Abstract：
:IIIGlc is an 18.1-kDa signal-transducing phosphocarrier protein of the phosphoenolpyruvate:glycose phosphotransferase system (PTS) of Escherichia coli. Virtually complete (98%) backbone 1H, 15N, and 13C nuclear magnetic resonance (NMR) signal assignments were determined by using a battery of triple-resonance three-dimensional (3D) NMR pulse sequences. In addition, nearly complete (1H, 95%; 13C, 85%) side-chain 1H and 13C signal assignments were obtained from an analysis of 3D 13C HCCH-COSY and HCCH-TOCSY spectra. These experiments rely almost exclusively upon one- and two-bond J couplings to transfer magnetization and to correlate proton and heteronuclear NMR signals. Hence, essentially complete signal assignments of this 168-residue protein were made without any assumptions regarding secondary structure and without the aid of a crystal structure, which is not yet available. Moreover, only three samples, one uniformly 15N-enriched, one uniformly 15N/13C-enriched, and one containing a few types of amino acids labeled with 15N and/or 13C, were needed to make the assignments. The backbone assignments together with the 3D 15N NOESY-HMQC and 13C NOESY-HMQC data have provided extensive information about the secondary structure of this protein [Pelton, J.G., Torchia, D.A., Meadow, N.D., Wong, C.-Y., & Roseman, S (1991) Proc. Natl. Acad. Sci. U.S.A. 88, 3479-3488]. The nearly complete set of backbone and side-chain atom assignments reported herein provide a basis for studies of the three-dimensional structure and dynamics of IIIGlc as well as its interactions with a variety of membrane and cytoplasmic proteins.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Pelton JG,Torchia DA,Meadow ND,Wong CY,Roseman Sdoi
10.1021/bi00105a032subject
Has Abstractpub_date
1991-10-15 00:00:00pages
10043-57issue
41eissn
0006-2960issn
1520-4995journal_volume
30pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Biotin synthase (BioB) is an iron-sulfur dimeric enzyme which catalyzes the last step in biotin synthesis. The reaction consists of the introduction of a sulfur atom into dethiobiotin. It is shown here that BioB displays a significant cysteine desulfurase activity, providing it with the ability to mobilize sulfur from...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0122011
更新日期：2002-07-23 00:00:00
abstract：:The PLL(PTE-like lactonase)-group of enzymes within the amidohydrolase superfamily hydrolyze N-acyl-homoserine lactones (AHLs) that are involved in bacterial quorum-sensing pathways. These enzymes possess the (beta/alpha)(8)-barrel fold and serve as attractive templates for in vitro evolution and engineering of quorum...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9004045
更新日期：2009-05-26 00:00:00
abstract：:Here we present a biophysical, structural, and computational analysis of the directed evolution of the human DNA repair protein O(6)-alkylguanine-DNA alkyltransferase (hAGT) into SNAP-tag, a self-labeling protein tag. Evolution of hAGT led not only to increased protein activity but also to higher stability, especially...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi2016537
更新日期：2012-02-07 00:00:00
abstract：:By use of site-directed mutagenesis, each prolyl residue in the lac permease of Escherichia coli at positions 28 (putative helix I), 31 (helix I), 61 (helix II), 89 (helix III), 97 (helix III), 123 (helix IV), 192 (putative hydrophilic region 7), 220 (helix VII), 280 (helix VIII), and 327 [helix X; Lolkema, J. S., et ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00219a019
更新日期：1991-02-05 00:00:00
abstract：:Rabbit muscle lactate dehydrogenase (LDH) was coupled to Sepharose in such a way that each molecule is expected to be attached via only one subunit. Dissociation of the bound active enzyme by several methods all yielded immobilized subunit derivatives which were inactive. These derivatives were capable of regenerating...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00664a013
更新日期：1976-09-21 00:00:00
abstract：:The sliding clamp of the Escherichia coli replisome is now understood to interact with many proteins involved in DNA synthesis and repair. A universal interaction motif is proposed to be one mechanism by which those proteins bind the E. coli sliding clamp, a homodimer of the beta subunit, at a single site on the dimer...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi036229j
更新日期：2004-05-18 00:00:00
abstract：:The two distinct domains of flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase, EC 1.1.2.3) are connected by a typical hinge peptide. To probe the importance of the structural integrity of the hinge region for efficient intraprotein electron transfer, three mutant enzymes have been constructed: H delta 3 [Sharp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi950457z
更新日期：1996-01-23 00:00:00
abstract：:Among the 118 genes upregulated by Pseudomonas aeruginosa in response to iron starvation [Ochsner, U. A., Wilderman, P. J., Vasil, A. I., and Vasil, M. L. (2002) Mol. Microbiol. 45, 1277-1287], we focused on the products of the two genes encoding electron transfer proteins, as a means of identifying the redox partners...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7013135
更新日期：2007-10-30 00:00:00
abstract：:The specific rate of superoxide (O2(•-)) production in the purified active crystallizable cytochrome b6f complex, normalized to the rate of electron transport, has been found to be more than an order of magnitude greater than that measured in isolated yeast respiratory bc1 complex. The biochemical and structural basis...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi4013534
更新日期：2013-12-17 00:00:00
abstract：:Proteins denature at low pH because of intramolecular electrostatic repulsions. The addition of salt partially overcomes this repulsion for some proteins, yielding a collapsed conformation called the A-state. A-states have characteristics expected for the molten globule, a notional kinetic protein folding intermediate...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981364v
更新日期：1998-12-01 00:00:00
abstract：:The repair of the endogenous lesion 8-oxo-7,8-dihydrodeoxyguanosine (8-oxodG) was investigated in the nucleotide excision repair mutant xeroderma pigmentosum D (XPD), using human normal or transformed XPD fibroblasts and the Chinese hamster XPD cell line UV5. In vivo repair of 8-oxodG induced by hydrogen peroxide trea...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi992610l
更新日期：2000-08-29 00:00:00
abstract：:A survey of RuvB protein-mediated ATP hydrolysis yields the following observations. (1) The RuvB protein exhibits a DNA-independent ATPase activity with a turnover number (based on a RuvB monomer) approaching 6 min-1 and a Km of 154 microM. Single-stranded DNA and linear duplex DNA have small but significant effects o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00030a019
更新日期：1995-08-01 00:00:00
abstract：:Retinoid X receptors (RXRs) are nuclear receptors that can activate transcription as homodimers or as obligate heterodimeric partners of other nuclear receptors. While the crystal structures of the RXR ligand-binding domains (LBD) have been previously determined, the dynamics of activation is less well characterized a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051474j
更新日期：2006-02-14 00:00:00
abstract：:The regulation of the rate-controlling enzyme in cholesterol biosynthesis and of the incorporation of [14C]oleate into cholesterol esters were studied in established lymphoid cell lines from normal subjects and compared with that of eight patients with genetic abnormalities of lipid metabolism. The activity of 3-hydro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00648a011
更新日期：1976-02-10 00:00:00
abstract：:The D/H fractionation factor (sigma) is the extent to which a hydrogen at a particular site becomes enriched in 2H over 1H relative to the solvent. A growing body of experimental evidence suggest that there is a correlation between the value of the fractionation factor and hydrogen-bond strength, with a lower sigma va...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00170a023
更新日期：1994-02-01 00:00:00
abstract：:The structural domains of human apolipoprotein(a) [apo(a)] and its interaction with apolipoprotein B-100 (apo B-100) in the lipoprotein(a) [Lp(a)] particle were investigated by limited proteolysis with thermolysin and cathepsin D. We characterized the proteolytic products by sodium dodecyl sulfate-polyacrylamide gradi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00177a026
更新日期：1994-03-22 00:00:00
abstract：:Thioredoxin reductase (TrxR) catalyzes the reduction of thioredoxin (Trx) by NADPH. A unique gene organization of TrxR and Trx has been found in Mycobacterium leprae, where TrxR and Trx are encoded by a single gene and, therefore, are expressed as a fusion protein (MlTrxR-Trx). This fusion enzyme is able to catalyze t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980754e
更新日期：1998-11-17 00:00:00
abstract：:A novel cationic lipid was separated from bovine brain white matter by a series of chromatographies on carboxymethyl-Sephadex and silica gel in chloroform and methanol. Its structure was identified unambiguously as de-N-acetyllactotriaosylceramide (deNAcLc(3)Cer) by mass spectrometry and (1)H NMR. The natural occurren...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0504411
更新日期：2005-07-12 00:00:00
abstract：:Insulin is very prone to form amyloid fibrils under slightly destabilizing conditions, and the B-chain region plays a critical role in the fibrillation. We show here that the isolated B-chain peptide of bovine insulin also forms fibrils at both acidic and neutral pH. When a mixture of insulin and the B-chain peptide w...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051658y
更新日期：2005-12-20 00:00:00
abstract：:The substrate specificities of two incorrectly annotated enzymes belonging to cog3964 from the amidohydrolase superfamily were determined. This group of enzymes are currently misannotated as either dihydroorotases or adenine deaminases. Atu3266 from Agrobacterium tumefaciens C58 and Oant2987 from Ochrobactrum anthropi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi301483z
更新日期：2013-01-08 00:00:00
abstract：:Glyoxylate carboligase (GCL) is a thiamin diphosphate (ThDP)-dependent enzyme, which catalyzes the decarboxylation of glyoxylate and ligation to a second molecule of glyoxylate to form tartronate semialdehyde (TSA). This enzyme is unique among ThDP enzymes in that it lacks a conserved glutamate near the N1' atom of Th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300893v
更新日期：2012-10-09 00:00:00
abstract：:Solvent proton nuclear magnetic dispersion studies at 25, 100, and 300 MHz have been performed on protocatechuate 3,4-dioxygenase (PCD) and its complexes with 3-chloro-4-hydroxybenzoate and 3-fluoro-4-hydroxybenzoate. Longitudinal and transverse relaxation rates were measured for these compounds and for the apoenzyme....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00312a024
更新日期：1984-08-14 00:00:00
abstract：:Integral membrane proteins, which include many cellular effector proteins and drug targets, can be difficult to produce, purify, and manipulate. Although the isolated ectodomains of many membrane proteins can be expressed as water soluble proteins, biological activity is frequently lost when these proteins are release...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi052600m
更新日期：2006-03-21 00:00:00
abstract：:Gram-negative bacteria, such as Escherichia coli, utilize efflux resistance systems in order to expel toxins from their cells. Heavy-metal resistance is mediated by resistance nodulation cell division (RND)-based efflux pumps composed of a tripartite complex that includes an RND-transporter, an outer-membrane factor (...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300596a
更新日期：2012-08-28 00:00:00
abstract：:The catalytic mechanism of recombinant soybean cytosolic ascorbate peroxidase (rsAPX) and a derivative of rsAPX in which a cysteine residue (Cys32) located close to the substrate (L-ascorbic acid) binding site has been modified to preclude binding of ascorbate [Mandelman, D., Jamal, J., and Poulos, T. L. (1998) Bioche...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0261591
更新日期：2002-11-19 00:00:00
abstract：:High-molecular mass thioredoxin reductases (TRs) are pyridine nucleotide disulfide oxidoreductases that catalyze the reduction of the disulfide bond of thioredoxin (Trx). Trx is responsible for reducing multiple protein disulfide targets in the cell. TRs utilize reduced β-nicotinamide adenine dinucleotide phosphate to...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi400931k
更新日期：2014-01-28 00:00:00
abstract：:Previously, we have demonstrated physical and functional interactions of the voltage-gated potassium channel Kv2.1 with the plasma membrane protein components of the exocytotic SNARE complex, syntaxin 1A, and the t-SNARE, syntaxin 1A/SNAP-25, complex. Importantly, the physical interaction of Kv2.1 with syntaxin was sh...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi800512p
更新日期：2008-08-12 00:00:00
abstract：:Post-translational phosphorylation and the related conformational changes in signaling proteins are responsible for regulating a wide range of subcellular processes. Human Pin1 is central to many of these cell signaling pathways in normal and aberrant subcellular processes, catalyzing cis-trans isomerization of the pe...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi2009954
更新日期：2011-11-08 00:00:00
abstract：:The seven serotypes (A-G) of botulinum neurotoxins (BoNTs) function through their proteolytic cleavage of one of three proteins (SNAP-25, Syntaxin, and VAMP) that form the SNARE complex required for synaptic vesicle fusion. The different BoNTs have very specific protease recognition requirements, between 15 and 50 ami...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi052518r
更新日期：2006-03-14 00:00:00
abstract：:The formation of ternary complexes involving serine proteases, borate, and an alcohol has important implications for understanding the physiological actions of borate and for the development of tight binding inhibitors for this class of enzymes. Recent studies of a related enzyme, gamma-glutamyl transpeptidase, which ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi025583z
更新日期：2002-05-14 00:00:00
