Effects of substitutions of lysine and aspartic acid for asparagine at beta 108 and of tryptophan for valine at alpha 96 on the structural and functional properties of human normal adult hemoglobin: roles of alpha 1 beta 1 and alpha 1 beta 2 subunit inter

abstract：:The manganese transport regulator (MntR) of Bacillus subtilis is activated by Mn(2+) to repress transcription of genes encoding transporters involved in the uptake of manganese. MntR is also strongly activated by cadmium, both in vivo and in vitro, but it is poorly activated by other metal cations, including calcium a...

journal_title：Biochemistry

authors： Kliegman JI,Griner SL,Helmann JD,Brennan RG,Glasfeld A

更新日期：2006-03-21 00:00:00

abstract：:Previous work from this laboratory has established that the NADPH-menadione oxidoreductase reaction catalyzed by methylenetetrahydrofolate reductase from pig liver proceeds by Ping Pong Bi Bi kinetics and that the reductive half-reaction is rate limiting in steady-state turnover. We have now shown that methylenetetrah...

journal_title：Biochemistry

authors： Vanoni MA,Matthews RG

更新日期：1984-10-23 00:00:00

abstract：:We have expressed the alpha and beta subunits of bacterial luciferase, encoded by luxA and luxB, from separate plasmids in Escherichia coli and developed an efficient purification scheme that yields many milligrams of protein of greater than 90% homogeneity. Earlier experiments showed that subunits synthesized separat...

journal_title：Biochemistry

authors： Sinclair JF,Waddle JJ,Waddill EF,Baldwin TO

更新日期：1993-05-18 00:00:00

abstract：:Light-induced hypsochromic shifts of the Q(y) absorption band of the bacteriochlorophyll dimer (P) from 865 to 850 nm were identified using continuous illumination of dark-adapted reaction centers (RCs) from Rhodobacter capsulatus when dispersed in the most commonly used detergent, the zwitterionic lauryl N-dimethylam...

journal_title：Biochemistry

authors： Deshmukh SS,Akhavein H,Williams JC,Allen JP,Kalman L

更新日期：2011-06-14 00:00:00

abstract：:Excitation energy transfer studies were carried out to determine the distance between the adenosinetriphosphatase (ATPase) site and a unique "fast-reacting" sulfhydryl (referred to as SH1) in myosin subfragment 1. The fluorescent moiety of the probe N-(iodoacetyl)-N'-(5-sulfo-1-naphthyl)ethylene-diamine was used as th...

journal_title：Biochemistry

authors： Tao T,Lamkin M

更新日期：1981-08-18 00:00:00

abstract：:Fluorescence recovery after photobleaching was used to measure the diffusion coefficient of green fluorescent protein (GFP, 27 kDa) in Escherichia coli in the presence or absence of four coexpressed proteins: cytoplasmic maltose binding protein (42 kDa), tau-40 (45 kDa), alpha-synuclein (14 kDa), or calmodulin (17 kDa...

journal_title：Biochemistry

authors： Slade KM,Baker R,Chua M,Thompson NL,Pielak GJ

更新日期：2009-06-16 00:00:00

abstract：:The mechanism of herpesviral protease activation upon dimerization was studied using two independent spectroscopic assays augmented by directed mutagenesis. Spectroscopic changes, attributable to dimer interface conformational plasticity, were observed upon dimerization of Kaposi's sarcoma-associated herpesvirus prote...

journal_title：Biochemistry

authors： Pray TR,Reiling KK,Demirjian BG,Craik CS

更新日期：2002-02-05 00:00:00

abstract：:We have systematically validated the activity and inhibition of a HIV-1 protease (PR) variant bearing 17 mutations (PR(S17)), selected to represent high resistance by machine learning on genotype-phenotype data. Three of five mutations in PR(S17) correlating with major drug resistance, M46L, G48V, and V82S, and five o...

journal_title：Biochemistry

authors： Park JH,Sayer JM,Aniana A,Yu X,Weber IT,Harrison RW,Louis JM

更新日期：2016-04-26 00:00:00

abstract：:Stability increments of terminal unpaired nucleotides (dangling ends) and terminal base pairs on the core helixes AUGCAU and UGCGCA are reported. Enthalpy, entropy, and free energy changes of helix formation were measured spectrophotometrically for 18 oligoribonucleotides containing the core sequences. The results ind...

journal_title：Biochemistry

authors： Sugimoto N,Kierzek R,Turner DH

更新日期：1987-07-14 00:00:00

abstract：:The structure of the adduct formed by reaction of acetaldehyde and Met5-enkephalin has been determined by analysis of 400-MHz proton spectra: two-dimensional J spectroscopy was used to resolve and measure virtually all the overlapping resonances, and decoupling difference spectroscopy was used to assign the resonances...

journal_title：Biochemistry

authors： Gidley MJ,Hall LD,Sanders JK,Summers MC

更新日期：1981-06-23 00:00:00

abstract：:The Stk1/Stp1 and GraSR signal-transduction pathways are two distinct pathways in Staphylococcus aureus that rely on a reversible phosphorylation process in transducing external stimuli intracellularly. Stk1/Stp1 is an eukaryote-like Ser/Thr kinase phosphatase pair involved in purine biosynthesis, cell-wall metabolism...

journal_title：Biochemistry

authors： Fridman M,Williams GD,Muzamal U,Hunter H,Siu KW,Golemi-Kotra D

更新日期：2013-11-12 00:00:00

abstract：:Catalase HPII from Escherichia coli is a homotetramer of 753 residue subunits. The multimer displays a number of unusual structural features, including interwoven subunits and a covalent bond between Tyr415 and His392, that would contribute to its rigidity and stability. As the temperature of a solution of HPII in 50 ...

journal_title：Biochemistry

authors： Switala J,O'Neil JO,Loewen PC

更新日期：1999-03-30 00:00:00

abstract：:The Pf1 gene 5 protein forms a large helical nucleoprotein complex (Mr = 3.1 x 10(7)) with single-stranded viral DNA, from which a 32 amino acid sequence rich in alanine, proline, and glutamine residues can be removed from the C-terminus by limited proteolysis. Sharp resonances in the 1H NMR spectrum of the Pf1 nucleo...

journal_title：Biochemistry

authors： Davis KG,Plyte SE,Robertson SR,Cooper A,Kneale GG

更新日期：1995-01-10 00:00:00

abstract：:The full three-dimensional structure of the catalytic domain of human stromelysin-1 (SCD) complexed to a novel and potent, nonpeptidic inhibitor has been determined by nuclear magnetic resonance spectroscopy (NMR). To accurately mimic assay conditions, the structure was obtained in Tris buffer at pH 6.8 and without th...

journal_title：Biochemistry

authors： Li YC,Zhang X,Melton R,Ganu V,Gonnella NC

更新日期：1998-10-06 00:00:00

abstract：:Botulinum neurotoxins (BoNTs) elicit flaccid paralysis through cleavage of SNARE proteins within peripheral neurons. There are seven serotypes of the BoNTs, termed A-G, which differ in the SNARE protein and/or site that is cleaved. BoNTs are single-chain toxins that comprise an N-terminal zinc metalloprotease domain t...

journal_title：Biochemistry

authors： Baldwin MR,Barbieri JT

更新日期：2007-03-20 00:00:00

abstract：:The σ1 receptor is an integral membrane protein that shares no homology with other receptor systems, has no unequivocally identified natural ligands, but appears to play critical roles in a wide variety of cell functions. While the number of reports of the possible functions of the σ1 receptor is increasing, almost no...

journal_title：Biochemistry

authors： Brune S,Schepmann D,Klempnauer KH,Marson D,Dal Col V,Laurini E,Fermeglia M,Wünsch B,Pricl S

更新日期：2014-05-13 00:00:00

abstract：:To develop molecules capable of directly probing the catechol binding region of the beta(2)-adrenergic receptor (beta(2)AR), novel benzophenone- and fluorenone-based beta(2)AR antagonists were prepared as potential photoaffinity probes. While the benzophenone-containing ligands bound with relatively modest affinity, o...

journal_title：Biochemistry

authors： Wu Z,Ruoho AE

更新日期：2000-10-24 00:00:00

abstract：:Z-disc-associated, alternatively spliced, PDZ motif-containing protein (ZASP) is a principal component of the sarcomere. The three prevalent isoforms of ZASP in skeletal muscle are generated by alternative splicing of exons 9 and 10. The long isoforms, either having (ZASP-L) or lacking exon 10 (ZASP-LΔex10), include a...

journal_title：Biochemistry

authors： Watts NR,Zhuang X,Kaufman JD,Palmer IW,Dearborn AD,Coscia S,Blech-Hermoni Y,Alfano C,Pastore A,Mankodi A,Wingfield PT

更新日期：2017-04-11 00:00:00

abstract：:Replication protein A (RPA) is a heterotrimeric protein that is required for DNA replication and most DNA repair pathways. RPA has previously been shown to play a role in recognizing and binding damaged DNA during nucleotide excision repair (NER). RPA has also been suggested to play a role in psoralen DNA interstrand ...

journal_title：Biochemistry

authors： Patrick SM,Tillison K,Horn JM

更新日期：2008-09-23 00:00:00

abstract：:Escherichia coli (A)BC excinuclease is the major enzyme responsible for removing bulky adducts, such as pyrimidine dimers and 6-4 photoproducts, from DNA. Mutants deficient in this enzyme are extremely sensitive to UV and UV-mimetic agents, but not to oxidizing agents, or ionizing radiation which damages DNA in part b...

journal_title：Biochemistry

authors： Lin JJ,Sancar A

更新日期：1989-10-03 00:00:00

abstract：:Chemosensory cilia of olfactory receptor neurons contain an adenylate cyclase which is stimulated by high concentrations of odorants. Cyclic AMP produced by this enzyme has been proposed to act as second messenger in olfactory transduction. Here we report that olfactory cilia contain calmodulin and that calmodulin pot...

journal_title：Biochemistry

authors： Anholt RR,Rivers AM

更新日期：1990-05-01 00:00:00

abstract：:Synthetic O-glycopeptides containing one or two GalNAc residues attached to Ser or Thr were used as substrates to investigate the effect of peptide structure on the activity of crude preparations of UDP-Gal:GalNAc alpha-R beta 3-Gal-transferase from pig stomach and pig and rat colonic mucosa and of a partially purifie...

journal_title：Biochemistry

authors： Brockhausen I,Möller G,Merz G,Adermann K,Paulsen H

更新日期：1990-11-06 00:00:00

abstract：:The 24-kDa subunit of mitochondrial NADH-ubiquinone reductase (complex I) is an iron-sulfur protein that is present in the flavoprotein or NADH dehydrogenase II subcomplex. It is a nuclear gene product and is imported into the organelle. A group of human patients with mitochondrial myopathy have been shown to have red...

journal_title：Biochemistry

authors： Pilkington SJ,Walker JE

更新日期：1989-04-18 00:00:00

abstract：:We investigated how the intrinsic sequence-dependent properties probed via the phosphate linkages (BI ↔ BII equilibrium) influence the preferred shape of free DNA, and how this affects the nucleosome formation. First, this exploits NMR solution studies of four B-DNA dodecamers that together cover 39 base pairs of the ...

journal_title：Biochemistry

authors： Xu X,Ben Imeddourene A,Zargarian L,Foloppe N,Mauffret O,Hartmann B

更新日期：2014-09-09 00:00:00

abstract：:The structure of PsbQ, one of the three main extrinsic proteins associated with the oxygen-evolving complex (OEC) of higher plants and green algae, is examined by Fourier transform infrared (FTIR) and circular dichroic (CD) spectroscopy and by computational structural prediction methods. This protein, together with tw...

journal_title：Biochemistry

authors： Balsera M,Arellano JB,Gutiérrez JR,Heredia P,Revuelta JL,De Las Rivas J

更新日期：2003-02-04 00:00:00

abstract：:In the nacre or aragonite layer of the mollusk shell, proteomes that regulate both the early stages of nucleation and nano-to-mesoscale assembly of nacre tablets from mineral nanoparticle precursors exist. Several approaches have been developed to understand protein-associated mechanisms of nacre formation, yet we sti...

journal_title：Biochemistry

authors： Chang EP,Roncal-Herrero T,Morgan T,Dunn KE,Rao A,Kunitake JA,Lui S,Bilton M,Estroff LA,Kröger R,Johnson S,Cölfen H,Evans JS

更新日期：2016-04-26 00:00:00

abstract：:Biogenesis of the Gram-negative outer membrane involves the chaperone seventeen kilodalton protein (Skp). A Skp trimer is currently thought to bind its unfolded outer membrane protein (uOMP) substrates. Using sedimentation equilibrium, we discovered that Skp is not an obligate trimer under physiological conditions and...

journal_title：Biochemistry

authors： Sandlin CW,Zaccai NR,Fleming KG

更新日期：2015-12-08 00:00:00

abstract：:The dynamics of the enthalpy and volume changes found in the photodissociation of CO from sperm whale carboxymyoglobin and two site-directed mutants in which arginine-45 is replaced by glycine and asparagine are examined by photoacoustic calorimetry. An intermediate is observed whose lifetime at 20 degrees C is 700 ns...

journal_title：Biochemistry

authors： Westrick JA,Peters KS,Ropp JD,Sligar SG

更新日期：1990-07-17 00:00:00

abstract：:Saccharomyces cerevisiae tRNA and 5S RNA, Escherichia coli 5S RNA, and wheat germ 5S RNA have each been specifically spin-labeled at the 3'-terminal ribose to give morpholino-spin-labeled (MSL) RNAs. Enzymatic hydrolysis with pancreatic RNase, followed by anion-exchange chromatography, confirms the site of attachment ...

journal_title：Biochemistry

authors： Luoma GA,Herring FG,Marshall AG

更新日期：1982-12-07 00:00:00

abstract：:The steady-state and pre-steady-state kinetic parameters for the interaction of E with the fluorogenic substrate 2-aminobenzoyl-Thr-Ile-Nle-Phe(p-NO(2))-Gln-Arg-NH(2) were determined in 1.25 M NaCl, 0.1 M MES-TRIS at pH 6.0 at 25 degrees C. At low concentrations of enzyme, the values of the K(m) and k(cat) calculated ...

journal_title：Biochemistry

authors： Porter DJ,Hanlon MH,Furfine ES

更新日期：2002-01-29 00:00:00