当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Directed evolution of a quorum-quenching lactonase from Mycobacterium avium subsp. paratuberculosis K-10 in the amidohydrolase superfamily.

Directed evolution of a quorum-quenching lactonase from Mycobacterium avium subsp. paratuberculosis K-10 in the amidohydrolase superfamily.

Abstract：

:The PLL(PTE-like lactonase)-group of enzymes within the amidohydrolase superfamily hydrolyze N-acyl-homoserine lactones (AHLs) that are involved in bacterial quorum-sensing pathways. These enzymes possess the (beta/alpha)(8)-barrel fold and serve as attractive templates for in vitro evolution and engineering of quorum-quenching biological molecules that can serve as antivirulence therapeutic agents. Using a quorum-quenching lactonase from Mycobacterium avium subsp. paratuberculosis K-10 (GI: 41409766) as the initial template for in vitro evolution experiments, we enhanced the catalytic efficiency and increased the substrate range of the wild-type enzyme through a single point mutation on the loop at the C-terminal end of the eighth beta-strand. This N266Y mutant had an increased value of k(cat)/K(M) of 30- and 32-fold toward 3-oxo-N-octanoyl-l-homoserine lactone and N-hexanoyl-l-homoserine lactone, respectively; the evolved mutant also exhibited lactonase activity toward 3-oxo-N-hexanoyl-l-homoserine lactone and N-butyryl-l-homoserine lactone, AHLs that were previously not hydrolyzed by the wild-type enzyme. This article reinforces the evolutionary potential of the (beta/alpha)(8)-barrel fold and highlights the possibility of using quorum-quenching lactonases in the amidohydrolase superfamily as templates for engineering biomolecules of therapeutic use.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Chow JY,Wu L,Yew WS

doi

10.1021/bi9004045

subject

Has Abstract

pub_date

2009-05-26 00:00:00

pages

4344-53

issue

20

eissn

0006-2960

issn

1520-4995

journal_volume

48

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Mutagenic Effects of a 2-Deoxyribonolactone-Thymine Glycol Tandem DNA Lesion in Human Cells.

    abstract：:Tandem DNA lesions containing two contiguously damaged nucleotides are commonly formed by ionizing radiation. Their effects on replication in mammalian cells are largely unknown. Replication of isolated 2-deoxyribonolactone (L), thymine glycol (Tg), and tandem lesion 5'-LTg was examined in human cells. Although nearly...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.9b01058

    authors： Naldiga S,Huang H,Greenberg MM,Basu AK

    更新日期：2020-02-04 00:00:00

  • Properties of Paracoccus denitrificans amicyanin.

    abstract：:Paracoccus denitrificans synthesizes an inducible, periplasmic, blue copper protein [Husain, M., & Davidson, V.L. (1985) J. Biol. Chem. 260, 14626-14629] that can be classified as an amicyanin on the basis of its ability to accept electrons from methylamine dehydrogenase. The amino acid composition and sequence of the...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00357a020

    authors： Husain M,Davidson VL,Smith AJ

    更新日期：1986-05-06 00:00:00

  • Conformational properties of nine purified cystathionine β-synthase mutants.

    abstract：:Protein misfolding due to missense mutations is a common pathogenic mechanism in cystathionine β-synthase (CBS) deficiency. In our previous studies, we successfully expressed, purified, and characterized nine CBS mutant enzymes containing the following patient mutations: P49L, P78R, A114V, R125Q, E176K, R266K, P422L, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi300435e

    authors： Hnízda A,Majtan T,Liu L,Pey AL,Carpenter JF,Kodíček M,Kožich V,Kraus JP

    更新日期：2012-06-12 00:00:00

  • Functional Evaluation of the π-Helix in the NAD(P)H:FMN Reductase of the Alkanesulfonate Monooxygenase System.

    abstract：:A subgroup of enzymes in the NAD(P)H:FMN reductase family is comprised of flavin reductases from two-component monooxygenase systems. The diverging structural feature in these FMN reductases is a π-helix centrally located at the tetramer interface that is generated by the insertion of an amino acid in a conserved α4 h...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b00544

    authors： Musila JM,L Forbes D,Ellis HR

    更新日期：2018-07-31 00:00:00

  • p21rac does not participate in the early interaction between p47-phox and cytochrome b558 that leads to phagocyte NADPH oxidase activation in vitro.

    abstract：:The phagocyte superoxide-generating NADPH oxidase, a multicomponent, membrane-bound electron transport chain, consists of cytochrome b558, p47-phox, p67-phox, and p21rac1 or p21rac2. The mechanisms of oxidase assembly are poorly understood. In previous studies using a cell-free NADPH oxidase system, we showed that pre...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00175a018

    authors： Kleinberg ME,Malech HL,Mital DA,Leto TL

    更新日期：1994-03-08 00:00:00

  • Cyclic peptides incorporating 4-carboxyphenylalanine and phosphotyrosine are potent inhibitors of pp60(c-)(src).

    abstract：:The protein tyrosine kinase, pp60(c-)(src), is involved in cellular signaling and is activated during mitosis and in various tumors. We have been employing cyclic decapeptides to identify the determinants for substrate binding and phosphorylation to develop inhibitors competitive with protein substrates of Src. A stru...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi992994f

    authors： Wang W,Ramdas L,Sun G,Ke S,Obeyesekere NU,Budde RJ,McMurray JS

    更新日期：2000-05-02 00:00:00

  • Chemical frustration in the protein folding landscape: grand canonical ensemble simulations of cytochrome c.

    abstract：:A grand canonical formalism is developed to combine discrete simulations for chemically distinct species in equilibrium. Each simulation is based on a perturbed funneled landscape. The formalism is illustrated using the alkaline-induced transitions of cytochrome c as observed by FTIR spectroscopy and with various othe...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi802293m

    authors： Weinkam P,Romesberg FE,Wolynes PG

    更新日期：2009-03-24 00:00:00

  • Effect of the inhibitor-resistant M69V substitution on the structures and populations of trans-enamine beta-lactamase intermediates.

    abstract：:The objective of this study was to determine the molecular factors that lead to beta-lactamase inhibitor resistance for the M69V variant in SHV-1 beta-lactamase. With mechanism-based inhibitors, the beta-lactamase forms an acyl-enzyme intermediate that consists of a trans-enamine derivative in the active site. This st...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi060990m

    authors： Totir MA,Padayatti PS,Helfand MS,Carey MP,Bonomo RA,Carey PR,van den Akker F

    更新日期：2006-10-03 00:00:00

  • Structural uncoupling between opposing domains of oxidized calmodulin underlies the enhanced binding affinity and inhibition of the plasma membrane Ca-ATPase.

    abstract：:Stabilization of the plasma membrane Ca-ATPase (PMCA) in an inactive conformation upon oxidation of multiple methionines in the calcium regulatory protein calmodulin (CaM) is part of an adaptive cellular response to minimize ATP utilization and the generation of reactive oxygen species (ROS) under conditions of oxidat...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0474113

    authors： Chen B,Mayer MU,Squier TC

    更新日期：2005-03-29 00:00:00

  • Distal regulation of heme binding of heme oxygenase-1 mediated by conformational fluctuations.

    abstract：:Heme oxygenase-1 (HO-1) is an enzyme that catalyzes the oxidative degradation of heme. Since free heme is toxic to cells, rapid degradation of heme is important for maintaining cellular health. There have been useful mechanistic studies of the HO reaction based on crystal structures; however, how HO-1 recognizes heme ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi5009694

    authors： Harada E,Sugishima M,Harada J,Fukuyama K,Sugase K

    更新日期：2015-01-20 00:00:00

  • Transmembrane topology of mammalian planar cell polarity protein Vangl1.

    abstract：:Vangl1 and Vangl2 are membrane proteins that play an important role in neurogenesis, and Vangl1/Vangl2 mutations cause neural tube defects in mice and humans. At the cellular level, Vangl proteins regulate the establishment of planar cell polarity (PCP), a process requiring membrane assembly of asymmetrically distribu...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi101767a

    authors： Iliescu A,Gravel M,Horth C,Apuzzo S,Gros P

    更新日期：2011-03-29 00:00:00

  • Local-access model for proton transfer in bacteriorhodopsin.

    abstract：:The accessibility of the retinal Schiff base in bacteriorhodopsin was studied in the D85N/D96N mutant where the proton acceptor and donor are absent. Protonation and deprotonation of the Schiff base after pH jump without illumination and in the photocycle of the unprotonated Schiff base were measured in the visible an...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9728396

    authors： Brown LS,Dioumaev AK,Needleman R,Lanyi JK

    更新日期：1998-03-17 00:00:00

  • Molecular characterization of limulin, a sialic acid binding lectin from the hemolymph of the horseshoe crab, Limulus polyphemus.

    abstract：:The sialic acid binding lectin, limulin, was isolated by gel filtration and ion-exchange chromatography from the hemolymph of Limulus polyphemus. When the purified protein was characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the presence of beta-mercaptoethanol, two major protein bands wer...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00638a026

    authors： Kaplan R,Li SS,Kehoe JM

    更新日期：1977-09-20 00:00:00

  • Escherichia coli class Ib ribonucleotide reductase contains a dimanganese(III)-tyrosyl radical cofactor in vivo.

    abstract：:Escherichia coli class Ib ribonucleotide reductase (RNR) converts nucleoside 5'-diphosphates to deoxynucleoside 5'-diphosphates in iron-limited and oxidative stress conditions. We have recently demonstrated in vitro that this RNR is active with both diferric-tyrosyl radical (Fe(III)(2)-Y(•)) and dimanganese(III)-Y(•) ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi101881d

    authors： Cotruvo JA,Stubbe J

    更新日期：2011-03-15 00:00:00

  • The N-terminus of apolipoprotein A-V adopts a helix bundle molecular architecture.

    abstract：:Previous studies of recombinant full-length human apolipoprotein A-V (apoA-V) provided evidence of the presence of two independently folded structural domains. Computer-assisted sequence analysis and limited proteolysis studies identified an N-terminal fragment as a candidate for one of the domains. C-Terminal truncat...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi800515c

    authors： Wong K,Beckstead JA,Lee D,Weers PM,Guigard E,Kay CM,Ryan RO

    更新日期：2008-08-19 00:00:00

  • Human liver alanine aminopeptidase. Inhibition by amino acids.

    abstract：:Human liver alanine aminopeptidase is inhibited by L-amino acids having hydrophobic side chains such as Phe, Tyr, Trp, Met, and Leu. Blocking of the amino group or the carboxyl group greatly reduces the inhibitory capacity of the amino acid. Kinetic studies demonstrate that inhibition of hydrolysis of the substrate L-...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00685a027

    authors： Garner CW,Behal FJ

    更新日期：1975-07-15 00:00:00

  • "Nonclassical" secretion of annexin A2 to the lumenal side of the enterocyte brush border membrane.

    abstract：:Annexin A2 is a member of the annexin family of Ca(2+)-dependent lipid binding proteins and believed to be engaged in membrane transport processes in a number of cell types. In small intestinal enterocytes, we localized annexin A2 to the brush border region, where it was found mainly on the lumenal side of the microvi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0355239

    authors： Danielsen EM,van Deurs B,Hansen GH

    更新日期：2003-12-16 00:00:00

  • Conformation of immunoglobulin M. 2. Nanosecond fluorescence depolarization analysis of segmental flexibility in anti-epsilon-l-dimethylamino-5-naphthalenesulfonyl-L-lysine anti-immunoglobulin from horse, pig, and shark.

    abstract：:The rotational motions of immunoglobulin M (IgM) were investigated by the nanosecond fluorescence depolarization technique. The fluorophore epsilon-1-dimethylamino-5-naphthalenesulfonyl-L-lysine (DNS-lysine) was specifically bound in the combining sites of anti-DNS IgM antibodies from the horse, pig, and nurse shark. ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00660a033

    authors： Holowka DA,Cathou RE

    更新日期：1976-07-27 00:00:00

  • Regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity and the esterification of cholesterol in human long term lymphoid cell lines.

    abstract：:The regulation of the rate-controlling enzyme in cholesterol biosynthesis and of the incorporation of [14C]oleate into cholesterol esters were studied in established lymphoid cell lines from normal subjects and compared with that of eight patients with genetic abnormalities of lipid metabolism. The activity of 3-hydro...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00648a011

    authors： Kayden HJ,Hatam L,Beratis NG

    更新日期：1976-02-10 00:00:00

  • Pump-1 cDNA codes for a protein with characteristics similar to those of classical collagenase family members.

    abstract：:Pump-1 cDNA has recently been isolated by screening a human tumor cDNA library with a transin (rat stromelysin) probe under low-stringency hybridization conditions. The cDNA codes for a potential protein with significant sequence similarity to the metalloproteinases collagenase and stromelysin, but which lacks the hem...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00439a004

    authors： Quantin B,Murphy G,Breathnach R

    更新日期：1989-06-27 00:00:00

  • Interaction of an aromatic dibromoisothiouronium derivative with the Ca(2+)-ATPase of skeletal muscle sarcoplasmic reticulum.

    abstract：:Isothiouronium compounds [Hoving, S., Bar-Shimon, M., Tijmes, J. J., Goldshleger, R., Tal, D. M., and Karlish, S. J. (1995) J. Biol. Chem. 270, 29788-29793] act as high-affinity competitive antagonists for Na(+) and K(+) (Rb(+)) on the renal Na(+)/K(+)-ATPase where they favor the E1 conformation. We have now character...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi026071n

    authors： Berman MC,Karlish SJ

    更新日期：2003-04-01 00:00:00

  • Differential interaction of lecithin-retinol acyltransferase with cellular retinol binding proteins.

    abstract：:Esterification of retinol (vitamin A alcohol) with long-chain fatty acids by lecithin-retinol acyltransferase (LRAT) is an important step in both the absorption and storage of vitamin A. Retinol in cells is bound by either cellular retinol binding protein (CRBP), present in most tissues including liver, or cellular re...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00144a014

    authors： Herr FM,Ong DE

    更新日期：1992-07-28 00:00:00

  • Destabilization of i-Motif DNA at Neutral pH by G-Quadruplex Ligands.

    abstract：:Numerous studies have been published stressing the importance of finding ligands that can bind specifically to DNA secondary structures. Several have identified ligands that are presented as having specific binding to the G-quadruplex; however, these were not originally tested on the complementary i-motif structure. T...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b00968

    authors： Abdelhamid MAS,Gates AJ,Waller ZAE

    更新日期：2019-01-29 00:00:00

  • Importance of residues Arg-167 and Gln-231 in both the allosteric and catalytic mechanisms of Escherichia coli aspartate transcarbamoylase.

    abstract：:Site-specific mutagenesis has been used to create two mutant versions of aspartate transcarbamoylase. Arg-167 and Gln-231, both previously identified as interacting with the portion of the bisubstrate analogue N-(phosphonoacetyl)-L-aspartate (PALA) that corresponds to aspartate [Krause, K. L., Voltz, K. W., & Lipscomb...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00468a003

    authors： Stebbins JW,Zhang Y,Kantrowitz ER

    更新日期：1990-04-24 00:00:00

  • A mechanistic investigation of the C-terminal redox motif of thioredoxin reductase from Plasmodium falciparum.

    abstract：:High-molecular mass thioredoxin reductases (TRs) are pyridine nucleotide disulfide oxidoreductases that catalyze the reduction of the disulfide bond of thioredoxin (Trx). Trx is responsible for reducing multiple protein disulfide targets in the cell. TRs utilize reduced β-nicotinamide adenine dinucleotide phosphate to...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi400931k

    authors： Snider GW,Dustin CM,Ruggles EL,Hondal RJ

    更新日期：2014-01-28 00:00:00

  • Characterization of recombinant Saccharomyces cerevisiae manganese-containing superoxide dismutase and its H30A and K170R mutants expressed in Escherichia coli.

    abstract：:All known Mn-containing superoxide dismutases (MnSODs) have a highly conserved histidine (His-30 in Escherichia coli FeSOD) in the active-site channel, and nearly all have an active-site arginine (Arg-170) that has been proposed to play a combined structural and functional role [Chan et al., Arch. Biochem. Biophys. 27...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi980167t

    authors： Borders CL Jr,Bjerrum MJ,Schirmer MA,Oliver SG

    更新日期：1998-08-11 00:00:00

  • Flash-induced relaxation changes of the EPR signals from the manganese cluster and YD reveal a light-adaptation process of photosystem II.

    abstract：:By exposing photosystem II (PSII) samples to an incrementing number of excitation flashes at room temperature, followed by freezing, we could compare the Mn-derived multiline EPR signal from the S2 oxidation state as prepared by 1, 5, 10, and 25 flashes of light. While the S2 multiline signals exhibited by these sampl...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi026848c

    authors： Peterson S,Ahrling KA,Högblom JE,Styring S

    更新日期：2003-03-11 00:00:00

  • Peptide binding by calmodulin and its proteolytic fragments and by troponin C.

    abstract：:Calmodulin and troponin C exhibit calcium-dependent binding of 1 mol/mol of dynorphin. The dissociation constants of the complexes, determined in 0.20 N KC1-1.0 mM CaCI2, pH 7.3, are 0.6 microM for calmodulin, 2.4 microM for rabbit fast skeletal muscle troponin C, and 9 microM for bovine heart troponin C. Experiments ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00306a016

    authors： Malencik DA,Anderson SR

    更新日期：1984-05-22 00:00:00

  • Solution structure of a DNA dodecamer containing the anti-neoplastic agent arabinosylcytosine: combined use of NMR, restrained molecular dynamics, and full relaxation matrix refinement.

    abstract：:The effect of araC incorporation into the dodecamer duplex [d(CGCGAATT) (araC)d(GCG)]2 was examined by comparing its nuclear magnetic resonance (NMR)-determined solution structure with that of the control duplex d[(CGCGAATTCGCG)]2. 1H and 31P resonances in both duplexes were assigned using a combination of 2-D 1H NMR ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：

    authors： Schweitzer BI,Mikita T,Kellogg GW,Gardner KH,Beardsley GP

    更新日期：1994-09-27 00:00:00

  • Conformational flexibility of luteinizing hormone-releasing hormone in aqueous solution. A carbon-13 spin-lattice relaxation time study.

    abstract：:The carbon-13 nuclear magnetic resonance (13C NMR) spectra of luteinizing hormone-releasing hormone (LH-RH) and lower homologous peptides have been assigned in aqueous solutions at various pH values. 13C spin-lattice relaxation times (T1) have been measured for all proton-bearing carbons at 25.2 and 67.9 MHz. From the...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00690a030

    authors： Deslauriers R,Levy GC,McGregor WH,Sarantakis K,Smith IC

    更新日期：1975-09-23 00:00:00