Chimeric human calcitonin and glucagon receptors reveal two dissociable calcitonin interaction sites.

abstract：:The circulating protein transthyretin (TTR) can unfold, oligomerize, and form highly structured amyloid fibrils that are deposited in tissues, causing organ damage and disease. This pathogenic process is caused by a heritable TTR point mutation in cases of familial TTR-related amyloidosis or wild-type TTR in cases of ...

journal_title：Biochemistry

authors： Greene MJ,Klimtchuk ES,Seldin DC,Berk JL,Connors LH

更新日期：2015-01-20 00:00:00

abstract：:Despite many studies suggesting the role of enolpyruvate as a bound intermediate in the pyruvate kinase reaction, direct evidence for it has been lacking. By use of a combination of chemical trapping and isolation of a derivative, significant amounts of enzyme-bound enolpyruvate have now been demonstrated. The method ...

journal_title：Biochemistry

authors： Seeholzer SH,Jaworowski A,Rose IA

更新日期：1991-01-22 00:00:00

abstract：:Neuronal specification is regulated by the activity of transcription factors containing the basic helix-loop-helix motif (bHLH); these regulating proteins include, among others, the neurogenin (Ngn) family, related to the atonal family of genes. Neurogenin 1 (NGN1) is a 237-residue protein that contains a bHLH domain ...

journal_title：Biochemistry

authors： Aguado-Llera D,Goormaghtigh E,de Geest N,Quan XJ,Prieto A,Hassan BA,Gómez J,Neira JL

更新日期：2010-03-02 00:00:00

abstract：:Two major neutral glyceroglucolipids (A and B) have been isolated from lipid extract of human gastric content by the procedure involving column fractionation on DEAE-Sephadex, silicic acid, and thin-layer chromatography. Both glycolipids contained glucose, glyceryl ethers, and fatty acids. The structures of these glyc...

journal_title：Biochemistry

authors： Slomiany BL,Slomiany A,Glass GB

更新日期：1977-09-06 00:00:00

abstract：:The multicatalytic proteinase complex (MPC, proteasome) is composed of 28 subunits organized into four rings surrounding a water-filled canal. The catalytic centers face the inner canal confining protein substrates to an enclosed space. Experimental findings obtained with MPC from archaebacteria suggest that degradati...

journal_title：Biochemistry

authors： Wang R,Chait BT,Wolf I,Kohanski RA,Cardozo C

更新日期：1999-11-02 00:00:00

abstract：:A minor fraction of plasma high-density lipoprotein (pre beta-1 HDL) has been shown to promote cholesterol efflux from peripheral cell membranes [Castro, G. R., & Fielding, C. J. (1988) Biochemistry 27, 25-29]. When isolated native plasma is incubated at 37 degrees C, this fraction is specifically decreased. On the ot...

journal_title：Biochemistry

authors： Miida T,Kawano M,Fielding CJ,Fielding PE

更新日期：1992-11-17 00:00:00

abstract：:Electron paramagnetic resonance studies have indicated that nitrosodisulfonate binds to pig heart citrate synthase. Titration of the enzyme with nitrosodisulfonate revealed several binding sites for the probe per subunit with one site (KD approximately 0.1 mM) having a greater affinity than the others. The substrate, ...

journal_title：Biochemistry

authors： Weidman SW,Drysdale GR

更新日期：1979-08-21 00:00:00

abstract：:The translocation (T) domain of diphtheria toxin plays a critical role in moving the catalytic domain across the endosomal membrane. Translocation/insertion is triggered by a decrease in pH in the endosome where conformational changes of T domain occur through several kinetic intermediates to yield a final trans-membr...

journal_title：Biochemistry

authors： Li J,Rodnin MV,Ladokhin AS,Gross ML

更新日期：2014-11-04 00:00:00

abstract：:The Cry1Ab toxin produced by Bacillus thuringiensis (Bt) exerts insecticidal action upon binding to BT-R1, a cadherin receptor localized in the midgut epithelium of the tobacco hornworm Manduca sexta [Dorsch, J. A., Candas, M., Griko, N. B., Maaty, W. S., Midboe, E. G., Vadlamudi, R. K., and Bulla, L. A., Jr. (2002) C...

journal_title：Biochemistry

authors： Griko NB,Rose-Young L,Zhang X,Carpenter L,Candas M,Ibrahim MA,Junker M,Bulla LA Jr

更新日期：2007-09-04 00:00:00

abstract：:The mechanism by which the contractile machinery of muscle is assembled and maintained is not well-understood. Members of the cysteine-rich protein (CRP) family have been implicated in these processes. Three vertebrate CRPs (CRP1-3) that exhibit developmentally regulated muscle-specific expression have been identified...

journal_title：Biochemistry

authors： Yao X,Pérez-Alvarado GC,Louis HA,Pomiès P,Hatt C,Summers MF,Beckerle MC

更新日期：1999-05-04 00:00:00

abstract：:Kinetic parameters for triosephosphate isomerase catalysis of the elimination reaction of an equilibrium mixture of dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (DGAP) to form methylglyoxal and phosphate ion are reported for the enzyme from rabbit muscle. Pseudo-first-order rate constants for the...

journal_title：Biochemistry

authors： Richard JP

更新日期：1991-05-07 00:00:00

abstract：:Splicing factor RBM10 and its close homologues RBM5 and RBM6 govern the splicing of oncogenes such as Fas, NUMB, and Bcl-X. The molecular architecture of these proteins includes zinc fingers (ZnFs) and RNA recognition motifs (RRMs). Three of these domains in RBM10 that constitute the RNA binding part of this splicing ...

journal_title：Biochemistry

authors： Serrano P,Hammond JA,Geralt M,Wüthrich K

更新日期：2018-03-13 00:00:00

abstract：:The solution structure of an alpha-insect toxin from Buthus martensii Karsch, BmKalphaIT01, has been determined by two-dimensional NMR spectroscopy and molecular modeling techniques. Combining the sequence homology comparison and toxicity bioassays, BmKalphaIT01 has been suggested to be a natural mutant of alpha-insec...

journal_title：Biochemistry

authors： Tong X,Zhu J,Ma Y,Chen X,Wu G,He F,Cao C,Wu H

更新日期：2007-10-09 00:00:00

abstract：:The crystal structure and spectroscopic properties of the periplasmic penta-heme cytochrome c nitrite reductase (NrfA) of Escherichia coli are presented. The structure is the first for a member of the NrfA subgroup that utilize a soluble penta-heme cytochrome, NrfB, as a redox partner. Comparison to the structures of ...

journal_title：Biochemistry

authors： Bamford VA,Angove HC,Seward HE,Thomson AJ,Cole JA,Butt JN,Hemmings AM,Richardson DJ

更新日期：2002-03-05 00:00:00

abstract：:A new rapid procedure for purifying 10-formyltetrahydrofolate dehydrogenase results in 90 mg of pure enzyme from two rabbit livers. This abundant liver enzyme is known to bind its product tetrahydropteroylpentaglutamate (H4PteGlu5) so tightly that it does not dissociate during size exclusion chromatography. 10-Formylt...

journal_title：Biochemistry

authors： Kim DW,Huang T,Schirch D,Schirch V

更新日期：1996-12-10 00:00:00

abstract：:Homology modeling was used to identify two particular residues, Glu175 and Ala176, in Pseudomonas stutzeri phosphite dehydrogenase (PTDH) as the principal determinants of nicotinamide cofactor (NAD(+) and NADP(+)) specificity. Replacement of these two residues by site-directed mutagenesis with Ala175 and Arg176 both s...

journal_title：Biochemistry

authors： Woodyer R,van der Donk WA,Zhao H

更新日期：2003-10-14 00:00:00

abstract：:The substrate specificities of two incorrectly annotated enzymes belonging to cog3964 from the amidohydrolase superfamily were determined. This group of enzymes are currently misannotated as either dihydroorotases or adenine deaminases. Atu3266 from Agrobacterium tumefaciens C58 and Oant2987 from Ochrobactrum anthropi...

journal_title：Biochemistry

authors： Ornelas A,Korczynska M,Ragumani S,Kumaran D,Narindoshvili T,Shoichet BK,Swaminathan S,Raushel FM

更新日期：2013-01-08 00:00:00

abstract：:The importance of a specific hydrogen bond between thermolysin and a phosphonamidate inhibitor, Z-NHCH2-PO(O-)-Leu-Leu (1) [Bartlett, P. A., & Marlowe, C. K. (1987) Science (Washington D.C.) 235, 569-571], has been reevaluated. We have determined the inhibition constants (binding free energies) for thermolysin of phos...

journal_title：Biochemistry

authors： Grobelny D,Goli UB,Galardy RE

更新日期：1989-06-13 00:00:00

abstract：:The reaction between chymotrypsin and N-acetyl-L-phenylalanine p-nitroanilide has been studied at subzero temperatures in fluid aqueous dimethyl sulfoxide solvent. Following initiation of the reaction at temperatures as low as -90 degrees C, a series of four reactions prior to the normal rate-limiting step (acylation)...

journal_title：Biochemistry

authors： Fink AL

更新日期：1976-04-06 00:00:00

abstract：:This paper reports on a solution NMR characterization of the sequence selectivity and metal ion specificity in chromomycin-DNA oligomer complexes in the presence of divalent cations. The sequence selectivity studies have focused on chromomycin complexes with the self-complementary d(A1-A2-G3-G4-C5-C6-T7-T8) duplex con...

journal_title：Biochemistry

authors： Gao XL,Patel DJ

更新日期：1990-12-11 00:00:00

abstract：:The protease prostate-specific antigen (PSA) is a marker widely used clinically for monitoring prostatic malignancies. Under normal conditions, this enzyme is mainly involved in the post ejaculation degradation of the major human seminal protein, the seminal plasma motility inhibitor precursor/semenogelin I (SPMIP/SgI...

journal_title：Biochemistry

authors： Robert M,Gibbs BF,Jacobson E,Gagnon C

更新日期：1997-04-01 00:00:00

abstract：:A series of antisense oligonucleotides (ASOs) were synthesized and tested to define the best target sites within an RNA transcript of collagen for effective inhibition of expression. The test system consisted of mouse NIH 3T3 fibroblasts that were stably transfected with a human minigene for procollagen I so that the ...

journal_title：Biochemistry

authors： Laptev AV,Lu Z,Colige A,Prockop DJ

更新日期：1994-09-13 00:00:00

abstract：:A wide range of two-dimensional 1H NMR experiments have been used to completely assign the 500-MHz 1H NMR spectrum of recombinant Ca2+-saturated bovine calbindin D9k (76 amino acids, Mr = 8500). In solution, calbindin D9k exists as an equilibrium mixture of isoforms with trans (75%) and cis (25%) isomers of the peptid...

journal_title：Biochemistry

authors： Kördel J,Forsén S,Chazin WJ

更新日期：1989-08-22 00:00:00

abstract：:A putative catalytic triad consisting of tyrosine, serine, and lysine residues was identified in the ketoreductase (KR) domains of modular polyketide synthases (PKSs) based on homology modeling to the short chain dehydrogenase/reductase (SDR) superfamily of enzymes. This was tested by constructing point mutations for ...

journal_title：Biochemistry

authors： Reid R,Piagentini M,Rodriguez E,Ashley G,Viswanathan N,Carney J,Santi DV,Hutchinson CR,McDaniel R

更新日期：2003-01-14 00:00:00

abstract：:The free solution electrophoretic mobilities and thermal stabilities of hairpins formed by two complementary 26-nucleotide oligomers have been measured by capillary electrophoresis. The oligomers are predicted to form molecular beacon-like hairpins with 5 bp stems and 16 nucleotides in the loop. One hairpin, called ha...

journal_title：Biochemistry

authors： Chang CY,Stellwagen NC

更新日期：2011-10-25 00:00:00

abstract：:Restrictocin is a small basic protein produced by the fungus Aspergillus restrictus. It potently inhibits protein synthesis in eukaryotic cells by specifically cleaving a single phosphodiester bond in 28S rRNA. A histidine residue at position 49 in restrictocin has been implicated in its active site. A mutant of restr...

journal_title：Biochemistry

authors： Nayak SK,Batra JK

更新日期：1997-11-04 00:00:00

abstract：:The biosynthesis of the C ring of the antitumor antibiotic agent, tomaymycin, is proposed to proceed through five enzyme-catalyzed steps from l-tyrosine. The genes encoding these enzymes have recently been cloned and their functions tentatively assigned, but there is limited biochemical evidence supporting the assignm...

journal_title：Biochemistry

authors： Burks EA,Yan W,Johnson WH Jr,Li W,Schroeder GK,Min C,Gerratana B,Zhang Y,Whitman CP

更新日期：2011-09-06 00:00:00

abstract：:In order to more clearly define the structure of human plasma fibronectin (PFn) under physiologic buffer conditions, we determined the mean harmonic rotational relaxation times (rho H) of PFn and the thrombin-derived 190/170-kDa PFn fragment using steady-state fluorescence polarization. These measurements utilized the...

journal_title：Biochemistry

authors： Benecky MJ,Kolvenbach CG,Wine RW,DiOrio JP,Mosesson MW

更新日期：1990-03-27 00:00:00

abstract：:The alpha-helices of coiled-coil proteins are predominantly parallel, in contrast to the general preference for an antiparallel orientation of interacting alpha-helices found in globular proteins. One intriguing exception is the antiparallel, two-stranded coiled coil comprising the long helical arm of the bacterial se...

journal_title：Biochemistry

authors： Oakley MG,Kim PS

更新日期：1997-03-04 00:00:00

abstract：:Western blot analysis, using a polyclonal antibody to the 240-kDa endogenous inhibitor of the 20 S proteasome, revealed that the inhibitor is a component of the 26 S complex. Although isolated inhibitor displayed a single 40-kDa band on SDS-PAGE, the antibody detected a 55-kDa component in the 26 S proteasome complex....

journal_title：Biochemistry

authors： Li XS,Etlinger JD

更新日期：1992-12-08 00:00:00