Structures of the cuprous-thiolate clusters of the Mac1 and Ace1 transcriptional activators.

abstract：:Taq DNA polymerase, one of the first thermostable DNA polymerases to be discovered, has been typecast as a DNA-dependent DNA polymerase commonly employed for PCR. However, Taq polymerase belongs to the same DNA polymerase superfamily as the Molony murine leukemia virus reverse transcriptase and has in the past been sh...

journal_title：Biochemistry

authors： Bhadra S,Maranhao AC,Paik I,Ellington AD

更新日期：2020-12-15 00:00:00

abstract：:Minibrain kinase/dual-specificity tyrosine phosphorylation regulated kinase 1A (MNB/DYRK1A) is a proline/arginine-directed serine/threonine kinase implicated in the learning deficits of Down syndrome. Epigallocatechin-3-gallate (EGCG), the major tea polyphenolic compound, is a potent MNB/DYRK1A inhibitor. In this stud...

journal_title：Biochemistry

authors： Adayev T,Chen-Hwang MC,Murakami N,Wegiel J,Hwang YW

更新日期：2006-10-03 00:00:00

abstract：:Previous studies have established that the mechanism of species-specific sponge cell reaggregation in vitro involves at least three components: a species-specific aggregation factor, its cell surface receptors, and Ca2+ ions. Here we report about the binding of Microciona 125I-labeled aggregation factor to cells and t...

journal_title：Biochemistry

authors： Jumblatt JE,Schlup V,Burger MM

更新日期：1980-03-04 00:00:00

abstract：:Oligonucleotides capable of sequence-specific triple helix formation have been proposed as DNA binding ligands useful for modulation of gene expression and for directed genome modification. However, the effectiveness of such triplex-forming oligonucleotides (TFOs) depends on their ability to bind to their target sites...

journal_title：Biochemistry

authors： Lacroix L,Lacoste J,Reddoch JF,Mergny JL,Levy DD,Seidman MM,Matteucci MD,Glazer PM

更新日期：1999-02-09 00:00:00

abstract：:We previously showed that estrogen-related receptor alpha1 (ERRalpha1) can compete with estrogen receptor alpha (ERalpha) for binding to estrogen response elements (EREs), repressing transcription in the mammary carcinoma cell line MCF-7. Given that ERRalpha1 can function in the absence of ligands and exists as a phos...

journal_title：Biochemistry

authors： Vu EH,Kraus RJ,Mertz JE

更新日期：2007-08-28 00:00:00

abstract：:In reconstituted vesicles above the lipid phase transition temperature, bacteriorhodopsin (BR) undergoes rotational diffusion about an axis perpendicular to the plane of the bilayer [Cherry, R. J., Muller, U., & Schneider, G. (1977) FEBS Lett. 80, 465]. This diffusion narrows the 13C NMR powder line shape of the BR pe...

journal_title：Biochemistry

authors： Lewis BA,Harbison GS,Herzfeld J,Griffin RG

更新日期：1985-08-13 00:00:00

abstract：:Vitamin E is a dietary lipid that is essential for vertebrate health and fertility. The biological activity of vitamin E is thought to reflect its ability to quench oxygen- and carbon-based free radicals and thus to protect the organism from oxidative damage. However, recent reports suggest that vitamin E may also dis...

journal_title：Biochemistry

authors： Valastyan S,Thakur V,Johnson A,Kumar K,Manor D

更新日期：2008-01-15 00:00:00

abstract：:All CG dinucleotides along exons 5-8 of the p53 tumor suppressor gene contain endogenous 5-methylcytosine (MeC). These same sites (e.g., codons 157, 158, 245, 248, and 273) are mutational hot spots in smoking-induced lung cancer. Several groups used the UvrABC endonuclease incision assay to demonstrate that methylated...

journal_title：Biochemistry

authors： Ziegel R,Shallop A,Upadhyaya P,Jones R,Tretyakova N

更新日期：2004-01-20 00:00:00

abstract：:The predominant working hypothesis of Alzheimer's disease is that the proximate pathologic agents are oligomers of the amyloid β-protein (Aβ). "Oligomer" is an ill-defined term. Many different types of oligomers have been reported, and they often exist in rapid equilibrium with monomers and higher-order assemblies. Th...

journal_title：Biochemistry

authors： Hayden EY,Teplow DB

更新日期：2012-08-14 00:00:00

abstract：:The cytochrome aa3-type terminal quinol oxidase of Bacillus subtilis catalyzes the four-electron reduction of dioxygen to water. It resembles the aa3-type cytochrome-c oxidase in using heme A as its active-site chromophores but lacks the CuA center and the cytochrome-c oxidizing activity of the mitochondrial enzyme. W...

journal_title：Biochemistry

authors： Lauraeus M,Wikström M,Varotsis C,Tecklenburg MM,Babcock GT

更新日期：1992-10-20 00:00:00

abstract：:Protein methylation is a common post-translational modification with diverse biological functions. Methyllysine reader proteins are increasingly a focus of epigenetics research and play important roles in regulating many cellular processes. These reader proteins are vital players in development, cell cycle regulation,...

journal_title：Biochemistry

authors： Milosevich N,Hof F

更新日期：2016-03-22 00:00:00

abstract：:The dynamics of the backbone and (some of) the side chains of lac headpiece (1-56; lac HP56) have been studied for the free protein and for its complex with lac half-operator DNA by 15N T1 and T1p relaxation measurements combined with [1H-15N] NOE experiments. For the structurally well-defined part of the free lac HP5...

journal_title：Biochemistry

authors： Slijper M,Boelens R,Davis AL,Konings RN,van der Marel GA,van Boom JH,Kaptein R

更新日期：1997-01-07 00:00:00

abstract：:Misaligned structures can result from strand slippage during DNA replication and, if not repaired, would lead to mutations. Previously, we showed that strand slippage can occur upon misincorporation of a dNTP opposite thymine and cytosine templates, resulting in a misaligned structure with a T- or C-bulge. The formati...

journal_title：Biochemistry

authors： Chi LM,Lam SL

更新日期：2009-12-08 00:00:00

abstract：:The orientation of the myosin neck was monitored using electron paramagnetic resonance (EPR) spectroscopy. Gizzard regulatory light chain was labeled with a nitroxide spin probe and exchanged for the native subunit, located in the myosin neck, in rabbit psoas muscle fibers. The EPR spectra of rigor fibers indicated a ...

journal_title：Biochemistry

authors： Zhao L,Gollub J,Cooke R

更新日期：1996-08-06 00:00:00

abstract：:Biochemical characterization of the inhibition mechanism of Deltalac-acetogenins synthesized in our laboratory indicated that they are a new type of inhibitor of bovine heart mitochondrial NADH-ubiquinone oxidoreductase (complex I) [Murai, M., et al. (2006) Biochemistry 45, 9778-9787]. To identify the binding site of ...

journal_title：Biochemistry

authors： Kakutani N,Murai M,Sakiyama N,Miyoshi H

更新日期：2010-06-15 00:00:00

abstract：:The highly cooperative binding of fd gene 5 to single-stranded DNA was studied kinetically by rapid photo-cross-linking and stopped-flow UV absorption measurements. The observed change in absorbance was shown to be due to the binding by direct evidence of rapid photo-cross-linking of the bound proteins to fd DNA. The ...

journal_title：Biochemistry

authors： Shimamoto N,Utiyama H

更新日期：1983-12-06 00:00:00

abstract：:The inhibitory potency of 3'-azido-3'-deoxythymidine 5'-triphosphate (AZTTP) against HIV-1 reverse transcriptase (HIV-1 RT) has been further evaluated. The results indicate that the previously reported low Ki values for AZTTP against HIV-1 RT (2.35 nM) are due neither to the to the direct tight binding of AZTTP to HIV...

journal_title：Biochemistry

authors： Ma QF,Bathurst IC,Barr PJ,Kenyon GL

更新日期：1992-02-11 00:00:00

abstract：:In the present report we studied the interaction between the skeletal muscle ryanodine receptor and the ubiquitous S100A1 Ca2+ binding protein. S100A1 did not affect equilibrium [3H]ryanodine binding to purified rabbit skeletal muscle terminal cisternae at 100 microM free [Ca2+]. At nanomolar free [Ca2+], however, S10...

journal_title：Biochemistry

authors： Treves S,Scutari E,Robert M,Groh S,Ottolia M,Prestipino G,Ronjat M,Zorzato F

更新日期：1997-09-23 00:00:00

abstract：:RNA binding proteins play an important role in regulating alternative pre-mRNA splicing and in turn cellular gene expression. Many of these RNA binding proteins occur as gene families with members sharing a high degree of primary structure identity and domain organization yet have tissue-specific expression patterns a...

journal_title：Biochemistry

authors： Pina JM,Reynaga JM,Truong AAM,Keppetipola NM

更新日期：2018-07-03 00:00:00

abstract：:Full-length protein tyrosine phosphatase 1C (PTP1C), the catalytic domain of PTP1C (delta PTP1C), and the N-terminal SH2 domain truncated PTP1C (delta NPTP1C) were overexpressed in Escherichia coli and purified to near homogeneity. Various phosphorylated states of the synthetic phosphotyrosyl peptide TRDIYETDYYRK (IRP...

journal_title：Biochemistry

authors： Townley R,Shen SH,Banville D,Ramachandran C

更新日期：1993-12-14 00:00:00

abstract：:Crowding, which characterizes the interior of all living cells, has been shown to dramatically affect biochemical processes, leading to stabilization of compact morphologies, enhanced macromolecular associations, and altered reaction rates. Due to the crowding-mediated shift in binding equilibria toward association, c...

journal_title：Biochemistry

authors： Goobes R,Kahana N,Cohen O,Minsky A

更新日期：2003-03-04 00:00:00

abstract：:Twenty-five different temperature-sensitive point mutations at 20 sites in the lysozyme gene of bacteriophage T4 have been identified. All of the mutations alter amino acid side chains that have lower than average crystallographic thermal factors and reduced solvent accessibility in the folded protein. This suggests t...

journal_title：Biochemistry

authors： Alber T,Sun DP,Nye JA,Muchmore DC,Matthews BW

更新日期：1987-06-30 00:00:00

abstract：:The conformational properties of purine-pyrimidine and pyrimidine-purine dinucleoside monophosphates in which the glycosidic torsion is fixed to congruent to 120 degree by the formation of a covalent link between the base and the sugar ring are explored by 1H NMR spectroscopy in order to obtain information about the s...

journal_title：Biochemistry

authors： Dhingra MM,Sarma RH,Uesugi S,Shida T,Ikehara M

更新日期：1981-08-18 00:00:00

abstract：:The mechanism by which the contractile machinery of muscle is assembled and maintained is not well-understood. Members of the cysteine-rich protein (CRP) family have been implicated in these processes. Three vertebrate CRPs (CRP1-3) that exhibit developmentally regulated muscle-specific expression have been identified...

journal_title：Biochemistry

authors： Yao X,Pérez-Alvarado GC,Louis HA,Pomiès P,Hatt C,Summers MF,Beckerle MC

更新日期：1999-05-04 00:00:00

abstract：:Yeast (Saccharomyces cerevisiae) alcohol dehydrogenase I (ADH1) is the constitutive enzyme that reduces acetaldehyde to ethanol during the fermentation of glucose. ADH1 is a homotetramer of subunits with 347 amino acid residues. A structure for ADH1 was determined by X-ray crystallography at 2.4 Å resolution. The asym...

journal_title：Biochemistry

authors： Raj SB,Ramaswamy S,Plapp BV

更新日期：2014-09-16 00:00:00

abstract：:Matrix metalloproteinase-13 (MMP-13) is important in the pathology of osteoarthritis (OA). Although MMP-13 is considered a therapeutic target for OA, it is unclear how MMP-13 activity is regulated by the system that comprises various proteinases and their inhibitors. MMP-13 neutralizing antibodies could be a useful to...

journal_title：Biochemistry

authors： Naito S,Takahashi T,Onoda J,Yamauchi A,Kawai T,Kishino J,Yamane S,Fujii I,Fukui N,Numata Y

更新日期：2012-11-06 00:00:00

abstract：:p-(Chloromercuri)benzoic acid inhibited the catalytic activity of Bacillus stearothermophilus farnesyl diphosphate synthase (FPP synthase), which contains only two cysteine residues at positions 73 and 289. In order to explore the role of the cysteine residues, either or both of them were replaced with phenylalanine o...

journal_title：Biochemistry

authors： Koyama T,Obata S,Saito K,Takeshita-Koike A,Ogura K

更新日期：1994-10-25 00:00:00

abstract：:Large primary deuterium kinetic isotope effects (1° DKIEs) on enzyme-catalyzed hydride transfer may be observed when the transferred hydride tunnels through the energy barrier. The following 1° DKIEs on kcat/ Km and relative reaction driving force are reported for wild-type and mutant glycerol-3-phosphate dehydrogenas...

journal_title：Biochemistry

authors： Reyes AC,Amyes TL,Richard JP

更新日期：2018-07-24 00:00:00

abstract：:Acyclovir triphosphate, ganciclovir triphosphate and penciclovir triphosphate inhibited DNA polymerases alpha, delta, and epsilon. Each triphosphate preferentially inhibited pol delta, although ganciclovir triphosphate was the most impressive of the three; the Ki for inhibition of pol delta was 2 microM (competitive w...

journal_title：Biochemistry

authors： Ilsley DD,Lee SH,Miller WH,Kuchta RD

更新日期：1995-02-28 00:00:00

abstract：:Tularemia is a severe infectious disease in humans caused by the Gram-negative bacterium Francisella tularensis (Ft). Because of its low infectious dose, high mortality rate, and the threat of its large-scale dissemination in weaponized form, development of vaccines and immunotherapeutics against Ft is essential. Ft l...

journal_title：Biochemistry

authors： Wang Q,Shi X,Leymarie N,Madico G,Sharon J,Costello CE,Zaia J

更新日期：2011-12-20 00:00:00