Abstract：
:Oxindolealanine-62 lysozyme, formed by reaction with N-bromosuccinimide and purified by using affinity chromatography, was examined in its binding of homologous oligosaccharides of N-acetylglucosamine and in its catalysis of hydrolysis and of transglycosylation of the hexasaccharide of N-acetylglucosamine. Equilibrium binding constants were determined by changes in absorbance or fluorescence associated with ligand binding. Enthalpies of binding were measured calorimetrically. The pattern of variation of delta Go and delta Ho of binding with ligand chain length and pH was different for oxindolealanine-62 lysozyme compared with native lysozyme. These results indicate that the interactions of the ABC region of the active site with substrates are substantially altered by Trp-62 oxidation, more than expected for loss only of the Trp-62 interactions. The partitioning of the glycosyl enzyme intermediate between reaction with a saccharide acceptor (transglycosylation) and reaction with water is unaffected by oxidation of Trp-62. Similarly, the pattern of cleavage of the hexasaccharide, predominantly to tetra- and disaccharide, is unaffected by oxidation of Trp-62. The 2000-fold slower rate of catalytic reaction (relative to free enzyme and substrate) apparently reflects a sterically hindered fit of the substrate into the active site of the modified enzyme and not a special catalytic importance of Trp-62. The geometry of the transition state for oligosaccharide hydrolysis is inferred to be the same for native and oxidized enzymes.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Shrake A,Rupley JAdoi
10.1021/bi00558a022subject
Has Abstractpub_date
1980-08-19 00:00:00pages
4044-51issue
17eissn
0006-2960issn
1520-4995journal_volume
19pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:All eight cysteine residues, C90, C94, C143, C147, C219, C325, C367, and C431, present in transmembrane domains of the Aspergillus nidulans NrtA nitrate transporter protein were altered individually by site-specific mutagenesis. The results indicate that six residues, C90, C147, C219, C325, C367, and C431, are not req...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi047732e
更新日期：2005-04-12 00:00:00
abstract：:Evidence is presented that indicates tropoelastin is derived from a soluble elastin with a molecular weight of 95000. Tropoelastin and its proposed precursor were isolated from the aortas of copper-deficient chicks. Although it is doubtful that the proposed precursor is an initial product of elastin translation, i.e.,...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00585a004
更新日期：1979-09-04 00:00:00
abstract：:The SH3 and SH2 domains of hematopoietic cell kinase (Hck) play important roles in substrate targeting. To identify new components of Hck signaling pathways, we identified proteins that bind to the SH3 domain of Hck (Scott et al. (2002) J. Biol. Chem. 277, 28238). One such protein was ELMO1, the mammalian orthologue o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0500832
更新日期：2005-06-21 00:00:00
abstract：:The structure of isocitrate dehydrogenase (IDH) with a bound complex of isocitrate, NADP+, and Ca2+ was solved at 2.5-A resolution and compared by difference mapping against previously determined enzymatic complexes. Calcium replaces magnesium in the binding of metal-substrate chelate complex, resulting in a substanti...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00087a008
更新日期：1993-09-14 00:00:00
abstract：:Apamin is being studied as a model for the folding mechanism of proteins whose structures are stabilized by disulfide bonds. Apamin consists of 18 amino acid residues and forms a stable structure consisting of a C-terminal alpha-helix and two reverse turns. This structure is stabilized by two disulfide bonds connectin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00133a009
更新日期：1992-05-12 00:00:00
abstract：:A method is described for measuring equilibrium constants of DNA-protein interactions using gel chromatography. This technique has been used to study the sequence-specific interaction of the HinfI restriction endonuclease with DNA. HinfI has a monomeric molecular weight of 31000 and exists as a dimer in its active for...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00333a037
更新日期：1985-06-04 00:00:00
abstract：:Reactivation of tetrameric porcine skeletal muscle lactic dehydrogenase after dissociation and extensive unfolding of the monomers by 6 M guanidine hydrochloride (Gdn . HCl) is characterized by sigmoidal kinetics, indicating a complex mechanism involving rate-limiting folding and association steps. For analysis of the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00260a007
更新日期：1982-08-17 00:00:00
abstract：:Bacillus thuringiensis (Bt) insecticidal toxins bind to receptors on midgut epithelial cells of susceptible insects, and binding triggers biochemical events that lead to insect mortality. Recently, a 100-kDa aminopeptidase N (APN) was isolated from brush border membrane vesicles (BBMV) of Anopheles quadrimaculatus and...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi801181g
更新日期：2008-10-28 00:00:00
abstract：:The role of lateral diffusion in mitochondrial electron transport has been investigated by measuring the diffusion coefficients for lipid, cytochrome c, and cytochrome oxidase in membranes of giant mitoplasts from cuprizone-fed mice using the technique of fluorescence redistribution after photobleaching (FRAP). The di...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00331a017
更新日期：1985-05-07 00:00:00
abstract：:1H NMR spectroscopy of the isotropically shifted signals in cobalt carboxypeptidase, CoCPD, permits a direct and selective detection of protons belonging to the residues liganded to the metal. The chemical shift of these protons in the free enzyme and enzyme-inhibitor complexes with changing pH monitors the state of i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00130a015
更新日期：1992-04-21 00:00:00
abstract：:A new bifunctional spin-label (BSL) has been synthesized that can be immobilized on the surface of proteins, allowing measurement of rotational motion of proteins by saturation-transfer electron paramagnetic resonance (STEPR). The spin-label contains a photoactivatable azido moiety, a cleavable disulfide, and a nitrox...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00476a014
更新日期：1990-06-19 00:00:00
abstract：:The ability to undergo large-scale domain rearrangements is essential for the substrate-binding function of periplasmic binding proteins (PBPs), which are indispensable for nutrient uptake in Gram-negative bacteria. Crystal structures indicate that PBPs typically adopt either an "open" unliganded configuration or a "c...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi902045p
更新日期：2010-03-09 00:00:00
abstract：:Duplication of a single β-strand that forms part of a β-sheet in photoactive yellow protein (PYP) was found to produce two approximately isoenergetic protein conformations, in which either the first or the second copy of the duplicated β-strand participates in the β-sheet. Whereas one conformation (big-loop) is more s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00445
更新日期：2018-07-17 00:00:00
abstract：:A human genomic DNA library was screened for the gene coding for the gamma chain of fibrinogen by using a human cDNA for the gamma chain as a hybridization probe. The gene was identified in three overlapping recombinant lambda bacteriophage, and its sequence, including the immediate 5' and 3' flanking regions, was det...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00329a041
更新日期：1985-04-09 00:00:00
abstract：:TCDD (dioxin) induces a rapid inflammatory response from 3T3-L1 adipocytes as judged by prominent induction of the mRNA expression of prostaglandin-endperoxide synthase 2 (Cox-2) along with other inflammation markers within 1 h. This action of TCDD is clearly antagonized by cell pretreatment with AACOCF3 (an inhibitor...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi801913w
更新日期：2008-12-30 00:00:00
abstract：:It now appears that photosynthetic prokaryotes and lower eukaryotes possess higher plant phytochrome-like proteins. In this work, a second phytochrome-like gene was isolated, in addition to the recently identified Cph1 phytochrome, from the Synechocystis sp. PCC 6803, and its gene product was characterized photochemic...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi992831r
更新日期：2000-09-05 00:00:00
abstract：:The interaction of the C-terminal octapeptide of cholecystokinin, CCK-8, with the third extracellular loop of human cholecystokinin-A receptor, CCK(A)-R(329-357), has been probed by high-resolution NMR and extensive computer simulations. The structure of CCK(A)-R(329-357) in the presence of dodecylphosphocholine micel...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002659n
更新日期：2001-04-03 00:00:00
abstract：:Phospholipids and sterols play multiple roles in cells. In addition to establishing barriers between compartments, they also provide the matrix for assembly and function of a large variety of catalytic processes. Lipid composition is a highly regulated feature of biological membranes, yet its implications for membrane...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00326
更新日期：2018-07-17 00:00:00
abstract：:Electron-transfer reactions following the formation of P700(+)A1- have been studied in isolated Photosystem I complexes from Synechococcus elongatus between 300 and 5 K by flash absorption spectroscopy. (1) In the range from 300 to 200 K, A1- is reoxidized by electron transfer to the iron-sulfur cluster FX. The rate s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi973182r
更新日期：1998-06-30 00:00:00
abstract：:We have performed comparative studies of the neutral glycosphingolipids synthesized by three human myeloid leukemia cell lines, K562, KG1, and HL-60, which were metabolically labeled with [14C]galactose, to evaluate changes in neutral glycosphingolipid synthesis with myeloid cell differentiation. Individual neutral gl...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00345a034
更新日期：1985-11-19 00:00:00
abstract：:Many bacteria produce isonitrile-containing natural products that are derived from aromatic amino acids. The synthetic clusters that control biosynthesis most commonly encode two enzymes, designated PvcA and PvcB, as well as additional enzymes that direct synthesis of the natural product. The PvcA enzyme installs the ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00255
更新日期：2015-04-28 00:00:00
abstract：:RNA helicase DHX33 has been shown to participate in a variety of cellular activities, including ribosome biogenesis, protein translation, and gene transcription. We and others further discovered that DHX33 is strongly expressed in several types of human cancers and plays important roles in promoting cancer cell prolif...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00166
更新日期：2019-01-29 00:00:00
abstract：:The presence of a N-(2-deoxy-D-erythro pentofuranosyl)urea (henceforth referred to as deoxyribosylurea) residue, ring fragmentation product of a thymine, in a frameshift situation in the sequence 5'd(AGGACCACG).d(CGTGGurTCCT) has been studied by 1H and 31P nuclear magnetic resonance and molecular dynamics. At equilibr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971202j
更新日期：1998-01-27 00:00:00
abstract：:N-(1-Pyrene)maleimide is nonfluorescent in aqueous solution but forms strongly fluorescent adducts with sulfhydryl groups of organic compounds or proteins. The conjugation reactions of N-(1-pyrene)maleimide are relatively fast and can be monitored by the increase in fluorescence intensity of the pyrene chromophore. In...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00658a025
更新日期：1976-06-29 00:00:00
abstract：:α-Synuclein (α-Syn) oligomerization and amyloid formation are associated with Parkinson's disease (PD) pathogenesis. Studying familial α-Syn mutants associated with early onset PD has therapeutic importance. Here we report the aggregation kinetics and other biophysical properties of a newly discovered PD associated Fi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi5010365
更新日期：2014-10-21 00:00:00
abstract：:Human erythrocyte band 3 inhibits glycolytic enzymes, including aldolase, by binding these cytoplasmic enzymes at its N-terminus. Phosphorylation of Y8 disrupts inhibition, and there is evidence that in vivo glycolysis levels in erythrocytes are regulated in part by a phosphorylation/dephosphorylation signaling pathwa...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00051a005
更新日期：1995-12-26 00:00:00
abstract：:The first transmembrane (TM1) helix in the red cell anion exchanger (AE1, Band 3, or SLC4A1) acts as an internal signal anchor that binds the signal recognition particle and directs the nascent polypeptide chain to the endoplasmic reticulum (ER) membrane where it moves from the translocon laterally into the lipid bila...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00966
更新日期：2017-02-07 00:00:00
abstract：:The R2 protein of ribonucleotide reductase catalyzes the dioxygen-dependent one-electron oxidation of Tyr122 at a diiron-carboxylate site. Methane monooxygenase and related hydroxylases catalyze hydrocarbon hydroxylation at diiron sites structurally related to the one in R2. In protein R2, the likely reaction site for...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9806403
更新日期：1998-07-28 00:00:00
abstract：:Cdx and Hox gene families encode homeodomain-containing transcription factors involved in anterior-posterior vertebral patterning. Although Cdx proteins are direct transcriptional regulators of Hox gene expression, both Hox and Cdx proteins are known to interact with other homeodomain transcription factors, leading us...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi301241q
更新日期：2012-12-04 00:00:00
abstract：:Early studies involving purine salvage in Salmonella typhimurium resulted in the isolation and identification of a mutant strain possessing a genetically modified hypoxanthine phosphoribosyl-transferase (HPRT) with enhanced substrate specificity for guanine [Benson, C. E., and Gots, J. S. (1975) J. Bacteriol. 121, 77-...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9720179
更新日期：1998-03-10 00:00:00
