Abstract：
:The biophysical analysis of multidomain proteins often is difficult because of overlapping signals from the individual domains. Previously, the fluorescent unnatural amino acid p-cyanophenylalanine has been used to study the folding of small single-domain proteins. Here we extend its use to a two-domain protein to selectively analyze the folding of a specific domain within a multidomain protein.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Ries LK,Schmid FX,Schmidpeter PAdoi
10.1021/acs.biochem.6b00898subject
Has Abstractpub_date
2016-12-13 00:00:00pages
6739-6742issue
49eissn
0006-2960issn
1520-4995journal_volume
55pub_type
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