31P NMR saturation-transfer measurements in Saccharomyces cerevisiae: characterization of phosphate exchange reactions by iodoacetate and antimycin A inhibition.

abstract：:Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (EC 5.3.3.2) catalyzes the antarafacial [1.3] allylic rearrangement of isopentenyl diphosphate (IPP) to its electrophilic allylic isomer dimethylallyl diphosphate (DMAPP). Active-site thiols at C138 and C139 were recently identified by covalent modification u...

journal_title：Biochemistry

authors： Street IP,Coffman HR,Baker JA,Poulter CD

更新日期：1994-04-12 00:00:00

abstract：:IIIGlc is an 18.1-kDa signal-transducing phosphocarrier protein of the phosphoenolpyruvate:glycose phosphotransferase system (PTS) of Escherichia coli. Virtually complete (98%) backbone 1H, 15N, and 13C nuclear magnetic resonance (NMR) signal assignments were determined by using a battery of triple-resonance three-dim...

journal_title：Biochemistry

authors： Pelton JG,Torchia DA,Meadow ND,Wong CY,Roseman S

更新日期：1991-10-15 00:00:00

abstract：:Following ribonuclease digestion of methyl-3H-labeled B77 avian sarcoma virus RNA subunits, methylated oligonucleotides were isolated by diethylaminoethylcellulose chromotogrpahy. Partial nucleotide sequences were deduced from the known enzymatic specificities of the ribonucleases. In addition to methylated nucleoside...

journal_title：Biochemistry

authors： Dimock K,Stoltzfus CM

更新日期：1977-02-08 00:00:00

abstract：:All phosphagen kinases contain a conserved cysteine residue which has been shown by crystallographic studies, on both creatine kinase and arginine kinase, to be located in the active site. There are conflicting reports as to whether this cysteine is essential for catalysis. In this study we have used site-directed mut...

journal_title：Biochemistry

authors： Wang PF,McLeish MJ,Kneen MM,Lee G,Kenyon GL

更新日期：2001-10-02 00:00:00

abstract：:Cathepsin C, or dipeptidyl peptidase I, is a lysosomal cysteine protease of the papain family that catalyzes the sequential removal of dipeptides from the free N-termini of proteins and peptides. Using the dipeptide substrate Ser-Tyr-AMC, cathepsin C was characterized in both steady-state and pre-steady-state kinetic ...

journal_title：Biochemistry

authors： Schneck JL,Villa JP,McDevitt P,McQueney MS,Thrall SH,Meek TD

更新日期：2008-08-19 00:00:00

abstract：:The red pigments in meat products, including cooked cured ham, arise from the reaction of myoglobin with nitric oxide generated from exogenous nitrite. Since carcinogenic nitrosoamines may be generated by the treatment of meats with nitrite, the production of nitrite-free meat products is an attractive alternative. Ra...

journal_title：Biochemistry

authors： Taketani S,Ishigaki M,Mizutani A,Uebayashi M,Numata M,Ohgari Y,Kitajima S

更新日期：2007-12-25 00:00:00

abstract：:Previous experiments have shown that a Synechocystis sp. PCC 6803 mutant (delta psbO) lacking the extrinsic manganese-stabilizing protein (MSP) exhibits impaired, but significant levels of H2O-splitting activity [Burnap, R., & Sherman, L.A. (1991) Biochemistry 30, 440-446]. [14C]DCMU-binding experiments now show that ...

journal_title：Biochemistry

authors： Burnap RL,Shen JR,Jursinic PA,Inoue Y,Sherman LA

更新日期：1992-08-18 00:00:00

abstract：:A newly recognized type of protein-ligand interaction phenomenon has resulted in the preparation of simple, nonionic, and highly specific gel derivatives for selective adsorption chromatography. The essential structure of the immobilized ligand can be represented as agarose-CH2CH2SO2CH2CH2SCH2CH2OH, which was prepared...

journal_title：Biochemistry

authors： Hutchens TW,Porath J

更新日期：1987-11-03 00:00:00

abstract：:Aromatic amino acid aminotransferase (ArAT) from Escherichia coli was overexpressed in E. coli cells, purified, and characterized. The enzyme was similar to aspartate aminotransferase (AspAT) of E. coli in many aspects, such as gross protein structure and spectroscopic properties. The reactions of pyridoxal 5'-phospha...

journal_title：Biochemistry

authors： Hayashi H,Inoue K,Nagata T,Kuramitsu S,Kagamiyama H

更新日期：1993-11-16 00:00:00

abstract：:Escherichia coli DNA photolyase mediates photorepair of pyrimidine dimers occurring in UV-damaged DNA. The enzyme contains two chromophores, 1,5-dihydroflavin adenine dinucleotide (FADH2) and 5,10-methenyltetrahydrofolylpolyglutamate (MTHF). To define the roles of the two chromophores in the photochemical reaction(s) ...

journal_title：Biochemistry

authors： Payne G,Sancar A

更新日期：1990-08-21 00:00:00

abstract：:The cytokinin-active isoprenoid nucleosides of Agrobacterium tumefaciens transfer ribonucleic acid were identified by high-pressure liquid chromatography, permethylation, and mass spectroscopy. Besides the expected 6-[(3-methylbut-2-enyl)amino]-9-(beta-D-ribofuranosyl)purine (i6A) and its 2-methylthio derivative (ms2i...

journal_title：Biochemistry

authors： Morris RO,Regier DA,Olson RM Jr,Struxness LA,Armstrong DJ

更新日期：1981-10-13 00:00:00

abstract：:Inheritance of apoE4 is a strong risk factor for the development of late-onset sporadic Alzheimer's disease (AD). Several lines of evidence suggest that apoE4 binds to the Alzheimer Abeta protein and, under certain experimental conditions, promotes formation of beta-sheet structures and amyloid fibrils. Deposition of ...

journal_title：Biochemistry

authors： Poeggeler B,Miravalle L,Zagorski MG,Wisniewski T,Chyan YJ,Zhang Y,Shao H,Bryant-Thomas T,Vidal R,Frangione B,Ghiso J,Pappolla MA

更新日期：2001-12-11 00:00:00

abstract：:The signaling apparatus that controls bacterial chemotaxis is composed of a core complex containing chemoreceptors, the histidine autokinase CheA, and the coupling protein CheW. Site-specific spin labeling and pulsed dipolar ESR spectroscopy (PDS) have been applied to investigate the structure of a soluble ternary com...

journal_title：Biochemistry

authors： Bhatnagar J,Borbat PP,Pollard AM,Bilwes AM,Freed JH,Crane BR

更新日期：2010-05-11 00:00:00

abstract：:Sequence-specific DNA binding proteins generally consist of more than two DNA-contacting regions to ensure the selectivity of recognition. The multiple DNA binding modules are connected either through the covalent linker or through the noncovalent dimerization domain. We have compared the DNA binding of peptide dimers...

journal_title：Biochemistry

authors： Aizawa Y,Sugiura Y,Morii T

更新日期：1999-02-02 00:00:00

abstract：:This paper describes the effect of N-capping substitutions on the structure and stability of histidine-containing protein (HPr). We have used NMR spectroscopy and conformational stability studies to quantify changes in local and global free energy due to mutagenesis at Ser46, the N-cap for helix B in HPr. Previous NMR...

journal_title：Biochemistry

authors： Thapar R,Nicholson EM,Rajagopal P,Waygood EB,Scholtz JM,Klevit RE

更新日期：1996-09-03 00:00:00

abstract：:S100A6 is a member of the S100 subfamily of EF-hand Ca (2+) binding proteins that has been shown to interact with calcyclin binding protein/Siah-1 interacting protein (CacyBP/SIP or SIP), a subunit of an SCF-like E3 ubiquitin ligase complex (SCF-TBL1) formed under genotoxic stress. SIP serves as a scaffold in this com...

journal_title：Biochemistry

authors： Lee YT,Dimitrova YN,Schneider G,Ridenour WB,Bhattacharya S,Soss SE,Caprioli RM,Filipek A,Chazin WJ

更新日期：2008-10-14 00:00:00

abstract：:Proteolytic fragments of bovine testis calmodulin were obtained by limited proteolysis with trypsin or thrombin. Cadmium-113 NMR studies showed that the tryptic fragment encompassing Ca2+ binding domains III and IV (TR2C) gave rise to a spectrum identical with that of the native protein. Two thrombic fragments contain...

journal_title：Biochemistry

authors： Andersson A,Forsén S,Thulin E,Vogel HJ

更新日期：1983-05-10 00:00:00

abstract：:Electron paramagnetic resonance (EPR) at 15 K was used to probe the magnetic interaction between the visible copper CuA2+ and ferric cytochrome a in the carbon monoxide compound of beef heart cytochrome oxidase. At pH 8.6, the midpoint potentials (Em's) for one-electron oxidation of CuA+ and cytochrome a2+ were found ...

journal_title：Biochemistry

authors： Goodman G,Leigh JS Jr

更新日期：1985-04-23 00:00:00

abstract：:Myotoxin a from the venom of Crotalus viridis viridis (prairie rattlesnake) is a small protein which is responsible for myonecrosis. It is a basic protein with 42 amino acid residues of known sequence. Three disulfide bonds give it a highly compact structure. Microscopic examination of the toxin's effects reveals that...

journal_title：Biochemistry

authors： Liddle WK,Tu AT

更新日期：1985-12-17 00:00:00

abstract：:The isothermal equilibrium folding of the unmodified yeast tRNA(Phe) is studied as a function of Na(+), Mg(2+), and urea concentration with hydroxyl radical protection, circular dichroism, and diethyl pyrocarbonate (DEPC) modification. These assays indicate that this tRNA folds in Na(+) alone. Similar to folding in Mg...

journal_title：Biochemistry

authors： Shelton VM,Sosnick TR,Pan T

更新日期：2001-03-27 00:00:00

abstract：:Ppr from the purple phototrophic bacterium, Rhodospirillum centenum (also known as Rhodocista centenaria), is a hybrid of photoactive yellow protein (PYP), bacteriophytochrome (Bph), and histidine kinase (HK) domains. The holo-Ppr (containing both chromophores) exhibits characteristic absorption maxima at 435 nm due t...

journal_title：Biochemistry

authors： Kyndt JA,Fitch JC,Meyer TE,Cusanovich MA

更新日期：2007-07-17 00:00:00

abstract：:Cell-free extracts from the HTl clone of cultured Chinese hamster lung cells efficiently promote the incorporation of proline into newly synthesized material, 50% of which is digestible to small peptides by highly purified bacterial collagenase. The synthesis of the these products occurs under optimal protein synthesi...

journal_title：Biochemistry

authors： Haralson MA,Frey KL,Mitchell WM

更新日期：1978-03-07 00:00:00

abstract：:ATP-binding cassette (ABC) transporters are ubiquitous in all three kingdoms of life and are implicated in many clinically relevant physiological processes. They couple the energy released by ATP hydrolysis to facilitate substrate translocation across cell membranes. The crystal structures of type II ABC importers hav...

journal_title：Biochemistry

authors： Pan C,Weng J,Wang W

更新日期：2016-12-13 00:00:00

abstract：:The development of inhibitors of insulin-regulated aminopeptidase (IRAP), a membrane-bound zinc metallopeptidase, is a promising approach for the discovery of drugs for the treatment of memory loss such as that associated with Alzheimer's disease. There is, however, no consensus in the literature about the mechanism b...

journal_title：Biochemistry

authors： Ascher DB,Cromer BA,Morton CJ,Volitakis I,Cherny RA,Albiston AL,Chai SY,Parker MW

更新日期：2011-04-05 00:00:00

abstract：:Two major neutral glyceroglucolipids (A and B) have been isolated from lipid extract of human gastric content by the procedure involving column fractionation on DEAE-Sephadex, silicic acid, and thin-layer chromatography. Both glycolipids contained glucose, glyceryl ethers, and fatty acids. The structures of these glyc...

journal_title：Biochemistry

authors： Slomiany BL,Slomiany A,Glass GB

更新日期：1977-09-06 00:00:00

abstract：:Pepstatin is a slow and tight-binding inhibitor of pepsin. Preincubating enzyme and inhibitor in H2O and in D2O in the absence of substrate generates an inverse solvent isotope effect of Dk = 0.69 +/- 0.06 on the apparent first-order rate constant for the decay in enzymatic activity. Proton inventory analysis of the i...

journal_title：Biochemistry

authors： Cho YK,Rebholz KL,Northrop DB

更新日期：1994-08-16 00:00:00

abstract：:Mixtures of dimyristoylphosphatidylcholine (DMPC) and cholesterol (Chol) have been used to examine the effects of cholesterol on the chain crystallization transitions and thermomechanical properties in phospholipid bilayer membranes. The mechanical properties--elastic moduli and level of tension at membrane rupture--w...

journal_title：Biochemistry

authors： Needham D,McIntosh TJ,Evans E

更新日期：1988-06-28 00:00:00

abstract：:DNA sequences with repetitive G-rich structural motifs, which form special structures called G-quadruplexes, widely exist in the human genome. Here we report the general peroxidase activity of G-quadruplex-hemin complexes and discuss the connection between peroxidase activity and G-quadruplex structures. The high pero...

journal_title：Biochemistry

authors： Cheng X,Liu X,Bing T,Cao Z,Shangguan D

更新日期：2009-08-25 00:00:00

abstract：:The crystal structure of a C-terminal domain of enterohemorrhagic Escherichia coli type III effector NleH2 has been determined to 2.6 Å resolution. The structure resembles those of protein kinases featuring the catalytic, activation, and glycine-rich loop motifs and ATP-binding site. The position of helix αC and the l...

journal_title：Biochemistry

authors： Halavaty AS,Anderson SM,Wawrzak Z,Kudritska M,Skarina T,Anderson WF,Savchenko A

更新日期：2014-04-22 00:00:00

abstract：:The ferritin IRE, a highly conserved (96-99% in vertebrates) mRNA translation regulatory element in animal mRNA, was studied by molecular modeling (using MC-SYM and DOCKING) and by NMR spectroscopy. Cobalt(III) hexammine was used to model hydrated Mg2+. IRE isoforms in other mRNAs regulate mRNA translation or stabilit...

journal_title：Biochemistry

authors： Gdaniec Z,Sierzputowska-Gracz H,Theil EC

更新日期：1998-02-10 00:00:00