Abstract：
:Genetic studies have previously demonstrated that the Saccharomyces cerevisiae CDC9 gene product, which is functionally homologous to mammalian DNA ligase I, is required for DNA replication and is also involved in DNA repair and genetic recombination. In the present study we have purified the yeast enzyme. When measured under denaturing conditions, Cdc9 protein has a polypeptide molecular mass of 87 kDa. The native form of the enzyme is an 80-kDa asymmetric monomer. Both estimates are in good agreement with the M(r) = 84,406 predicted from the translated sequence of the CDC9 gene. Cdc9 DNA ligase acts via the same basic reaction mechanism employed by all known ATP-dependent DNA ligases. The catalytic functions reside in a 70-kDa C-terminal domain that is conserved in mammalian DNA ligase I and in Cdc17 DNA ligase from Schizosaccharomyces pombe. The ATP analog ATP alpha S inhibits the ligation reaction, although Cdc9 protein does form an enzyme-thioadenylate intermediate. Since Cdc9 DNA ligase exhibited the same substrate specificity as mammalian DNA ligase I, this enzyme can be considered to be the DNA ligase I of S. cerevisiae. There is genetic evidence suggesting that DNA ligase may be directly involved in error-prone DNA repair. We examined the ability of Cdc9 DNA ligase to join nicks with mismatches at the termini. Mismatches at the 5' termini of nicks had very little effect on ligation, whereas mismatches opposite a purine at 3' termini inhibited DNA ligation. The joining of DNA molecules with mismatched termini by DNA ligase may be responsible for the generation of mutations.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Tomkinson AE,Tappe NJ,Friedberg ECdoi
10.1021/bi00162a013subject
Has Abstractpub_date
1992-12-01 00:00:00pages
11762-71issue
47eissn
0006-2960issn
1520-4995journal_volume
31pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Guanine nucleotide dissociation stimulator (GDS) promotes the release of tightly bound GDP from various Ras superfamily proteins, including RhoA, Rac1, K-Ras, Rap1A, and Rap1B. It displays no significant sequence homology to other known exchange factors for small G-proteins. Studies are reported here of the mechanism ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0007573
更新日期：2000-09-19 00:00:00
abstract：:The interaction of nile red (NR) with apomyoglobin (ApoMb) in the native (pH 7) and molten globule (pH 4) states was investigated using experimental and computational methods. NR binds to hydrophobic locations in ApoMb with higher affinity (K(d) = 25 +/- 5 microM) in the native state than in the molten globule state (...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051905y
更新日期：2006-04-25 00:00:00
abstract：:31P nuclear magnetic resonance (NMR) saturation-transfer (ST) techniques have been used to measure steady-state flows through phosphate-adenosine 5'-triphosphate (ATP) exchange reactions in glucose-grown derepressed yeast. Our results have revealed that the reactions catalyzed by glyceraldehyde-3-phosphate dehydrogena...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00397a043
更新日期：1987-11-17 00:00:00
abstract：:Stimulation of isolated hepatocytes with epidermal growth factor (EGF) causes rapid tyrosine phosphorylation of the EGF receptor (EGFR) and adapter/target proteins, which was monitored with 1 and 2 s resolution at 37, 20, and 4 degrees C. The temporal responses detected for multiple signaling proteins involve both tra...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011506c
更新日期：2002-01-08 00:00:00
abstract：:The functional role of distinct phospholipid subclasses and molecular species in modulating gramicidin-mediated K+ flux was characterized through quantification of changes in the fluorescence intensity of ion specific fluorescent probes encapsulated inside vesicles comprised of individual molecular species of plasmeny...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00022a008
更新日期：1995-06-06 00:00:00
abstract：:The relationship between GTP hydrolysis and microtubule assembly has been investigated by using a rapid filtration method. Microtubules assembled from phosphocellulose-purified tubulin, double-labeled with [gamma-32P]- and [3H]GTP, were trapped and washed free of unbound nucleotide on glass fiber filters. The transien...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00479a022
更新日期：1990-07-10 00:00:00
abstract：:Guanidinium chloride- (GdmCl-) induced unfolding of beta-lactamase has been investigated by a combination of size-exclusion chromatography (SEC-FPLC) and usual optical methods. It has been shown that at low temperatures this protein unfolds through two equilibrium intermediates. The first of these intermediates is the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00176a006
更新日期：1994-03-15 00:00:00
abstract：:Kinetics studies of dNTP analogues having pyrophosphate-mimicking β,γ-pCXYp leaving groups with variable X and Y substitution reveal striking differences in the chemical transition-state energy for DNA polymerase β that depend on all aspects of base-pairing configurations, including whether the incoming dNTP is a puri...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi500101z
更新日期：2014-03-25 00:00:00
abstract：:On the basis of the available high-resolution structures of mandelate racemase (MR) from Pseudomonas putida [Landro, J. A., Gerlt, J. A., Kozarich, J. W., Koo, C. W., Shah, V. J., Kenyon, G. L., Neidhart, D. J., Fujita, J., & Petsko, G. A. (1994) Biochemistry 33, 635-643], Lys 166 and His 297 are positioned appropriat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00009a007
更新日期：1995-03-07 00:00:00
abstract：:Recently, it has been shown that the activation of the Drosophila EGF receptor (DER) by its natural ligand Spitz is inhibited by Argos [Schweitzer, R., et al. (1995) Nature 376, 699-702]. Argos and Spitz both have an EGF-like domain which in the case of Argos differs from that of Spitz and other EGF receptor agonists ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970227f
更新日期：1997-06-17 00:00:00
abstract：:The ability of DNA topoisomerase I to mediate the formation of structurally modified DNA duplexes was studied utilizing suicide substrates containing high-efficiency cleavage sites and acceptor oligonucleotides in which the 5'-terminal nucleotides were varied. When the substrates were nicked duplexes, the divalent cat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972707c
更新日期：1998-02-24 00:00:00
abstract：:Although the synthesis of RNA from a DNA template is (and must be) a generally very stable process to enable transcription of kilobase transcripts, it has long been known that during initial transcription of the first 8-10 bases of RNA complexes are relatively unstable, leading to the release of short abortive RNA tra...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200620q
更新日期：2011-08-16 00:00:00
abstract：:The effects of ions on the interaction of lac repressor protein and operator DNA have been studied by the membrane filter technique. The equilibrium association constant was determined as a function of monovalent and divalent cation concentrations, anions, and pH. The binding of repressor and operator is extremely sen...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00516a027
更新日期：1981-06-23 00:00:00
abstract：:A five-residue loop structure in basic fibroblast growth factor (FGF-2) which extends from amino acid residue 118 to residue 122 was replaced, by cassette mutagenesis, with the corresponding seven-residue loop structure from the structural homologue acidic fibroblast growth factor (FGF-1) or the corresponding five-res...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00003a004
更新日期：1995-01-24 00:00:00
abstract：:Characterization of the complete gene sequence encoding the alpha-galactosidase from Phanerochaete chrysosporium confirms that this enzyme is a member of glycosyl hydrolase family 27 [Henrissat, B., and Bairoch, A. (1996) Biochem. J. 316, 695-696]. This family, together with the family 36 alpha-galactosidases, forms g...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0008074
更新日期：2000-08-15 00:00:00
abstract：:In bacteria, the transcription of virulence genes is usually controlled by a cell density-dependent process known as "quorum sensing" (QS). QS relies on small diffusible signaling molecules that cross the bacterial cell wall and activate target transcription factors after a threshold concentration has been reached. Be...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi900123j
更新日期：2009-11-03 00:00:00
abstract：:We report here that polyamide nucleic acid (PNA) as well as a polyamide nucleic acid-DNA chimera complementary to the primer binding site of the HIV-1 genome can completely block priming by tRNA3Lys and consequently the in vitro initiation of reverse transcription by HIV-1 RT. Conventional heating and cooling is not r...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972197m
更新日期：1998-01-20 00:00:00
abstract：:Streptococcus pneumoniae 5'-methylthioadenosine/S-adenosylhomocysteine hydrolase (MTAN) catalyzes the hydrolytic deadenylation of its substrates to form adenine and 5-methylthioribose or S-ribosylhomocysteine (SRH). MTAN is not found in mammals but is involved in bacterial quorum sensing. MTAN gene disruption affects ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi061184i
更新日期：2006-10-31 00:00:00
abstract：:The MexA-MexB-OprM efflux pump exports structurally and functionally diverse xenobiotics and confers multi-drug resistance on Pseudomonas aeruginosa cells. The MexB transporter traverses the inner membrane twelve times, bears two large periplasmic domains and has two homologous tandem repeats. To test whether two homo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0300074
更新日期：2003-06-17 00:00:00
abstract：:Tyramine β-monooxygenase (TβM), the insect homologue of dopamine β-monooxygenase, is a neuroregulatory enzyme that catalyzes the β-hydroxylation of tyramine to yield octopamine. Mutation of the methionine (Met) ligand to Cu(M) of TβM, Met471Cys, yielded a form of TβM that is catalytically active but susceptible to ina...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300456f
更新日期：2012-09-25 00:00:00
abstract：:The interaction of lipoprotein lipase (LpL) and a nonhydrolyzable phosphatidylcholine, 1,2-ditetradecyl-rac-glycero-3-phosphocholine (C14-ether-PC), has been studied by several physical methods. Analysis of the circular dichroic spectrum of LpL gave the following fractional conformation: 35% alpha-helix, 30% beta-plea...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00352a020
更新日期：1986-02-25 00:00:00
abstract：:S-Adenosylmethionine decarboxylase, a pyruvoyl-containing decarboxylase, is inactivated in a time-dependent process under turnover conditions. The inactivation is dependent on the presence of both substrate and Mg2+, which is also required for enzyme activity. The rate of inactivation is dependent on the concentration...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00394a022
更新日期：1987-10-06 00:00:00
abstract：:Murine cDNA that encodes neuromodulin, a neurospecific calmodulin binding protein, was inserted into the plasmid pKK223-3 for expression in Escherichia coli. After being transformed into E. coli strain SG20252 (lon-), the expression vector directed the synthesis of a protein that was recognized by polyclonal antibodie...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00446a027
更新日期：1989-10-03 00:00:00
abstract：:Furanacryloyl-Phe-Gly-Gly has been shown to be a convenient substrate for angiotensin converting enzyme (dipeptidyl carboxypeptidase, EC 3.4.15.1). A detailed kinetic analysis of the hydrolysis of this substrate indicates normal Michaelis-Menten behavior with kcat = 19000 min-1 and KM = 3.0 x 10(-4) M determined at pH...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00270a015
更新日期：1983-01-04 00:00:00
abstract：:Triglyceride analogues were synthesized in which one of the primary acyl ester functions has been replaced by an alkyl group and the secondary acyl ester bond has been replaced by an acyl amino bond. The chain length at either position was varied, and both (R)- and (S)-enantiomers of each compound were synthesized. Th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00019a020
更新日期：1995-05-16 00:00:00
abstract：:The argument and experimental evidence are presented for a two-state model that explains the action of both helical and beta-sheet antimicrobial peptides after they bind to the plasma membranes of cells. Each peptide has two distinct physical states of binding to lipid bilayers. At low peptide-to-lipid ratios (P/L), t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi000946l
更新日期：2000-07-25 00:00:00
abstract：:The first indication of the size of a conformational change implicated in ion transport by sodium pump has been obtained by measuring the change in efficiency of fluorescence energy transfer between two specific locations on the alpha-subunit. The donor (5'-(iodoacetamido)fluorescein) attaches covalently to cysteine-4...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi960407+
更新日期：1996-06-25 00:00:00
abstract：:Enterokinase has been isolated from the contents of bovine duodena and purified 1200-fold in 41% yield. The isolation procedure employed DEAE chromatography, affinity chromatography on immobilized p-aminobenzamidine, and gel filtration. The resultant pure enzyme exhibits a single band on sodium dodecyl sulfate gel ele...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00634a011
更新日期：1977-07-26 00:00:00
abstract：:Indolamine N-methyltransferase (INMT) has been purified to an apparent homogeneity from rabbit lung, and some of its catalytic and physicochemical properties have been examined. The enzyme is a monomeric protein with a molecular weight of 31,500 +/- 1000, a molecular Stokes radius of 21.5 A, and a diffusion coefficien...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00535a054
更新日期：1982-03-16 00:00:00
abstract：:The interactions of a series of spin-labeled fatty acids, in which the nitroxide ring is incorporated in different ways as an integral part of the hydrocarbon chain, with the (Na+,K+)-ATPase in membranes from Squalus acanthias, have been studied by electron spin resonance spectroscopy. The fatty acids are 2,4-, 2,5-, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00411a004
更新日期：1988-05-31 00:00:00
