Time-resolved fluorescence anisotropies of diphenylhexatriene and perylene in solvents and lipid bilayers obtained from multifrequency phase-modulation fluorometry.

abstract：:Two dioxygenases (ARD and ARD') were cloned from Klebsiella pneumoniae that catalyze different oxidative decomposition reactions of an advanced aci-reductone intermediate, CH(3)SCH(2)CH(2)COCH(OH)=CH(OH) (I), in the methionine salvage pathway. The two enzymes are remarkable in that they have the same polypeptide seque...

journal_title：Biochemistry

authors： Dai Y,Pochapsky TC,Abeles RH

更新日期：2001-05-29 00:00:00

abstract：:A procedure for universal 13C and/or 15N labeling of microbial peptides which are produced by fermentation in complex media and its application to two food-preserving bacteriocins from lactic acid bacteria are described. Isotopic enrichment of nisin A (from Lactococcus lactis) and of leucocin A (from Leuconostoc gelid...

journal_title：Biochemistry

authors： Sailer M,Helms GL,Henkel T,Niemczura WP,Stiles ME,Vederas JC

更新日期：1993-01-12 00:00:00

abstract：:Kinetics of reduction of phototrophic bacterial flavocytochromes c by exogenous flavin semiquinones and fully reduced flavins generated by laser flash photolysis have been studied. The mechanisms of reduction of Chromatium and Chlorobium flavocytochromes c are more similar to one another than previously thought. Neith...

journal_title：Biochemistry

authors： Cusanovich MA,Meyer TE,Tollin G

更新日期：1985-03-12 00:00:00

abstract：:Transcripts of Saccharomyces cerevisiae nuclear tRNA genes are normally terminated within a few nucleotides of the tRNA coding region, in contrast to mitochondrially encoded tRNAs, which are contained within polycistronic transcripts and thus require 3'-processing by mitochondrial endonucleases. We show that 3'-proces...

journal_title：Biochemistry

authors： Furter R,Snaith M,Gillespie DE,Hall BD

更新日期：1992-11-10 00:00:00

abstract：:The interactions of HIV-1 Env (gp120-gp41) with CD4 and coreceptors trigger a barrage of conformational changes in Env that drive the membrane fusion process. Various regions of gp41 have profound effects on HIV entry and budding. However, the precise interactions between gp41 and the membrane have not been elucidated...

journal_title：Biochemistry

authors： Viard M,Ablan SD,Zhou M,Veenstra TD,Freed EO,Raviv Y,Blumenthal R

更新日期：2008-02-19 00:00:00

abstract：:It is shown that the protochlorophyllide ester pool of etiolated higher plants is a faithful copy of the protochlorophyllide pool. It is made up of both monovinyl- and divinylprotochlorophyllide esters. Although the two tetrapyrroles exhibited similar emission maxima, they were distinguishable by their Soret excitatio...

journal_title：Biochemistry

authors： Belanger FC,Rebeiz CA

更新日期：1980-10-14 00:00:00

abstract：:Alpha-synuclein modulates dopamine homeostasis in dopamine-producing neurons of substantia nigra, partly through regulation of human dopamine transporter (hDAT) activity. To identify the underlying mechanisms, we disrupted the modulation of hDAT activity by wild-type (wt) alpha-synuclein, and its familial Parkinson's ...

journal_title：Biochemistry

authors： Wersinger C,Vernier P,Sidhu A

更新日期：2004-02-10 00:00:00

abstract：:Inducible dimers are powerful tools for controlling biological processes through colocalizing signaling molecules. To be effective, an inducible system should have a dissociation constant in the "off" state that is greater (i.e., weaker affinity) than the concentrations of the molecules that are being controlled, and ...

journal_title：Biochemistry

authors： Zimmerman SP,Hallett RA,Bourke AM,Bear JE,Kennedy MJ,Kuhlman B

更新日期：2016-09-20 00:00:00

abstract：:Escherichia coli MutS protein, which is required for mismatch repair, has a slow ATPase activity that obeys Michalelis-Menten kinetics. At 37 degrees C, the steady-state turnover rate for ATP hydrolysis is 1.0 +/- 0.3 min(-1) per monomer equivalent with a K(m) of 33 +/- 6 microM. Hydrolysis is competitively inhibited ...

journal_title：Biochemistry

authors： Bjornson KP,Allen DJ,Modrich P

更新日期：2000-03-21 00:00:00

abstract：:The positional specificity is the decisive enzyme property for classification of lipoxygenases and for the currently used lipoxygenase nomenclature. It has been reported before that soybean lipoxygenase-1, which oxygenates polyenoic fatty acids at alkaline pH to the corresponding n - 6 hydroperoxy derivative, exhibits...

journal_title：Biochemistry

authors： Ivanov I,Rathmann J,Myagkova G,Kuhn H

更新日期：2001-08-28 00:00:00

abstract：:Conformational changes in the prion protein cause transmissible spongiform encephalopathies, also referred to as prion diseases. In its native state, the prion protein is innocuous (PrPC), but it can misfold into a neurotoxic and infectious isoform (PrPSc). The full-length cellular form of the prion protein consists o...

journal_title：Biochemistry

authors： DeMarco ML,Daggett V

更新日期：2007-03-20 00:00:00

abstract：:The stacking of the bacteriochlorophyll (BChl) c macrocycles and the role of water in forming an aggregate sheet, in chlorosome, were examined by means of (13)C NMR spectroscopy, the measurement of the X-ray diffraction pattern, and (25)Mg NMR spectroscopy. (1) The stacking of the macrocycles, i.e., weakly overlapped ...

journal_title：Biochemistry

authors： Kakitani Y,Koyama Y,Shimoikeda Y,Nakai T,Utsumi H,Shimizu T,Nagae H

更新日期：2009-01-13 00:00:00

abstract：:Cerebrovascular deposition of fibrillar 39-42 amino acid amyloid beta-protein (Abeta), a condition known as cerebral amyloid angiopathy (CAA), is a key pathological feature of Alzheimer's disease and related disorders including hereditary cerebral hemorrhage with amyloidosis-Dutch type (HCHWA-D). Severe cases of CAA, ...

journal_title：Biochemistry

authors： Wagner MR,Keane DM,Melchor JP,Auspaker KR,Van Nostrand WE

更新日期：2000-06-27 00:00:00

abstract：:The spin-1/2-carrying NO molecule interacts with both the S1 and S2 states of the water oxidizing complex. The intermediates of the interaction can be resolved and trapped by NO treatment at subzero temperatures. At -30 degrees C and in the presence of approx. 500-700 microM NO, S1 loses the ability to yield by illumi...

journal_title：Biochemistry

authors： Goussias C,Ioannidis N,Petrouleas V

更新日期：1997-07-29 00:00:00

abstract：:We have investigated the occurrence of cyclic electron flow in intact spinach leaves. In particular, we have tested the hypothesis that cyclic flow requires the presence of supercomplexes in the thylakoid membrane or other strong associations between proteins. Using biochemical approaches, we found no evidence of the ...

journal_title：Biochemistry

authors： Breyton C,Nandha B,Johnson GN,Joliot P,Finazzi G

更新日期：2006-11-14 00:00:00

abstract：:Human transferrin (Tf) is responsible for the binding and transport of iron in the bloodstream of vertebrates. Delivery of this bound iron to cells occurs by a process of receptor-mediated endocytosis during which Tf releases its iron at the reduced endosomal pH of approximately 5.6. Iron release from Tf involves a la...

journal_title：Biochemistry

authors： Nurizzo D,Baker HM,He QY,MacGillivray RT,Mason AB,Woodworth RC,Baker EN

更新日期：2001-02-13 00:00:00

abstract：:The aa(3)-type quinol oxidase from Acidianus ambivalens is a divergent member of the heme-copper oxidases superfamily, namely, concerning the putative channels for intraprotein proton conduction. In this study, we used electrochemically induced FTIR difference spectroscopy to identify residues involved in redox-couple...

journal_title：Biochemistry

authors： Hellwig P,Gomes CM,Teixeira M

更新日期：2003-05-27 00:00:00

abstract：:The nonbulky 5',8-cyclopurine DNA lesions (cP) and the bulky, benzo[ a]pyrene diol epoxide-derived stereoisomeric cis- and trans- N2-guanine adducts (BPDE-dG) are good substrates of the human nucleotide excision repair (NER) mechanism. These DNA lesions were embedded at the In or Out rotational settings near the dyad ...

journal_title：Biochemistry

authors： Shafirovich V,Kolbanovskiy M,Kropachev K,Liu Z,Cai Y,Terzidis MA,Masi A,Chatgilialoglu C,Amin S,Dadali A,Broyde S,Geacintov NE

更新日期：2019-02-12 00:00:00

abstract：:We have investigated perturbations of the triplet-state properties of Trp residues in bacteriophage T4 lysozyme caused by point mutations using low-temperature phosphorescence and optical detection of triplet-state magnetic resonance (ODMR) spectroscopy. Five temperature-sensitive mutants have been studied in detail. ...

journal_title：Biochemistry

authors： Zang LH,Ghosh S,Maki AH

更新日期：1989-03-07 00:00:00

abstract：:We show that eight different serine proteinases--bovine chymotrypsins A and B, porcine pancreatic elastase I, proteinase K, Streptomyces griseus proteinases A and B, and subtilisins BPN' and Carlsberg--interact with turkey ovomucoid third domain at the same Leu18-Glu19 peptide bond, the reactive site of the inhibitor....

journal_title：Biochemistry

authors： Ardelt W,Laskowski M Jr

更新日期：1985-09-24 00:00:00

abstract：:Ferrochelatase is the terminal enzyme in the heme biosynthetic pathway. It catalyzes the insertion of ferrous iron into protoporphyrin IX to produce protoheme IX. The crystal structures of ferrochelatase from Saccharomyces cerevisiae in free form, in complex with Co(II), a substrate metal ion, and in complex with two ...

journal_title：Biochemistry

authors： Karlberg T,Lecerof D,Gora M,Silvegren G,Labbe-Bois R,Hansson M,Al-Karadaghi S

更新日期：2002-11-19 00:00:00

abstract：:The crystal structures of 7,8-dimethylisoalloxazine-10-acetic acid-tryptamine (1:1) tetrahydrate and 7,8-dimethylisoalloxazine-10-acetic acid-tyramine (1:1) tetrahydrate complexes were determined by the X-ray diffraction method, as models for flavin-tryptophan and flavin-tyrosine interactions in flavoproteins. The obs...

journal_title：Biochemistry

authors： Inoue M,Shibata M,Kondo Y,Ishida T

更新日期：1981-05-12 00:00:00

abstract：:The C2 domain is a Ca2+-dependent, membrane-targeting motif originally discovered in protein kinase C and recently identified in numerous eukaryotic signal-transducing proteins, including cytosolic phospholipase A2 (cPLA2) of the vertebrate inflammation pathway. Intracellular Ca2+ signals recruit the C2 domain of cPLA...

journal_title：Biochemistry

authors： Nalefski EA,Slazas MM,Falke JJ

更新日期：1997-10-07 00:00:00

abstract：:Multiply branched polylactosaminoglycans are expressed in glycoproteins and glycolipids of many cells. Interest in their biology stems from their abundant expression in early embryonal cells and from their ability to carry multiple lectin-binding determinants, which makes them prominent ligands and antagonists of cell...

journal_title：Biochemistry

authors： Leppanen A,Salminen H,Zhu Y,Maaheimo H,Helin J,Costello CE,Renkonen O

更新日期：1997-06-10 00:00:00

abstract：:The initiation of Escherichia coli chromosomal replication by DnaA protein is strongly influenced by the tight binding of the nucleotides ATP and ADP. Anionic phospholipids in a fluid bilayer promote the conversion of inactive ADP-DnaA protein to replicatively active ATP-DnaA protein in vitro, and thus likely play a k...

journal_title：Biochemistry

authors： Kitchen JL,Li Z,Crooke E

更新日期：1999-05-11 00:00:00

abstract：:The betagamma-crystallin superfamily consists of evolutionarily related proteins with domain topology similar to lens beta- and gamma-crystallins, formed from duplicated Greek key motifs. Ca(2+) binding was found in a few betagamma-crystallin members earlier, although its prevalence and diversity as inherent molecular...

journal_title：Biochemistry

authors： Aravind P,Mishra A,Suman SK,Jobby MK,Sankaranarayanan R,Sharma Y

更新日期：2009-12-29 00:00:00

abstract：:The Sir2 enzyme family is responsible for a newly classified chemical reaction, NAD(+)-dependent protein deacetylation. New peptide substrates, the reaction mechanism, and the products of the acetyl transfer to NAD(+) are described for SIR2. The final products of SIR2 reactions are the deacetylated peptide and the 2' ...

journal_title：Biochemistry

authors： Sauve AA,Celic I,Avalos J,Deng H,Boeke JD,Schramm VL

更新日期：2001-12-25 00:00:00

abstract：:A new fluorescent lipid analogue, bimanephosphatidylcholine, has been synthesized for use in lipid bilayers. This probe is well suited as an energy-transfer donor with N-(7-nitro-2,1,3-benzoxadiazol-4-yl)phosphatidylethanolamine as the acceptor. Dimyristoylphosphatidylethanolamine vesicles are prepared by sonication a...

journal_title：Biochemistry

authors： Pryor C,Bridge M,Loew LM

更新日期：1985-04-23 00:00:00

abstract：:In an attempt to understand the multifunctional involvement of beta(2)-glycoprotein I (beta(2)GPI) in autoimmune diseases, thrombosis, atherosclerosis, and inflammatory processes, substantial interest is focused on the interaction of beta(2)GPI with negatively charged ligands, in particular, with acidic phospholipids....

journal_title：Biochemistry

authors： Hammel M,Schwarzenbacher R,Gries A,Kostner GM,Laggner P,Prassl R

更新日期：2001-11-27 00:00:00

abstract：:The pre-steady-state reaction of indole and L-serine with the alpha 2 beta 2 complex of Escherichia coli tryptophan synthase has been investigated under different premixing conditions with rapid-scanning stopped-flow (RSSF) UV-visible spectroscopy for the spectral range 300-550 nm. When alpha 2 beta 2 was mixed with i...

journal_title：Biochemistry

authors： Drewe WF Jr,Dunn MF

更新日期：1986-05-06 00:00:00