Abstract：
:Synaptotagmin 1 (syt1) is a synaptic vesicle-anchored membrane protein that acts as the calcium sensor for the synchronous component of neuronal exocytosis. Using site-directed spin labeling, the position and membrane interactions of a fragment of syt1 containing its two C2 domains (syt1C2AB) were assessed in bilayers containing phosphatidylcholine (PC), phosphatidylserine (PS), and phosphatidylinositol 4,5-bisphosphate (PIP(2)). Addition of 1 mol % PIP(2) to a lipid mixture of PC and PS results in a deeper membrane penetration of the C2A domain and alters the orientation of the C2B domain so that the polybasic face of C2B comes into the proximity of the bilayer interface. The C2B domain is found to contact the membrane interface in two regions, the Ca(2+)-binding loops and a region opposite the Ca(2+)-binding loops. This suggests that syt1C2AB is configured to bridge two bilayers and is consistent with a model generated previously for syt1C2AB bound to membranes of PC and PS. Point-to-plane depth restraints, obtained by progressive power saturation, and interdomain distance restraints, obtained by double electron-electron resonance, were obtained in the presence of PIP(2) and used in a simulated annealing routine to dock syt1C2AB to two membrane interfaces. The results yield an average structure different from what is found in the absence of PIP(2) and indicate that bilayer-bilayer spacing is decreased in the presence of PIP(2). The results indicate that PIP(2), which is necessary for bilayer fusion, alters C2 domain orientation, enhances syt1-membrane electrostatic interactions, and acts to drive vesicle and cytoplasmic membrane surfaces closer together.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Kuo W,Herrick DZ,Cafiso DSdoi
10.1021/bi200049csubject
Has Abstractpub_date
2011-04-05 00:00:00pages
2633-41issue
13eissn
0006-2960issn
1520-4995journal_volume
50pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The three lipoyl (E2plip) domains of the dihydrolipoyl acetyltransferase component of the pyruvate dehydrogenase (PDH) complex of Escherichia coli house the lipoyl-lysine side chain essential for active-site coupling and substrate channelling within the complex. The structure of the unlipoylated form of the innermost ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi992978i
更新日期：2000-07-25 00:00:00
abstract：:The helical regions of RNA are generally very stable, but the single-stranded and loop regions often exist as an ensemble of conformations in solution. The theophylline-binding RNA aptamer forms a very stable structure when bound to the bronchodilator theophylline, but the theophylline binding site is not stably forme...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi027103+
更新日期：2003-03-11 00:00:00
abstract：:Type I repeat sequences are evolutionarily conserved sequence elements found in the replication origin and transcriptional promoter region of the rRNA genes (rDNA) in Tetrahymena thermophila. An abundant single-stranded DNA binding protein, ssA-TIBF, specifically interacts with the A-rich strand of the Type I repeat s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00014a011
更新日期：1995-04-11 00:00:00
abstract：:We recently introduced a new substrate rescue tool for investigating enzymatic base flipping by uracil DNA glycosylase (UDG) in which a bulky pyrene nucleotide wedge (Y) was placed opposite a uracil in duplex DNA (i.e., a U/Y pair), thereby preorganizing the target base in an extrahelical conformation [Jiang, Y. L., e...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi026227j
更新日期：2002-09-17 00:00:00
abstract：:cGMP is the natural activator of the cyclic nucleotide-gated channel originally isolated from rod photoreceptors but now known to be expressed in a wide variety of neural and non-neural cells. To identify antagonists of cGMP action and to better understand the interaction between cGMP and the channel protein, experime...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961763v
更新日期：1996-12-24 00:00:00
abstract：:The catalytic utilization of dimethylallyl, geranyl, farnesyl, and geranylgeranyl diphosphates in the reaction catalyzed by recombinant human farnesyl:protein transferase (hFPTase) has been examined in the presence of three different protein substrates, Ras-CVLS, Ras-CVIM, and Ras-CAIL. hFPTase catalyzed both farnesyl...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00083a038
更新日期：1993-08-17 00:00:00
abstract：:The interaction of the retroviral nucleocapsid protein (NC) with nucleic acids forms the basis of its varied roles in the replication cycle, which include binding and condensing the viral RNA within the virion, stimulation of the early steps in reverse transcription, and dissociation from RNA in the replication comple...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00126a020
更新日期：1992-03-24 00:00:00
abstract：:Protein arginine methyltransferases (PRMTs) catalyze the transfer of methyl groups from S-adenosylmethionine (SAM) to the guanidinium group of arginine residues in a number of important cell signaling proteins. PRMT1 is the founding member of this family, and its activity appears to be dysregulated in heart disease an...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi102022e
更新日期：2011-04-26 00:00:00
abstract：:Recent studies have shown that there can be large differences in the stability of double and triple helical nucleic acid complexes, depending on whether RNA or DNA strands are involved. These differences have been attributed to structural differences in the sugar-phosphate backbone of these two polymers. However, sinc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00012a031
更新日期：1995-03-28 00:00:00
abstract：:Ketosteroid isomerase (KSI) is one of the most proficient enzymes catalyzing an allylic isomerization reaction at a diffusion-controlled rate. In this study of KSI, we have detailed the structures of its active site, the role of various catalytic residues, and have explained the origin of the its fast reactivity by ca...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi001629h
更新日期：2000-11-14 00:00:00
abstract：:The lipid peroxidation product 4-hydroxynonenal (4-HNE) is a signaling mediator with wide-ranging biological effects. In this paper, we report that disruption of mGsta4, a gene encoding the 4-HNE-conjugating enzyme mGSTA4-4, causes increased 4-HNE tissue levels and is accompanied by age-dependent development of obesit...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi702124u
更新日期：2008-03-25 00:00:00
abstract：:The S-protein/vitronectin gene was isolated from a human genomic DNA library, and its sequence of about 5.3 kilobases including the adjacent 5' and 3' flanking regions was established. Alignment of the genomic DNA nucleotide sequence and the cDNA sequence indicated that the gene consisted of eight exons and seven intr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00395a024
更新日期：1987-10-20 00:00:00
abstract：:Human telomeres are comprised of d(TTAGGG) repeats that are capable of forming G-quadruplex DNA structures. Ligands that bind to and stabilize these G-quadruplex DNA structures are potential inhibitors of the cancer cell-associated enzyme telomerase. Other potential biological uses of G-quadruplex targeting ligands ha...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0263107
更新日期：2002-09-24 00:00:00
abstract：:The guanyl nucleotide binding regulatory protein of retinal rod outer segments, called Gt, that couples the photon receptor rhodopsin with the light-activated cGMP phosphodiesterase, can be resolved into two functional components, alpha t and beta gamma t. The effect of monoclonal antibody binding to the alpha t subun...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00451a047
更新日期：1989-12-12 00:00:00
abstract：:The kinetic and equilibrium CO binding properties of hemoglobin (Hb) M Iwate (alpha2 87 His leads to Tyr beta2) have been investigated. The results show that the alpha(Met) beta2 (CO) tetramer of this protein has a low affinity for CO, as indicated by the stopped-flow and flash-photolysis kinetic, as well as the CO bi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00669a022
更新日期：1976-11-30 00:00:00
abstract：:DREAM (also known as K(+) channel interacting protein 3 and calsenilin) is a calcium binding protein and an active modulator of KV4 channels in neuronal cells as well as a novel Ca(2+)-regulated transcriptional modulator. DREAM has also been associated with the regulation of Alzheimer's disease through the prevention ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00067
更新日期：2016-03-29 00:00:00
abstract：:In the nacre or aragonite layer of the mollusk shell, proteomes that regulate both the early stages of nucleation and nano-to-mesoscale assembly of nacre tablets from mineral nanoparticle precursors exist. Several approaches have been developed to understand protein-associated mechanisms of nacre formation, yet we sti...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00163
更新日期：2016-04-26 00:00:00
abstract：:Recombinant human brain calbindin D(28K) (rHCaBP), human Cu,Zn-superoxide dismutase (HCuZnSOD), rabbit muscle glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and bovine serum albumin (BSA) were found to be S-glutathiolated in decomposed S-nitrosoglutathione (GSNO) solutions. Tryptic or Glu-C digestion and MALDI-TOF ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi035924o
更新日期：2004-04-06 00:00:00
abstract：:Chemical and enzymatic probing (footprinting) of the reactivity of the promoter DNA backbone is applied to characterize two binary open complexes RPo1 (-Mg2+) and RPo2 (+Mg2+), formed by Escherichia coli RNA polymerase (E sigma 70) at the lambda PR promoter. We report that HO. detects major differences in backbone rea...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00048a004
更新日期：1995-12-05 00:00:00
abstract：:The prion protein (PrP) is a cell-surface Cu2+ binding glycoprotein which can bind six copper ions. The role of Cu2+ in PrP function, misfolding, and prion disease has generated much interest; however, the field has been hampered by a lack of consensus with regard to the affinity of Cu2+ for PrPC. Here we build on our...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9011397
更新日期：2009-09-29 00:00:00
abstract：:The intestinal fatty acid binding protein (IFABP) is a small (15 kDa) protein consisting mostly of 10 antiparallel beta-strands (A-J) and a small helical region that serves as a portal for the ligand. Two beta-sheet structures (strands A-E and F-J) surround a cavity into which the ligand binds. In this work, we invest...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0301688
更新日期：2003-10-28 00:00:00
abstract：:An attractive strategy for the development of anti-retroviral drugs is the exploration of compounds that mimic RNA control regions of the viral genome and act as "decoys" to sequester viral gene regulatory proteins. Decoys consisting of RNA, however, are chemically unstable and readily degraded by cellular nucleases. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961980w
更新日期：1997-01-21 00:00:00
abstract：:The electron spin resonance (ESR) spectra from spin-labeled phospholipids in recombinants of myelin proteolipid apoprotein with dimyristoylphosphatidylcholine have been simulated with the exchanged-coupled Bloch equations to obtain values for both the fraction of motionally restricted lipids and the exchange rate betw...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00401a009
更新日期：1988-01-12 00:00:00
abstract：:Amyloid diseases make up a set of fatal disorders in which proteins aggregate to form fibrils that deposit in tissues throughout the body. Amyloid-associated diseases are challenging to study because amyloid formation occurs on time scales that span several orders of magnitude and involve heterogeneous, interconvertin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.9b00769
更新日期：2019-11-05 00:00:00
abstract：:The kinetics and thermodynamics of Al(III) exchange between aluminum citrate (AlL) and human serum transferrin were investigated in the 7.2-8.9 pH range. The C-site of human serum apotransferrin in interaction with bicarbonate removes Al(III) from Al citrate with an exchange equilibrium constant K1 = (2.0 +/- 0.6) x 1...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi020627p
更新日期：2003-03-18 00:00:00
abstract：:Equilibrium binding experiments are widely used for the accurate characterization of binding and competitive binding behavior in biological systems. Modern high-throughput discovery efforts in chemical biology rely heavily upon this principle. Here, we derive exact analytical expressions for general competitive bindin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi048233g
更新日期：2004-12-28 00:00:00
abstract：:The requirement of cytochrome c-550 (PSII-V) in photosystem II (PSII) has been assessed in Synechocystis sp. PCC 6803 containing mutations between Gly-351 and Thr-436 of the loop E domain of the chlorophyll a-binding protein CP47. Six photoautotrophic strains were utilized to compare the effect of removal of either th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980404s
更新日期：1998-10-13 00:00:00
abstract：:Sequence-specific assignments of 1H NMR resonances are obtained for the backbone protons of Escherichia coli acyl carrier protein, acylated with an eight-carbon chain covalently attached to the prosthetic group thiol (octanoyl-ACP). Comparison of 1H-1H sequential connectivities in the NOESY spectra of octanoyl-ACP and...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00386a037
更新日期：1987-06-16 00:00:00
abstract：:The solution of a primary 16S rRNA-binding ribosomal protein, S17, was investigated by two- and three-dimensional homonuclear and heteronuclear magnetic resonance spectroscopy. Almost complete chemical shift assignments for the 1H, 15N, and 13C resonances have been obtained. The NMR data have been rigorously analyzed ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi951062i
更新日期：1996-03-05 00:00:00
abstract：:Enolase in the presence of Mg2+ catalyzes the elimination of H2O from 2-phosphoglyceric acid (PGA) to form phosphoenolpyruvate (PEP) and the reverse reaction, the hydration of PEP to PGA. The structure of the ternary complex yeast enolase-Mg2(+)-PGA/PEP has been determined by X-ray diffraction and refined by crystallo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00225a012
更新日期：1991-03-19 00:00:00
