Abstract:
:Complete assignments of nitrogen-15 resonances of gramicidin S have been made in dimethyl sulfoxide, trifluoroethanol, and in a solvent mixture of dimethyl sulfoxide (50%) and methanol (50%). The assignments are achieved by utilizing the secondary structure of gramicidin S, by comparing the nitrogen-15 spectrum of gramicidin S with that of di-N-methylphenylalanine-gramicidin S and by taking into account the distinguishable value of nitrogen-15 chemical shift for valine in model compounds. Deuterium substitution for labile peptide protons was performed to delineate solvent shielded and deshielded peptide nitrogens and to substantiate further the signal assignments. The solvent titration on going from dimethyl sulfoxide to trifluoroethanol was also performed and shown to have a large deshielding effect on the peptide nitrogen whose corresponding peptide carbonyl, within the peptide moiety, was accessible to the trifluroethanol solvent.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Khaled MA,Urry DW,Sugano H,Miyoshi M,Izumiya Ndoi
10.1021/bi00606a005subject
Has Abstractpub_date
1978-06-27 00:00:00pages
2490-4issue
13eissn
0006-2960issn
1520-4995journal_volume
17pub_type
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