Cold denaturation of the molten globule states of apomyoglobin and a profile for protein folding.


:Protein folding is a process in which an extended polypeptide chain acquires compact packing through the formation of specific secondary and tertiary structures and hydrophobic interactions. Although much attention has been paid to secondary and tertiary structures, there is no definitive view about the relationship between these structures, compactness, and hydrophobic interactions during the process of protein folding. We show here that the molten globule intermediates of horse apomyoglobin exhibit cold denaturation in addition to heat denaturation, which indicates that the heat capacity change upon unfolding is positive and significant. This demonstrates a small but distinct contribution of hydrophobic interactions to the stability of the molten globule state. We determined the radius of gyration of the various conformational states of horse apomyoglobin and holomyoglobin by measuring small angle X-ray scattering. By comparing the conformational states in terms of secondary structure, radius of gyration, and change in heat capacity upon unfolding, we constructed a folding profile. The profile shows that the protein becomes more compact with formation of the secondary structure, but does not form substantial hydrophobic interactions until a later rate-limiting stage when tight packing of the protein side chains occurs. A very similar profile was also obtained with horse cytochrome c. We propose that the folding profile obtained with these proteins will be common to many globular proteins.






Nishii I,Kataoka M,Tokunaga F,Goto Y




Has Abstract


1994-04-26 00:00:00












  • Selenium as an electron acceptor during the catalytic mechanism of thioredoxin reductase.

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    pub_type: 杂志文章


    authors: Lothrop AP,Snider GW,Ruggles EL,Patel AS,Lees WJ,Hondal RJ

    更新日期:2014-02-04 00:00:00

  • Conformational and aggregational properties of the gene 9 minor coat protein of bacteriophage M13 in membrane-mimicking systems.

    abstract::The membrane-bound state of the gene 9 minor coat protein of bacteriophage M13 was studied in various membrane-mimicking systems, including organic solvents, detergent micelles, and phospholipid bilayers. For this purpose we determined the conformational and aggregational properties of the chemically synthesized prote...


    pub_type: 杂志文章


    authors: Houbiers MC,Spruijt RB,Wolfs CJ,Hemminga MA

    更新日期:1999-01-19 00:00:00

  • Role of the arginine-45 salt bridge in ligand dissociation from sperm whale carboxymyoglobin as probed by photoacoustic calorimetry.

    abstract::The dynamics of the enthalpy and volume changes found in the photodissociation of CO from sperm whale carboxymyoglobin and two site-directed mutants in which arginine-45 is replaced by glycine and asparagine are examined by photoacoustic calorimetry. An intermediate is observed whose lifetime at 20 degrees C is 700 ns...


    pub_type: 杂志文章


    authors: Westrick JA,Peters KS,Ropp JD,Sligar SG

    更新日期:1990-07-17 00:00:00

  • Evidence for a methylammonium-binding site on methylamine dehydrogenase of Thiobacillus versutus.

    abstract::The nonconvertible substrate analogues di-, tri-, and tetramethylammonium are bound with fairly high affinity to oxidized methylamine dehydrogenase (MADHox) from Thiobacillus versutus and induce the same red-shift in the optical absorbance spectrum of MADHox as do the monovalent cations Cs+, Rb+, and NH4+. Like the mo...


    pub_type: 杂志文章


    authors: Gorren AC,Moenne-Loccoz P,Backes G,de Vries S,Sanders-Loehr J,Duine JA

    更新日期:1995-10-10 00:00:00

  • Structure of subtilosin A, a cyclic antimicrobial peptide from Bacillus subtilis with unusual sulfur to alpha-carbon cross-links: formation and reduction of alpha-thio-alpha-amino acid derivatives.

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    authors: Kawulka KE,Sprules T,Diaper CM,Whittal RM,McKay RT,Mercier P,Zuber P,Vederas JC

    更新日期:2004-03-30 00:00:00

  • Intravesicular calcium transient during calcium release from sarcoplasmic reticulum.

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    pub_type: 杂志文章


    authors: Ikemoto N,Antoniu B,Kang JJ,Mészáros LG,Ronjat M

    更新日期:1991-05-28 00:00:00

  • Photolabile protecting groups for an acetylcholine receptor ligand. Synthesis and photochemistry of a new class of o-nitrobenzyl derivatives and their effects on receptor function.

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    pub_type: 杂志文章


    authors: Walker JW,McCray JA,Hess GP

    更新日期:1986-04-08 00:00:00

  • A new inhibitor of coupled oxidative phosphorylation, 5-hydroxynaphthalenedicarboxylic anhydride, a derivative of a carcinogenic polynuclear hydrocarbon.

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    pub_type: 杂志文章


    authors: Hadler HI,Demetriou JM

    更新日期:1975-12-02 00:00:00

  • Rapid Oxidation Following Photoreduction in the Avian Cryptochrome4 Photocycle.

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    pub_type: 杂志文章


    authors: Otsuka H,Mitsui H,Miura K,Okano K,Imamoto Y,Okano T

    更新日期:2020-09-29 00:00:00

  • Dynamic inhomogeneity in the photodynamics of cyanobacterial phytochrome Cph1.

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    pub_type: 杂志文章


    authors: Kim PW,Rockwell NC,Martin SS,Lagarias JC,Larsen DS

    更新日期:2014-05-06 00:00:00

  • Energetics of internal GU mismatches in ribooligonucleotide helixes.

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    pub_type: 杂志文章


    authors: Sugimoto N,Kierzek R,Freier SM,Turner DH

    更新日期:1986-09-23 00:00:00

  • Efficient delivery of long-chain fatty aldehydes from the Nostoc punctiforme acyl-acyl carrier protein reductase to its cognate aldehyde-deformylating oxygenase.

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    pub_type: 杂志文章


    authors: Warui DM,Pandelia ME,Rajakovich LJ,Krebs C,Bollinger JM Jr,Booker SJ

    更新日期:2015-02-03 00:00:00

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    pub_type: 杂志文章


    authors: Fredenburg RA,Rospigliosi C,Meray RK,Kessler JC,Lashuel HA,Eliezer D,Lansbury PT Jr

    更新日期:2007-06-19 00:00:00

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    pub_type: 杂志文章


    authors: Garza-Ramos G,Tuena de Gomez-Puyou M,Gomez-Puyou A,Yüksel KU,Gracy RW

    更新日期:1994-06-07 00:00:00

  • Thermodynamic studies of the core histones: stability of the octamer subunits is not altered by removal of their terminal domains.

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    pub_type: 杂志文章


    authors: Karantza V,Freire E,Moudrianakis EN

    更新日期:2001-10-30 00:00:00

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    pub_type: 杂志文章


    authors: Schwehm JM,Kristyanne ES,Biggers CC,Stites WE

    更新日期:1998-05-12 00:00:00

  • Replacement of disulfides by amide bonds in the relaxin-like factor (RLF/INSL3) reveals a role for the A11-B10 link in transmembrane signaling.

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    authors: Büllesbach EE,Schwabe C

    更新日期:2012-05-22 00:00:00

  • Distal site aspartate is essential in the catalase activity of catalase-peroxidases.

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    pub_type: 杂志文章


    authors: Jakopitsch C,Auer M,Regelsberger G,Jantschko W,Furtmüller PG,Rüker F,Obinger C

    更新日期:2003-05-13 00:00:00

  • Phosphorylation and functions of inhibitor-2 family of proteins.

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    pub_type: 杂志文章


    authors: Li M,Satinover DL,Brautigan DL

    更新日期:2007-03-06 00:00:00

  • CofE catalyzes the addition of two glutamates to F420-0 in F420 coenzyme biosynthesis in Methanococcus jannaschii.

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    pub_type: 杂志文章


    authors: Li H,Graupner M,Xu H,White RH

    更新日期:2003-08-19 00:00:00

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    authors: Hadfield A,Shammas C,Kryger G,Ringe D,Petsko GA,Ouyang J,Viola RE

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    pub_type: 杂志文章


    authors: Sikkema KD,O'Leary MH

    更新日期:1988-02-23 00:00:00

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    pub_type: 杂志文章


    authors: Nath A,Atkins WM

    更新日期:2008-01-08 00:00:00

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    pub_type: 杂志文章


    authors: Jaravine VA,Nolde DE,Reibarkh MJ,Korolkova YV,Kozlov SA,Pluzhnikov KA,Grishin EV,Arseniev AS

    更新日期:1997-02-11 00:00:00

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    pub_type: 杂志文章


    authors: Wang H,Vath GM,Gleason KJ,Hanna PE,Wagner CR

    更新日期:2004-06-29 00:00:00

  • Biosynthesis of GDP-d-glycero-α-d-manno-heptose for the Capsular Polysaccharide of Campylobacter jejuni.

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    pub_type: 杂志文章


    authors: Huddleston JP,Raushel FM

    更新日期:2019-09-17 00:00:00

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    pub_type: 杂志文章,评审


    authors: Feng Z,Zeng M,Chen X,Zhang M

    更新日期:2018-05-01 00:00:00

  • Tubulin polymerization promoting proteins (TPPPs): members of a new family with distinct structures and functions.

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    pub_type: 杂志文章


    authors: Vincze O,Tökési N,Oláh J,Hlavanda E,Zotter A,Horváth I,Lehotzky A,Tirián L,Medzihradszky KF,Kovács J,Orosz F,Ovádi J

    更新日期:2006-11-21 00:00:00

  • Identification, tissue distribution, and molecular modeling of novel human isoforms of the key enzyme in sialic acid synthesis, UDP-GlcNAc 2-epimerase/ManNAc kinase.

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    pub_type: 杂志文章


    authors: Yardeni T,Choekyi T,Jacobs K,Ciccone C,Patzel K,Anikster Y,Gahl WA,Kurochkina N,Huizing M

    更新日期:2011-10-18 00:00:00

  • Active site loop motion in triosephosphate isomerase: T-jump relaxation spectroscopy of thermal activation.

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    pub_type: 杂志文章


    authors: Desamero R,Rozovsky S,Zhadin N,McDermott A,Callender R

    更新日期:2003-03-18 00:00:00