N-glycosyl bond formation catalyzed by human alkyladenine DNA glycosylase.

abstract：:Light chain amyloidoses arise from the overproduction and abnormal deposition of the immunoglobulin light chain in various organs. LEN is the variable domain of an immunoglobulin light chain originally isolated from the urine of a patient suffering from multiple myeloma, with no sign of renal dysfunction or amyloidosi...

journal_title：Biochemistry

authors： Hu D,Qin Z,Xue B,Fink AL,Uversky VN

更新日期：2008-08-19 00:00:00

abstract：:The full three-dimensional structure of the catalytic domain of human stromelysin-1 (SCD) complexed to a novel and potent, nonpeptidic inhibitor has been determined by nuclear magnetic resonance spectroscopy (NMR). To accurately mimic assay conditions, the structure was obtained in Tris buffer at pH 6.8 and without th...

journal_title：Biochemistry

authors： Li YC,Zhang X,Melton R,Ganu V,Gonnella NC

更新日期：1998-10-06 00:00:00

abstract：:Nogo-66 receptor (NgR1) is a leucine-rich repeat (LRR) protein that forms part of a signaling complex modulating axon regeneration. Previous studies have shown that the entire LRR region of NgR1, including the C-terminal cap of the LRR, LRRCT, is needed for ligand binding, and that the adjacent C-terminal region (CT s...

journal_title：Biochemistry

authors： Wen D,Wildes CP,Silvian L,Walus L,Mi S,Lee DH,Meier W,Pepinsky RB

更新日期：2005-12-20 00:00:00

abstract：:The human antioxidant protein, HAH1, is an important participant in a Cu(I) transport chain, delivering one Cu(I) ion to the Wilson's (WND) or Menkes disease protein (MNK). Full geometry optimizations and second-derivative calculations were performed on several binding site models using the B3LYP functional to derive ...

journal_title：Biochemistry

authors： Op't Holt BT,Merz KM Jr

更新日期：2007-07-31 00:00:00

abstract：:The calcium pump [sarcoplasmic reticulum (SR) Ca(2+)-ATPase (SERCA)] transports Ca(2+) from the cytosol to the SR lumen at the expense of ATP hydrolysis and proton countertransport, thus playing a central role in Ca(2+) homeostasis and muscle contractility. Proton countertransport via deprotonation of transport site r...

journal_title：Biochemistry

authors： Ramírez-Salinas GL,Espinoza-Fonseca LM

更新日期：2015-08-25 00:00:00

abstract：:Escherichia coli, Clostridium sticklandii, and Methanococcus vannielii synthesize 75Se-labeled amino acid transfer ribonucleic acids [( 75Se]tRNAs) when grown with low levels (approximately equal to 1 microM) of 75SeO32-. When E. coli [75Se]tRNA was digested to nucleosides and analyzed by reversed-phase high-performan...

journal_title：Biochemistry

authors： Wittwer AJ,Tsai L,Ching WM,Stadtman TC

更新日期：1984-09-25 00:00:00

abstract：:The crystal structure of a cytochrome P450 from the thermoacidophile Picrophilus torridus, CYP231A2 (PTO1399), has been solved. This structure reveals a wide open substrate access channel. To better understand ligand-induced structural transitions in CYP231A2, protein-ligand interactions were investigated using 4-phen...

journal_title：Biochemistry

authors： Ho WW,Li H,Nishida CR,de Montellano PR,Poulos TL

更新日期：2008-02-19 00:00:00

abstract：:Information on the secondary structure and interactions of RNA is important to understand the biological function of RNA as well as in applying RNA as a tool for therapeutic purposes. Recently, the isoenergetic microarray mapping method was developed to improve the prediction of RNA secondary structure. Herein, for th...

journal_title：Biochemistry

authors： Fratczak A,Kierzek R,Kierzek E

更新日期：2011-09-06 00:00:00

abstract：:Tyramine β-monooxygenase (TβM), the insect homologue of dopamine β-monooxygenase, is a neuroregulatory enzyme that catalyzes the β-hydroxylation of tyramine to yield octopamine. Mutation of the methionine (Met) ligand to Cu(M) of TβM, Met471Cys, yielded a form of TβM that is catalytically active but susceptible to ina...

journal_title：Biochemistry

authors： Osborne RL,Zhu H,Iavarone AT,Hess CR,Klinman JP

更新日期：2012-09-25 00:00:00

abstract：:A recombinant system was developed for generation of steroid-receptor complexes in vitro. The DNA- and steroid-binding domains of the rat mineralocorticoid receptor were expressed in Escherichia coli as a fusion protein with glutathione S-transferase. The identity of the expressed recombinant protein was confirmed by ...

journal_title：Biochemistry

authors： Caamaño CA,Morano MI,Patel PD,Watson SJ,Akil H

更新日期：1993-08-24 00:00:00

abstract：:The X-ray crystal structures of Escherichia coli gamma-aminobutyrate aminotransferase unbound and bound to the inhibitor aminooxyacetate are reported. The enzyme crystallizes from ammonium sulfate solutions in the P3(2)21 space group with a tetramer in the asymmetric unit. Diffraction data were collected to 2.4 A reso...

journal_title：Biochemistry

authors： Liu W,Peterson PE,Carter RJ,Zhou X,Langston JA,Fisher AJ,Toney MD

更新日期：2004-08-31 00:00:00

abstract：:We have studied the structure of the CuB site in the binuclear heme-copper center of the fully oxidized form of the quinol-oxidizing cytochrome aa3-600 from Bacillus subtilis by EXAFS and ENDOR spectroscopy. This enzyme is member of the large superfamily of heme-copper respiratory oxidases, which catalyze the reductio...

journal_title：Biochemistry

authors： Fann YC,Ahmed I,Blackburn NJ,Boswell JS,Verkhovskaya ML,Hoffman BM,Wikström M

更新日期：1995-08-15 00:00:00

abstract：:Nitroalkane oxidase (NAO) from Fusarium oxysporum catalyzes the oxidation of neutral nitroalkanes to the corresponding aldehydes or ketones with the production of H(2)O(2) and nitrite. The flavoenzyme is a new member of the acyl-CoA dehydrogenase (ACAD) family, but it does not react with acyl-CoA substrates. We presen...

journal_title：Biochemistry

authors： Nagpal A,Valley MP,Fitzpatrick PF,Orville AM

更新日期：2006-01-31 00:00:00

abstract：:The rapid phosphorylation and dephosphorylation of a variety of proteins downstream of the endothelin receptors A and B was investigated following stimulation of human lung fibroblasts with endothelin-1. Changes in the phosphorylation of proteins involved in the cell cycle, cytoskeleton, membrane channels, transcripti...

journal_title：Biochemistry

authors： Stannard C,Soskic V,Godovac-Zimmermann J

更新日期：2003-12-02 00:00:00

abstract：:We investigated the dependence of certain properties of bile salt/lecithin mixed micelles on the molar ratio of the two components. UV absorption suggested that the micellar system is fundamentally altered on passing through a bile salt:lecithin molar ratio of 1.8:1. Differential scanning calorimetry confirmed this tr...

journal_title：Biochemistry

authors： Claffey WJ,Holzbach RT

更新日期：1981-01-20 00:00:00

abstract：:We have used site-directed mutagenesis to alter the [Fe-S] cluster composition of Escherichia coli dimethyl sulfoxide (DMSO) reductase (DmsABC). The electron-transfer subunit (DmsB) of this enzyme contains 16 Cys residues arranged in 4 groups (I-IV) which provide ligands to 4 [4Fe-4S] clusters [Cammack, R., & Weiner, ...

journal_title：Biochemistry

authors： Rothery RA,Weiner JH

更新日期：1991-08-27 00:00:00

abstract：:Complement components C5b-6 and C7 assemble to form C5b-7, which then interacts with membranes and commits the membrane attack complex to a target site. This protein-membrane association event was investigated to determine possible structural features that could contribute to a selective membrane attack. This system m...

journal_title：Biochemistry

authors： Silversmith RE,Nelsestuen GL

更新日期：1986-11-18 00:00:00

abstract：:An expression plasmid encoding the extracellular portion of the human tumor necrosis factor (TNF) type 1 receptor (TNF-R1) was constructed and used to generate a stable cell line secreting soluble TNF-R1 (sTNF-R1). The sTNF-R1 was purified, and its biochemical properties and its interactions with human TNF-alpha were ...

journal_title：Biochemistry

authors： Pennica D,Kohr WJ,Fendly BM,Shire SJ,Raab HE,Borchardt PE,Lewis M,Goeddel DV

更新日期：1992-02-04 00:00:00

abstract：:The three-dimensional structure of the fatty-acid-binding protein isolated from the flight muscle of the desert locust Schistocerca gregaria has been solved and refined to a crystallographic R-value of 18.5% for all measured X-ray data from 30.0- to 2.2-A resolution. Crystals employed in the investigation were grown f...

journal_title：Biochemistry

authors： Haunerland NH,Jacobson BL,Wesenberg G,Rayment I,Holden HM

更新日期：1994-10-18 00:00:00

abstract：:In mammalian cells, the incorporation of the 21st amino acid, selenocysteine, into proteins is guided by the Sec machinery. The function of this protein complex requires several protein-protein and protein-RNA interactions, leading to the incorporation of selenocysteine at UGA codons. It is guided by stem-loop structu...

journal_title：Biochemistry

authors： Oudouhou F,Casu B,Dopgwa Puemi AS,Sygusch J,Baron C

更新日期：2017-05-02 00:00:00

abstract：:We have isolated a new toxin, calitoxin (CLX), from the sea anemone Calliactis parasitica whose amino acid sequence differs greatly from that of other sea anemone toxins. The polypeptide chain contains 46 amino acid residues, with a molecular mass of 4886 Da and an isoelectric point at pH 5.4. The amino acid sequence ...

journal_title：Biochemistry

authors： Cariello L,de Santis A,Fiore F,Piccoli R,Spagnuolo A,Zanetti L,Parente A

更新日期：1989-03-21 00:00:00

abstract：:Listeriolysin O (LLO) is the most important virulence factor of the intracellular pathogen Listeria monocytogenes. Its main task is to enable escape of bacteria from the phagosomal vacuole into the cytoplasm. LLO belongs to the cholesterol-dependent cytolysin (CDC) family but differs from other members, as it exhibits...

journal_title：Biochemistry

authors： Bavdek A,Gekara NO,Priselac D,Gutiérrez Aguirre I,Darji A,Chakraborty T,Macek P,Lakey JH,Weiss S,Anderluh G

更新日期：2007-04-10 00:00:00

abstract：:A simple procedure is introduced for accurately defining the precision with which the Cartesian coordinates of any macromolecular structure are determined by nuclear Overhauser data. The method utilizes an ensemble of structures obtained from an array of independent simulated data sets derived from a final structure. ...

journal_title：Biochemistry

authors： Shriver J,Edmondson S

更新日期：1993-02-16 00:00:00

abstract：:During initiation of minus-strand synthesis by HIV-1 reverse transcriptase, a 3'-DNA-RNA-5' junction is formed involving the 3'-end of tRNAlys,3. The HIV-RT-associated RNase H cleaves the RNA template strand specifically, opposite the newly synthesized DNA strand. We have determined the crystal structure at 1.9 A reso...

journal_title：Biochemistry

authors： Mueller U,Maier G,Mochi Onori A,Cellai L,Heumann H,Heinemann U

更新日期：1998-09-01 00:00:00

abstract：:The effects of nonylphenol and 3,5-dibutyl-4-hydroxytoluene (BHT) on the activity of the (Ca2(+)-Mg2+)-ATPase of skeletal muscle sarcoplasmic reticulum have been studied. At high concentrations, both inhibit the ATPase activity of the ATPase either in native lipid or in bilayers of dioleoylphosphatidylcholine but, at ...

journal_title：Biochemistry

authors： Michelangeli F,Orlowski S,Champeil P,East JM,Lee AG

更新日期：1990-03-27 00:00:00

abstract：:Oxidative DNA damage can lead to cancer, and as enzymatic DNA repair systems become compromised during the aging process, the role of exogenous antioxidants becomes more critical. Here, we examined whether such non-enzymatic DNA repair can be effected by the common cellular antioxidant glutathione, investigating both ...

journal_title：Biochemistry

authors： Safaeipour M,Jauregui J,Castillo S,Bekarian M,Esparza D,Sanchez M,Stemp EDA

更新日期：2019-11-19 00:00:00

abstract：:4-Azido-2-nitrophenyl pyrophosphate (azido-PPi) labeled with 32P in the alpha position was prepared and used to photolabel beef heart mitochondrial F1. Azido-PPi was hydrolyzed by yeast inorganic pyrophosphatase, but not by mitochondrial F1-ATPase. Incubation of F1 with [alpha-32P]azido-PPi in the dark under condition...

journal_title：Biochemistry

authors： Michel L,Garin J,Issartel JP,Vignais PV

更新日期：1989-12-26 00:00:00

abstract：:RNA helicase DHX33 has been shown to participate in a variety of cellular activities, including ribosome biogenesis, protein translation, and gene transcription. We and others further discovered that DHX33 is strongly expressed in several types of human cancers and plays important roles in promoting cancer cell prolif...

journal_title：Biochemistry

authors： Wang X,Ge W,Zhang Y

更新日期：2019-01-29 00:00:00

abstract：:A hallmark of penicillin-binding protein 2 (PBP2) from penicillin-resistant strains of Neisseria gonorrhoeae is insertion of an aspartate after position 345. The insertion resides on a loop near the active site and is immediately adjacent to an existing aspartate (Asp346) that forms a functionally important hydrogen b...

journal_title：Biochemistry

authors： Fedarovich A,Cook E,Tomberg J,Nicholas RA,Davies C

更新日期：2014-12-09 00:00:00

abstract：:IFABP is a small beta-barrel protein with a short helix-turn-helix motif near the N-terminus that is thought to participate in the regulation of the uptake and delivery of fatty acids. In a previous work, we detected by near UV circular dichroism a reversible conformational transition of this protein occurring between...

journal_title：Biochemistry

authors： Arighi CN,Rossi JP,Delfino JM

更新日期：2003-06-24 00:00:00