Abstract:
:The DNA interstrand cross-link (ICL) resulting from the C4'-oxidized abasic site (C4-AP) is a unique clustered lesion comprised of a cross-link adjacent to a nick. The ICL is a substrate for the UvrABC nucleotide excision repair system. The strand containing the nick is preferentially incised, but the nick influences the cleavage sites. Moreover, in approximately 15% of the molecules, the strand opposite the nick is incised, resulting in a more toxic double-strand break. This is the first example in which an interstrand cross-link is converted by nucleotide excision misrepair into a more deleterious double-strand break.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Sczepanski JT,Jacobs AC,Van Houten B,Greenberg MMdoi
10.1021/bi901006bsubject
Has Abstractpub_date
2009-08-18 00:00:00pages
7565-7issue
32eissn
0006-2960issn
1520-4995journal_volume
48pub_type
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