当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Crystal structures of unbound and aminooxyacetate-bound Escherichia coli gamma-aminobutyrate aminotransferase.

Crystal structures of unbound and aminooxyacetate-bound Escherichia coli gamma-aminobutyrate aminotransferase.

Abstract：

:The X-ray crystal structures of Escherichia coli gamma-aminobutyrate aminotransferase unbound and bound to the inhibitor aminooxyacetate are reported. The enzyme crystallizes from ammonium sulfate solutions in the P3(2)21 space group with a tetramer in the asymmetric unit. Diffraction data were collected to 2.4 A resolution for the unliganded enzyme and 1.9 A resolution for the aminooxyacetate complex. The overall structure of the enzyme is similar to those of other aminotransferase subgroup II enzymes. The ability of gamma-aminobutyrate aminotransferase to act on primary amine substrates (gamma-aminobutyrate) in the first half-reaction and alpha-amino acids in the second is proposed to be enabled by the presence of Glu211, whose side chain carboxylate alternates between interactions with Arg398 in the primary amine half-reaction and an alternative binding site in the alpha-amino acid half-reaction, in which Arg398 binds the substrate alpha-carboxylate. The specificity for a carboxylate group on the substrate side chain is due primarily to the presence of Arg141, but also requires substantial local main chain rearrangements relative to the structurally homologous enzyme dialkylglycine decarboxylase, which is specific for small alkyl side chains. No iron-sulfur cluster is found in the bacterial enzyme as was found in the pig enzyme [Storici, P., De Biase, D., Bossa, F., Bruno, S., Mozzarelli, A., Peneff, C., Silverman, R. B., and Schirmer, T. (2004) J. Biol. Chem. 279, 363-73.]. The binding of aminooxyacetate causes remarkably small changes in the active site structure, and no large domain movements are observed. Active site structure comparisons with pig gamma-aminobutyrate aminotransferase and dialkylglycine decarboxylase are discussed.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Liu W,Peterson PE,Carter RJ,Zhou X,Langston JA,Fisher AJ,Toney MD

doi

10.1021/bi049218e

subject

Has Abstract

pub_date

2004-08-31 00:00:00

pages

10896-905

issue

34

eissn

0006-2960

issn

1520-4995

journal_volume

43

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Thermodynamic measurements of the contributions of helix-connecting loops and of retinal to the stability of bacteriorhodopsin.

    abstract：:Thermodynamic studies of bacteriorhodopsin (BR) have been undertaken in order to investigate the factors that stabilize the structure of a membrane protein. The stability of the native, intact protein was compared to that of protein with retinal removed, and/or cleaved in one or two of the loops connecting the transme...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00152a020

    authors： Kahn TW,Sturtevant JM,Engelman DM

    更新日期：1992-09-22 00:00:00

  • Expression and Purification of ZASP Subdomains and Clinically Important Isoforms: High-Affinity Binding to G-Actin.

    abstract：:Z-disc-associated, alternatively spliced, PDZ motif-containing protein (ZASP) is a principal component of the sarcomere. The three prevalent isoforms of ZASP in skeletal muscle are generated by alternative splicing of exons 9 and 10. The long isoforms, either having (ZASP-L) or lacking exon 10 (ZASP-LΔex10), include a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.7b00067

    authors： Watts NR,Zhuang X,Kaufman JD,Palmer IW,Dearborn AD,Coscia S,Blech-Hermoni Y,Alfano C,Pastore A,Mankodi A,Wingfield PT

    更新日期：2017-04-11 00:00:00

  • Protoheme-carbon monoxide geminate kinetics.

    abstract：:Recombination kinetics of CO to protoheme after laser photolysis have been measured vs. temperature and viscosity. A 25-ns laser pulse was focused on the sample to produce an excitation rate of 10(9)/s per heme. This temporarily populates the heme-CO state of dissociated pairs which either separate or recombine on a p...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00358a008

    authors： Marden MC,Hazard ES 3rd,Gibson QH

    更新日期：1986-05-20 00:00:00

  • Post-translational phosphorylation of serine 74 of human deoxycytidine kinase favors the enzyme adopting the open conformation making it competent for nucleoside binding and release.

    abstract：:Deoxycytidine kinase (dCK) uses either ATP or UTP as a phosphoryl donor to catalyze the phosphorylation of nucleoside acceptors. The kinetic properties of human dCK are modulated in vivo by phosphorylation of serine 74. This residue is a part of the insert region and is distant from the active site. Replacing the seri...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi2001032

    authors： Hazra S,Szewczak A,Ort S,Konrad M,Lavie A

    更新日期：2011-04-12 00:00:00

  • Amino acid structures of multiple forms of amyloid-related serum protein SAA from a single individual.

    abstract：:Multiple forms of the acute-phase serum protein SAA were isolated from the lipoprotein fraction of plasma from a single individual. These protein forms were purified by size-exclusion, ion-exchange, and reverse-phase high-pressure liquid chromatography, and then the tryptic peptides were subjected to amino acid sequen...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00405a044

    authors： Dwulet FE,Wallace DK,Benson MD

    更新日期：1988-03-08 00:00:00

  • 31P NMR studies of ATP synthesis and hydrolysis kinetics in the intact myocardium.

    abstract：:The origin of the nuclear magnetic resonance (NMR)-measurable ATP in equilibrium Pi exchange and whether it can be used to determine net oxidative ATP synthesis rates in the intact myocardium were examined by detailed measurements of ATP in equilibrium Pi exchange rates in both directions as a function of the myocardi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00397a045

    authors： Kingsley-Hickman PB,Sako EY,Mohanakrishnan P,Robitaille PM,From AH,Foker JE,Uğurbil K

    更新日期：1987-11-17 00:00:00

  • Complexes of N antitermination protein of phage lambda with specific and nonspecific RNA target sites on the nascent transcript.

    abstract：:The mechanisms that control N protein dependent antitermination in phage lambda have counterparts in many eukaryotic systems, including specific regulatory interactions of the antitermination protein with the nascent RNA transcript. Here we describe the specific and nonspecific RNA binding modes of antitermination pro...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi961920q

    authors： Van Gilst MR,Rees WA,Das A,von Hippel PH

    更新日期：1997-02-11 00:00:00

  • Effect of surface curvature on stability, thermodynamic behavior, and osmotic activity of dipalmitoylphosphatidylcholine single lamellar vesicles.

    abstract：:The size and surface curvature dependence of the properties and stability of single lamellar vesicles have been investigated by using a variety of physicochemical techniques. Dipalmitoylphosphatidylcholine single lamellar vesicles of sizes ranging between 200 and 900 A in diameter have been prepared by the French pres...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00515a024

    authors： Lichtenberg D,Freire E,Schmidt CF,Barenholz Y,Felgner PL,Thompson TE

    更新日期：1981-06-09 00:00:00

  • The DNA sequence of the human beta-globin region is strongly biased in favor of long strings of contiguous purine or pyrimidine residues.

    abstract：:The DNA sequence of the human beta-globin region, comprising over 67 kilobase pairs, has been analyzed for the occurrence of strings of contiguous purine or pyrimidine residues. Tracts of 10 or more contiguous residues are found 4 times more frequently than would be expected with a random distribution of bases, so tha...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00398a050

    authors： Behe MJ

    更新日期：1987-12-01 00:00:00

  • Delocalizing trypsin specificity with metal activation.

    abstract：:Recognition for proteolysis by trypsin depends almost exclusively on tight binding of arginine or lysine side chains by the primary substrate specificity pocket. Although extended subsite interactions are important for catalysis, the majority of binding energy is localized in the P1 pocket. Analysis of the interaction...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9530191

    authors： Willett WS,Brinen LS,Fletterick RJ,Craik CS

    更新日期：1996-05-14 00:00:00

  • Photoinduced reactivity of the HIV-1 envelope glycoprotein with a membrane-embedded probe reveals insertion of portions of the HIV-1 Gp41 cytoplasmic tail into the viral membrane.

    abstract：:The interactions of HIV-1 Env (gp120-gp41) with CD4 and coreceptors trigger a barrage of conformational changes in Env that drive the membrane fusion process. Various regions of gp41 have profound effects on HIV entry and budding. However, the precise interactions between gp41 and the membrane have not been elucidated...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi701920f

    authors： Viard M,Ablan SD,Zhou M,Veenstra TD,Freed EO,Raviv Y,Blumenthal R

    更新日期：2008-02-19 00:00:00

  • Effect of phosphorylation on hydrogen-bonding interactions of the active site histidine of the phosphocarrier protein HPr of the phosphoenolpyruvate-dependent phosphotransferase system determined by 15N NMR spectroscopy.

    abstract：:The phosphocarrier protein HPr of the phosphoenolpyruvate-dependent sugar transport system of Escherichia coli can exist in a phosphorylated and a nonphosphorylated form. During phosphorylation, the phosphoryl group is carried on a histidine residue, His15. The hydrogen-bonding state of this histidine was examined wit...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00487a026

    authors： van Dijk AA,de Lange LC,Bachovchin WW,Robillard GT

    更新日期：1990-09-04 00:00:00

  • Spectroscopic studies of ascorbate oxidase. Electronic structure of the blue copper sites.

    abstract：:Low-temperature electronic absorption and room temperature circular dichroism (CD) and magnetic circular dichroism (MCD) spectra are reported for ascorbate oxidase. Bands attributable to d-d electronic transitions in the type 1 (blue) coppers (2B2 ground state) have been observed at 5800, 10000, and 12000 cm-1 (1725, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00510a044

    authors： Dooley DM,Dawson JH,Stephens PJ,Gray HB

    更新日期：1981-03-31 00:00:00

  • Heme-binding characteristics of the isolated PAS-A domain of mouse Per2, a transcriptional regulatory factor associated with circadian rhythms.

    abstract：:Neuronal PAS protein 2 (NPAS2), a heme-binding transcriptional regulatory factor, is involved in circadian rhythms. Period homologue (Per) is another important transcriptional regulatory factor that binds to cryptochrome (Cry). The resultant Per/Cry heterodimer interacts with the NPAS2/BMAL1 heterodimer to inhibit the...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi7023892

    authors： Kitanishi K,Igarashi J,Hayasaka K,Hikage N,Saiful I,Yamauchi S,Uchida T,Ishimori K,Shimizu T

    更新日期：2008-06-10 00:00:00

  • Linkage between ligand binding and control of tubulin conformation.

    abstract：:The effect of both antimitotic drugs and nucleotide analogues on the magnesium-induced self-association of purified tubulin into 42S double rings has been examined by sedimentation velocity. In the absence of magnesium, all complexes sedimented as the 5.8S species. The binding of colchicine to tubulin led to a small b...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00149a044

    authors： Shearwin KE,Timasheff SN

    更新日期：1992-09-01 00:00:00

  • Hydrogen-deuterium substitution and solvent effects on the nitrogen-15 nuclear magnetic resonance of gramicidin S: evaluation of secondary structure.

    abstract：:Complete assignments of nitrogen-15 resonances of gramicidin S have been made in dimethyl sulfoxide, trifluoroethanol, and in a solvent mixture of dimethyl sulfoxide (50%) and methanol (50%). The assignments are achieved by utilizing the secondary structure of gramicidin S, by comparing the nitrogen-15 spectrum of gra...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00606a005

    authors： Khaled MA,Urry DW,Sugano H,Miyoshi M,Izumiya N

    更新日期：1978-06-27 00:00:00

  • Structure-Related Roles for the Conservation of the HIV-1 Fusion Peptide Sequence Revealed by Nuclear Magnetic Resonance.

    abstract：:Despite extensive characterization of the human immunodeficiency virus type 1 (HIV-1) hydrophobic fusion peptide (FP), the structure-function relationships underlying its extraordinary degree of conservation remain poorly understood. Specifically, the fact that the tandem repeat of the FLGFLG tripeptide is absolutely ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.7b00745

    authors： Serrano S,Huarte N,Rujas E,Andreu D,Nieva JL,Jiménez MA

    更新日期：2017-10-17 00:00:00

  • Gene heterogeneity: a basis for alternative 5.8S rRNA processing.

    abstract：:Two bands of 5.8S rRNA were observed when the total RNA isolated from rat or mouse tissue was separated by electrophoresis on high-resolution polyacrylamide gels under denaturing conditions. The minor form, with a lower mobility, represented 15-35% of the total 5.8S rRNA, depending on the source of the tissue. Sequenc...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00311a011

    authors： Smith SD,Banerjee N,Sitz TO

    更新日期：1984-07-31 00:00:00

  • A general framework for development and data analysis of competitive high-throughput screens for small-molecule inhibitors of protein-protein interactions by fluorescence polarization.

    abstract：:Equilibrium binding experiments are widely used for the accurate characterization of binding and competitive binding behavior in biological systems. Modern high-throughput discovery efforts in chemical biology rely heavily upon this principle. Here, we derive exact analytical expressions for general competitive bindin...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi048233g

    authors： Roehrl MH,Wang JY,Wagner G

    更新日期：2004-12-28 00:00:00

  • Reconstruction of quaternary structures of class II tRNA synthetases by rational mutagenensis of a conserved domain.

    abstract：:Class II tRNA synthetases have long been known to have quaternary structures of alpha, alpha2, alpha2beta2, and alpha4, depending on the amino acid specificity and the organism from which the synthetase was isolated. Even the quaternary structures of enzymes for the same amino acid show variations in evolution. The ba...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi971788+

    authors： Ribas de Pouplana L,Schimmel P

    更新日期：1997-12-09 00:00:00

  • Interactions of anilinoacridines with nucleic acids: effects of substituent modifications on DNA-binding properties.

    abstract：:Spectroscopic methods are used to probe the interactions of several anilinoacridine analogues with calf thymus DNA over a wide range of temperatures and sodium chloride concentrations. The structurally similar compounds m-AMSA, AMSA (both active as antitumor agents), and o-AMSA (inactive as an antitumor agent) have be...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00231a025

    authors： Wadkins RM,Graves DE

    更新日期：1991-04-30 00:00:00

  • Cell-surface-associated tissue transglutaminase is a target of MMP-2 proteolysis.

    abstract：:MT1-MMP, a prototypic member of a membrane-type metalloproteinase subfamily, is an invasion promoting protease and an activator of MMP-2. In addition, MT1-MMP proteolysis regulates the functionality of cell-surface adhesion/signaling receptors including tissue transglutaminase (tTG). tTG is known to serve as an adhesi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi049266z

    authors： Belkin AM,Zemskov EA,Hang J,Akimov SS,Sikora S,Strongin AY

    更新日期：2004-09-21 00:00:00

  • Binding of cis- and trans-diamminedichloroplatinum(II) to deoxyribonucleic acid exposes nucleosides as measured immunochemically with anti-nucleoside antibodies.

    abstract：:We report the use of anti-nucleoside antibodies to probe for local denaturation of calf thymus DNA upon binding of the antitumor drug cis-diamminedichloroplatinum(II), cis-DDP, and the biologically inactive analogues trans-diamminedichloroplatinum(II), trans-DDP, and chloro(diethylenetriamine)platinum(II) chloride, [P...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00355a008

    authors： Sundquist WI,Lippard SJ,Stollar BD

    更新日期：1986-04-08 00:00:00

  • Mechanism of irreversible inhibition of O2 evolution in photosystem II by Tris(hydroxymethyl)aminomethane.

    abstract：:The dark reaction of tris(hydroxymethyl)aminomethane (Tris) with the O2-evolving center of photosystem II (PSII) in the S1 state causes irreversible inhibition of O2 evolution. Similar inhibition is observed for several other amines: NH3, CH3NH2, (CH3)2NH, ethanolamine, and 2-amino-2-ethyl-1,3-propanediol. In PSII mem...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00246a003

    authors： Rickert KW,Sears J,Beck WF,Brudvig GW

    更新日期：1991-08-13 00:00:00

  • Discontinuous release of heat at successive steps of oxygenation in human and bovine hemoglobin at pH 9.0.

    abstract：:We have measured the temperature dependence of the oxygen-binding isotherms of human and bovine hemoglobin at pH 9.0 in 0.1 M borate buffer. In both hemoglobins the ionization of the Bohr protons is finished at this pH; therefore, their heat does not interfere with the measurements. Two sets of curves have been obtain...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00227a006

    authors： Bucci E,Fronticelli C,Gryczynski Z

    更新日期：1991-04-02 00:00:00

  • Purification and characterization of the chaperonin 10 and chaperonin 60 proteins from Rhodobacter sphaeroides.

    abstract：:Two heat-shock proteins that show high identity with the Escherichia coli chaperonin 60 (groEL) and chaperonin 10 (groES) chaperonin proteins were purified and characterized from photolithoautotrophically grown Rhodobacter sphaeroides. The proteins were purified by using sucrose density gradient centrifugation and Mon...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00247a013

    authors： Terlesky KC,Tabita FR

    更新日期：1991-08-20 00:00:00

  • Conformational transition required for efficient splicing of transcripts from hybrid lambda promoter yeast tRNA gene fusion.

    abstract：:Fusion of a prokaryotic promoter to a yeast tRNA gene provides a means for uncoupling analyses of mutations affecting splicing from requirements for transcription and other processing steps. For this purpose, a phage lambda promoter was fused to the Saccharomyces cerevisiae tRNATyr(SUP3a) coding sequence. This fusion ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00240a017

    authors： Shapero MH,Greer CL

    更新日期：1991-07-02 00:00:00

  • Solution structure of the lipophosphoglycan of Leishmania donovani.

    abstract：:The three-dimensional solution structure of the repeating -PO4-6Gal beta 1-4Man alpha 1- disaccharide fragment of the lipophosphoglycan (LPG) derived from Leishmania donovani has been determined by use of a combination of homo- and heteronuclear NMR spin coupling constant measurements together with restrained molecula...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00118a004

    authors： Homans SW,Mehlert A,Turco SJ

    更新日期：1992-01-28 00:00:00

  • Roles of Ile209 and Ile210 on the heme pocket structure and regulation of histidine kinase activity of oxygen sensor FixL from Rhizobium meliloti.

    abstract：:FixL is a sensor histidine kinase having a heme-containing domain as an O(2) sensing site. In the study presented here, Ile209 and Ile210 located near the heme iron of the heme domain of Rhizobium meliloti FixL (RmFixL) were mutated, and the mutational effects on the regulation of the kinase activity and the heme pock...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi001184x

    authors： Mukai M,Nakamura K,Nakamura H,Iizuka T,Shiro Y

    更新日期：2000-11-14 00:00:00

  • Human immunodeficiency virus-1 protease. 1. Initial velocity studies and kinetic characterization of reaction intermediates by 18O isotope exchange.

    abstract：:The peptidolytic reaction of HIV-1 protease has been investigated by using four oligopeptide substrates, Ac-Ser-Gln-Asn-Tyr-Pro-Val-Val-NH2, Ac-Arg-Ala-Ser-Gln-Asn-Tyr-Pro-Val-Val-NH2, Ac-Ser-Gln-Ser-Tyr-Pro-Val-Val-NH2, and Ac-Arg-Lys-Ile-Leu-Phe-Leu-Asp-Gly-NH2, that resemble two cleavage sites found within the natu...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00098a023

    authors： Hyland LJ,Tomaszek TA Jr,Roberts GD,Carr SA,Magaard VW,Bryan HL,Fakhoury SA,Moore ML,Minnich MD,Culp JS

    更新日期：1991-08-27 00:00:00