当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Coupling factor 1 from Escherichia coli lacking subunits delta and epsilon: preparation and specific binding to depleted membranes, mediated by subunits delta or epsilon.

Coupling factor 1 from Escherichia coli lacking subunits delta and epsilon: preparation and specific binding to depleted membranes, mediated by subunits delta or epsilon.

Abstract：

:A procedure for the preparation of coupling factor 1 (F1) from Escherichia coli lacking subunits delta and epsilon is described. Using chloroform and dimethyl sulfoxide, we can isolate F1 containing only subunits alpha, beta, and gamma [F1(alpha beta gamma)] directly from membrane vesicles in 10-mg quantities. Pure and active subunits delta and epsilon were prepared from five-subunit F1 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. After addition of these subunits, F1(alpha beta gamma) is as active in reconstituting ATP-dependent transhydrogenase as five-subunit F1. The ATPase activity of F1 (alpha beta gamma) is inhibited by subunit epsilon in a 1:1 stoichiometry to the same extent (approximately equal to 90%) and with the same affinity (Ki = 0.2-0.8 nM) as reported earlier [Dunn, S.D. (1982) J. Biol. Chem. 257, 7354-7359]. In the presence of either delta or epsilon, F1(alpha beta gamma) binds to F1-depleted membrane vesicles and to liposomes containing the membrane sector (F0) of the ATP synthase to an extent commensurate with the F0 content. The binding ratios epsilon/F1 (alpha beta gamma) and probably also delta/F1 (alpha beta gamma) are close to unity. The specific, delta- or epsilon-deficient F1.F0 complexes presumably formed show ATPase activities sensitive to subunit epsilon but not to dicyclohexylcarbodiimide, and no energy-transfer capabilities. Binding studies at different pH values suggest that F1-F0 interactions in the presence of both subunits delta and epsilon are similar to a combination of those mediated by delta or epsilon alone.(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

Biochemistry

journal_title

Biochemistry

authors

Tuttas-Dörschug R,Hanstein WG

doi

10.1021/bi00438a030

subject

Has Abstract

pub_date

1989-06-13 00:00:00

pages

5107-13

issue

12

eissn

0006-2960

issn

1520-4995

journal_volume

28

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Investigation of the shape and size of myosin subfragment 1 using small-angle X-ray scattering.

    abstract：:Measurement of x-ray scattering at very small angles by solutions of myosin subfragment 1 (S1) yields a radius of gyration of 3.24 nm (mean) +/- 0.03 nm (standard error for N = 9). If S1 is assumed to be ellipsoid of revolution, of uniform electron density, with a molecular weight of 1.15 X 10(5) and a partial specifi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00605a009

    authors： Kretzschmar KM,Mendelson RA,Morales MF

    更新日期：1978-06-13 00:00:00

  • Residues 207, 216, and 221 and the catalytic activity of mGSTA1-1 and mGSTA2-2 toward benzo[a]pyrene-(7R,8S)-diol-(9S,10R)-epoxide.

    abstract：:Murine class alpha glutathione S-transferase subunit types A2 (mGSTA2-2) and A1 (mGSTA1-1) have high catalytic efficiency for glutathione (GSH) conjugation of the ultimate carcinogenic metabolite of benzo[a]pyrene, (+)-anti-7,8-dihydroxy-9,10-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene, [(+)-anti-BPDE]. Only 10 residues dif...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi026778+

    authors： Gu Y,Xiao B,Wargo HL,Bucher MH,Singh SV,Ji X

    更新日期：2003-02-04 00:00:00

  • Benzothiazepine binding domain of purified L-type calcium channels: direct labeling using a novel fluorescent diltiazem analogue.

    abstract：:We have synthesized a series of N-propylamino-substituted benzazepinones (NPSBs) as specific probes for the benzothiazepinone (BTZ) binding domain of muscle L-type calcium channels (LTCCs). NPSBs were identified which possess high affinity for the channel after purification. We synthesized a fluorescent NPSB, DMBODIPY...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00010a039

    authors： Brauns T,Cai ZW,Kimball SD,Kang KC,Haugland RP,Berger W,Berjukov S,Hering S,Glossmann H,Striessnig J

    更新日期：1995-03-14 00:00:00

  • Dissecting the electrostatic interactions and pH-dependent activity of a family 11 glycosidase.

    abstract：:Previous studies of the low molecular mass family 11 xylanase from Bacillus circulans show that the ionization state of the nucleophile (Glu78, pK(a) 4.6) and the acid/base catalyst (Glu172, pK(a) 6.7) gives rise to its pH-dependent activity profile. Inspection of the crystal structure of BCX reveals that Glu78 and Gl...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：

    authors： Joshi MD,Sidhu G,Nielsen JE,Brayer GD,Withers SG,McIntosh LP

    更新日期：2001-08-28 00:00:00

  • Structural insight into how Streptomyces coelicolor maltosyl transferase GlgE binds α-maltose 1-phosphate and forms a maltosyl-enzyme intermediate.

    abstract：:GlgE (EC 2.4.99.16) is an α-maltose 1-phosphate:(1→4)-α-d-glucan 4-α-d-maltosyltransferase of the CAZy glycoside hydrolase 13_3 family. It is the defining enzyme of a bacterial α-glucan biosynthetic pathway and is a genetically validated anti-tuberculosis target. It catalyzes the α-retaining transfer of maltosyl units...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi500183c

    authors： Syson K,Stevenson CE,Rashid AM,Saalbach G,Tang M,Tuukkanen A,Svergun DI,Withers SG,Lawson DM,Bornemann S

    更新日期：2014-04-22 00:00:00

  • Behavior of fragmented calcium (II) adenosine triphosphatase from sarcoplasmic reticulum in detergent solution.

    abstract：:The behavior of Ca2+-ATPase from sarcoplasmic reticulum in detergent solution was compared with that of Ca2+-ATPase which had been cleaved in half by limited trypsin digestion. Attempts to dissociate the fragments (I and II) with an excess of detergent micelles demonstrated that fragments I and II are structurally dep...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00612a028

    authors： Rizzolo LJ,Tanford C

    更新日期：1978-09-19 00:00:00

  • Peptide binding by a fragment of calmodulin composed of EF-hands 2 and 3.

    abstract：:Calmodulin (CaM) is composed of two EF-hand domains tethered by a flexible linker. Upon Ca2+-binding, a fragment of CaM encompassing EF-hands 2 and 3 (CaM2/3; residues 46-113) folds into a structure remarkably similar to the N- and C-domains of CaM. In this study, we demonstrate that Ca2+-ligated CaM2/3 can also bind ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi700265j

    authors： Lakowski TM,Lee GM,Lelj-Garolla B,Okon M,Reid RE,McIntosh LP

    更新日期：2007-07-24 00:00:00

  • Characterization of human aspartoacylase: the brain enzyme responsible for Canavan disease.

    abstract：:Aspartoacylase catalyzes the deacetylation of N-acetylaspartic acid (NAA) to produce acetate and L-aspartate and is the only brain enzyme that has been shown to effectively metabolize NAA. Although the exact role of this enzymatic reaction has not yet been completely elucidated, the metabolism of NAA appears to be nec...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi052608w

    authors： Le Coq J,An HJ,Lebrilla C,Viola RE

    更新日期：2006-05-09 00:00:00

  • Low-light-induced formation of semicrystalline photosystem II arrays in higher plant chloroplasts.

    abstract：:Remodeling of photosynthetic machinery induced by growing spinach plants under low light intensities reveals an up-regulation of light-harvesting complexes and down-regulation of photosystem II and cytochrome b6f complexes in intact thylakoids and isolated grana membranes. The antenna size of PSII increased by 40-60% ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi700748y

    authors： Kirchhoff H,Haase W,Wegner S,Danielsson R,Ackermann R,Albertsson PA

    更新日期：2007-10-02 00:00:00

  • Solution structure of the N-terminal EGF-like domain from human factor VII.

    abstract：:Blood coagulation is initiated by Ca(2+)-dependent binding of coagulation factor VIIa (FVIIa) to its cofactor, tissue factor (TF). The TF:FVIIa complex activates factors IX and X, ultimately leading to the formation of thrombin and the coagulation of blood. FVII consists of an N-terminal gamma-carboxyglutamic-acid-con...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi980522f

    authors： Muranyi A,Finn BE,Gippert GP,Forsén S,Stenflo J,Drakenberg T

    更新日期：1998-07-28 00:00:00

  • Resolution and activity of adenylate cyclase components in a zwitterionic cholate derivative [3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate].

    abstract：:Bovine brain adenylate cyclase was solubilized with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), sodium cholate, sodium deoxycholate, or these detergents plus (NH4)2SO4. The specific activity of the extract obtained with 13 mM CHAPS alone was several times those of the other detergent extracts wi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00258a019

    authors： Bitonti AJ,Moss J,Hjelmeland L,Vaughan M

    更新日期：1982-07-20 00:00:00

  • Cellular Concentrations of Nucleotide Diphosphate-Chelated Magnesium Ions Accelerate Catalysis by RNA and DNA Enzymes.

    abstract：:RNAs are involved in myriad cellular events. In general, RNA function is affected by cellular conditions. For instance, molecular crowding promotes RNA folding through compaction of the RNA. Metabolites generally destabilize RNA secondary structure, which improves RNA folding cooperativity and increases the fraction o...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.9b00578

    authors： Yamagami R,Huang R,Bevilacqua PC

    更新日期：2019-09-24 00:00:00

  • Specific effects of chloride on the photocycle of E194Q and E204Q mutants of bacteriorhodopsin as measured by FTIR spectroscopy.

    abstract：:Low-temperature Fourier transform infrared spectroscopy has been used to study mutants of Glu194 and Glu204, two amino acids that are involved in proton release to the extracellular side of bacteriorhodopsin. Difference spectra of films of E194Q, E204Q, E194Q/E204Q, E9Q/E194Q/E204Q, and E9Q/E74Q/E194Q/E204Q at 243, 27...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi025654u

    authors： Lazarova T,Sanz C,Sepulcre F,Querol E,Padrós E

    更新日期：2002-06-25 00:00:00

  • Crystal structure of Baeyer-Villiger monooxygenase MtmOIV, the key enzyme of the mithramycin biosynthetic pathway .

    abstract：:Baeyer-Villiger monooxygenases (BVMOs), mostly flavoproteins, were shown to be powerful biocatalysts for synthetic organic chemistry applications and were also suggested to play key roles for the biosyntheses of various natural products. Here we present the three-dimensional structure of MtmOIV, a 56 kDa homodimeric F...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi8023509

    authors： Beam MP,Bosserman MA,Noinaj N,Wehenkel M,Rohr J

    更新日期：2009-06-02 00:00:00

  • Structural changes in cytochrome c upon hydrogen-deuterium exchange.

    abstract：:The resonance Raman spectra of yeast ferri- and ferro-iso-1-cytochrome c dissolved in H2O and D2O are reported. Hydrogen exchange in the protein leads to distinct spectral changes of heme vibrational bands, particularly in the region between 670 and 710 cm-1 and at approximately 443 and approximately 450 cm-1. The lat...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00214a013

    authors： Hildebrandt P,Vanhecke F,Heibel G,Mauk AG

    更新日期：1993-12-28 00:00:00

  • The 14-3-3 protein affects the conformation of the regulatory domain of human tyrosine hydroxylase.

    abstract：:Tyrosine hydroxylase (TH) catalyzes the first step in the biosynthesis of catecholamines. Regulation of TH enzyme activity is controlled through the posttranslational modification of its regulatory domain. The regulatory domain of TH can be phosphorylated at four serines (8, 19, 31, and 40) by a variety of protein kin...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi7019468

    authors： Obsilova V,Nedbalkova E,Silhan J,Boura E,Herman P,Vecer J,Sulc M,Teisinger J,Dyda F,Obsil T

    更新日期：2008-02-12 00:00:00

  • Mapping of factor XIII solvent accessibility as a function of activation state using chemical modification methods.

    abstract：:The transglutaminase Factor XIII (FXIII) catalyzes the formation of covalent cross-links between adjacent noncovalently associated fibrin chains in blood coagulation. The resulting covalently cross-linked hard clot is much more mechanically stable and resistant to proteolytic degradation. FXIII is activated by the ser...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi049260+

    authors： Turner BT Jr,Sabo TM,Wilding D,Maurer MC

    更新日期：2004-08-03 00:00:00

  • Solution structure and lipid membrane partitioning of VSTx1, an inhibitor of the KvAP potassium channel.

    abstract：:VSTx1 is a voltage sensor toxin from the spider Grammostola spatulata that inhibits KvAP, an archeabacterial voltage-activated K(+) channel whose X-ray structure has been reported. Although the receptor for VSTx1 and the mechanism of inhibition are unknown, the sequence of the toxin is related to hanatoxin (HaTx) and ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0477034

    authors： Jung HJ,Lee JY,Kim SH,Eu YJ,Shin SY,Milescu M,Swartz KJ,Kim JI

    更新日期：2005-04-26 00:00:00

  • Mechanism of interactions of alpha-naphthoflavone with cytochrome P450 3A4 explored with an engineered enzyme bearing a fluorescent probe.

    abstract：:Design of a partially cysteine-depleted C98S/C239S/C377S/C468A cytochrome P450 3A4 mutant designated CYP3A4(C58,C64) allowed site-directed incorporation of thiol-reactive fluorescent probes into alpha-helix A. The site of modification was identified as Cys-64 with the help of CYP3A4(C58) and CYP3A4(C64), each bearing ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi061944p

    authors： Tsalkova TN,Davydova NY,Halpert JR,Davydov DR

    更新日期：2007-01-09 00:00:00

  • Mechanism of the calcium-induced trans-cis isomerization of a non-prolyl peptide bond in Clostridium histolyticum collagenase.

    abstract：:cis peptide bonds in proteins are often rate-limiting steps in protein folding or conformational change and are frequently stabilized by metal ions. In the collagen-binding domain of Clostridium histolyticum collagenase, the binding of calcium ions triggers the formation of a cis peptide bond. We present free energy s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi1007047

    authors： Spiriti J,van der Vaart A

    更新日期：2010-06-29 00:00:00

  • Characterization of electron tunneling and hole hopping reactions between different forms of MauG and methylamine dehydrogenase within a natural protein complex.

    abstract：:Respiration, photosynthesis, and metabolism require the transfer of electrons through and between proteins over relatively long distances. It is critical that this electron transfer (ET) occur with specificity to avoid cellular damage, and at a rate that is sufficient to support the biological activity. A multistep ho...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi300817d

    authors： Choi M,Shin S,Davidson VL

    更新日期：2012-09-04 00:00:00

  • CoCl(2) induces apoptosis via the 18 kDa translocator protein in U118MG human glioblastoma cells.

    abstract：:The 18 kDa translocator protein (TSPO), formerly known as the peripheral-type benzodiazepine receptor, has been reported to be closely associated with the mitochondrial permeability transition pore (MPTP). TSPO is believed to exert pro-apoptotic functions via modulation of MPTP opening. Cobalt chloride (CoCl(2)), whic...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi900064t

    authors： Zeno S,Zaaroor M,Leschiner S,Veenman L,Gavish M

    更新日期：2009-06-02 00:00:00

  • Studies of synthetic peptides of human apolipoprotein A-I containing tandem amphipathic alpha-helixes.

    abstract：:In mature human apolipoprotein A-I (apo A-I), the amino acid residues 1-43 are encoded by exon 3, whereas residues 44-243 are encoded by exon 4 of the apo A-I gene. The region encoded by exon 4 of the apo A-I gene contains 10 tandem amphipathic alpha-helixes; their location and the class to which they belong are as fo...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi980042o

    authors： Mishra VK,Palgunachari MN,Datta G,Phillips MC,Lund-Katz S,Adeyeye SO,Segrest JP,Anantharamaiah GM

    更新日期：1998-07-14 00:00:00

  • Structure and inhibition of a quorum sensing target from Streptococcus pneumoniae.

    abstract：:Streptococcus pneumoniae 5'-methylthioadenosine/S-adenosylhomocysteine hydrolase (MTAN) catalyzes the hydrolytic deadenylation of its substrates to form adenine and 5-methylthioribose or S-ribosylhomocysteine (SRH). MTAN is not found in mammals but is involved in bacterial quorum sensing. MTAN gene disruption affects ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi061184i

    authors： Singh V,Shi W,Almo SC,Evans GB,Furneaux RH,Tyler PC,Painter GF,Lenz DH,Mee S,Zheng R,Schramm VL

    更新日期：2006-10-31 00:00:00

  • A sequential binding mechanism in a PDZ domain.

    abstract：:Conformational selection and induced fit are two well-known mechanisms of allosteric protein-ligand interaction. Some proteins, like ubiquitin, have recently been found to exist in multiple conformations at equilibrium, suggesting that the conformational selection may be a general mechanism of interaction, in particul...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi900559k

    authors： Chi CN,Bach A,Engström A,Wang H,Strømgaard K,Gianni S,Jemth P

    更新日期：2009-08-04 00:00:00

  • Simple method for selecting catalytic monoclonal antibodies that exhibit turnover and specificity.

    abstract：:Monoclonal antibodies were raised against a mono-p-nitrophenyl phosphonate ester to elicit catalytic antibodies capable of hydrolyzing the analogous p-nitrophenyl ester or carbonate. Potential catalytic antibody producing clones were selected, by use of a competitive inhibition assay, on the basis of their affinity fo...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00494a023

    authors： Tawfik DS,Zemel RR,Arad-Yellin R,Green BS,Eshhar Z

    更新日期：1990-10-23 00:00:00

  • Groups with polar characteristics can locate at both shallow and deep locations in membranes: the behavior of dansyl and related probes.

    abstract：:To understand the relationship between the chemical structure of polar molecules and their membrane location, the behavior of dansyl (dimethylaminonaphthalenesulfonyl) and related polar fluorescent probes was examined. The depth of these probes in lipid bilayers was determined by parallax analysis of fluorescence quen...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9726234

    authors： Asuncion-Punzalan E,Kachel K,London E

    更新日期：1998-03-31 00:00:00

  • Oligomeric state and mode of self-association of Thermotoga maritima ribosomal stalk protein L12 in solution.

    abstract：:The "stalk" of the prokaryotic 50S ribosomal subunit is comprised of four copies of the protein L7/L12. In Escherichia coli, L7/L12 is a dimeric protein at micromolar concentrations, which is able to undergo rapid subunit exchange. A recent structural study indicated a tetrameric arrangement of the L12 proteins isolat...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi048015n

    authors： Moens PD,Wahl MC,Jameson DM

    更新日期：2005-03-08 00:00:00

  • Identification of active site residues essential to 4-chlorobenzoyl-coenzyme A dehalogenase catalysis by chemical modification and site directed mutagenesis.

    abstract：:4-Chlorobenzoyl-coenzyme A (4-CBA-CoA) dehalogenase catalyzes the hydrolysis of 4-CBA-CoA to 4-hydroxybenzoyl-coenzyme A (4-HBA-CoA) via a nucleophilic aromatic substitution pathway involving the participation of an active site carboxylate side chain in covalent catalysis. In this paper we report on the identification...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9609533

    authors： Yang G,Liu RQ,Taylor KL,Xiang H,Price J,Dunaway-Mariano D

    更新日期：1996-08-20 00:00:00

  • Deletion of exon 21 of the insulin receptor eliminates tyrosine kinase activity but preserves mitogenic signaling.

    abstract：:To study the function of exon 21 of the insulin receptor, a mutant human insulin receptor lacking this domain was constructed. The mutant HIR delta E21 cDNA was transfected into Rat-1 fibroblasts and stable cell lines were selected. The HIR delta E21 receptors were expressed on the cell surface, and they bound insulin...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00212a021

    authors： Rolband GC,Williams JF,Webster NJ,Hsu D,Olefsky JM

    更新日期：1993-12-14 00:00:00