Abstract:
:The 18 kDa translocator protein (TSPO), formerly known as the peripheral-type benzodiazepine receptor, has been reported to be closely associated with the mitochondrial permeability transition pore (MPTP). TSPO is believed to exert pro-apoptotic functions via modulation of MPTP opening. Cobalt chloride (CoCl(2)), which is sometimes used as a hypoxia mimicking agent, is also known to be able to induce apoptosis. One of our questions was whether CoCl(2) may induce apoptosis via the TSPO. To address this question, we used the U118MG human glioblastoma cell line. We applied the specific TSPO ligand, PK 11195, as well as TSPO knockdown with siRNA and studied their influence on the effects of CoCl(2) on cell death, including activation of the mitochondrial apoptosis pathway. To assay TSPO expression, we applied binding assays and Western blotting to whole cell homogenates and mitochondrial fractions. To assay activation of the mitochondrial apoptosis pathway, including some of the cellular mechanisms involved, we determined the incidence of collapse of the mitochondrial membrane potential (Deltapsi(m)) and cardiolipin oxidation and measured the level of DNA fragmentation to assay apoptotic rates. We found that the TSPO ligand, PK 11195, significantly counteracted induction of cell death by 0.4 mM CoCl(2), including apoptosis, collapse of the Deltapsi(m), and cardiolipin oxidation. Moreover, we found that TSPO knockdown with siRNA fully protected against mentioned cell death mechanisms. Thus, we found that the TSPO is required for cell death induction by CoCl(2), including apoptosis. In conclusion, our studies show that activation of TSPO by CoCl(2) application is required for ROS generation, leading to cardiolipin oxidation, and collapse of the Deltapsi(m), as induced by CoCl(2).
journal_name
Biochemistryjournal_title
Biochemistryauthors
Zeno S,Zaaroor M,Leschiner S,Veenman L,Gavish Mdoi
10.1021/bi900064tsubject
Has Abstractpub_date
2009-06-02 00:00:00pages
4652-61issue
21eissn
0006-2960issn
1520-4995journal_volume
48pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::A newly reported strain of Vibrio cholerae, known as strain O139 Bengal, is the first instance of an encapsulated strain that has caused epidemic cholera. The O-antigenic capsule is the critical antigen for protective immunity. Since mapping of the antigenic epitopes will assist in the development of a protein conjuga...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9910272
更新日期:1999-09-14 00:00:00
abstract::Several common links between the structural chemistry of the chitolipooligosaccharides of Rhizobium and the general rhizobial membrane lipid and lipopolysaccharide chemistry of these bacteria have been uncovered. Aspects of common chemistry include sulfation, methylation, and the position and extent of fatty acyl chai...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00013a040
更新日期:1995-04-04 00:00:00
abstract::Pig kidney general acyl-CoA dehydrogenases forms the blue neutral radical on dithionite or photochemical reduction (Thorpe, C., Matthews, R. G., & Williams, C. H. (1979) Biochemistry 18, 331-337] in accord with its classification as a flavoprotein dehydrogenase. However, dithionite reduction of the enzyme in the prese...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00520a024
更新日期:1981-08-18 00:00:00
abstract::Oligodeoxynucleotides modified site-specifically with dG-(+)-trans- and dG-(+)-cis-anti-BPDE (7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene) or dG-(-)-trans- and dG-(-)-cis-anti-BPDE were used as templates in primer extension reactions catalyzed by the Klenow fragment of Escherichia coli DNA polymerase I....
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00080a027
更新日期:1993-07-27 00:00:00
abstract::B₁₂-dependent enzymes employ radical species with exceptional prowess to catalyze some of the most chemically challenging, thermodynamically unfavorable reactions. However, dealing with highly reactive intermediates is an extremely demanding task, requiring sophisticated control strategies to prevent unwanted side rea...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500050k
更新日期:2014-06-17 00:00:00
abstract::Locusta migratoria apolipophorin III (apoLp-III) is a helix bundle exchangeable apolipoprotein that reversibly binds to lipoprotein surfaces. Structural reorganization of its five amphipathic alpha-helices enables the transition from the lipid-free to lipid-bound state. ApoLp-III-induced transformation of dimyristoylp...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi010410f
更新日期:2001-06-26 00:00:00
abstract::Two extremely potent inhibitors of citrate synthase, carboxyl and primary amide analogues of acetyl coenzyme A, have been synthesized. The ternary complexes of these inhibitors with oxaloacetate and citrate synthase have been crystallized and their structures analyzed at 1.70- and 1.65-A resolution, respectively. The ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00191a002
更新日期:1994-06-28 00:00:00
abstract::The doublet at 850 and 830 cm-1 in the Raman spectra of proteins containing tyrosyl residues has been examined as to its origin and the relation of its components to the environment of the phenyl ring, the state of the phenolic hydroxyl group, and the conformation of the amino acid backbone. Raman spectral studies on ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00693a014
更新日期:1975-11-04 00:00:00
abstract::The crystal structures of the guanosine 5'-monophosphate (GMP) and inosine 5'-monophosphate (IMP) complexes of Toxoplasma gondii hypoxanthine-guanine phosphoribosyltransferase (HGPRT) have been determined at 1.65 and 1.90 A resolution. These complexes, which crystallize in space groups P2(1) (a = 65.45 A, b = 90.84 A,...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990507q
更新日期:1999-11-02 00:00:00
abstract::While the insertion of a hydrophobic peptide or membrane protein segment into the bilayer can be spontaneous and driven mainly by the hydrophobic effect, anionic lipids, which comprise ca. 20% of biological membranes, provide a source of electrostatic attractions for binding of proteins/peptides into membranes. To unr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi970030n
更新日期:1997-05-06 00:00:00
abstract::The mechanism by which some hydrophobic molecules such as steroids and free fatty acids (FFA) act as noncompetitive inhibitors of the nicotinic acetylcholine receptor (AChR) is still not known. In the present work, we employ Förster resonance energy transfer (FRET) between the intrinsic fluorescence of membrane-bound ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061388z
更新日期:2007-03-20 00:00:00
abstract::The Cyt toxins produced by the bacteria Bacillus thuringiensis show insecticidal activity against some insects, mainly dipteran larvae, being able to kill mosquitoes and black flies. However, they also possess a general cytolytic activity in vitro, showing hemolytic activity in red blood cells. These proteins are comp...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi101239r
更新日期:2011-01-25 00:00:00
abstract::The complete amino acid sequence of the 4Fe-4S ferredoxin from the thermophilic bacterium Clostridium thermoaceticum has been determined. The protein is extremely thermostable and is the only known clostridial ferredoxin to contain a single [4Fe-4S] cluster. The sequence totals 63 residues and includes the first trypt...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00257a007
更新日期:1982-07-06 00:00:00
abstract::Although the synthesis of RNA from a DNA template is (and must be) a generally very stable process to enable transcription of kilobase transcripts, it has long been known that during initial transcription of the first 8-10 bases of RNA complexes are relatively unstable, leading to the release of short abortive RNA tra...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200620q
更新日期:2011-08-16 00:00:00
abstract::Carbonic anhydrase V (CA V) is a mitochondrial enzyme that catalyzes the hydration of CO2 to produce bicarbonate and a proton. The catalytic properties of wild-type murine CA V suggest the presence of a proton shuttle residue having pKa = 9.2, the role of which is to transfer a proton from zinc-bound water to solution...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9608018
更新日期:1996-09-10 00:00:00
abstract::Myeloperoxidase plays a fundamental role in oxidant production by neutrophils. The enzyme uses hydrogen peroxide to oxidize chloride (Cl-), bromide (Br-), iodide (I-), and the pseudohalide thiocyanate (SCN-) to their respective hypohalous acids. This study for the first time presents transient kinetic measurements of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9818772
更新日期:1998-12-22 00:00:00
abstract::The transmission of regulatory signals between the alpha- and beta-subunits of the tryptophan synthase alpha 2 beta 2 complex from Salmonella typhimurium has been investigated by monitoring the luminescence properties of the enzyme in the presence and in the absence of the alpha-subunit ligand DL-alpha-glycerol 3-phos...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00148a014
更新日期:1992-08-25 00:00:00
abstract::Hydrogen bonds which form between a hydrogen bond donor and an aromatic ring as acceptor are thought to contribute to the stability and function of proteins. We have tested the function of such an interaction in a highly homologous pair of proteins, cellular retinol-binding protein (CRBP) and cellular retinol-binding ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00035a018
更新日期:1995-09-05 00:00:00
abstract::Studies of intragenomic homologues in bacterial genomes can provide valuable insights into functional divergence. Three GTP cyclohydrolase II homologues in the Streptomyces coelicolor genome have been shown to catalyze two related but distinct reactions [Spoonamore, J. E., Dahlgran, A. L., Jacobsen, N. E., and Bandari...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi702263z
更新日期:2008-02-26 00:00:00
abstract::Protein disulfide-isomerase, a protein localized to the lumen of the endoplasmic reticulum of eukaryotic cells, catalyzes the posttranslational formation and rearrangement of protein disulfide bonds. As isolated from bovine liver, the enzyme contains 0.8 free sulfhydryl group per mole of protein monomer and 3.1 disulf...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00444a023
更新日期:1989-09-05 00:00:00
abstract::Flash-induced Fourier transform infrared (FTIR) difference spectra for the four-step S-state cycle and the effects of global (15)N- and (13)C-isotope labeling on the difference spectra were examined for the first time in the mid- to low-frequency (1200-800 cm(-1)) as well as the mid-frequency (1700-1200 cm(-1)) region...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0362323
更新日期:2004-06-15 00:00:00
abstract::The quantitative tissue specificity of the high mobility group (HMG) chromosomal proteins was investigated. Perchloric acid (PCA) extracts of four different chicken tissues and erythrocytes contained three proteins which comigrated on NaDodSO4-polyacrylamide gels with the HMG's 1,2, and E from erythrocyte nuclei. Thes...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00560a003
更新日期:1980-09-16 00:00:00
abstract::Messenger RNA degradation is an important component of overall gene expression. During the final step of eukaryotic mRNA degradation, exoribonuclease 1 (Xrn1) carries out 5' → 3' processive, hydrolytic degradation of RNA molecules using divalent metal ion catalysis. To initiate studies of the 5' → 3' RNA decay machine...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b01035
更新日期:2020-04-21 00:00:00
abstract::Collagen is a multifunctional protein, serving as a structural scaffold and a modulator of cellular responses. Prior work has identified distinct regions from several collagen types that promote cell adhesion, spreading, migration, and signal transduction. One of these regions, alpha1(IV)1263-1277 from type IV collage...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi012071w
更新日期:2002-05-14 00:00:00
abstract::Nanosecond to millisecond conformational fluctuations of Zn-substituted cytochrome c (ZnCytc) have been studied by the time-resolved transient hole-burning method. The investigation of low-temperature dynamics has been made on the ZnCytc solution sample in a water-glycerol mixture. The conformational fluctuations in t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981568b
更新日期:1999-02-09 00:00:00
abstract::X-ray studies of the proofreading 3',5'-exonuclease site of the large (Klenow) fragment of DNA polymerase I have detected a binuclear metal complex consisting of a pentacoordinate metal (site A) which shares a ligand, Asp-355, with an octahedral metal (site B) [Freemont, P. S., Friedman, J. M., Beese, L. S., Sanderson...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00110a012
更新日期:1991-11-19 00:00:00
abstract::Two thiol-activated endopeptidases with pH optima near pH 7.5 were isolated from the supernatant fraction of rabbit brain homogenates by DEAE-cellulose chromatography, gel filtration and isoelectrofocusing. Peptide bond hydrolysis was measured quantitatively by ion-exchange chromatography with an amino acid analyzer. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00654a026
更新日期:1976-05-04 00:00:00
abstract::The intense intrinsic fluorescence emissions from several clinically relevant camptothecin drugs have been exploited in order to determine (1) the structural basis of drug binding to lipid bilayers, (2) the lipid bilayer stability of each drug's alpha-hydroxylactone moiety, a pharmacophore which is essential for antit...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00071a010
更新日期:1993-05-25 00:00:00
abstract::The phosphocarrier protein HPr of the phosphoenolpyruvate-dependent sugar transport system of Escherichia coli can exist in a phosphorylated and a nonphosphorylated form. During phosphorylation, the phosphoryl group is carried on a histidine residue, His15. The hydrogen-bonding state of this histidine was examined wit...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00487a026
更新日期:1990-09-04 00:00:00
abstract::High-resolution 2D NMR spectra of the duplex CGCGAATTCGCG with deoxyribose sugars but with the normal phosphodiester linker replaced by an N3' --> P5' phosphoramidate (NP) group have been used to establish a solution structure for the duplex. Distance, angle, and base pair hydrogen-bonding constraints were used to ref...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi980711y
更新日期:1998-09-01 00:00:00