Nucleotide sequence of the gene for the gamma chain of human fibrinogen.

abstract：:RNA pur*pur-pyr and pyr*pur-pyr (pur = purine, pyr = pyrimidine) triple helices consisting of a Watson-Crick base-paired 28mer hairpin duplex and a Hoogsteen base-paired purine or pyrimidine 12mer are targeted with photoactivated cleavage by the metal complex Rh(phen)2phi3+ (phen = phenanthroline, phi = 9, 10-phenanth...

journal_title：Biochemistry

authors： Lim AC,Barton JK

更新日期：1998-06-23 00:00:00

abstract：:Cibacron Blue F3GA dye has been used to probe subtle conformational changes in protein structure associated with the conversion of Escherichia coli glutamine synthetase (GS) between relaxed, taut, oxidized, and dissociated forms. Binding of the dye to each form of the enzyme elicits a different spectral perturbation o...

journal_title：Biochemistry

authors： Federici MM,Chock PB,Stadtman ER

更新日期：1985-01-29 00:00:00

abstract：:We have used 2D 1H NMR to determine the structure of glucagon-like peptide-1-(7-36) amide bound to a dodecylphosphocholine micelle. In this membranelike environment, the peptide hormone is shown to have a structure similar to that observed for glucagon. It consists of an N-terminal random coil segment (residues 1-7), ...

journal_title：Biochemistry

authors： Thornton K,Gorenstein DG

更新日期：1994-03-29 00:00:00

abstract：:The crystal structure of human platelet factor 4 (PF4) has been solved to a resolution of 2.4 A by molecular replacement and refined to an R-factor of 24.1%. The structure consists of four polypeptide chains which form a tetrameric unit. N-terminal residues, previously defined as a random coil or extended loop region,...

journal_title：Biochemistry

authors： Zhang X,Chen L,Bancroft DP,Lai CK,Maione TE

更新日期：1994-07-12 00:00:00

abstract：:Phototropin, a blue-light photoreceptor in plants, has two FMN-binding domains named LOV1 and LOV2. We previously observed temperature-dependent FTIR spectral changes in the C=O stretching region (amide-I vibrational region of the peptide backbone) for the LOV2 domain of Adiantum phytochrome3 (phy3-LOV2), suggesting p...

journal_title：Biochemistry

authors： Iwata T,Nozaki D,Sato Y,Sato K,Nishina Y,Shiga K,Tokutomi S,Kandori H

更新日期：2006-12-26 00:00:00

abstract：:The linkage between substrate and regulatory effector binding to separate sites on allosteric enzymes results in shifts in their sigmoidal kinetics to regulate metabolism. Control of branched chain amino acid biosynthesis in Escherichia coli occurs in part through shifts in the sigmoidal dependence of alpha-ketobutyra...

journal_title：Biochemistry

authors： Eisenstein E,Yu HD,Fisher KE,Iacuzio DA,Ducote KR,Schwarz FP

更新日期：1995-07-25 00:00:00

abstract：:The ability of the low molecular weight lung surfactant-associated proteins, SP-B and SP-C, to alter the thermotropic properties of synthetic multilamellar vesicles was tested using differential scanning calorimetry (DSC). The presence of either SP-B or SP-C in dipalmitoylphosphatidylcholine (DPPC) or dipalmitoylphosp...

journal_title：Biochemistry

authors： Shiffer K,Hawgood S,Haagsman HP,Benson B,Clements JA,Goerke J

更新日期：1993-01-19 00:00:00

abstract：:The effect of DNA supercoiling on a sterically very demanding threading intercalation process is investigated here. We find that the threading rate of a dimeric ruthenium complex into a negatively supercoiled plasmid at low binding density is 2 orders of magnitude higher than into the cleaved linear form. Further satu...

journal_title：Biochemistry

authors： Nordell P,Jansson ET,Lincoln P

更新日期：2009-02-24 00:00:00

abstract：:In the native purple bacterial reaction center (RC), light-driven charge separation utilizes only the A-side cofactors, with the symmetry related B-side inactive. The process is initiated by electron transfer from the excited primary donor (P*) to the A-side bacteriopheophytin (P* --> P+ H(A)-) in approximately 3 ps. ...

journal_title：Biochemistry

authors： Kee HL,Laible PD,Bautista JA,Hanson DK,Holten D,Kirmaier C

更新日期：2006-06-13 00:00:00

abstract：:Replication protein A (RPA) is multisubunit single-stranded DNA-binding protein required for multiple processes in DNA metabolism including DNA replication, DNA repair, and recombination. Human RPA is a stable complex of three subunits of 70, 32, and 14 kDa (RPA70, RPA32, and RPA14, respectively). We examined the stru...

journal_title：Biochemistry

authors： Gomes XV,Henricksen LA,Wold MS

更新日期：1996-04-30 00:00:00

abstract：:Class II MHC glycoproteins bind short (7-25 amino acid) peptides in an extended type II polyproline-like conformation and present them for immune recognition. Because empty MHC is unstable, measurement of the rate of the second-order reaction between peptide and MHC is challenging. In this report, we use dissociation ...

journal_title：Biochemistry

authors： Kasson PM,Rabinowitz JD,Schmitt L,Davis MM,McConnell HM

更新日期：2000-02-08 00:00:00

abstract：:The sex steroid binding protein (SBP) of human pregnancy serum was purified to homogeneity by the sequential use of ammonium sulfate precipitation, affinity chromatography on 5alpha-dihydrotestosterone-17beta-succinyldiaminoethyl-(1,4-butanediol diglycidyl ether)-agarose, and preparative polyacrylamide gel electrophor...

journal_title：Biochemistry

authors： Mickelson KE,Teller DC,Petra PH

更新日期：1978-04-18 00:00:00

abstract：:Proton NMR spectroscopy was applied to myoglobin in the ferric, water-liganded form (metMbH2O) and the apo form (apoMb) to probe the structure and stability of the latter. Proteins from sperm whale and horse skeletal muscles were studied to simplify the spectral assignment task. Nuclear Overhauser effects and the resp...

journal_title：Biochemistry

authors： Cocco MJ,Kao YH,Phillips AT,Lecomte JT

更新日期：1992-07-21 00:00:00

abstract：:Factors which influence the accessibility, or exposure, of the carbohydrate head group of the glycolipid galactosylceramide (GalCer) at the membrane surface have been examined in lipid model membranes using the technique of galactose oxidase-tritiated sodium borohydride labeling. Both the ceramide composition of GalCe...

journal_title：Biochemistry

authors： Stewart RJ,Boggs JM

更新日期：1993-06-01 00:00:00

abstract：:The binding of various forms of vanadate to myosin and myosin subfragment 1 (S-1) was studied by 51V NMR at increasing vanadate concentrations between 0.06 and 1.0 mM. The distribution of the various forms of vanadate in the solution depended on the total concentration of vanadate. At low concentrations, the predomina...

journal_title：Biochemistry

authors： Ringel I,Peyser YM,Muhlrad A

更新日期：1990-09-25 00:00:00

abstract：:Sedimentation equilibrium and sedimentation velocity measurements demonstrate that turkey gizzard caldesmon is an elongated molecule of molecular mass 75 +/- 2 kDa. The frictional ratio (2.14) is consistent with a prolate ellipsoid of axial ratio 24, corresponding to an apparent length and width of 516 and 21.5 A, res...

journal_title：Biochemistry

authors： Malencik DA,Ausio J,Byles CE,Modrell B,Anderson SR

更新日期：1989-10-03 00:00:00

abstract：:Thermodynamic parameters of helix formation were measured spectroscopically for 16 oligoribonucleotides containing either internal GU mismatches or the corresponding AU pairs. Internal GU mismatches stabilize each helix, but not as much as the corresponding AU pairs. The differences in the enthalpy and entropy changes...

journal_title：Biochemistry

authors： Sugimoto N,Kierzek R,Freier SM,Turner DH

更新日期：1986-09-23 00:00:00

abstract：:Two chimeric receptors were constructed by transposing the coding regions for the putative N-terminal domains of the human calcitonin (hCTR) and glucagon (hGGR) receptors. These receptors were stably expressed as glycosylated proteins with molecular masses of 80 kDa for the calcitonin receptor N-terminus chimera (NtCT...

journal_title：Biochemistry

authors： Stroop SD,Kuestner RE,Serwold TF,Chen L,Moore EE

更新日期：1995-01-24 00:00:00

abstract：:The data and approach reported in paper I (Hanlon et al., 1975, preceding paper) have been used to calculate the fractional changes in secondary structure of calf thymus deoxyribonucleic acid which occur in aqueous solutions as a function of the concentration of NaCl, KCl, LiCl, CsCl, and NH4Cl. There is a continuous ...

journal_title：Biochemistry

authors： Wolf B,Hanlon S

更新日期：1975-04-22 00:00:00

abstract：:Two new inhibitors in which the terminal α-carboxyl groups of Z-Ala-Ala-Phe-COOH and Z-Ala-Pro-Phe-COOH have been replaced with a proton to give Z-Ala-Ala-Phe-H and Z-Ala-Pro-Phe-H, respectively, have been synthesized. Using these inhibitors, we estimate that for α-chymotrypsin and subtilisin Carlsberg the terminal ca...

journal_title：Biochemistry

authors： Petrillo T,O'Donohoe CA,Howe N,Malthouse JP

更新日期：2012-08-07 00:00:00

abstract：:A procedure for the preparation of coupling factor 1 (F1) from Escherichia coli lacking subunits delta and epsilon is described. Using chloroform and dimethyl sulfoxide, we can isolate F1 containing only subunits alpha, beta, and gamma [F1(alpha beta gamma)] directly from membrane vesicles in 10-mg quantities. Pure an...

journal_title：Biochemistry

authors： Tuttas-Dörschug R,Hanstein WG

更新日期：1989-06-13 00:00:00

abstract：:A new procedure to cleave tryptophanyl peptide bonds in high yield is reported. The method involves treatment of the S-alkylated protein with o-iodosobenzoic acid. The procedure is highly selective for tryptophan and does not modify tyrosine or histidine, but may convert methionine to its sulfoxide derivative. The yie...

journal_title：Biochemistry

authors： Mahoney WC,Hermodson MA

更新日期：1979-08-21 00:00:00

abstract：:The interaction of concanavalin A (Con A) with isolated adipocytes was studied using Con A-Sepharose beads in the affinity binding buoyant density method previously used to study insulin receptors. Free Con A-Sepharose beads could be separated from the bound beads (cell-bead complexes) by sedimentation of the high den...

journal_title：Biochemistry

authors： Katzen HM,Soderman DD

更新日期：1975-06-03 00:00:00

abstract：:The kinetics of binding of chicken cystatin to papain were studied by stopped-flow fluorometry under pseudo-first-order conditions, i.e., with an excess of inhibitor. All reactions showed first-order behavior, and the observed pseudo-first-order rate constant increased linearly with the cystatin concentration up to th...

journal_title：Biochemistry

authors： Björk I,Alriksson E,Ylinenjärvi K

更新日期：1989-02-21 00:00:00

abstract：:In the course of measuring the concentrations of retinoic acids (RA) in bovine plasma, a major peak was observed which comigrated with 9-cis-RA on normal-phase high-performance liquid chromatography. Rechromatography of this retinoic on reverse-phase high-performance liquid chromatography showed that it was distinct f...

journal_title：Biochemistry

authors： Horst RL,Reinhardt TA,Goff JP,Nonnecke BJ,Gambhir VK,Fiorella PD,Napoli JL

更新日期：1995-01-31 00:00:00

abstract：:A recombinant form of Rhodobacter sphaeroides phosphoribulokinase (form I; NADH dependent) has been expressed in and purified to homogeneity from Escherichia coli that harbor the prkA gene in the plasmid pKP1565b. Restriction digestion of the phosphoribulokinase-encoding plasmid produces a tractable 450 bp fragment th...

journal_title：Biochemistry

authors： Sandbaken MG,Runquist JA,Barbieri JT,Miziorko HM

更新日期：1992-04-14 00:00:00

abstract：:Expression of the Tn21 mercury-resistance (mer) locus is controlled by the merR gene product, which represses mer structural gene (merTPCAD) transcription in the absence of mercuric ion [Hg(II)] and activates it in the presence of Hg(II). In vivo DNA methylation of the mer regulatory region (merOP) shows that, with or...

journal_title：Biochemistry

authors： Heltzel A,Lee IW,Totis PA,Summers AO

更新日期：1990-10-16 00:00:00

abstract：:Calcium-saturated calmodulin (CaM) binds and influences the activity of a varied collection of target proteins in most cells. This promiscuity underlies the role of CaM as a shared participant in calcium-dependent signal transduction pathways but imposes a handicap on popular CaM-based calcium biosensors, which displa...

journal_title：Biochemistry

authors： Green DF,Dennis AT,Fam PS,Tidor B,Jasanoff A

更新日期：2006-10-17 00:00:00

abstract：:Structural analyses of bacterial thymidylate synthases (TSs) implicate a serine residue corresponding to Ser216 in human TS in hydrogen bond networks that are involved in binding of the nucleotide substrate, 2'-deoxyuridylate (dUMP), and that stabilize a beta-bulge in the protein. Utilizing site-directed mutagenesis, ...

journal_title：Biochemistry

authors： Williams AW,Dunlap RB,Berger SH

更新日期：1998-05-19 00:00:00

abstract：:Prolyl oligopeptidase B from Galerina marginata (GmPOPB) has recently been discovered as a peptidase capable of breaking and forming peptide bonds to yield a cyclic peptide. Despite the relevance of prolyl oligopeptidases in human biology and disease, a kinetic analysis pinpointing rate-limiting steps for a member of ...

journal_title：Biochemistry

authors： Czekster CM,Naismith JH

更新日期：2017-04-18 00:00:00