Effect of protein hydration on receptor conformation: decreased levels of bound water promote metarhodopsin II formation.

abstract：:Coumermycin A(1) is a member of the aminocoumarin family of antibiotics. Unlike its structural relatives, novobiocin and clorobiocin, coumermycin A(1) is a dimer built on a 3-methyl-2,4-dicarboxypyrrole scaffold and bears two decorated noviose sugar components which are the putative target binding motifs for DNA gyras...

journal_title：Biochemistry

authors： Freel Meyers CL,Oberthür M,Heide L,Kahne D,Walsh CT

更新日期：2004-11-30 00:00:00

abstract：:Multiple intracellular receptors of the FK506 binding protein (FKBP) family of peptidylprolyl cis/trans-isomerases are potential targets for the immunosuppressive drug FK506. Inhibition of the protein phosphatase calcineurin (CaN), which has been implicated in the FK506-mediated blockade of T cell proliferation, was s...

journal_title：Biochemistry

authors： Weiwad M,Edlich F,Kilka S,Erdmann F,Jarczowski F,Dorn M,Moutty MC,Fischer G

更新日期：2006-12-26 00:00:00

abstract：:Ubiquitin was modified with the anionic, arginine-specific reagent 4-(oxoacetyl)phenoxyacetic acid in order to study the relationship between structure and function of the molecule. Four different derivatives (A, B, C, and D) were purified from the reaction mixture by anion-exchange high-performance liquid chromatogra...

journal_title：Biochemistry

authors： Duerksen-Hughes PJ,Xu XX,Wilkinson KD

更新日期：1987-11-03 00:00:00

abstract：:The major plasma membrane protein of human erythrocytes is the anion transport protein, termed protein 3. We previously reported a variant form of protein 3 that is elongated on the amino-terminal end of the molecule, which is exposed on the cytoplasmic side of the membrane, but otherwise its features are identical wi...

journal_title：Biochemistry

authors： Hsu L,Morrison M

更新日期：1985-06-18 00:00:00

abstract：:Previous studies have shown that protein 4.1 is a multifunctional protein that binds to spectrin, actin, glycophorins, the anion channel protein, and phosphatidylserine (PS). In this report, we have characterized the binding of protein 4.1 and its major proteolytic fragments to phospholipid vesicles. Pure 125I-labeled...

journal_title：Biochemistry

authors： Cohen AM,Liu SC,Lawler J,Derick L,Palek J

更新日期：1988-01-26 00:00:00

abstract：:When grown under O2-limited conditions, Escherichia coli expresses a cytochrome bd quinol oxidase that has an unusually high affinity for O2. We have studied the reaction of cytochrome bd with CO and O2 by rapid-reaction spectrophotometry. The reduced enzyme forms a photosensitive ferrocytochrome d-CO complex, and fol...

journal_title：Biochemistry

authors： Hill BC,Hill JJ,Gennis RB

更新日期：1994-12-20 00:00:00

abstract：:The electron density map of the 3D crystal of Photosystem II from Thermosynechococcus vulcanus with a 1.9 Å resolution (PDB: 3ARC ) exhibits, in the two monomers in the asymmetric unit cell, an, until now, unidentified and uninterpreted strong difference in electron density centered at a distance of around 1.5 Å from ...

journal_title：Biochemistry

authors： Sugiura M,Koyama K,Umena Y,Kawakami K,Shen JR,Kamiya N,Boussac A

更新日期：2013-12-31 00:00:00

abstract：:In experiments designed to characterize the basis of amyloid fibril stability through mutational analysis of the Abeta (1-40) molecule, fibrils exhibit consistent, significant structural malleability. In these results, and in other properties, amyloid fibrils appear to more resemble plastic materials generated from sy...

journal_title：Biochemistry

authors： Wetzel R,Shivaprasad S,Williams AD

更新日期：2007-01-09 00:00:00

abstract：:Decorin-binding protein A (DBPA) is an important lipoprotein from the bacterium Borrelia burgdorferi, the causative agent of Lyme disease. The absence of DBPA drastically reduces the pathogenic potential of the bacterium, and biochemical evidence indicates DBPA's interactions with the glycosaminoglycan (GAG) portion o...

journal_title：Biochemistry

authors： Wang X

更新日期：2012-10-23 00:00:00

abstract：:Phospholamban (PLB) is a major target of the beta-adrenergic cascade in the heart, and functions to modulate rate-limiting conformational transitions involving the transport activity of the Ca-ATPase. To investigate structural changes within the Ca-ATPase that result from the phosphorylation of PLB by cAMP-dependent p...

journal_title：Biochemistry

authors： Negash S,Huang S,Squier TC

更新日期：1999-06-22 00:00:00

abstract：:The kinetic mechanism of NADPH-cytochrome P450 oxidoreductase (P450R) has been determined for the model reaction with cytochrome c3+. Although initial velocity studies show parallel patterns, consistent with a classical (one-site) ping-pong mechanism that precludes the formation of a ternary NADPH-P450R-cytochrome c3+...

journal_title：Biochemistry

authors： Sem DS,Kasper CB

更新日期：1994-10-11 00:00:00

abstract：:Effects of paramagnetic shift reagents on the 13C NMR spectra obtained from single-walled vesicle dispersions of egg phosphatidylcholine enriched with 13C in the N-methyl carbons are investigated. Spectra obtained at 25.1 MHz show that, at Yb3+ to phospholipid molar ratios as low as 0.06, complete resolution of the N-...

journal_title：Biochemistry

authors： Sears B,Hutton WC,Thompson TE

更新日期：1976-04-20 00:00:00

abstract：:The DNA interstrand cross-link (ICL) resulting from the C4'-oxidized abasic site (C4-AP) is a unique clustered lesion comprised of a cross-link adjacent to a nick. The ICL is a substrate for the UvrABC nucleotide excision repair system. The strand containing the nick is preferentially incised, but the nick influences ...

journal_title：Biochemistry

authors： Sczepanski JT,Jacobs AC,Van Houten B,Greenberg MM

更新日期：2009-08-18 00:00:00

abstract：:Time-resolved decays of fluorescence anisotropy were obtained from frequency-domain measurements of the phase angle difference between the parallel and perpendicular components of the polarized emission and the ratio of the modulated amplitudes. These data were measured at modulation frequencies ranging from 1 to 200 ...

journal_title：Biochemistry

authors： Lakowicz JR,Cherek H,Maliwal BP

更新日期：1985-01-15 00:00:00

abstract：:S100 proteins are small dimeric members of the EF-hand superfamily of Ca(2+) binding proteins thought to participate in mediating intracellular Ca(2+) signals by binding to and thereby regulating target proteins in a Ca(2+)-dependent manner. As dimer formation is crucial to S100 function, we applied a yeast two-hybrid...

journal_title：Biochemistry

authors： Koltzscher M,Gerke V

更新日期：2000-08-08 00:00:00

abstract：:The catalysis of the chemical exchange between acetaldehyde and its hydrate by bovine carbonic anhydrase B was investigated by the NMR line broadening and saturation transfer techniques. While both chemical exchange and binding to the enzyme had an effect on the line broadening of acetaldehyde and its hydrate, the sat...

journal_title：Biochemistry

authors： Cheshnovsky D,Navon G

更新日期：1980-04-29 00:00:00

abstract：:C4'-oxidized (C4-AP) and C5'-oxidized abasic sites (DOB) that are produced following abstraction of a hydrogen atom from the DNA backbone reversibly form cross-links selectively with dA opposite a 3'-adjacent nucleotide, despite the comparable proximity of an opposing dA. A previous report on UvrABC incision of DNA su...

journal_title：Biochemistry

authors： Ghosh S,Greenberg MM

更新日期：2015-10-13 00:00:00

abstract：:Heat shock protein 90 (Hsp90) is a highly conserved ATP-driven machine involved in client protein maturation, folding, and activation. The chaperone is supported by a set of cochaperones that confer client specificities. One of those proteins is the suppressor of G2 allele of skp1 (Sgt1), which participates together w...

journal_title：Biochemistry

authors： Eckl JM,Drazic A,Rutz DA,Richter K

更新日期：2014-04-22 00:00:00

abstract：:New isoforms of CYP51 (sterol 14alpha-demethylase), an essential enzyme in sterol biosynthesis and primary target of azole antimycotic drugs, are found in pathogenic protists, Trypanosoma brucei(TB), T. vivax, T. cruzi, and Leishmania major. The sequences share approximately 80% amino acid identity and are approximate...

journal_title：Biochemistry

authors： Lepesheva GI,Nes WD,Zhou W,Hill GC,Waterman MR

更新日期：2004-08-24 00:00:00

abstract：:To better understand how amino acid sequences specify unique tertiary folds, we have used random mutagenesis and phage display selection to evolve proteins with a high degree of sequence identity but different tertiary structures (homologous heteromorphs). The starting proteins in this evolutionary process were the Ig...

journal_title：Biochemistry

authors： Alexander PA,Rozak DA,Orban J,Bryan PN

更新日期：2005-11-01 00:00:00

abstract：:Structures of several voltage-gated sodium (NaV) channels from bacteria have been determined recently, but the same feat might not be achieved for the mammalian counterparts in the near future. Thus, at present, computational studies of the mammalian NaV channels have to be performed using homology models based on the...

journal_title：Biochemistry

authors： Patel D,Mahdavi S,Kuyucak S

更新日期：2016-03-29 00:00:00

abstract：:Genetic studies have previously demonstrated that the Saccharomyces cerevisiae CDC9 gene product, which is functionally homologous to mammalian DNA ligase I, is required for DNA replication and is also involved in DNA repair and genetic recombination. In the present study we have purified the yeast enzyme. When measur...

journal_title：Biochemistry

authors： Tomkinson AE,Tappe NJ,Friedberg EC

更新日期：1992-12-01 00:00:00

abstract：:The potential of the frequently encountered (βα)8-barrel fold to acquire new functions was tested by an approach combining random mutagenesis and selection in vivo. For this purpose, the genes encoding 52 different phosphate-binding (βα)8-barrel proteins were subjected to error-prone PCR and cloned into an expression ...

journal_title：Biochemistry

authors： Rohweder B,Lehmann G,Eichner N,Polen T,Rajendran C,Ruperti F,Linde M,Treiber T,Jung O,Dettmer K,Meister G,Bott M,Gronwald W,Sterner R

更新日期：2019-10-15 00:00:00

abstract：:Tombusviruses use a 19 kDa protein (p19) as a suppressor of the RNA silencing pathway during infection. The p19 protein binds to short-interfering RNA (siRNA) as a dimer and shows a high selectivity for short duplex RNAs over other RNA species. Since p19 can bind to synthetic and RNA silencing generated small RNAs wit...

journal_title：Biochemistry

authors： Cheng J,Sagan SM,Jakubek ZJ,Pezacki JP

更新日期：2008-08-05 00:00:00

abstract：:Isopenicillin N synthase (IPNS) catalyzes the oxidative cyclization of delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valine to isopenicillin N. It is proposed that the multiple products produced from certain substrate analogues result from pathway branching after formation of a ferryl oxene intermediate. We have been int...

journal_title：Biochemistry

authors： Blackburn JM,Sutherland JD,Baldwin JE

更新日期：1995-06-06 00:00:00

abstract：:Membrane fusion is involved in many fundamental cellular processes and entry of enveloped viruses into host cells. Influenza type A virus HA has long served as a paradigm for mechanistic studies of protein-mediated membrane fusion via large-scale structural rearrangements induced by acidic pH. Here we report the newly...

journal_title：Biochemistry

authors： Ni F,Chen X,Shen J,Wang Q

更新日期：2014-02-11 00:00:00

abstract：:Thermodynamics of 39 oligonucleotides with internal G.T mismatches dissolved in 1 M NaCl were determined from UV absorbance versus temperature profiles. These data were combined with literature values of six sequences to derive parameters for 10 linearly independent trimer and tetramer sequences with G.T mismatches an...

journal_title：Biochemistry

authors： Allawi HT,SantaLucia J Jr

更新日期：1997-08-26 00:00:00

abstract：:Detailed information on room-temperature structure and oxidation state of the Photosystem II (PS II) manganese complex is needed to put mechanistic considerations on solid grounds. Because previously this information had not been available, the tetranuclear manganese complex was investigated by X-ray absorption spectr...

journal_title：Biochemistry

authors： Meinke C,Solé VA,Pospisil P,Dau H

更新日期：2000-06-20 00:00:00

abstract：:A covalently linked actin dimer is identified in solutions of actin prepared from an acetone powder from skeletal muscle. This actin dimer acts as an actin nucleating factor (ANF), decreasing the half-time for spontaneous actin polymerization. ANF reacts with antibodies to both the N- and C-terminal portions of actin ...

journal_title：Biochemistry

authors： Selden LA,Kinosian HJ,Estes JE,Gershman LC

更新日期：2000-01-11 00:00:00

abstract：:Multivalent binding is an efficient means to enhance the affinity and specificity of chemical probes targeting multidomain proteins in order to study their function and role in disease. While the theory of multivalent binding is straightforward, physical and structural characterization of bivalent binding encounters m...

journal_title：Biochemistry

authors： Suh JL,Watts B,Stuckey JI,Norris-Drouin JL,Cholensky SH,Dickson BM,An Y,Mathea S,Salah E,Knapp S,Khan A,Adams AT,Strahl BD,Sagum CA,Bedford MT,James LI,Kireev DB,Frye SV

更新日期：2018-04-10 00:00:00