Abstract：
:In mammalian cells, the incorporation of the 21st amino acid, selenocysteine, into proteins is guided by the Sec machinery. The function of this protein complex requires several protein-protein and protein-RNA interactions, leading to the incorporation of selenocysteine at UGA codons. It is guided by stem-loop structures localized in the 3' untranslated regions of the selenoprotein-encoding genes. Here, we conducted a global analysis of interactions between the Sec biosynthesis and incorporation components using a bioluminescence resonance energy transfer assay in mammalian cells that showed that selenocysteine synthase (SEPSECS), SECp43, and selenophosphate synthetases SEPHS1 and SEPHS2 form oligomers in eukaryotic cells. We also showed that SEPHS2 interacts with SEPSECS and SEPHS1; these interactions were confirmed by co-immunoprecipitation. To further analyze the interactions of SECp43, the protein was expressed in Escherichia coli, and small-angle X-ray scattering analysis revealed that it is a globular protein comprising two RNA-binding domains. Using phage display, we identified potential interaction sites and highlighted two residues (K166 and P167) required for its dimerization. The SECp43 structural model presented here constitutes the basis of future exploration of the protein-protein interactions among early components of the selenocysteine biosynthesis and incorporation pathway.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Oudouhou F,Casu B,Dopgwa Puemi AS,Sygusch J,Baron Cdoi
10.1021/acs.biochem.6b01116subject
Has Abstractpub_date
2017-05-02 00:00:00pages
2261-2270issue
17eissn
0006-2960issn
1520-4995journal_volume
56pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Factor VIII (fVIII) is synthesized as a single chain having a domainal sequence A1-A2-B-A3-C1-C2. Analysis of the proteolyic cleavage of fVIII by thrombin by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) identifies three fragments designated fVIIIA1, fVIIIA2, and fVIIIA3-C1-C2 with fragment(s) d...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00428a038
更新日期：1989-01-24 00:00:00
abstract：:Two antisera used in the radioimmunoassay for human fibrinopeptide A (FPA) which appear to have different immunochemical specificities have been tested for cross-reactivity with fibrinogen and with three fragments of fibrinogen which contain the FPA sequence. These fragments were the three-chain, NH2-terminal disulfid...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00651a004
更新日期：1976-03-23 00:00:00
abstract：:The "stalk" of the prokaryotic 50S ribosomal subunit is comprised of four copies of the protein L7/L12. In Escherichia coli, L7/L12 is a dimeric protein at micromolar concentrations, which is able to undergo rapid subunit exchange. A recent structural study indicated a tetrameric arrangement of the L12 proteins isolat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi048015n
更新日期：2005-03-08 00:00:00
abstract：:S-Protein/vitronectin is a serum glycoprotein that inhibits the lytic activity of the membrane attack complex of complement, i.e., of the complex including the proteins C5b, C6, C7, C8, and C9n. We show that intact S-protein/vitronectin or its cyanogen bromide generated fragments also inhibit the hemolysis mediated by...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00411a029
更新日期：1988-05-31 00:00:00
abstract：:Human cystathionine beta-synthase (CBS) is an essential enzyme for the removal of the toxic metabolite homocysteine. Heme and pyridoxal phosphate (PLP) cofactors are necessary to catalyze the condensation of homocysteine and serine to generate cystathionine. While the role for the PLP cofactor is thought to be similar...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0010874
更新日期：2001-01-16 00:00:00
abstract：:An S3 electron paramagnetic resonance (EPR) signal is observed in a variety of photosystem 2 (PS2) samples in which the oxygen-evolving complex (OEC) has been inhibited. These signals have been proposed to be due to an interaction, S2X+, between the manganese cluster in an oxidation state equivalent to S2 and an organ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00088a032
更新日期：1993-09-21 00:00:00
abstract：:Heme-derived linear tetrapyrroles (phytobilins) in phycobiliproteins and phytochromes perform critical light-harvesting and light-sensing roles in oxygenic photosynthetic organisms. A key enzyme in their biogenesis, phycocyanobilin:ferredoxin oxidoreductase (PcyA), catalyzes the overall four-electron reduction of bili...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi100728q
更新日期：2010-07-27 00:00:00
abstract：:Following ribonuclease digestion of methyl-3H-labeled B77 avian sarcoma virus RNA subunits, methylated oligonucleotides were isolated by diethylaminoethylcellulose chromotogrpahy. Partial nucleotide sequences were deduced from the known enzymatic specificities of the ribonucleases. In addition to methylated nucleoside...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00622a021
更新日期：1977-02-08 00:00:00
abstract：:The protein product of the Methanococcus jannaschii MJ0768 gene has been expressed in Escherichia coli, purified to homogeneity, and shown to catalyze the GTP-dependent addition of two l-glutamates to the l-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F(420)-0) to form F(420)-0-glutamyl-glutama...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034779b
更新日期：2003-08-19 00:00:00
abstract：:Deuterium-labeled dihydroorotates bearing one, two, or three deuteriums at the pair of C4 and C5 positions have been synthesized in high isotopic and chiral purity and characterized by NMR and mass spectroscopy. These substrates have been used with the FMN-containing biosynthetic dihydroorotate oxidase from Crithidia ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00307a033
更新日期：1984-06-05 00:00:00
abstract：:The carbon-13 (13C) nuclear magnetic resonance (NMR) behavior of the hyperactive analogue of luteinizing-hormone-releasing hormone (LH-RH), des-Gly-NH2(10)-[D-Leu6]LH-RH ethylamide, has been studied. The spectra are compared with those of the hypoactive des-Gly-NH2(10)-[L-Leu6]LH-RH ethylamide. Spin-lattice relaxation...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00666a020
更新日期：1976-10-19 00:00:00
abstract：:Fluorescent nucleobase analogues are used extensively to probe the structure and dynamics of nucleic acids. The fluorescence of the adenine analogue 2-aminopurine and the cytosine analogue pyrrolocytosine is significantly quenched when the bases are located in regions of double-stranded nucleic acids. To allow more de...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060479t
更新日期：2006-08-01 00:00:00
abstract：:The inducible nitric oxide synthase core oxygen domain (iNOS(COD)) is a homodimeric protein complex of ca. 100 kDa. In this work, the subunit disassembly and unfolding of the protein following a pH jump from 7.5 to 2.8 were monitored by on-line rapid mixing in conjunction with electrospray (ESI) time-of-flight mass sp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi047684y
更新日期：2005-02-22 00:00:00
abstract：:One way to improve the economic feasibility of biomass conversion is to enhance the catalytic efficiency of cellulases through protein engineering. This requires that high-resolution structures of cellulases be available. Here we present the structure of E2cd, the catalytic domain of the thermophilic endocellulase E2 ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00089a006
更新日期：1993-09-28 00:00:00
abstract：:Crystals of the Y195F mutant of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 were subjected to a double soaking procedure, in which they were first soaked in a solution containing the inhibitor acarbose and subsequently in a solution containing maltohexaose. The refined structure of the resultin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi952339h
更新日期：1996-04-02 00:00:00
abstract：:A full-length cDNA encoding a calcium-dependent protein kinase with a calmodulin-like domain from Arabidopsis thaliana (AK-1 for Arabidopsis kinase-1) has been expressed as a fusion protein (called AK-1-6H) in Escherichia coli and purified to near homogeneity with high specific activity (typically 2000 nmol min-1 mg-1...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00174a008
更新日期：1994-03-01 00:00:00
abstract：:β-Barrel membrane proteins are found in the outer membranes of mitochondria, chloroplasts, and Gram-negative bacteria; however, exactly how they are folded and inserted remains unknown. Over the past decade, both functional and structural studies have greatly contributed to addressing this elusive mechanism. It is kno...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/acs.biochem.5b00852
更新日期：2015-10-20 00:00:00
abstract：:The dynamic cytoskeletal network of microtubules and actin filaments can be disassembled by drugs. Cytoskeletal drugs work by perturbing the monomer-polymer equilibrium, thus changing the size and number of macromolecular crowders inside cells. Changes in both crowding and nonspecific surface interactions ("sticking")...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00299
更新日期：2020-07-21 00:00:00
abstract：:Snapshots of three consecutive steps in the proteolytic reaction of HIV-1 protease (PR) were obtained in crystal structures at resolutions of 1.2-1.4 Å. Structures of wild-type protease and two mutants (PR(V32I) and PR(I47V)) with V32I and I47V substitutions, which are common in drug resistance, reveal the gem-diol te...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi3008092
更新日期：2012-10-02 00:00:00
abstract：:(Z)-3-(Fluoromethyl)phosphoenolpyruvate has been synthesized in nine chemical steps from glyoxylic acid. The compound is stable at pH 3, but at pH 8 it decomposes within seconds to give 2-oxo-3-butenoate. When 3-(fluoromethyl)phosphoenolpyruvate is added to a solution of phosphoenolpyruvate carboxylase or pyruvate kin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00404a039
更新日期：1988-02-23 00:00:00
abstract：:The structure of HIV protease (HIV Pr) bound to JE-2147 (also named AG1776 or KNI-764) is determined here to 1.09 A resolution. This highest-resolution structure for HIV Pr allows refinement of anisotropic displacement parameters (ADPs) for all atoms. Clustering based on the directional information in ADPs defines two...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011781z
更新日期：2002-04-09 00:00:00
abstract：:Coiled coils (CCs) are among the best-understood protein folds. Nonetheless, there are gaps in our knowledge of CCs. Notably, CCs are likely to be structurally more dynamic than often considered. Here, we explore this in an abundant class of CCs, parallel dimers, focusing on polar asparagine (Asn) residues in the hydr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b00848
更新日期：2017-12-19 00:00:00
abstract：:The lateral and rotational dynamics of phosphoinositides and their interactions with proteins were characterized using pyrene-labeled lipid analogues. In these systems, the collision frequency of pyrene-labeled lipids was studied by monitoring the monomeric pyrene fluorescence yield as a function of their mole fractio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00028a022
更新日期：1995-07-18 00:00:00
abstract：:The direct transfer of NADH between A-B pairs of dehydrogenases and also the dissociation of NADH from individual E-NADH complexes have been investigated by transient stopped-flow kinetic techniques. Such A-B transfers of NADH occur without the intermediate dissociation of coenzyme into the aqueous solvent environment...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00379a006
更新日期：1987-03-10 00:00:00
abstract：:DNA alkylation-induced damage is one of the most efficacious anticancer therapeutic strategies. Enhanced DNA alkylation and weakened DNA repair capacity in cancer cells are responsible for the effectiveness of DNA-alkylating therapies. 5'-Flap endonuclease 1 (Fen1) is an important enzyme involved in base excision repa...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9010754
更新日期：2009-10-27 00:00:00
abstract：:The Rieske protein from Thermus thermophilus (TtRp) and a truncated version of the protein (truncTtRp), produced to achieve a low-pH crystallization condition, have been characterized using UV-visible and circular dichroism spectroscopies. TtRp and truncTtRp undergo a change in the UV-visible spectra with increasing p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901126u
更新日期：2009-10-20 00:00:00
abstract：:Annexin A1 is a multifunctional, calcium-dependent phospholipid binding protein involved in a host of processes including inflammation, regulation of neuroendocrine signaling, apoptosis, and membrane trafficking. Binding of annexin A1 to glycans has been implicated in cell attachment and modulation of annexin A1 funct...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi101121a
更新日期：2011-04-05 00:00:00
abstract：:Several common links between the structural chemistry of the chitolipooligosaccharides of Rhizobium and the general rhizobial membrane lipid and lipopolysaccharide chemistry of these bacteria have been uncovered. Aspects of common chemistry include sulfation, methylation, and the position and extent of fatty acyl chai...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00013a040
更新日期：1995-04-04 00:00:00
abstract：:A stereochemical analysis has been carried out on two vinylpyruvate hydratases (VPH), which convert 2-hydroxy-2,4-pentadienoate to 2-keto-4S-hydroxypentanoate in meta-fission pathways. Bacterial strains with this pathway can use aromatic compounds as sole sources of energy and carbon. The analysis was carried out usin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00552
更新日期：2016-07-26 00:00:00
abstract：:The vast majority of characterized oxygenases use bound cofactors to activate molecular oxygen to carry out oxidation chemistry. Here, we show that an enzyme of unknown activity, RhCC from Rhodococcus jostii RHA1, functions as an oxygenase, using 4-hydroxyphenylenolpyruvate as a substrate. This unique and complex reac...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi501200j
更新日期：2015-02-10 00:00:00
