Deuteron nuclear magnetic resonance studies of phase equilibria in a lecithin-water system.

abstract：:The assignment of the 1H, 15N, 13CO, and 13C resonances of recombinant human interleukin-4 (IL-4), a protein of 133 residues and molecular mass of 15.4 kDa, is presented based on a series of 11 three-dimensional (3D) double- and triple-resonance heteronuclear NMR experiments. These studies employ uniformly labeled 15N...

journal_title：Biochemistry

authors： Powers R,Garrett DS,March CJ,Frieden EA,Gronenborn AM,Clore GM

更新日期：1992-05-05 00:00:00

abstract：:The peptide hormone bradykinin exerts important biological functions by binding to and activating bradykinin B2 receptors. B2 receptors belong to the seven transmembrane domain (7TM) receptor family. Cloning of the cDNA sequences for the rat, human, and mouse bradykinin B2 receptor revealed several in-frame AUG triple...

journal_title：Biochemistry

authors： AbdAlla S,Godovac-Zimmermann J,Braun A,Roscher AA,Müller-Esterl W,Quitterer U

更新日期：1996-06-11 00:00:00

abstract：:Pterin-free inducible nitric oxide synthase (iNOS) was reconstituted with tetrahydrobiopterin (H(4)B) or tetrahydrobiopterin analogues (5-methyl-H(4)B and 4-amino-H(4)B), and the ability of bound 5-methyl-H(4)B and 4-amino-H(4)B to support catalysis by either full-length iNOS (FLiNOS) or the isolated heme domain (HDiN...

journal_title：Biochemistry

authors： Hurshman AR,Krebs C,Edmondson DE,Marletta MA

更新日期：2003-11-18 00:00:00

abstract：:A series of peptide fragments of sperm whale myoglobin, corresponding to segments of the region between the G- and H-helices of the protein, have been synthesized and their conformational preferences investigated using circular dichroism and nuclear magnetic resonance spectroscopy in aqueous solution and in solvent mi...

journal_title：Biochemistry

authors： Shin HC,Merutka G,Waltho JP,Wright PE,Dyson HJ

更新日期：1993-06-29 00:00:00

abstract：:Escherichia coli non-heme-binding ferritin A (EcFtnA) is a spherical cagelike protein that is composed of 24 identical subunits. EcFtnA dissociates into 2-mers under acidic conditions and can reassemble into the native structure when the pH is increased. To understand how electrostatic interactions influence the assem...

journal_title：Biochemistry

authors： Sato D,Takebe S,Kurobe A,Ohtomo H,Fujiwara K,Ikeguchi M

更新日期：2016-01-26 00:00:00

abstract：:The binding of horse ferricytochrome c to yeast cytochrome c peroxidase at pH 6.0 in 8.7 mM phosphate buffer (0.0100 M ionic strength) is characterized by a small, unfavorable enthalpy change (+1.91 +/- 0.16 kcal mol-1) and a large, positive entropy change (+37 +/- 1 eu). The free energy of binding depends strongly up...

journal_title：Biochemistry

authors： Kresheck GC,Vitello LB,Erman JE

更新日期：1995-07-04 00:00:00

abstract：:It is now accepted that two or more conformations may exist within the same DNA molecule, thereby generating conformational junctions. The presence of B-Z junctions between right- and left-handed DNA conformations has been detected in plasmids by a number of techniques. Preliminary characterization of the first exampl...

journal_title：Biochemistry

authors： Sheardy RD,Winkle SA

更新日期：1989-01-24 00:00:00

abstract：:The occupancy of the central cavity of the membrane-embedded c ring of the ATP synthase of Escherichia coli was investigated with a photo-cross-linking approach. Single cysteine mutants were created at c subunit positions 4, 8, and 11, which are oriented to the inside of the ring. These cysteines were alkylated with r...

journal_title：Biochemistry

authors： Oberfeld B,Brunner J,Dimroth P

更新日期：2006-02-14 00:00:00

abstract：:Rabbit aortic smooth muscle cells were evaluated for their ability to synthesize and accumulate glycosaminoglycans (GAGs). Because of the sensitivity of the microtechniques utilized, it is possible to determine the specific radioactivity of the GAGs obtained after radioactive incorporation of [35S]SO4(2-) and [14C]glu...

journal_title：Biochemistry

authors： Namiki O,Faris B,Tschopp F,Füglistaller P,Hollander W,Franzblau C,Schmid K

更新日期：1980-04-29 00:00:00

abstract：:Human insulin-like growth factors I and II (hIGF-I, hIGF-II) are potent stimulators of cell and growth processes. They display high sequence similarity to both the A and B chains of insulin but contain an additional connecting C-domain, which reflects their secretion without specific packaging or precursor conversion....

journal_title：Biochemistry

authors： Brzozowski AM,Dodson EJ,Dodson GG,Murshudov GN,Verma C,Turkenburg JP,de Bree FM,Dauter Z

更新日期：2002-07-30 00:00:00

abstract：:Type IV collagen promotes integrin-mediated cell adhesion, spreading, and motility. Several regions within the triple-helical domain of type IV collagen have been identified as tumor cellular recognition sites. Among these regions, the alpha1(IV)531-543 sequence, designated L-Hep-III, promotes integrin-mediated tumor ...

journal_title：Biochemistry

authors： Li C,McCarthy JB,Furcht LT,Fields GB

更新日期：1997-12-09 00:00:00

abstract：:Base excision repair (BER) is the major corrective pathway for most spontaneous, oxidative, and alkylation DNA base and sugar damage. X-ray cross-complementing 1 (XRCC1) has been suggested to function at nearly every step of this repair process, primarily through direct protein-protein interactions. Using whole cell e...

journal_title：Biochemistry

authors： Wong HK,Kim D,Hogue BA,McNeill DR,Wilson DM 3rd

更新日期：2005-11-01 00:00:00

abstract：:Electron nuclear double resonance (ENDOR) of protons at Type 2 and Type 1 cupric active sites correlates with the enzymatic pH dependence, the mutation of nearby conserved, nonligating residues, and electron transfer in heterologously expressed Rhodobacter sphaeroides nitrite reductase. Wild-type enzyme showed a pH 6 ...

journal_title：Biochemistry

authors： Zhao Y,Lukoyanov DA,Toropov YV,Wu K,Shapleigh JP,Scholes CP

更新日期：2002-06-11 00:00:00

abstract：:Although the membrane-associated surfactant protein B (SP-B) is an essential component of lung surfactant, which is itself essential for life, the molecular basis for its activity is not understood. SP-B's biophysical functions can be partially mimicked by subfragments of the protein, including the C-terminus. We have...

journal_title：Biochemistry

authors： Booth V,Waring AJ,Walther FJ,Keough KM

更新日期：2004-12-07 00:00:00

abstract：:Amyloid nucleation through agitation was studied with beta2-microglobulin, which is responsible for dialysis-related amyloidosis, in the presence of salt under acid and neutral pH conditions. First, the aggregation of beta2-microglobulin in NaCl solutions was achieved by mildly agitating for 24 h at 37 degrees C prote...

journal_title：Biochemistry

authors： Sasahara K,Yagi H,Sakai M,Naiki H,Goto Y

更新日期：2008-02-26 00:00:00

abstract：:Resonances of the histidine region of human carbonic anhydrase B have been studied by proton magnetic resonance spectroscopy in the presence of seven sulfonamide inhibitors. Results of difference spectroscopy and observation of the C-2 resonance of an additional titratable histidine in some of these spectra suggest a ...

journal_title：Biochemistry

authors： Pesando JM,Grollman AP

更新日期：1975-02-25 00:00:00

abstract：:The aminoacylation kinetics of 19 different variants of yeast tRNATyr with nucleotide substitutions in positions 33-35 were determined. Substitution of the conserved uridine-33 does not alter the rate of aminoacylation. However, substitution of the anticodon position 34 or position 35 reduces Km from 2- to 10-fold and...

journal_title：Biochemistry

authors： Bare LA,Uhlenbeck OC

更新日期：1986-09-23 00:00:00

abstract：:The two-finger repeat in the human male-associated protein ZFY provides a model for comparative 2D-NMR studies of classical and variant Zn fingers. This repeat is defined in part by an alternation in spacing between consensus (HX3H) and variant (HX4H) histidine spacings. To investigate the effects of a "switch" betwee...

journal_title：Biochemistry

authors： Kochoyan M,Keutmann HT,Weiss MA

更新日期：1991-10-01 00:00:00

abstract：:Extensive molecular modeling with molecular dynamics simulations and van der Waals energy analyses were used to elucidate the striking finding that a mutagenic benzo[a]pyrene-derived DNA lesion, the base-displaced intercalated 10R-(+)-cis-anti-B[a]P-N(2)-dG (G*), manifests large differences in nucleotide excision repa...

journal_title：Biochemistry

authors： Mu H,Kropachev K,Chen Y,Zhang H,Cai Y,Geacintov NE,Broyde S

更新日期：2013-08-20 00:00:00

abstract：:Bacteriophage T7 codes for a single-stranded DNA binding protein. This protein is the product of gene 2.5 and has been found previously to stimulate specifically the activity of the phage-coded DNA polymerase. We report here that the T7 DNA binding protein also stimulates the activity of the phage-coded exonuclease. T...

journal_title：Biochemistry

authors： Roberts L,Sadowski P,Wong JT

更新日期：1982-11-09 00:00:00

abstract：:We examined the metabolism of exogenously added 13C-labeled fructose 1,6-bisphosphate (either labeled at the first and sixth carbons or labeled at the first carbon only) and of [2-13C]glucose in well-oxygenated and well-superfused hog carotid artery segments. Exogenously added fructose 1,6-bisphosphate was utilized by...

journal_title：Biochemistry

authors： Hardin CD,Roberts TM

更新日期：1995-01-31 00:00:00

abstract：:In addition to its superoxide dismutase (SOD) activity, Cu,Zn-superoxide dismutase (CuZnSOD) catalyzes the reductive decomposition of S-nitroso-L-glutathione (GSNO) in the presence of thiols such as L-glutathione (GSH). The GSNO-reductase activity but not the superoxide dismutase (SOD) activity of CuZnSOD is inhibited...

journal_title：Biochemistry

authors： Ye M,English AM

更新日期：2006-10-24 00:00:00

abstract：:The symmetric, tandem GU mismatch motifs, and , which only differ in the mismatch order, have an average difference in thermodynamic stability of 2 kcal/mol at 37 degrees C. Thermodynamic studies of duplexes containing these motifs indicate the effect is largely localized to the mismatches and adjacent base pairs. The...

journal_title：Biochemistry

authors： McDowell JA,He L,Chen X,Turner DH

更新日期：1997-07-01 00:00:00

abstract：:We have used a new fluorescent ATP analogue, 3'-(7-diethylaminocoumarin-3-carbonylamino)-3'-deoxyadenosine-5'-triphosphate (deac-aminoATP), to study the ATP hydrolysis mechanism of the single headed myosinV-S1. Our study demonstrates that deac-aminoATP is an excellent substrate for these studies. Although the deac-ami...

journal_title：Biochemistry

authors： Forgacs E,Cartwright S,Kovács M,Sakamoto T,Sellers JR,Corrie JE,Webb MR,White HD

更新日期：2006-10-31 00:00:00

abstract：:The beta- and delta-subunits of the nicotinic acetylcholine receptor from Torpedo californica were covalently photolabeled at the synaptic surface with the ATP photoaffinity analogue [alpha-32P]-8-azido-ATP. The specificity of labeling for nucleotide binding sites was demonstrated by the saturation of labeling with in...

journal_title：Biochemistry

authors： Carlson BJ,Raftery MA

更新日期：1993-07-27 00:00:00

abstract：:The first transmembrane (TM1) helix in the red cell anion exchanger (AE1, Band 3, or SLC4A1) acts as an internal signal anchor that binds the signal recognition particle and directs the nascent polypeptide chain to the endoplasmic reticulum (ER) membrane where it moves from the translocon laterally into the lipid bila...

journal_title：Biochemistry

authors： Fowler PW,Sansom MS,Reithmeier RA

更新日期：2017-02-07 00:00:00

abstract：:Class I major histocompatibility complex (MHC) proteins present peptide antigens to T cells during the immune response against viruses. Peptides are loaded into newly synthesized class I heterodimers in the endoplasmic reticulum such that most or all cell surface class I molecules contain peptides derived from endogen...

journal_title：Biochemistry

authors： Fahnestock ML,Johnson JL,Feldman RM,Tsomides TJ,Mayer J,Narhi LO,Bjorkman PJ

更新日期：1994-07-05 00:00:00

abstract：:Periodate-oxidized ATP (o-ATP) was prepared as an affinity label of nucleotide binding sites on the chloroform-released ox heart mitochondrial ATPase. In the presence of MgSO4, o-ATP is a substrate for the ATPase. It can act as a reversible, competitive inhibitor of ATPase activity and can also induce an irreversible ...

journal_title：Biochemistry

authors： Lowe PN,Beechey RB

更新日期：1982-08-17 00:00:00

abstract：:The physical origin of the extremely high thermal stability of tetraether liposomes composed of the polar lipid fraction E (PLFE) from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius has been investigated. The leakage rate of trapped 5, 6-carboxyfluorescein (5(6)CF) and the proton permeability in PLFE ...

journal_title：Biochemistry

authors： Komatsu H,Chong PL

更新日期：1998-01-06 00:00:00

abstract：:Two peptides designed for drug delivery were generated by the combination of a signal peptide with a nuclear localization sequence and are shown to facilitate the cellular internalization of small molecules which are covalently linked to these peptides. In order to understand the mechanism of internalization, the conf...

journal_title：Biochemistry

authors： Chaloin L,Vidal P,Heitz A,Van Mau N,Méry J,Divita G,Heitz F

更新日期：1997-09-16 00:00:00