulated about 10-fold by ATP, which, when present, is hyd...


    pub_type: 杂志文章


    authors: Rajavel M,Lalo D,Gross JW,Grubmeyer C

    更新日期:1998-03-24 00:00:00

  • Site-directed mutagenesis and molecular modeling identify a crucial amino acid in specifying the heparin affinity of FGF-1.

    abstract::Heparin potentiates the mitogenic activity of FGF-1 by increasing the affinity for its receptor and by extending its biological half-life. During the course of labeling human FGF-1 with Na(125)I and chloramine T, it was observed that the protein lost its ability to bind to heparin. In contrast, bovine FGF-1 retained i...


    pub_type: 杂志文章


    authors: Patrie KM,Botelho MJ,Franklin K,Chiu IM

    更新日期:1999-07-20 00:00:00

  • Further characterization of the spin coupling observed in oxidized hydrogenase from Chromatium vinosum. A Mössbauer and multifrequency EPR study.

    abstract::Hydrogenase from Chromatium vinosum contains 1 Ni, 11-12 Fe, and ca. 9 sulfides. EPR and Mössbauer studies of the enzyme prepared in four different oxidation states show that the enzyme contains two Fe4S4 and one Fe3S4 cluster. In the oxidized (2+) state, the Mössbauer parameters of the two Fe4S4 clusters are typical ...


    pub_type: 杂志文章


    authors: Surerus KK,Chen M,van der Zwaan JW,Rusnak FM,Kolk M,Duin EC,Albracht SP,Münck E

    更新日期:1994-04-26 00:00:00

  • Relationship between the native-state hydrogen exchange and folding pathways of a four-helix bundle protein.

    abstract::The hydrogen exchange behavior of a four-helix bundle protein in low concentrations of denaturant reveals some partially unfolded forms that are significantly more stable than the fully unfolded state. Kinetic folding of the protein, however, is apparently two-state in the absence of the accumulation of early folding ...


    pub_type: 杂志文章


    authors: Chu R,Pei W,Takei J,Bai Y

    更新日期:2002-06-25 00:00:00

  • Conservation of a helix-stabilizing dipole moment in the PP-fold family of regulatory peptides.

    abstract::Investigation of the charge distribution for all known members of the PP-fold family of peptides reveals a common pattern characterized by a cluster of negative charges in the beta-turn region and a cluster of positive charges in the receptor-binding region of the peptide. Detailed analysis of the electrostatic proper...


    pub_type: 杂志文章


    authors: Bjørnholm B,Jørgensen FS,Schwartz TW

    更新日期:1993-03-30 00:00:00

  • Reactions of isocytochrome c2 in the photosynthetic electron transfer chain of Rhodobacter sphaeroides.

    abstract::Rhodobacter sphaeroides strains lacking cytochrome c2 (cyt c2), the normal electron donor to P870+ in light-oxidized reaction center (RC) complexes, are unable to grow photosynthetically. However, spd mutations that suppress the photosynthetic deficiency of cyt c2 mutants elevate levels of the cyt c2 isoform, isocyt c...


    pub_type: 杂志文章


    authors: Witthuhn VC Jr,Gao J,Hong S,Halls S,Rott MA,Wraight CA,Crofts AR,Donohue TJ

    更新日期:1997-01-28 00:00:00

  • Surface loops adjacent to the cation-binding site of the complement factor B von Willebrand factor type A module determine C3b binding specificity.

    abstract::The interaction of factor B with C3b deposited on the surface of pathogens is the first step in the activation of the alternative complement pathway. The role of the von Willebrand factor type A (VWFA) module of factor B in this interaction has been investigated by generating three chimeras, Ch1-Ch3, in which surface ...


    pub_type: 杂志文章


    authors: Tuckwell DS,Xu Y,Newham P,Humphries MJ,Volanakis JE

    更新日期:1997-06-03 00:00:00

  • Solution structure of the 45-residue MgATP-binding peptide of adenylate kinase as examined by 2-D NMR, FTIR, and CD spectroscopy.

    abstract::The structure of a synthetic peptide corresponding to residues 1-45 of rabbit muscle adenylate kinase has been studied in aqueous solution by two-dimensional NMR, FTIR, and CD spectroscopy. This peptide, which binds MgATP and is believed to represent most of the MgATP-binding site of the enzyme [Fry, D.C., Kuby, S.A.,...


    pub_type: 杂志文章


    authors: Fry DC,Byler DM,Susi H,Brown EM,Kuby SA,Mildvan AS

    更新日期:1988-05-17 00:00:00

  • Synthesis of dihydrothymidine and thymidine glycol 5'-triphosphates and their ability to serve as substrates for Escherichia coli DNA polymerase I.

    abstract::5,6-Dihydrothymidine 5'-triphosphate (DHdTTP) was synthesized by catalytic hydrogenation of thymidine 5'-triphosphate (dTTP). Thymidine glycol 5'-triphosphate (dTTP-GLY) was prepared by bromination of dTTP followed by treatment with Ag2O. The modified nucleotides were extensively purified by anion-exchange high-perfor...


    pub_type: 杂志文章


    authors: Ide H,Melamede RJ,Wallace SS

    更新日期:1987-02-10 00:00:00

  • Purification and characterization of corn glutathione S-transferase.

    abstract::Two glutathione S-transferase (GST) activities have been identified and purified from etiolated corn tissue. The first, designated GST I enzyme, is constitutively present in corn tissue, and the second, designated GST II enzyme, is present only in tissue which has been treated with chemical antidotes which protect cor...


    pub_type: 杂志文章


    authors: Mozer TJ,Tiemeier DC,Jaworski EG

    更新日期:1983-03-01 00:00:00

  • Equilibrium folding studies of cellular retinoic acid binding protein, a predominantly beta-sheet protein.

    abstract::We have examined the conformational behavior under various unfolding conditions of a predominantly beta-sheet protein, cellular retinoic acid binding protein (CRABP). Urea unfolding-refolding of CRABP is a highly cooperative process that can be approximated by a two-state model. Acid denaturation is also cooperative a...


    pub_type: 杂志文章


    authors: Liu ZP,Rizo J,Gierasch LM

    更新日期:1994-01-11 00:00:00

  • Effects of recombinant protein expression on green fluorescent protein diffusion in Escherichia coli.

    abstract::Fluorescence recovery after photobleaching was used to measure the diffusion coefficient of green fluorescent protein (GFP, 27 kDa) in Escherichia coli in the presence or absence of four coexpressed proteins: cytoplasmic maltose binding protein (42 kDa), tau-40 (45 kDa), alpha-synuclein (14 kDa), or calmodulin (17 kDa...


    pub_type: 杂志文章


    authors: Slade KM,Baker R,Chua M,Thompson NL,Pielak GJ

    更新日期:2009-06-16 00:00:00