Univalent binding of the Cry1Ab toxin of Bacillus thuringiensis to a conserved structural motif in the cadherin receptor BT-R1.

abstract：:The thermal denaturation of caricain (the most alkaline of papain-related proteinases) was studied in acid media. Under all conditions tested, caricain denatured irreversibly following a single first-order reaction that involves simultaneous loss of secondary and tertiary structures. Besides, variation of the rate con...

journal_title：Biochemistry

authors： López-Arenas L,Solís-Mendiola S,Hernández-Arana A

更新日期：1999-11-30 00:00:00

abstract：:The stability and folding kinetics of wild-type and a mutant staphylococcal nuclease (SNase) at neutral pH are significantly perturbed by the presence of moderate to high concentrations of salts. Very substantial increases in stability toward thermal and urea denaturation were observed; for example, 0.4 M sodium sulfa...

journal_title：Biochemistry

authors： Nishimura C,Uversky VN,Fink AL

更新日期：2001-02-20 00:00:00

abstract：:The causes of the salting-in of beta-lactoglobulin by glycine and NaCl, a solubility behavior contrary to expectations, were probed by a detailed study of the interactions between these solvent components and the protein. The preferential interactions of beta-lactoglobulin with solvent components in aqueous glycine an...

journal_title：Biochemistry

authors： Arakawa T,Timasheff SN

更新日期：1987-08-11 00:00:00

abstract：:Structural properties of the isolated extrinsic regulatory 33 kDa protein of the water-oxidizing complex were analyzed at different pH values. It was found that (a) titrations of the buffer capacity reveal a characteristic hysteresis effect that is unique for the 33 kDa subunit and is not observed for the other extrin...

journal_title：Biochemistry

authors： Shutova T,Irrgang KD,Shubin V,Klimov VV,Renger G

更新日期：1997-05-27 00:00:00

abstract：:Homeodomain-only protein (HOP) is an 8-kDa transcriptional corepressor that is essential for the normal development of the mammalian heart. Previous studies have shown that HOP, which consists entirely of a putative homeodomain, acts downstream of Nkx2.5 and associates with the serum response factor (SRF), repressing ...

journal_title：Biochemistry

authors： Kook H,Yung WW,Simpson RJ,Kee HJ,Shin S,Lowry JA,Loughlin FE,Yin Z,Epstein JA,Mackay JP

更新日期：2006-09-05 00:00:00

abstract：:The electrostatic binding strength of water-soluble proteins having either an excess positive (cytochrome c) or negative (beta-lactoglobulin) electric charge to oppositely charged supported planar bilayers (SPBs) was studied as a function of the bilayer phase state (fluid or gel phase) by IR-ATR spectroscopy. The bila...

journal_title：Biochemistry

authors： Käsbauer M,Bayerl TM

更新日期：1999-11-16 00:00:00

abstract：:Bioluminescence with luciferase-luciferin pairs is an attractive method for surveying cells in live tissues and whole organisms. Recent advances in luciferin chemistry and luciferase engineering are further expanding the scope of the technology. It is now possible to spy on cells in a variety of deep tissues and visua...

journal_title：Biochemistry

authors： Rathbun CM,Prescher JA

更新日期：2017-10-03 00:00:00

abstract：:We have examined the binding of 125I-labeled human and mouse epidermal growth factors (EGF) to human alpha 2-macroglobulin (alpha 2M). In the presence of human neutrophil elastase, both mouse and human EGF bound to alpha 2M, whereas little binding was found to native alpha 2M. Binding was found to be predominantly cov...

journal_title：Biochemistry

authors： Gettins PG,Crews BC

更新日期：1993-08-10 00:00:00

abstract：:Single-strand-specific nucleases are a diverse and important group of enzymes that are able to cleave a variety of DNA structures present in duplex molecules. Nuclease SP, an enzyme from spinach, has been purified to apparent homogeneity, allowing for the unambiguous characterization of a number of its physical proper...

journal_title：Biochemistry

authors： Strickland JA,Marzilli LG,Puckett JM Jr,Doetsch PW

更新日期：1991-10-08 00:00:00

abstract：:X-ray absorption spectroscopy on the minimal copper-regulatory domains of the two copper-regulated transcription factors (Ace1 and Mac1) in Saccharomyces cerevisiae revealed the presence of a remarkably similar polycopper cluster in both proteins. The Cu-regulatory switch motif of Mac1 consisting of the C-terminal fir...

journal_title：Biochemistry

authors： Brown KR,Keller GL,Pickering IJ,Harris HH,George GN,Winge DR

更新日期：2002-05-21 00:00:00

abstract：:Avoidance of over-reduction of the chloroplast ferredoxin pool is of paramount importance for plants in avoiding oxidative stress. The redox state of this pool can be controlled through regulation of the thylakoid electron transport chain. A model is presented for regulation of this chain via a thiol reduction mechani...

journal_title：Biochemistry

authors： Johnson GN

更新日期：2003-03-18 00:00:00

abstract：:X-ray crystallographic studies have shown that DNA binding by human polymerase beta (pol beta) occurs primarily through two structurally and sequentially homologous helix-hairpin-helix (HhH) motifs, one in the fingers subdomain and the other in the 8-kDa domain [Pelletier, H., Sawaya, M. R., Wolfle, W., Wilson, S. H.,...

journal_title：Biochemistry

authors： Pelletier H,Sawaya MR

更新日期：1996-10-01 00:00:00

abstract：:Coiled coils (CCs) are among the best-understood protein folds. Nonetheless, there are gaps in our knowledge of CCs. Notably, CCs are likely to be structurally more dynamic than often considered. Here, we explore this in an abundant class of CCs, parallel dimers, focusing on polar asparagine (Asn) residues in the hydr...

journal_title：Biochemistry

authors： Thomas F,Niitsu A,Oregioni A,Bartlett GJ,Woolfson DN

更新日期：2017-12-19 00:00:00

abstract：:Pseudokinases play important roles in signal transduction and cellular processes similar to those of catalytically competent kinases. However, pseudokinase pharmacological tractability and conformational space accessibility are poorly understood. Pseudokinases have only recently been suggested to adopt "inactive" conf...

journal_title：Biochemistry

authors： Smith RHB,Khan ZM,Ung PM,Scopton AP,Silber L,Mack SM,Real AM,Schlessinger A,Dar AC

更新日期：2021-01-13 00:00:00

abstract：:Tetrachlorohydroquinone dehalogenase catalyzes the replacement of chlorine atoms on tetrachlorohydroquinone and trichlorohydroquinone with hydrogen atoms during the biodegradation of pentachlorophenol by Sphingomonas chlorophenolica. The sequence of the active site region of tetrachlorohydroquinone dehalogenase is ver...

journal_title：Biochemistry

authors： Anandarajah K,Kiefer PM Jr,Donohoe BS,Copley SD

更新日期：2000-05-09 00:00:00

abstract：:The unfolding and dissociation of the tetrameric enzyme fructose-1,6-bisphosphatase from pig kidney by guanidine hydrochloride have been investigated at equilibrium by monitoring enzyme activity, ANS binding, intrinsic (tyrosine) protein fluorescence, exposure of thiol groups, fluorescence of extrinsic probes (AEDANS,...

journal_title：Biochemistry

authors： Reyes AM,Ludwig HC,Yañez AJ,Rodríguez PH,Slebe JC

更新日期：2003-06-17 00:00:00

abstract：:β-Lactam antibiotics have faced obsolescence with the emergence of methicillin-resistant Staphylococcus aureus (MRSA). A complex set of events ensues upon exposure of MRSA to these antibiotics, which culminates in proteolysis of BlaI or MecI, two gene repressors, and results in the induction of resistance. We report s...

journal_title：Biochemistry

authors： Blázquez B,Llarrull LI,Luque-Ortega JR,Alfonso C,Boggess B,Mobashery S

更新日期：2014-03-18 00:00:00

abstract：:To determine whether ligand-receptor interactions could engender the selective uptake by deficient cells of enzyme-laden liposomes, aggregated human IgG was used to coat liposomes which had previously trapped purified hexosaminidase A (Hex A). By a new, high-yield procedure, Hex A was purified 7000-fold from human pla...

journal_title：Biochemistry

authors： Cohen CM,Weissmann G,Hoffstein S,Awasthi YC,Srivastava SK

更新日期：1976-01-27 00:00:00

abstract：:Fluorescence studies of the binding of IKe gene 5 protein to various polynucleotides were performed to obtain insight into the question as to what extent the binding characteristics of the gene 5 proteins of the IKe and M13 phages resemble and/or differ from each other. The fluorescence of IKe gene 5 protein is quench...

journal_title：Biochemistry

authors： de Jong EA,Harmsen BJ,Konings RN,Hilbers CW

更新日期：1987-04-07 00:00:00

abstract：:A previously unknown polypeptide myotoxin, designated myotoxin a, was isolated for the first time from prairie rattlesnake (Crotalus viridis viridis) venom. Electrophoretic homogeneity of myotoxin a was shown in beta-alanine disc gel polyacrylamide gel electrophoresis and in isoelectric focusing gel electrophoresis. M...

journal_title：Biochemistry

authors： Cameron DL,Tu AT

更新日期：1977-05-31 00:00:00

abstract：:Eukaryotic transfer RNA precursors (pre-tRNAs) contain a 5' leader preceding the aminoacyl acceptor stem and a 3' trailer extending beyond this stem. An early step in pre-tRNA maturation is removal of the 5' leader by the endoribonuclease, RNase P. Extensive pairing between leader and trailer sequences has previously ...

journal_title：Biochemistry

authors： Ziehler WA,Day JJ,Fierke CA,Engelke DR

更新日期：2000-08-15 00:00:00

abstract：:The selective paramagnetic relaxation of oligonucleotide protons of d(GTGCAC)2 by delta- and lambda-Ni(phen)3(3+) and delta- and lambda-Cr(phen)3(3+) has been examined to obtain some structural insight into the noncovalent binding of tris(phenanthroline) metal complexes to DNA. The experiments demonstrate that the rel...

journal_title：Biochemistry

authors： Rehmann JP,Barton JK

更新日期：1990-02-20 00:00:00

abstract：:Yah1p, an [Fe 2S 2]-containing ferredoxin located in the matrix of Saccharomyces cerevisiae mitochondria, functions in the synthesis of Fe/S clusters and heme a prosthetic groups. EPR, Mossbauer spectroscopy, and electron microscopy were used to characterize the Fe that accumulates in Yah1p-depleted isolated intact mi...

journal_title：Biochemistry

authors： Miao R,Martinho M,Morales JG,Kim H,Ellis EA,Lill R,Hendrich MP,Münck E,Lindahl PA

更新日期：2008-09-16 00:00:00

abstract：:Two chimeric receptors were constructed by transposing the coding regions for the putative N-terminal domains of the human calcitonin (hCTR) and glucagon (hGGR) receptors. These receptors were stably expressed as glycosylated proteins with molecular masses of 80 kDa for the calcitonin receptor N-terminus chimera (NtCT...

journal_title：Biochemistry

authors： Stroop SD,Kuestner RE,Serwold TF,Chen L,Moore EE

更新日期：1995-01-24 00:00:00

abstract：:The major phospholipids, phosphatidylcholine and spingomyelin, of low density lipoprotein (LDL) are accessible to small amounts of Pr3+, suggesting that the head groups of all mobile phospholipids are on the surface of the particle in contact with the aqueous medium. The major source of the nuclear Overhauser effect e...

journal_title：Biochemistry

authors： Yeagle PL,Martin RB,Pottenger L,Langdon RG

更新日期：1978-07-11 00:00:00

abstract：:Pokeweed antiviral protein (PAP) is a single-chain ribosome inactivating protein (RIP) that binds to ribosomes and depurinates the highly conserved alpha-sarcin/ricin loop (SRL) of the large subunit rRNA. Catalytic depurination of a specific adenine has been proposed to result in translation arrest and cytotoxicity. H...

journal_title：Biochemistry

authors： Parikh BA,Baykal U,Di R,Tumer NE

更新日期：2005-02-22 00:00:00

abstract：:The recruitment and organization of clathrin at endocytic sites first to form coated pits and then clathrin-coated vesicles depend on interactions between the clathrin N-terminal domain (TD) and multiple clathrin binding sequences on the cargo adaptor and accessory proteins that are concentrated at such sites. Up to f...

journal_title：Biochemistry

authors： Zhuo Y,Cano KE,Wang L,Ilangovan U,Hinck AP,Sousa R,Lafer EM

更新日期：2015-04-28 00:00:00

abstract：:Light-induced GTP-dependent scattering changes are studied in suspensions of retinal disc membranes to which one or both of the purified proteins involved in the phototransduction mechanism (G-protein and cGMP phosphodiesterase) are reassociated; a scattering change which depends on the presence of both G-protein (G) ...

journal_title：Biochemistry

authors： Bennett N,Clerc A

更新日期：1992-02-18 00:00:00

abstract：:S-Adenosylmethionine decarboxylase is a pyruvate-dependent enzyme. The enzyme forms a Schiff base with substrate, S-adenosylmethionine, through the pyruvoyl moiety. This facilitates the release of CO2 from the substrate, which must then be protonated on the alpha carbon in order to permit hydrolysis of the Schiff base...

journal_title：Biochemistry

authors： Xiong H,Stanley BA,Pegg AE

更新日期：1999-02-23 00:00:00

abstract：:Alpha2u-globulin is a male rat liver protein under multihormonal control which represents approximately 1% of hepatic protein synthesis. We have measured the rate of transcription of the alpha2u-globulin gene using a nuclear cell-free transcriptional system with mercurated CTP as substrate for the endogenous RNA polym...

journal_title：Biochemistry

authors： Chan KM,Kurtz DT,Feigelson P

更新日期：1978-07-25 00:00:00