Bioluminescent Probes for Imaging Biology beyond the Culture Dish.

abstract：:The molecular weight of phosphophoryn, an acidic phosphoprotein unique to dentin matrix, has been difficult to determine because of a combination of neutral protease activities in this tissue and the intrinsic high charge density of the molecule. In this study, bovine dentin phosphophoryn (BDPP) was isolated by a proc...

journal_title：Biochemistry

authors： Stetler-Stevenson WG,Veis A

更新日期：1983-08-30 00:00:00

abstract：:The solution nuclear magnetic resonance (NMR) structures and backbone (15)N dynamics of the specialized acyl carrier protein (ACP), RpAcpXL, from Rhodopseudomonas palustris, in both the apo form and holo form modified by covalent attachment of 4'-phosphopantetheine at S37, are virtually identical, monomeric, and corre...

journal_title：Biochemistry

authors： Ramelot TA,Rossi P,Forouhar F,Lee HW,Yang Y,Ni S,Unser S,Lew S,Seetharaman J,Xiao R,Acton TB,Everett JK,Prestegard JH,Hunt JF,Montelione GT,Kennedy MA

更新日期：2012-09-18 00:00:00

abstract：:The signaling apparatus that controls bacterial chemotaxis is composed of a core complex containing chemoreceptors, the histidine autokinase CheA, and the coupling protein CheW. Site-specific spin labeling and pulsed dipolar ESR spectroscopy (PDS) have been applied to investigate the structure of a soluble ternary com...

journal_title：Biochemistry

authors： Bhatnagar J,Borbat PP,Pollard AM,Bilwes AM,Freed JH,Crane BR

更新日期：2010-05-11 00:00:00

abstract：:The effects of several guanine nucleotide analogues on (Na+,K+)-ATPase activity of membranes isolated from several tissues were analyzed to determine if a G-protein might be involved in the hormonal regulation of the (Na+,K+)-ATPase. Submillimolar concentrations of GTP gamma S, but not GMPPNP, inhibit rat skeletal mus...

journal_title：Biochemistry

authors： McGill DL

更新日期：1991-07-09 00:00:00

abstract：:The epidermal growth factor receptor (EGFR) is a transmembrane protein involved in cell signaling processes, and dysregulation of its activity often drives tumor growth. EGFR is a clinically validated tumor marker and target for antibodies and tyrosine kinase inhibitors. We demonstrate that a fusion protein of the nat...

journal_title：Biochemistry

authors： Feiner RC,Pennè I,Müller B,Müller KM

更新日期：2019-02-26 00:00:00

abstract：:Central to the pathology of Alzheimer's disease (AD) is the profuse accumulation of amyloid-beta (Abeta) peptides in the brain of affected individuals, and several amyloid precursor protein (APP) transgenic (Tg) mice models have been created to mimic Abeta deposition. Among these, the PDAPP Tg mice carrying the famili...

journal_title：Biochemistry

authors： Esh C,Patton L,Kalback W,Kokjohn TA,Lopez J,Brune D,Newell AJ,Beach T,Schenk D,Games D,Paul S,Bales K,Ghetti B,Castaño EM,Roher AE

更新日期：2005-10-25 00:00:00

abstract：:Oligodeoxynucleotides (ODNs) were conjugated to chlorambucil and used as affinity labeling reagents to study joint molecule formation by the Escherichia coli recombinase recA. Chlorambucil is a bifunctional nitrogen mustard which alkylates the N-7 position of guanine in the major groove of double-stranded DNA (dsDNA)....

journal_title：Biochemistry

authors： Podyminogin MA,Meyer RB,Gamper HB

更新日期：1995-10-10 00:00:00

abstract：:Escherichia coli 3-methyladenine DNA glycosylase II (AlkA), an adaptive response glycosylase with a broad substrate range, initiates base excision repair by flipping a lesion out of the DNA duplex and hydrolyzing the N-glycosidic bond. We used transient and steady state kinetics to determine the minimal mechanism for ...

journal_title：Biochemistry

authors： Taylor EL,O'Brien PJ

更新日期：2015-01-27 00:00:00

abstract：:Decarboxylation reactions from comparable thiamin diphosphate- and thiamin-derived adducts of p-(halomethyl)benzoylformic acids in enzymic and non-enzymic reactions, respectively, reveal critical distinctions in otherwise similar Breslow intermediates. The ratio of protonation to chloride elimination from the Breslow ...

journal_title：Biochemistry

authors： Bielecki M,Howe GW,Kluger R

更新日期：2019-08-27 00:00:00

abstract：:We have measured T1 and T2 of protein and water protons in hemoglobin solutions using broad-line pulse techniques; selective excitation and detection methods enabled the intrinsic protein and water relaxation rates, as well as the spin-transfer rate between them, to be obtained at 5, 10, and 20 MHz. Water and protein ...

journal_title：Biochemistry

authors： Eisenstadt M

更新日期：1985-07-02 00:00:00

abstract：:The inhibition mechanism of serpins requires a change in structure to entrap the target proteinase as a stable acyl-enzyme complex. Although it has generally been assumed that reactive center loop insertion and associated conformational change proceeds in a concerted manner, this has not been demonstrated directly. Th...

journal_title：Biochemistry

authors： Blouse GE,Perron MJ,Thompson JH,Day DE,Link CA,Shore JD

更新日期：2002-10-08 00:00:00

abstract：:Plasminogen is known to undergo an extremely large conformational change when it binds ligands; the two well-established conformations are either closed (absence of external ligand) or open (presence of external ligand). We show here that plasminogen is more complicated than can be accommodated by a two-state, closed/...

journal_title：Biochemistry

authors： Kornblatt JA,Schuck P

更新日期：2005-10-04 00:00:00

abstract：:A combination of mutagenesis, calorimetry, kinetics, and small-angle X-ray scattering (SAXS) has been used to study the mechanism of ligand binding energy propagation through human cytochrome P450 reductase (CPR). Remarkably, the energetics of 2',5'-ADP binding to R597 at the FAD-binding domain are affected by mutatio...

journal_title：Biochemistry

authors： Grunau A,Geraki K,Grossmann JG,Gutierrez A

更新日期：2007-07-17 00:00:00

abstract：:A general protocol for preparation of oligonucleotides containing intrastrand cross-links between the exocyclic amino groups of adjacent deoxyguanosines has been developed. A series of 2, 3, and 4 methylene cross-links was incorporated site-specifically into an 11-mer (5'-GGCAGGTGGTG-3', cross-linked positions are und...

journal_title：Biochemistry

authors： Kowalczyk A,Carmical JR,Zou Y,Van Houten B,Lloyd RS,Harris CM,Harris TM

更新日期：2002-03-05 00:00:00

abstract：:Fas and its ligand (FasL) are members of the tumor necrosis factor receptor (TNFR) and tumor necrosis factor (TNF) superfamilies, respectively. Fas-FasL interactions trigger controlled cell death (apoptosis) in the immune system and thus play a key role in the regulation of immune responses. Structural details of the ...

journal_title：Biochemistry

authors： Starling GC,Kiener PA,Aruffo A,Bajorath J

更新日期：1998-03-17 00:00:00

abstract：:We have studied the tridecadeoxynucleotide CGCGAATTACGCG (I), which contains an additional A at position 9 compared to the dodecanucleotide of which the crystal structure has been determined. Sequence I exhibits no distinct melting curve and also has a concentration-dependent pattern of peaks on reverse-phase chromato...

journal_title：Biochemistry

authors： Roy S,Weinstein S,Borah B,Nickol J,Appella E,Sussman JL,Miller M,Shindo H,Cohen JS

更新日期：1986-11-18 00:00:00

abstract：:A covalent complex between tRNA (m5U54)methyltransferase, 5-fluorouridine tRNA(Phe), and S-adenosyl-L-[methyl-3H]methionine was formed in vitro and purified. Previously, it was shown that in this complex the 6-position of fluorouridine-54 is covalently linked to a catalytic nucleophile and the 5-position is bound to t...

journal_title：Biochemistry

authors： Kealey JT,Santi DV

更新日期：1991-10-08 00:00:00

abstract：:Phosphorylated proteins may play an important role in regulating the metabolism or function of rod photoreceptors. In mammalian retinas, a photoreceptor protein of 33 000 (33K) molecular weight is phosphorylated in a cyclic nucleotide dependent manner in vitro. Since light initiates the activation of a photoreceptor-s...

journal_title：Biochemistry

authors： Lee RH,Brown BM,Lolley RN

更新日期：1984-04-24 00:00:00

abstract：:To contribute to the understanding of membrane protein function upon application of pressure as relevant for understanding, for example, the physiology of deep sea organisms or for baroenzymological biotechnical processes, we investigated the influence of hydrostatic pressure on the activity of Na+,K+-ATPase enriched ...

journal_title：Biochemistry

authors： Powalska E,Janosch S,Kinne-Saffran E,Kinne RK,Fontes CF,Mignaco JA,Winter R

更新日期：2007-02-13 00:00:00

abstract：:When grown under O2-limited conditions, Escherichia coli expresses a cytochrome bd quinol oxidase that has an unusually high affinity for O2. We have studied the reaction of cytochrome bd with CO and O2 by rapid-reaction spectrophotometry. The reduced enzyme forms a photosensitive ferrocytochrome d-CO complex, and fol...

journal_title：Biochemistry

authors： Hill BC,Hill JJ,Gennis RB

更新日期：1994-12-20 00:00:00

abstract：:The UDP-glucuronosyltransferase (UGT) isozyme system is critical for protecting the body against endogenous and exogenous chemicals by linking glucuronic acid donated by UDP-glucuronic acid to a lipophilic acceptor substrate. UGTs convert metabolites, dietary constituents, and environmental toxicants to highly excreta...

journal_title：Biochemistry

authors： Banerjee R,Pennington MW,Garza A,Owens IS

更新日期：2008-07-15 00:00:00

abstract：:Using our Escherichia coli expression system, we have produced five mutant recombinant (r) hemoglobins (Hbs): r Hb (alpha V96 W), r Hb Presbyterian (beta N108K), r Hb Yoshizuka (beta N108D), r Hb (alpha V96W, beta N108K), and r Hb (alpha V96W, beta N108D). These r Hbs allow us to investigate the effect on the structur...

journal_title：Biochemistry

authors： Tsai CH,Shen TJ,Ho NT,Ho C

更新日期：1999-07-06 00:00:00

abstract：:Polyhistidine affinity tags are routinely employed as a convenient means of purifying recombinantly expressed proteins. A tacit assumption is commonly made that His tags have little influence on protein structure and function. Attachment of a His tag to the N-terminus of the robust globular protein myoglobin leads to ...

journal_title：Biochemistry

authors： Thielges MC,Chung JK,Axup JY,Fayer MD

更新日期：2011-06-28 00:00:00

abstract：:The ribonucleoprotein ribonuclease P (RNase P) cleaves all tRNA precursors endonucleolitically to produce the mature 5'-end. Dictyostelium discoideum RNase P displays an absolute requirement for Mg2+. Only the alkaline earth cations Ca2+, Sr2+, and Ba2+, under appropriate conditions can substitute to some extent for M...

journal_title：Biochemistry

authors： Tekos A,Stathopoulos C,Drainas D

更新日期：1998-11-03 00:00:00

abstract：:Notwithstanding the relevance of their biological function, slow motions in proteins, beyond the microsecond range, are still poorly understood and often elusive. We propose that acrylamide quenching of Trp phosphorescence of deeply buried residues, when extended over the entire accessible range of lifetime measuremen...

journal_title：Biochemistry

authors： Strambini GB,Gonnelli M

更新日期：2009-08-11 00:00:00

abstract：:6-Hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK) catalyzes the pyrophosphoryl transfer from ATP to 6-hydroxymethyl-7,8-dihydropterin (HP), the first reaction in the folate biosynthetic pathway. Arginine residues 82 and 92, strictly conserved in 35 HPPK sequences, play dynamic roles in the catalytic cycle of ...

journal_title：Biochemistry

authors： Blaszczyk J,Li Y,Shi G,Yan H,Ji X

更新日期：2003-02-18 00:00:00

abstract：:Thromboxane A(2) receptor (TP receptor), a prostanoid receptor, belongs to the G protein-coupled receptor family, composed of three intracellular loops and three extracellular loops connecting seven transmembrane helices. The highly conserved extracellular domains of the prostanoid receptors were found in the second e...

journal_title：Biochemistry

authors： Ruan KH,So SP,Wu J,Li D,Huang A,Kung J

更新日期：2001-01-09 00:00:00

abstract：:The Vmax/Km (microM -1 min -1.) for bovine factor X activation by bovine factor IXa alpha, in the presence of sufficient [Ca2+] to saturate the initial reaction rate, was 0.007. When factor IXa beta was substituted for factor IXa alpha in this reaction, the Vmax/Km decreased to 0.001, suggesting that factor IXa alpha ...

journal_title：Biochemistry

authors： Link RP,Castellino FJ

更新日期：1983-08-16 00:00:00

abstract：:Phosphodeoxyribomutase from Escherichia coli has been purified to homogeneity. Chromatography on DEAE-Sephadex revealed three peaks of enzyme activity, designated form I, form II, and form III. Form III could be further separated into form III-1 and form III-2 by polyacrylamide gel electrophoresis. The four different ...

journal_title：Biochemistry

authors： Leer JC,Hammer-Jespersen K

更新日期：1975-02-11 00:00:00

abstract：:Lecithin retinol acyltransferase (LRAT) catalyzes the esterification of all-trans-retinol into all-trans-retinyl ester, an essential reaction in the vertebrate visual cycle. Since all-trans-retinyl esters are the substrates for the isomerization reaction that generates 11-cis-retinoids, this esterification reaction is...

journal_title：Biochemistry

authors： Bok D,Ruiz A,Yaron O,Jahng WJ,Ray A,Xue L,Rando RR

更新日期：2003-05-27 00:00:00