当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > A second photochromic bacteriophytochrome from Synechocystis sp. PCC 6803: spectral analysis and down-regulation by light.

A second photochromic bacteriophytochrome from Synechocystis sp. PCC 6803: spectral analysis and down-regulation by light.

Abstract：

:It now appears that photosynthetic prokaryotes and lower eukaryotes possess higher plant phytochrome-like proteins. In this work, a second phytochrome-like gene was isolated, in addition to the recently identified Cph1 phytochrome, from the Synechocystis sp. PCC 6803, and its gene product was characterized photochemically. The open reading frame sll0821 (designated cph2 in this work) has structural characteristics similar to those of the plant phytochromes and the Synechocystis Cph1 with high amino acid sequence homology in the N-terminal chromophore binding domain. The predicted Cph2 protein consists of 1276 amino acids with a calculated molecular mass of 145 kDa. Interestingly, the Cph2 protein has two putative chromophore binding domains, one around Cys-129 and the other around Cys-1022. The Cph2 was overexpressed in E. coli as an Intein/CBD (chitin binding domain) fusion and in vitro reconstituted with phycocyanobilin (PCB) or phytochromobilin (PPhiB). Both the Cph2-PCB and Cph2-PPhiB adducts showed the typical photochromic reversibility with the difference spectral maxima at 643/690 and 655/701 nm, respectively. The Cys-129 was confirmed to be the chromophore binding residue by in vitro mutagenesis and Zn(2+) fluorescence. The microenvironment of the chromophore in Cph2 seems to be similar to that in plant phytochromes. The cph2 gene expression was dark-induced and down-regulated to a basal level by light, like the cph1 gene. These observations suggest that Synechocystis species have multiple photosensory proteins, probably with distinct roles, as in higher plants.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Park CM,Kim JI,Yang SS,Kang JG,Kang JH,Shim JY,Chung YH,Park YM,Song PS

doi

10.1021/bi992831r

subject

Has Abstract

pub_date

2000-09-05 00:00:00

pages

10840-7

issue

35

eissn

0006-2960

issn

1520-4995

pii

bi992831r

journal_volume

39

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Solution structure of the E-domain of staphylococcal protein A.

    abstract：:The E-domain of staphylococcal protein A is one of five homologous IgG-binding domains designated E, D, A, B, and C that comprise the extracellular portion of protein A. The E-domain binds tightly to Fc fragments of IgG and binds certain Fv fragments with micromolar affinity. To explore further the structural features...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi961409x

    authors： Starovasnik MA,Skelton NJ,O'Connell MP,Kelley RF,Reilly D,Fairbrother WJ

    更新日期：1996-12-03 00:00:00

  • Glutamate 189 of the D1 polypeptide modulates the magnetic and redox properties of the manganese cluster and tyrosine Y(Z) in photosystem II.

    abstract：:Recent models for water oxidation in photosystem II postulate that the tyrosine Y(Z) radical, Y(Z)(*), abstracts both an electron and a proton from the Mn cluster during one or more steps in the catalytic cycle. This coupling of proton- and electron-transfer events is postulated to provide the necessary driving force ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi992749w

    authors： Debus RJ,Campbell KA,Pham DP,Hays AM,Britt RD

    更新日期：2000-05-30 00:00:00

  • Molecular Basis for Allosteric Inhibition of GTP-Bound H-Ras Protein by a Small-Molecule Compound Carrying a Naphthalene Ring.

    abstract：:The ras oncogene products (H-Ras, K-Ras, and N-Ras) have been regarded as some of the most promising targets for anticancer drug discovery because their activating mutations are frequently found in human cancers. Nonetheless, molecular targeted therapy for them is currently unavailable. Here, we report the discovery o...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b00680

    authors： Matsumoto S,Hiraga T,Hayashi Y,Yoshikawa Y,Tsuda C,Araki M,Neya M,Shima F,Kataoka T

    更新日期：2018-09-11 00:00:00

  • Fo membrane domain of ATP synthase from bovine heart mitochondria: purification, subunit composition, and reconstitution with F1-ATPase.

    abstract：:The Fo membrane domain of the F1Fo-ATP synthase complex has been purified from bovine heart mitochondria. The purification procedure involves the removal of peripheral membrane proteins, including F1-ATPase, from submitochondrial particles with guanidine hydrochloride, followed by extraction of Fo and other membrane p...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00191a026

    authors： Collinson IR,Runswick MJ,Buchanan SK,Fearnley IM,Skehel JM,van Raaij MJ,Griffiths DE,Walker JE

    更新日期：1994-06-28 00:00:00

  • beta-Lactamase proceeds via an acyl-enzyme intermediate. Interaction of the Escherichia coli RTEM enzyme with cefoxitin.

    abstract：:The use of cefoxitin, a poor substrate of the RTEM beta-lactamase, has allowed the kinetic and spectroscopic characterization of a covalent acyl-enzyme intermediate in the enzyme-catalyzed reaction. The rate of reappearance of catalytic activity in an enzyme sample diluted from an incubation with cefoxitin is nearly i...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00554a012

    authors： Fisher J,Belasco JG,Khosla S,Knowles JR

    更新日期：1980-06-24 00:00:00

  • Structural Studies of AAV2 Rep68 Reveal a Partially Structured Linker and Compact Domain Conformation.

    abstract：:Adeno-associated virus (AAV) nonstructural proteins Rep78 and Rep68 carry out all DNA transactions that regulate the AAV life cycle. They share two multifunctional domains: an N-terminal origin binding/nicking domain (OBD) from the HUH superfamily and a SF3 helicase domain. A short linker of ∼20 amino acids that is cr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00610

    authors： Musayev FN,Zarate-Perez F,Bardelli M,Bishop C,Saniev EF,Linden RM,Henckaerts E,Escalante CR

    更新日期：2015-09-29 00:00:00

  • Self-assembly of aqueous bilirubin ditaurate, a natural conjugated bile pigment, to contraposing enantiomeric dimers and M(-) and P(+) tetramers and their selective hydrophilic disaggregation by monomers and micelles of bile salts.

    abstract：:The solution behavior of bilirubin ditaurate (BDT), the first naturally occurring conjugated bile pigment to be physically and chemically characterized, was assessed in aqueous solution and in monomeric and micellar solutions of common taurine-conjugated bile salts (BS). Analytical ultracentrifugation revealed that BD...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi501251v

    authors： Neubrand MW,Carey MC,Laue TM

    更新日期：2015-02-24 00:00:00

  • Localization of the Cl-/HCO3- anion exchanger binding site to the amino-terminal region of carbonic anhydrase II.

    abstract：:Human carbonic anhydrase II (CAII) possesses a binding site for an acidic motif (D887ADD) within the carboxyl-terminal region (Ct) of the human erythrocyte chloride/bicarbonate anion exchanger, AE1. In this study, the amino acid sequence comprising this AE1 binding site was localized to the first 17 residues of CAII, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0015111

    authors： Vince JW,Carlsson U,Reithmeier RA

    更新日期：2000-11-07 00:00:00

  • Mass spectrometric characterization of the human androgen receptor ligand-binding domain expressed in Escherichia coli.

    abstract：:The ligand-binding domain (LBD) of the human androgen receptor (hAR LBD), encompassing amino acids (AAs) 647-919, was expressed in Escherichia coli with an N-terminal polyhistidine tag (His(10)-hAR LBD) from a pET-16b vector. The overexpressed protein was initially insoluble in inclusion bodies, and was subsequently s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi010525j

    authors： Zhu Z,Becklin RR,Desiderio DM,Dalton JT

    更新日期：2001-09-11 00:00:00

  • Sterol orientations in phosphatidylcholine liposomes as determined by deuterium NMR.

    abstract：:Deuterium magnetic resonance spectra (55.26 MHz) of cholesterol-3 alpha-d1 and epicholesterol-3 beta-d1 in dipalmitoylglycerophosphocholine (DPPC) liposomes were measured as a function of sterol-to-phospholipid ratio below (24 degrees C) and above (60 degrees C) the phase transition temperature of DPPC. From the quadr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00353a017

    authors： Murari R,Murari MP,Baumann WJ

    更新日期：1986-03-11 00:00:00

  • Hydrolysis of monodisperse phosphatidylcholines by phospholipase A2 occurs on vessel walls and air bubbles.

    abstract：:Hydrolysis of monodisperse short chain phosphatidylcholines, far below their critical micelle concentration, by phospholipase A2 (PLA2) and other interfacial enzymes is characterized. Results show that virtually all the observed hydrolysis by pancreatic and human inflammatory PLA2 occurs on surfaces of the reaction ve...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi990194z

    authors： Yu BZ,Berg OG,Jain MK

    更新日期：1999-08-10 00:00:00

  • RNA aptamers as conformational probes and regulatory agents for plasminogen activator inhibitor-1.

    abstract：:The hallmark of serpins is the ability to undergo the so-called "stressed-to-relaxed" switch during which the surface-exposed reactive center loop (RCL) becomes incorporated as strand 4 in central beta-sheet A. RCL insertion drives not only the inhibitory reaction of serpins with their target serine proteases but also...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi100066j

    authors： Madsen JB,Dupont DM,Andersen TB,Nielsen AF,Sang L,Brix DM,Jensen JK,Broos T,Hendrickx ML,Christensen A,Kjems J,Andreasen PA

    更新日期：2010-05-18 00:00:00

  • Evolutionarily distinct versions of the multidomain enzyme α-isopropylmalate synthase share discrete mechanisms of V-type allosteric regulation.

    abstract：:Understanding the evolution of allostery in multidomain enzymes remains an important step in improving our ability to identify and exploit structure-function relationships in allosteric mechanisms. A recent protein similarity network for the DRE-TIM metallolyase superfamily indicated there are two evolutionarily disti...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi500702u

    authors： Kumar G,Frantom PA

    更新日期：2014-07-29 00:00:00

  • Inactivation of steroid sulfatase by an active site-directed inhibitor, estrone-3-O-sulfamate.

    abstract：:Steroid sulfatases are responsible for the hydrolysis of 3beta-hydroxy steroid sulfates, such as cholesterol and pregnenolone sulfate, and have an important role in regulating the synthesis of estrogenic steroids, from estrone sulfate and dehydroepiandrosterone sulfate, in endocrine-dependent tumors. Although little i...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00036a025

    authors： Purohit A,Williams GJ,Howarth NM,Potter BV,Reed MJ

    更新日期：1995-09-12 00:00:00

  • The 56-59-kilodalton protein identified in untransformed steroid receptor complexes is a unique protein that exists in cytosol in a complex with both the 70- and 90-kilodalton heat shock proteins.

    abstract：:It has previously been shown that 9S, untransformed progestin, estrogen, androgen, and glucocorticoid receptor complexes in rabbit uterine and liver cytosols contain a 59-kDa protein [Tai, P. K., Maeda, Y., Nakao, K., Wakim, N. G., Duhring, J. L., & Faber, L. E. (1986) Biochemistry 25, 5269-5275]. In this work we show...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00473a021

    authors： Sanchez ER,Faber LE,Henzel WJ,Pratt WB

    更新日期：1990-05-29 00:00:00

  • Tubulin-G protein association stabilizes GTP binding and activates GTPase: cytoskeletal participation in neuronal signal transduction.

    abstract：:It has been suggested that dimeric tubulin can participate in the signal transduction process through its association with the GTP-binding (G) proteins Gs and Gi1. Using the photoaffinity GTP analog, azidoanilido-GTP, it has been shown that the transfer of nucleotide from tubulin to G alpha s and G alpha i1 is the key...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00198a052

    authors： Roychowdhury S,Rasenick MM

    更新日期：1994-08-16 00:00:00

  • 1H NMR dipolar echo decay spectroscopy: a sensitive probe of membrane structure.

    abstract：:The structural and motional properties of membrane lipids in various physical states and macroscopic organizations are elucidated by 1H NMR dipolar echo decay spectroscopy (DECODE). Multilamellar lipid dispersions in the gel (L beta') and liquid-crystalline (L alpha) states and a nonbilayer, hexagonal (HII) phase are ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00488a027

    authors： Janes N,Rubin E,Taraschi TF

    更新日期：1990-09-11 00:00:00

  • Lipid Membranes Influence the Ability of Small Molecules To Inhibit Huntingtin Fibrillization.

    abstract：:Several diseases, including Alzheimer's disease, Parkinson's disease, and Huntington's disease (HD), are associated with specific proteins aggregating and depositing within tissues and/or cellular compartments. The aggregation of these proteins is characterized by the formation of extended, β-sheet rich fibrils, terme...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.9b00739

    authors： Beasley M,Stonebraker AR,Hasan I,Kapp KL,Liang BJ,Agarwal G,Groover S,Sedighi F,Legleiter J

    更新日期：2019-10-29 00:00:00

  • Nuclear magnetic resonance solution structure of an undecanucleotide duplex with a complementary thymidine base opposite a 10R adduct derived from trans addition of a deoxyadenosine N6-amino group to (-)-(7R,8S,9R,10S)-7,8-dihydroxy- 9,10-epoxy-7,8,9,10-t

    abstract：:The solution structure of a modified undecamer duplex containing (-)-(7R,8S,9R,10S)-7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a] pyrene covalently bonded through trans ring opening at C10 of the epoxide by the N6-amino group of deoxyadenosine (dA) was studied. This diol epoxide 1 diastereomer has the benzylic ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00028a009

    authors： Schurter EJ,Sayer JM,Oh-hara T,Yeh HJ,Yagi H,Luxon BA,Jerina DM,Gorenstein DG

    更新日期：1995-07-18 00:00:00

  • Identification of Filamin A Mechanobinding Partner I: Smoothelin Specifically Interacts with the Filamin A Mechanosensitive Domain 21.

    abstract：:Filamin A (FLNA) is a ubiquitously expressed actin cross-linking protein and a scaffold of numerous binding partners to regulate cell proliferation, migration, and survival. FLNA is a homodimer, and each subunit has an N-terminal actin-binding domain followed by 24 immunoglobulin-like repeats (R). FLNA mediates mechan...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.9b00100

    authors： Wang L,Nakamura F

    更新日期：2019-11-26 00:00:00

  • Effects of pH and iminosugar pharmacological chaperones on lysosomal glycosidase structure and stability.

    abstract：:Human lysosomal enzymes acid-beta-glucosidase (GCase) and acid-alpha-galactosidase (alpha-Gal A) hydrolyze the sphingolipids glucosyl- and globotriaosylceramide, respectively, and mutations in these enzymes lead to the lipid metabolism disorders Gaucher and Fabry disease, respectively. We have investigated the structu...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9002265

    authors： Lieberman RL,D'aquino JA,Ringe D,Petsko GA

    更新日期：2009-06-09 00:00:00

  • Exploration of the Effects of γ-Phosphate-Modified ATP Analogues on Histidine Kinase Autophosphorylation.

    abstract：:While two-component systems (TCSs), composed of a sensor histidine kinase (HK) and a response regulator, are the main signaling pathways in bacteria, global TCS activity remains poorly described. Here, we report the kinetic parameters of the HK autophosphorylation reaction using previously uncharacterized γ-phosphate-...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b00485

    authors： Chase OM,Espinasse A,Wilke KE,Carlson EE

    更新日期：2018-07-24 00:00:00

  • Characterization of RNA aptamer binding by the Wilms' tumor suppressor protein WT1.

    abstract：:The interaction of the zinc finger protein WT1 with RNA aptamers has been investigated using a quantitative binding assay, and the results have been compared to those from a previous study of the DNA binding properties of this protein. A recombinant peptide containing the four zinc fingers of WT1 (WT1-ZFP) binds to re...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi001941r

    authors： Zhai G,Iskandar M,Barilla K,Romaniuk PJ

    更新日期：2001-02-20 00:00:00

  • Assignment of spectral substructures to pigment-binding sites in higher plant light-harvesting complex LHC-II.

    abstract：:The trimeric main light-harvesting complex (LHC-II) is the only antenna complex of higher plants of which a high-resolution 3D structure has been obtained (Kühlbrandt, W., Wang, D., and Fujiyoshi, Y. (1994) Nature 367, 614-621) and which can be refolded in vitro from its components. Four different recombinant forms of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi015875k

    authors： Rogl H,Schödel R,Lokstein H,Kühlbrandt W,Schubert A

    更新日期：2002-02-19 00:00:00

  • 23,24,25-Trihydroxyvitamin D3, 24,25,26-trihydroxyvitamin D3, 24-keto-25-hydroxyvitamin D3, and 23-dehydro-25-hydroxyvitamin D3: new in vivo metabolites of vitamin D3.

    abstract：:Four new in vivo metabolites of vitamin D3 were isolated from the blood plasma of chicks given large doses of vitamin D3. The metabolites were isolated by methanol-chloroform extraction and a series of chromatographic procedures. By use of mass spectrometry, ultraviolet absorption spectrophotometry, and specific chemi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00529a010

    authors： Wichmann JK,Schnoes HK,DeLuca HF

    更新日期：1981-12-22 00:00:00

  • Misfolding of the cystic fibrosis transmembrane conductance regulator and disease.

    abstract：:Understanding the structural basis for defects in protein function that underlie protein-based genetic diseases is the fundamental requirement for development of therapies. This situation is epitomized by the cystic fibrosis transmembrane conductance regulator (CFTR)-the gene product known to be defective in CF patien...

    journal_title：Biochemistry

    pub_type： 杂志文章,评审

    doi：10.1021/bi702209s

    authors： Cheung JC,Deber CM

    更新日期：2008-02-12 00:00:00

  • Dissection of porphyrin-induced conformational dynamics in the heme biosynthesis enzyme ferrochelatase.

    abstract：:Human ferrochelatase (EC 4.99.1.1) catalyzes the insertion ferrous iron into protoporphyrin IX as the last step in heme biosynthesis, an essential process to most organisms given the vast intracellular functions of heme. Even with multiple ferrochelatase structures available, the exact mechanism for iron insertion int...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi300704c

    authors： Asuru AP,An M,Busenlehner LS

    更新日期：2012-09-11 00:00:00

  • Characterization of the N-terminal and C-terminal domains of human apolipoprotein(a): relevance to fibrin binding.

    abstract：:The structural domains of human apolipoprotein(a) (apo(a)) in the lipoprotein(a) (Lp(a)) particle have been recently investigated by limited proteolysis [Huby, T., Doucet, C., Dieplinger, H., Chapman, J., & Thillet, J. (1994) Biochemistry 33, 3335-3341]. We have shown that apo(a) can be cleaved into two structural dom...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00022a011

    authors： Huby T,Schröder W,Doucet C,Chapman J,Thillet J

    更新日期：1995-06-06 00:00:00

  • Structural characterization of lactoperoxidase in the heme environment by proton NMR spectroscopy.

    abstract：:The heme environmental structures of lactoperoxidase (LP) have been studied by the use of hyperfine-shifted proton NMR and optical absorption spectra. The NMR spectra of the enzyme in native and cyanide forms in H2O indicated that the fifth ligand of the heme iron is the histidyl imidazole with an anionic character an...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00368a002

    authors： Shiro Y,Morishima I

    更新日期：1986-10-07 00:00:00

  • Evolution of enzymatic activities in the enolase superfamily: L-talarate/galactarate dehydratase from Salmonella typhimurium LT2.

    abstract：:We assigned l-talarate dehydratase (TalrD) and galactarate dehydratase (GalrD) functions to a group of orthologous proteins in the mechanistically diverse enolase superfamily, focusing our characterization on the protein encoded by the Salmonella typhimurium LT2 genome (GI:16766982; STM3697). Like the homologous mande...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi7008882

    authors： Yew WS,Fedorov AA,Fedorov EV,Almo SC,Gerlt JA

    更新日期：2007-08-21 00:00:00