Mechanistic studies with deuterated dihydroorotates on the dihydroorotate oxidase from Crithidia fasciculata.

abstract：:The C-terminus of interferon-gamma (IFNgamma) contains a nuclear localization sequence (NLS) required for the activation and nuclear translocation of the transcription factor STAT1alpha and induction of IFNgamma-activated genes. On the basis of this and other studies, we developed a peptide mimetic of IFNgamma that po...

journal_title：Biochemistry

authors： Subramaniam PS,Flowers LO,Haider SM,Johnson HM

更新日期：2004-05-11 00:00:00

abstract：:KLHL-12 is a substrate specific adapter protein for a Cul3-Ring ligase complex. It is a member of the Kelch β-propeller domain subclass of Cullin-Ring substrate recognition domains. This E3 ubiquitin ligase complex has many activities, including acting as a negative regulator of the Wnt signaling pathway by mediating ...

journal_title：Biochemistry

authors： Zhao B,Payne WG,Sai J,Lu Z,Olejniczak ET,Fesik SW

更新日期：2020-03-03 00:00:00

abstract：:Long-range intraprotein interactions give rise to many important protein behaviors. Understanding how energy is transduced through protein structures to either transmit a signal or elicit conformational changes is therefore a current challenge in structural biology. In an effort to understand such linkages, multiple V...

journal_title：Biochemistry

authors： Clarkson MW,Gilmore SA,Edgell MH,Lee AL

更新日期：2006-06-27 00:00:00

abstract：:The bacterial photosynthetic reaction center is the evolutionary ancestor of the Photosystem II reaction center. These proteins share the same fold and perform the same biological function. Nevertheless, the details of their molecular reaction mechanism differ. It is of significant biological and biochemical interest ...

journal_title：Biochemistry

authors： Krammer EM,Sebban P,Ullmann GM

更新日期：2009-02-17 00:00:00

abstract：:Time-resolved resonance Raman (TR(3)) and time-resolved step-scan (TRS(2)) FTIR spectroscopies have been used to probe the structural dynamics at the heme b(3) proximal and distal sites after carbon monoxide photolysis from fully reduced CO-bound nitric oxide reductase. The Raman spectra of the transient species exhib...

journal_title：Biochemistry

authors： Pinakoulaki E,Varotsis C

更新日期：2003-12-23 00:00:00

abstract：:Escherichia coli PurK, a dimeric N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) synthetase, catalyzes the conversion of 5-aminoimidazole ribonucleotide (AIR), ATP, and bicarbonate to N5-CAIR, ADP, and Pi. Crystallization of both a sulfate-liganded and the MgADP-liganded E. coli PurK has resulted in structures at 2....

journal_title：Biochemistry

authors： Thoden JB,Kappock TJ,Stubbe J,Holden HM

更新日期：1999-11-23 00:00:00

abstract：:One- and two-dimensional NMR spectroscopy and energy minimization calculations were used to investigate the conformation of a 2-aminofluorene- (AF-) modified model human c-H-ras1 protooncogene codon 61 deoxyoligonucleotide duplex, d(C1-A2-C3-C4-A5- [AF-G6]-G7-A8-A9-C10).-d(G11-T12-T13-C14-C15-T16 -G17-G18-T19-G20), in...

journal_title：Biochemistry

authors： Eckel LM,Krugh TR

更新日期：1994-11-22 00:00:00

abstract：:We previously showed that a bulky ring-strained cycloalkyne possessing a rhodamine fluorophore directly reacts (via strain-promoted click chemistry) with the azido group incorporated (via ligand-directed tosyl chemistry) into Asp160 in the 49 kDa subunit of complex I in bovine heart submitochondrial particles [Masuya,...

journal_title：Biochemistry

authors： Masuya T,Murai M,Ito T,Aburaya S,Aoki W,Miyoshi H

更新日期：2017-08-15 00:00:00

abstract：:The pH dependence of the 13C chemical shifts of the side-chain carboxyl carbons of all Asp and Glu residues in the reduced and oxidized states of human thioredoxin and in a mixed disulfide complex of human thioredoxin with a target peptide from the transcription factor NF kappa B has been investigated by multidimensio...

journal_title：Biochemistry

authors： Qin J,Clore GM,Gronenborn AM

更新日期：1996-01-09 00:00:00

abstract：:Production of single-strand breaks (ssb) and double-strand breaks (dsb) of PM2 phage DNA by several structurally related bleomycin (BLM) analogues was studied by gel electrophoresis. BLM A2 and BLM B2 produced a comparable extent of dsb. In various experiments, BLM A2 and BLM B2, at 22-41 ng/mL, degraded 50% of the fo...

journal_title：Biochemistry

authors： Huang CH,Mirabelli CK,Jan Y,Crooke ST

更新日期：1981-01-20 00:00:00

abstract：:The direct transfer of NADH between A-B pairs of dehydrogenases and also the dissociation of NADH from individual E-NADH complexes have been investigated by transient stopped-flow kinetic techniques. Such A-B transfers of NADH occur without the intermediate dissociation of coenzyme into the aqueous solvent environment...

journal_title：Biochemistry

authors： Srivastava DK,Bernhard SA

更新日期：1987-03-10 00:00:00

abstract：:Ca(2+)/calmodulin-dependent protein kinase kinases (CaMKKs) phosphorylate and activate specific downstream protein kinases, including CaMKI, CaMKIV, and 5'-AMP-activated protein kinase, which mediates a variety of Ca(2+) signaling cascades. CaMKKs have been shown to undergo autophosphorylation, although their role in ...

journal_title：Biochemistry

authors： Tokumitsu H,Hatano N,Fujimoto T,Yurimoto S,Kobayashi R

更新日期：2011-09-27 00:00:00

abstract：:By using targeted disulfide cross-linking, we have characterized structural changes that the Escherichia coli aspartate receptor undergoes upon modification of the four specific residues that are reversibly methylated during sensory adaptation. Cysteine residues were introduced at specific positions either in the cyto...

journal_title：Biochemistry

authors： Le Moual H,Quang T,Koshland DE Jr

更新日期：1998-10-20 00:00:00

abstract：:Hydrophobic interaction of 8-anilino-1-naphthalene sulfonic acid (ANS) with proteins is one of the widely used methods for characterizing/detecting partially folded states of proteins. We have carried out a systematic investigation on the effect of ANS, a charged hydrophobic fluorescent dye, on structural properties o...

journal_title：Biochemistry

authors： Ali V,Prakash K,Kulkarni S,Ahmad A,Madhusudan KP,Bhakuni V

更新日期：1999-10-12 00:00:00

abstract：:In addition to its superoxide dismutase (SOD) activity, Cu,Zn-superoxide dismutase (CuZnSOD) catalyzes the reductive decomposition of S-nitroso-L-glutathione (GSNO) in the presence of thiols such as L-glutathione (GSH). The GSNO-reductase activity but not the superoxide dismutase (SOD) activity of CuZnSOD is inhibited...

journal_title：Biochemistry

authors： Ye M,English AM

更新日期：2006-10-24 00:00:00

abstract：:The Vmax/Km (microM -1 min -1.) for bovine factor X activation by bovine factor IXa alpha, in the presence of sufficient [Ca2+] to saturate the initial reaction rate, was 0.007. When factor IXa beta was substituted for factor IXa alpha in this reaction, the Vmax/Km decreased to 0.001, suggesting that factor IXa alpha ...

journal_title：Biochemistry

authors： Link RP,Castellino FJ

更新日期：1983-08-16 00:00:00

abstract：:With knowledge of individual transfer free energies of chemical groups that become newly exposed on protein denaturation and assuming the group transfer free energy contributions are additive, it should be possible to predict the stability of a protein in the presence of denaturant. Unfortunately, several unresolved i...

journal_title：Biochemistry

authors： Auton M,Bolen DW

更新日期：2004-02-10 00:00:00

abstract：:Backbone amide dynamics of the Escherichia coli tryptophan repressor protein (WT-TrpR) and two functionally distinct variants, L75F-TrpR and A77V-TrpR, in their holo (l-tryptophan corepressor-bound) form have been characterized using (15)N nuclear magnetic resonance (NMR) relaxation. The three proteins possess very si...

journal_title：Biochemistry

authors： Tripet BP,Goel A,Copie V

更新日期：2011-06-14 00:00:00

abstract：:The signaling apparatus that controls bacterial chemotaxis is composed of a core complex containing chemoreceptors, the histidine autokinase CheA, and the coupling protein CheW. Site-specific spin labeling and pulsed dipolar ESR spectroscopy (PDS) have been applied to investigate the structure of a soluble ternary com...

journal_title：Biochemistry

authors： Bhatnagar J,Borbat PP,Pollard AM,Bilwes AM,Freed JH,Crane BR

更新日期：2010-05-11 00:00:00

abstract：:The interaction of the TAR RNA element of human immunodeficiency virus type 1 (HIV-1) with a 2'-O-methyl analogue of an RNA hairpin aptamer previously identified by in vitro selection [Ducongé, F., and Toulmé, J. J. (1999) RNA 5, 1605-1614] was characterized by UV-monitored thermal denaturation and surface plasmon res...

journal_title：Biochemistry

authors： Darfeuille F,Arzumanov A,Gait MJ,Di Primo C,Toulmé JJ

更新日期：2002-10-08 00:00:00

abstract：:Cytochrome P450 (P450) 3A4 is the most abundant human P450 and oxidizes a diversity of substrates, including various drugs, steroids, carcinogens, and macrolide natural products. In some reactions, positive cooperativity has been reported in microsomal studies. Flavonoids, e.g., 7,8-benzoflavone (alpha-naphthoflavone,...

journal_title：Biochemistry

authors： Ueng YF,Kuwabara T,Chun YJ,Guengerich FP

更新日期：1997-01-14 00:00:00

abstract：:Six affinity reagents containing chemically reactive groups, either on the phosphate residue at the 5'-end or on the 5'- or 3'-end internucleoside phosphate linkages of the oligothymidylate primers, were used to covalently modify the human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT). After covalent ...

journal_title：Biochemistry

authors： Andreola ML,Tarrago-Litvak L,Levina AS,Kolocheva TI,el Dirani-Diab R,Jamkovoy VI,Khalimskaya NL,Barr PJ,Litvak S,Nevinsky GA

更新日期：1993-04-13 00:00:00

abstract：:A newly reported strain of Vibrio cholerae, known as strain O139 Bengal, is the first instance of an encapsulated strain that has caused epidemic cholera. The O-antigenic capsule is the critical antigen for protective immunity. Since mapping of the antigenic epitopes will assist in the development of a protein conjuga...

journal_title：Biochemistry

authors： Gunawardena S,Fiore CR,Johnson JA,Bush CA

更新日期：1999-09-14 00:00:00

abstract：:Various omega-halogenated carboxy acids and amides were evaluated as potential active-site-directed reagents for alcohol dehydrogenase. 2-Bromoacetamide and bromoacetic and 3-bromopropionic acids inactivated the enzyme; AMP, NAD+, and NADH markedly decreased the rate of inactivation. Some omega-halogenated carboxyamid...

journal_title：Biochemistry

authors： Fries RW,Bohlken DP,Blakley RT,Plapp BV

更新日期：1975-11-18 00:00:00

abstract：:The p90 ribosomal S6 family of kinases (RSK) are potential drug targets, due to their involvement in cancer and other pathologies. There are currently only two known selective inhibitors of RSK, but the basis for selectivity is not known. One of these inhibitors is a naturally occurring kaempferol-α-L-diacetylrhamnosi...

journal_title：Biochemistry

authors： Utepbergenov D,Derewenda U,Olekhnovich N,Szukalska G,Banerjee B,Hilinski MK,Lannigan DA,Stukenberg PT,Derewenda ZS

更新日期：2012-08-21 00:00:00

abstract：:We investigated the effects of MA-5, a human-specific monoclonal antibody to the insulin receptor alpha-subunit, on transmembrane signaling in cell lines transfected with and expressing both normal human insulin receptors and receptors mutated in their beta-subunit tyrosine kinase domains. In cell lines expressing nor...

journal_title：Biochemistry

authors： Sung CK,Han XL,Brunetti A,Maddux B,Yamamoto-Honda R,Goldfine ID

更新日期：1992-01-14 00:00:00

abstract：:NADH:ubiquinone oxidoreductase (complex I) from bovine heart mitochondria is a highly complicated, energy transducing, membrane-bound enzyme. It contains 46 different subunits and nine redox cofactors: a noncovalently bound flavin mononucleotide and eight iron-sulfur clusters. The mechanism of complex I is not known. ...

journal_title：Biochemistry

authors： Sharpley MS,Shannon RJ,Draghi F,Hirst J

更新日期：2006-01-10 00:00:00

abstract：:The solution nuclear magnetic resonance (NMR) structures and backbone (15)N dynamics of the specialized acyl carrier protein (ACP), RpAcpXL, from Rhodopseudomonas palustris, in both the apo form and holo form modified by covalent attachment of 4'-phosphopantetheine at S37, are virtually identical, monomeric, and corre...

journal_title：Biochemistry

authors： Ramelot TA,Rossi P,Forouhar F,Lee HW,Yang Y,Ni S,Unser S,Lew S,Seetharaman J,Xiao R,Acton TB,Everett JK,Prestegard JH,Hunt JF,Montelione GT,Kennedy MA

更新日期：2012-09-18 00:00:00

abstract：:Retinoids function through conformational alterations of ligand-dependent nuclear transcription factors, the retinoic acid receptors, and retinoid X receptors. 9-cis-Retinoic acid is a known biological ligand for retinoid X receptors, but its synthesis pathway in vivo is largely unknown. Recently, we identified a cis-...

journal_title：Biochemistry

authors： Paik J,Vogel S,Piantedosi R,Sykes A,Blaner WS,Swisshelm K

更新日期：2000-07-11 00:00:00

abstract：:Two novel heme-containing peroxidases, one able to incorporate halogens into aromatic substrates and the other able to remove them, have recently been isolated from marine sources and initially characterized by Chen et al. [(1991) J. Biol. Chem. 266, 23909-23915; (1996) J. Biol. Chem. 271, 4609-4612]. The haloperoxida...

journal_title：Biochemistry

authors： Roach MP,Chen YP,Woodin SA,Lincoln DE,Lovell CR,Dawson JH

更新日期：1997-02-25 00:00:00