A second photochromic bacteriophytochrome from Synechocystis sp. PCC 6803: spectral analysis and down-regulation by light.


:It now appears that photosynthetic prokaryotes and lower eukaryotes possess higher plant phytochrome-like proteins. In this work, a second phytochrome-like gene was isolated, in addition to the recently identified Cph1 phytochrome, from the Synechocystis sp. PCC 6803, and its gene product was characterized photochemically. The open reading frame sll0821 (designated cph2 in this work) has structural characteristics similar to those of the plant phytochromes and the Synechocystis Cph1 with high amino acid sequence homology in the N-terminal chromophore binding domain. The predicted Cph2 protein consists of 1276 amino acids with a calculated molecular mass of 145 kDa. Interestingly, the Cph2 protein has two putative chromophore binding domains, one around Cys-129 and the other around Cys-1022. The Cph2 was overexpressed in E. coli as an Intein/CBD (chitin binding domain) fusion and in vitro reconstituted with phycocyanobilin (PCB) or phytochromobilin (PPhiB). Both the Cph2-PCB and Cph2-PPhiB adducts showed the typical photochromic reversibility with the difference spectral maxima at 643/690 and 655/701 nm, respectively. The Cys-129 was confirmed to be the chromophore binding residue by in vitro mutagenesis and Zn(2+) fluorescence. The microenvironment of the chromophore in Cph2 seems to be similar to that in plant phytochromes. The cph2 gene expression was dark-induced and down-regulated to a basal level by light, like the cph1 gene. These observations suggest that Synechocystis species have multiple photosensory proteins, probably with distinct roles, as in higher plants.






Park CM,Kim JI,Yang SS,Kang JG,Kang JH,Shim JY,Chung YH,Park YM,Song PS




Has Abstract


2000-09-05 00:00:00














  • Thermodynamic characterization of nucleoplasmin unfolding: interplay between function and stability.

    abstract::The unfolding equilibrium of recombinant (rNP) and natural variants of nucleoplasmin (NP) from Xenopus laevis has been analyzed using biochemical and spectroscopic techniques. In the presence of denaturing concentrations of guanidinium salts (GuHCl and GuSCN), both domains, core and tail, of the rNP pentamer unfold as...


    pub_type: 杂志文章


    authors: Franco G,Bañuelos S,Falces J,Muga A,Urbaneja MA

    更新日期:2008-07-29 00:00:00

  • Characterization of multiple forms of the Ah receptor: recognition of a dioxin-responsive enhancer involves heteromer formation.

    abstract::We have employed a combination of gel retardation, protein-DNA cross-linking, and protein-protein cross-linking techniques to further examine the 2,3,7,8-tetrachlorodibenzo-p- dioxin-(TCDD-) dependent changes in the Ah receptor that result in a DNA-binding conformation. Gel retardation analysis of DNA-Sepharose chroma...


    pub_type: 杂志文章


    authors: Gasiewicz TA,Elferink CJ,Henry EC

    更新日期:1991-03-19 00:00:00

  • Denaturation of cytochrome P450 2B1 by guanidine hydrochloride and urea: evidence for a metastable intermediate state of the active site.

    abstract::A metastable intermediate was found in the course of the denaturation of purified cytochrome P450 2B1 by increasing concentrations of guanidine hydrochloride (GuHCl). The metastable intermediate has no or low absorbance at 450 nm in the reduced carbon monoxide difference spectrum and has no absorbance at 420 nm. The i...


    pub_type: 杂志文章


    authors: Yu XC,Shen S,Strobel HW

    更新日期:1995-04-25 00:00:00

  • Water structural changes in lumirhodopsin, metarhodopsin I, and metarhodopsin II upon photolysis of bovine rhodopsin: analysis by Fourier transform infrared spectroscopy.

    abstract::Difference Fourier transform infrared spectra of lumirhodopsin, metarhodopsin I, and metarhodopsin II versus rhodopsin were recorded with hydrated films of bovine rod outer segments at 200, 240, and 270 K. In the region between 3700 and 3450 cm-1, the O-H stretching vibrational bands of water were identified by H(2)18...


    pub_type: 杂志文章


    authors: Maeda A,Ohkita YJ,Sasaki J,Shichida Y,Yoshizawa T

    更新日期:1993-11-16 00:00:00

  • Three-dimensional model for the membrane domain of Escherichia coli leader peptidase based on disulfide mapping.

    abstract::We have mapped the interface between the two transmembrane alpha-helices in the membrane domain of the Escherichia coli enzyme leader peptidase by analyzing disulfides formed between pairs of cysteine residues introduced near their respective periplasmic ends. The interface is formed primarily from aliphatic amino aci...


    pub_type: 杂志文章


    authors: Whitley P,Nilsson L,von Heijne G

    更新日期:1993-08-24 00:00:00

  • Serine92 (F7) contributes to the control of heme reactivity and stability in myoglobin.

    abstract::The effects of mutation of the conserved serine92 residue to alanine, valine, and leucine in pig myoglobin have been determined. In myoglobin crystal structures, the hydroxyl group of serine92 is within hydrogen-bonding distance of the N delta-H of histidine93, whose N epsilon coordinates the iron atom of the heme pro...


    pub_type: 杂志文章


    authors: Smerdon SJ,Krzywda S,Wilkinson AJ,Brantley RE Jr,Carver TE,Hargrove MS,Olson JS

    更新日期:1993-05-18 00:00:00

  • Physical properties of defined lipopolysaccharide salts.

    abstract::The electron spin resonance probes 5-doxylstearate and 4-(dodecyldimethylammonio)-1-oxy-2,2,6,6-tetramethylpiperidine bromide were used to characterize the fluidity of the acyl chain and head-group regions, respectively, of defined salts of lipopolysaccharide (LPS) from Escherichia coli K12. The removal of the weakly ...


    pub_type: 杂志文章


    authors: Coughlin RT,Haug A,McGroarty EJ

    更新日期:1983-04-12 00:00:00

  • A heuristic approach to the analysis of enzymic catalysis: reaction of delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-alpha-aminobutyrate and delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-allylglycine catalyzed by isopenicillin N synthase isozymes.

    abstract::Isopenicillin N synthase (IPNS) catalyzes the oxidative cyclization of delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valine to isopenicillin N. It is proposed that the multiple products produced from certain substrate analogues result from pathway branching after formation of a ferryl oxene intermediate. We have been int...


    pub_type: 杂志文章


    authors: Blackburn JM,Sutherland JD,Baldwin JE

    更新日期:1995-06-06 00:00:00

  • Conformational preferences of synthetic peptides derived from the immunodominant site of the circumsporozoite protein of Plasmodium falciparum by 1H NMR.

    abstract::Proton nuclear magnetic resonance and ultraviolet circular dichroism spectroscopy have been used to probe the conformational ensemble of the tandemly repeating tetrapeptide unit of the circumsporozoite coat protein of the malaria parasite Plasmodium falciparum. Peptides based on the Asn-Ala-Asn-Pro and Asn-Pro-Asn-Ala...


    pub_type: 杂志文章


    authors: Dyson HJ,Satterthwait AC,Lerner RA,Wright PE

    更新日期:1990-08-28 00:00:00

  • Characterization and topology of the membrane domain Nqo10 subunit of the proton-translocating NADH-quinone oxidoreductase of Paracoccus denitrificans.

    abstract::The proton-translocating NADH-quinone oxidoreductase (NDH-1) of Paracoccus denitrificans is composed of 14 different subunits (Nqo1-Nqo14). Of these, seven subunits (Nqo7, Nqo8, and Nqo10-14) which are equivalent to the mitochondrial DNA-encoded subunits of complex I constitute the membrane segment of the enzyme compl...


    pub_type: 杂志文章


    authors: Kao MC,Di Bernardo S,Matsuno-Yagi A,Yagi T

    更新日期:2003-04-22 00:00:00

  • Electrostatic optimization of the conformational energy landscape in a metamorphic protein.

    abstract::The equilibrium unfolding reaction of Ltn, a metamorphic C-class chemokine, was monitored by tryptophan fluorescence to determine unfolding free energies. Measurements revealed that addition of 150 mM NaCl stabilized the Ltn chemokine fold by approximately 1 kcal/mol. Specific mutations involving Arg23 and Arg43 also ...


    pub_type: 杂志文章


    authors: Tyler RC,Wieting JC,Peterson FC,Volkman BF

    更新日期:2012-11-13 00:00:00

  • Inactivation of soybean lipoxygenase 1 by 12-iodo-cis-9-octadecenoic acid.

    abstract::12-Iodo-cis-9-octadecenoic acid (12-IODE) is a time-dependent, irreversible inactivator of soybean lipoxygenase 1. The rate of inactivation is independent of 12-IODE concentration above 20 microM and is half-maximal at about 4 microM. Inactivation by 12-IODE requires lipid hydroperoxide, which must be present even aft...


    pub_type: 杂志文章


    authors: Rotenberg SA,Grandizio AM,Selzer AT,Clapp CH

    更新日期:1988-11-29 00:00:00

  • An N-terminal deletion mutant of estrogen receptor exhibits increased synergism with upstream activators and enhanced binding to the estrogen response element.

    abstract::To study the role of the N-terminal region of the estrogen receptor (ER) in transcription activation and in DNA binding, we constructed a mutant of the Xenopus laevis ER which lacks amino acids 1-159 (XER160/586). In transient transfections, XER160/586 exhibited < 10% of the activity of wild-type XER on a synthetic pr...


    pub_type: 杂志文章


    authors: Xing H,Mattick S,Lew D,Shapiro DJ

    更新日期:1995-03-28 00:00:00

  • Thermodynamics of nucleotide and inhibitor binding to wild-type and ispinesib-resistant forms of human kinesin spindle protein.

    abstract::Current antimitotic cancer chemotherapy based on vinca alkaloids and taxanes target tubulin, a protein required not only for mitotic spindle formation but also for the overall structural integrity of terminally differentiated cells. Among many innovations targeting specific mitotic events, inhibition of motor enzymes ...


    pub_type: 杂志文章


    authors: Sheth PR,Basso A,Duca JS,Lesburg CA,Ogas P,Gray K,Nale L,Mannarino AF,Prongay AJ,Le HV

    更新日期:2009-11-24 00:00:00

  • Chemical synthesis of LNA-2-thiouridine and its influence on stability and selectivity of oligonucleotide binding to RNA.

    abstract::Hybridization to RNA is important for many applications, including antisense therapeutics, RNA interference, and microarray screening. Similar thermodynamic stabilities of A-U and G-U base pairs result in difficulties in selective binding to RNA. Moreover, A-U pairs are weaker than G-C pairs so that binding is sometim...


    pub_type: 杂志文章


    authors: Carlucci M,Kierzek E,Olejnik A,Turner DH,Kierzek R

    更新日期:2009-11-24 00:00:00

  • RNA phosphorylation: a polynucleotide kinase function in mouse L cell nuclei.

    abstract::Analysis of [gamma-32P]ATP in vitro labeled nuclear RNA shows transfer of gamma-32P from ATP to form the 5'-terminal monophosphate for large RNA molecules. This finding is an actively transcribing nuclear system capable of guanylation and methylation reactions indicates that polynucleotide kinase activity in the eukar...


    pub_type: 杂志文章


    authors: Winicov I

    更新日期:1977-09-20 00:00:00

  • Location and interactions of phospholipid and cholesterol in human low density lipoprotein from 31P nuclear magnetic resonance.

    abstract::The major phospholipids, phosphatidylcholine and spingomyelin, of low density lipoprotein (LDL) are accessible to small amounts of Pr3+, suggesting that the head groups of all mobile phospholipids are on the surface of the particle in contact with the aqueous medium. The major source of the nuclear Overhauser effect e...


    pub_type: 杂志文章


    authors: Yeagle PL,Martin RB,Pottenger L,Langdon RG

    更新日期:1978-07-11 00:00:00

  • Picosecond dynamics of tyrosine side chains in proteins.

    abstract::To probe the details of small amplitude motions in proteins, a dynamical analysis of the orientation fluctuations of two tyrosine side chains in the bovine pancreatic trypsin inhibitor is presented. Detailed results are given for the time history and correlation functions obtained for the ring motion from a molecular ...


    pub_type: 杂志文章


    authors: McCammon JA,Wolynes PG,Karplus M

    更新日期:1979-03-20 00:00:00

  • Ligand migration and binding in nonsymbiotic hemoglobins of Arabidopsis thaliana.

    abstract::We have studied carbon monoxide (CO) migration and binding in the nonsymbiotic hemoglobins AHb1 and AHb2 of Arabidopsis thaliana using Fourier transform infrared (FTIR) spectroscopy combined with temperature derivative spectroscopy (TDS) at cryogenic temperatures. Both proteins have similar amino acid sequences but di...


    pub_type: 杂志文章


    authors: Nienhaus K,Dominici P,Astegno A,Abbruzzetti S,Viappiani C,Nienhaus GU

    更新日期:2010-09-07 00:00:00

  • Aluminum exchange between citrate and human serum transferrin and interaction with transferrin receptor 1.

    abstract::The kinetics and thermodynamics of Al(III) exchange between aluminum citrate (AlL) and human serum transferrin were investigated in the 7.2-8.9 pH range. The C-site of human serum apotransferrin in interaction with bicarbonate removes Al(III) from Al citrate with an exchange equilibrium constant K1 = (2.0 +/- 0.6) x 1...


    pub_type: 杂志文章


    authors: Hémadi M,Miquel G,Kahn PH,El Hage Chahine JM

    更新日期:2003-03-18 00:00:00

  • Novel allosteric conformation of human HB revealed by the hydration and anion effects on O(2) binding.

    abstract::The effect of anions on the stability of different functional conformations of Hb is examined through the determination of the dependence of O(2) affinity on water activity (a(w)). The control of a(w) is effected by varying the sucrose osmolal concentration in the bathing solution according to the "osmotic stress" met...


    pub_type: 杂志文章


    authors: Colombo MF,Seixas FA

    更新日期:1999-09-07 00:00:00

  • FTIR study of a nonclassical dT10*dA10-dT10 intramolecular triple helix.

    abstract::Many early investigations on triple helices have been devoted to the study of the triplex formed by dT*dA-dT base triplets in which the third strand is oriented parallel to the dA strand. We now describe an intramolecular triple helix with dT*dA-dT base triplets in which the pyrimidine third strand is oriented antipar...


    pub_type: 杂志文章


    authors: Dagneaux C,Liquier J,Taillandier E

    更新日期:1995-11-14 00:00:00

  • The solution structure of Bacillus anthracis dihydrofolate reductase yields insight into the analysis of structure-activity relationships for novel inhibitors.

    abstract::There is a significant need for new therapeutics to treat infections caused by the biodefense agent Bacillus anthracis. In pursuit of drug discovery against this organism, we have developed novel propargyl-linked inhibitors that target the essential enzyme dihydrofolate reductase (DHFR) from B. anthracis. Previously, ...


    pub_type: 杂志文章


    authors: Beierlein JM,Deshmukh L,Frey KM,Vinogradova O,Anderson AC

    更新日期:2009-05-19 00:00:00

  • Growth factor receptor-bound protein 14 undergoes light-dependent intracellular translocation in rod photoreceptors: functional role in retinal insulin receptor activation.

    abstract::Growth factor receptor-bound protein 14 (Grb14) is involved in growth factor receptor tyrosine kinase signaling. Here we report that light causes a major redistribution of Grb14 among the individual subcellular compartments of the retinal rod photoreceptor. Grb14 is localized predominantly to the inner segment, nuclea...


    pub_type: 杂志文章


    authors: Rajala A,Daly RJ,Tanito M,Allen DT,Holt LJ,Lobanova ES,Arshavsky VY,Rajala RV

    更新日期:2009-06-23 00:00:00

  • Effects of metal ligation and oxygen on the reversibility of the thermal denaturation of Pseudomonas aeruginosa azurin.

    abstract::Thermodynamic equilibrium transition models in DSC are only applicable to reversible processes, but reversibility of the thermal transitions of proteins is comparatively rare because of intermolecular aggregation of denatured proteins and the degradation that occurs at high temperatures. The cupredoxin azurin from Pse...


    pub_type: 杂志文章


    authors: Sandberg A,Leckner J,Shi Y,Schwarz FP,Karlsson BG

    更新日期:2002-01-22 00:00:00

  • Investigating Pharmacological Targeting of G-Quadruplexes in the Human Malaria Parasite.

    abstract::The unique occurrence of G-quadruplexes in the AT-rich genome of human malaria parasite Plasmodium falciparum provides hints about their critical roles in parasite survival, pathogenesis, and host immune evasion. An intriguing question is whether these noncanonical structures can serve as molecular targets for small m...


    pub_type: 杂志文章


    authors: Anas M,Sharma R,Dhamodharan V,Pradeepkumar PI,Manhas A,Srivastava K,Ahmed S,Kumar N

    更新日期:2017-12-26 00:00:00

  • Structural basis of DNA recognition and mechanism of quadruplex formation by the beta subunit of the Oxytricha telomere binding protein.

    abstract::Interactions of the beta subunit of the Oxytricha nova telomere binding protein with the telomeric DNA sequences, d(T4G4)2 and dT6(T4G4)2, have been investigated in vitro using Raman and fluorescence spectroscopies. Raman difference spectra show that the beta subunit binds to both d(T4G4)2 and dT6(T4G4)2 but promotes ...


    pub_type: 杂志文章


    authors: Laporte L,Thomas GJ Jr

    更新日期:1998-02-03 00:00:00

  • Synthesis of dihydrothymidine and thymidine glycol 5'-triphosphates and their ability to serve as substrates for Escherichia coli DNA polymerase I.

    abstract::5,6-Dihydrothymidine 5'-triphosphate (DHdTTP) was synthesized by catalytic hydrogenation of thymidine 5'-triphosphate (dTTP). Thymidine glycol 5'-triphosphate (dTTP-GLY) was prepared by bromination of dTTP followed by treatment with Ag2O. The modified nucleotides were extensively purified by anion-exchange high-perfor...


    pub_type: 杂志文章


    authors: Ide H,Melamede RJ,Wallace SS

    更新日期:1987-02-10 00:00:00

  • Hypochlorous acid reacts with the N-terminal methionines of proteins to give dehydromethionine, a potential biomarker for neutrophil-induced oxidative stress.

    abstract::Electrophilic halogenating agents, including hypohalous acids and haloamines, oxidize free methionine and the N-terminal methionines of peptides and proteins (e.g., Met-1 of anti-inflammatory peptide 1 and ubiquitin) to produce dehydromethionine (a five-membered isothiazolidinium heterocycle). Amide derivatives of met...


    pub_type: 杂志文章


    authors: Beal JL,Foster SB,Ashby MT

    更新日期:2009-11-24 00:00:00

  • Conformations of primary amphipathic carrier peptides in membrane mimicking environments.

    abstract::Two peptides designed for drug delivery were generated by the combination of a signal peptide with a nuclear localization sequence and are shown to facilitate the cellular internalization of small molecules which are covalently linked to these peptides. In order to understand the mechanism of internalization, the conf...


    pub_type: 杂志文章


    authors: Chaloin L,Vidal P,Heitz A,Van Mau N,Méry J,Divita G,Heitz F

    更新日期:1997-09-16 00:00:00