The newly discovered Parkinson's disease associated Finnish mutation (A53E) attenuates α-synuclein aggregation and membrane binding.

abstract：:Gap junctions, transmembrane protein channels that directly connect the cytoplasm of neighboring cells and enable the exchange of molecules between cells, are a promising new frontier for therapeutic delivery. Specifically, cell-derived lipid vesicles that contain functional gap junction channels, termed Connectosomes...

journal_title：Biochemistry

authors： Gadok AK,Zhao C,Meriwether AI,Ferrati S,Rowley TG,Zoldan J,Smyth HDC,Stachowiak JC

更新日期：2018-01-09 00:00:00

abstract：:The accessibility of the asparagine-linked carbohydrate chains of human thyrotropin (hTSH) and free alpha and beta subunits was investigated by their susceptibility to endoglycosidases H and F as well as to peptide:N-glycosidase F. Iodinated hTSH or subunits were incubated with a commercial enzyme preparation containi...

journal_title：Biochemistry

authors： Ronin C,Papandreou MJ,Canonne C,Weintraub BD

更新日期：1987-09-08 00:00:00

abstract：:The phosphorelay signal transduction pathway controls sporulation initiation in Bacillus subtilis. Transfer of a phosphoryl group from multiple kinases (KinA and KinB) through a single domain response regulator homologue (Spo0F), a phosphotransferase (Spo0B), and ultimately to a transcriptional regulator, (Spo0A) acti...

journal_title：Biochemistry

authors： Tzeng YL,Feher VA,Cavanagh J,Perego M,Hoch JA

更新日期：1998-11-24 00:00:00

abstract：:The HAMP domain is a conserved motif widely distributed in prokaryotic and lower eukaryotic organisms, where it is often found in transmembrane receptors that regulate two-component signaling pathways. The motif links receptor input and output modules and is essential to receptor structure and signal transduction. Rec...

journal_title：Biochemistry

authors： Swain KE,Falke JJ

更新日期：2007-12-04 00:00:00

abstract：:An improved purification procedure for the alpha 2 beta 2 complex of tryptophan synthase from Escherichia coli has been developed. It consists of DEAE-Sephacel chromatography, followed by hydrophobic chromatography on Sepharose CL 4B, and leads to material with a higher specific activity than reported previously. Inhi...

journal_title：Biochemistry

authors： Tschopp J,Kirschner K

更新日期：1980-09-16 00:00:00

abstract：:Nuclear magnetic resonance (NMR) has been used to monitor the conformation and dynamics of the d(C1-G2-A3-T4-T5-A6-T6-A5-A4-T3-C2-G1) self-complementary dodecanucleotide duplex (henceforth called Pribnow 12-mer), which contains a TATAAT Pribnow box and a central core of eight dA X dT base pairs. The exchangeable imino...

journal_title：Biochemistry

authors： Patel DJ,Kozlowski SA,Weiss M,Bhatt R

更新日期：1985-02-12 00:00:00

abstract：:A synthetic gene coding for the inhibitor protein of bovine heart mitochondrial F1 adenosine triphosphatase was designed and cloned in Escherichia coli. Recombinant F1-ATPase inhibitor protein was overproduced in E. coli and secreted to the periplasmic space. Biologically active recombinant F1-ATPase inhibitor protein...

journal_title：Biochemistry

authors： Van Heeke G,Deforce L,Schnizer RA,Shaw R,Couton JM,Shaw G,Song PS,Schuster SM

更新日期：1993-09-28 00:00:00

abstract：:Evidence is presented that indicates tropoelastin is derived from a soluble elastin with a molecular weight of 95000. Tropoelastin and its proposed precursor were isolated from the aortas of copper-deficient chicks. Although it is doubtful that the proposed precursor is an initial product of elastin translation, i.e.,...

journal_title：Biochemistry

authors： Rucker RB,Heng-Khoo CS,Dubick M,Lefevre M,Cross CE

更新日期：1979-09-04 00:00:00

abstract：:The interaction of nile red (NR) with apomyoglobin (ApoMb) in the native (pH 7) and molten globule (pH 4) states was investigated using experimental and computational methods. NR binds to hydrophobic locations in ApoMb with higher affinity (K(d) = 25 +/- 5 microM) in the native state than in the molten globule state (...

journal_title：Biochemistry

authors： Polverini E,Cugini G,Annoni F,Abbruzzetti S,Viappiani C,Gensch T

更新日期：2006-04-25 00:00:00

abstract：:8-OxoGua (8-oxo-7,8-dihydroguanine) is produced in nucleic acids as well as in nucleotide pools of cells, by reactive oxygen species normally formed during cellular metabolic processes. MutT protein of Escherichia coli specifically degrades 8-oxoGua-containing deoxyribo- and ribonucleoside triphosphates to correspondi...

journal_title：Biochemistry

authors： Ito R,Hayakawa H,Sekiguchi M,Ishibashi T

更新日期：2005-05-03 00:00:00

abstract：:The reactions of fully reduced and mixed-valence bovine heart cytochrome c oxidase with dioxygen have been reinvestigated in the absence and presence of metal ions (Zn(2+), Ni(2+), and Cd(2+)) by time-resolved optical absorption spectroscopy using the CO flow-flash technique. The time-resolved data were recorded on a ...

journal_title：Biochemistry

authors： Szundi I,Cappuccio J,Einarsdóttir O

更新日期：2004-12-21 00:00:00

abstract：:Kinetic parameters for triosephosphate isomerase catalysis of the elimination reaction of an equilibrium mixture of dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (DGAP) to form methylglyoxal and phosphate ion are reported for the enzyme from rabbit muscle. Pseudo-first-order rate constants for the...

journal_title：Biochemistry

authors： Richard JP

更新日期：1991-05-07 00:00:00

abstract：:Two novel heme-containing peroxidases, one able to incorporate halogens into aromatic substrates and the other able to remove them, have recently been isolated from marine sources and initially characterized by Chen et al. [(1991) J. Biol. Chem. 266, 23909-23915; (1996) J. Biol. Chem. 271, 4609-4612]. The haloperoxida...

journal_title：Biochemistry

authors： Roach MP,Chen YP,Woodin SA,Lincoln DE,Lovell CR,Dawson JH

更新日期：1997-02-25 00:00:00

abstract：:Previous studies of recombinant full-length human apolipoprotein A-V (apoA-V) provided evidence of the presence of two independently folded structural domains. Computer-assisted sequence analysis and limited proteolysis studies identified an N-terminal fragment as a candidate for one of the domains. C-Terminal truncat...

journal_title：Biochemistry

authors： Wong K,Beckstead JA,Lee D,Weers PM,Guigard E,Kay CM,Ryan RO

更新日期：2008-08-19 00:00:00

abstract：:Sin3 forms the scaffold for a multiprotein corepressor complex that silences transcription via the action of histone deacetylases. Sin3 is recruited to the DNA by several DNA binding repressors, such as the helix-loop-helix proteins of the Mad family. Here, we elaborate on the Mad-Sin3 interaction based on a binding s...

journal_title：Biochemistry

authors： van Ingen H,Lasonder E,Jansen JF,Kaan AM,Spronk CA,Stunnenberg HG,Vuister GW

更新日期：2004-01-13 00:00:00

abstract：:Both agonist- and antagonist-bound glucocorticoid receptors (GRs) and progesterone receptors (PRs) regulate gene transcription with the assistance of corepressors (NCoR and SMRT) and coactivators (TIF2/GRIP1, SRC1, and AIB1). Receptor binding of these cofactors is competitive and is considered to involve interactions ...

journal_title：Biochemistry

authors： Wang D,Wang Q,Awasthi S,Simons SS Jr

更新日期：2007-07-10 00:00:00

abstract：:To elucidate the role of the proline residue in the engineered signal sequence that directs the secretion of human lysozyme in Saccharomyces cerevisiae, we have remodeled an idealized signal sequence L8 = Met-Arg-(Leu)8-Pro-Leu-Ala-Ala-Leu-Gly [Yamamoto, Y., Taniyama, Y., Kikuchi, M., & Ikehara, M. (1987) Biochem. Bio...

journal_title：Biochemistry

authors： Yamamoto Y,Taniyama Y,Kikuchi M

更新日期：1989-03-21 00:00:00

abstract：:Several experimental approaches have been employed to independently determine rates of dissociation for Escherichia coli RNA polymerase from the T7 bacteriophage A1, A2, A3, and D promoters. Heparin challenge measurements employing abortive initiation turnover rates as an index of promoter occupancy were carried out o...

journal_title：Biochemistry

authors： Cech CL,McClure WR

更新日期：1980-05-27 00:00:00

abstract：:The purified ATPase of sarcoplasmic reticulum was covalently labeled with N-iodoacetyl-N'-(5-sulfo-1-naphthyl)ethylenediamine (1,5-IAEDANS) or with iodoacetamidofluorescein (IAF). In reconstituted vesicles containing both types of ATPase molecules fluorescence energy transfer was observed from the IAEDANS (donor) to t...

journal_title：Biochemistry

authors： Vanderkooi JM,Ierokomas A,Nakamura H,Martonosi A

更新日期：1977-04-05 00:00:00

abstract：:Stopped-flow measurements have been employed to study the kinetics of the conformational changes in TetR (B) induced by tetracycline binding with and without Mg(2+) ions. Result of stopped-flow fluorometry measurements at pH 8.0 indicate conformational changes in the helix-turn-helix motif in the N-terminal domain and...

journal_title：Biochemistry

authors： Kedracka-Krok S,Gorecki A,Bonarek P,Wasylewski Z

更新日期：2005-01-25 00:00:00

abstract：:The role of acetylation on the conformation and association state of the inner core histone octamer isolated from HeLa cells was examined. Preparation of suitable quantities of pure acetylated and control inner core histones from HeLa cells required the development of a new preparative procedure. The results from size...

journal_title：Biochemistry

authors： Prevelige PE Jr,Fasman GD

更新日期：1987-05-19 00:00:00

abstract：:Incubation of bovine brain derived acidic fibroblast growth factor (aFGF) with bovine or human thrombin, 0.5 NIH unit/mL, for 24 h at 37 degrees C results in cleavage of the mitogen, generating a 14-kilodalton fragment which has significantly reduced affinity for immobilized heparin as compared to aFGF, and is at leas...

journal_title：Biochemistry

authors： Lobb RR

更新日期：1988-04-05 00:00:00

abstract：:Understanding the mechanisms of free energy transfer in metabolism is fundamental to understanding how the chemical forces that sustain the molecular organization of the cell are distributed. Recent studies of molecular motors (1-3) and ATP-driven proton transport (4-6) describe how chemical potential is transferred a...

journal_title：Biochemistry

authors： Wei J,Tang QX,Varlamova O,Roche C,Lee R,Leyh TS

更新日期：2002-07-02 00:00:00

abstract：:The dimerization specificity of the recombinantly expressed and purified rod domain of adult and neonatal chicken myosin heavy chain was analyzed using metal chelation chromatography. Our results indicate that full-length adult and neonatal rods preferentially formed homodimers when renatured from an equimolar mixture...

journal_title：Biochemistry

authors： Singh S,Bandman E

更新日期：2006-04-18 00:00:00

abstract：:Messenger RNA degradation is an important component of overall gene expression. During the final step of eukaryotic mRNA degradation, exoribonuclease 1 (Xrn1) carries out 5' → 3' processive, hydrolytic degradation of RNA molecules using divalent metal ion catalysis. To initiate studies of the 5' → 3' RNA decay machine...

journal_title：Biochemistry

authors： Langeberg CJ,Welch WRW,McGuire JV,Ashby A,Jackson AD,Chapman EG

更新日期：2020-04-21 00:00:00

abstract：:Modification of pig kidney fructose-1,6-bisphosphatase with 2,3-butanedione in borate buffer (pH 7.8) leads to the loss of the activation of the enzyme by monovalent cations, as well as to the loss of allosteric adenosine 5'-monophosphate (AMP) inhibition. In agreement with the results obtained for the butanedione mod...

journal_title：Biochemistry

authors： Marcus F

更新日期：1976-08-10 00:00:00

abstract：:The nuclear transport of the chromatin remodeling protein nucleoplasmin and chromatin building histones is mediated by importins. Nucleoplasmin (NP) contains a classical bipartite nuclear localization signal (NLS) that is recognized by the importin α/β heterodimer, while histones present multiple NLS-like motifs that ...

journal_title：Biochemistry

authors： Arregi I,Falces J,Bañuelos S,Urbaneja MA,Taneva SG

更新日期：2011-08-23 00:00:00

abstract：:Carboxyl ester lipase (CEL; EC 3.1.1.13) hydrolyzes cholesteryl esters and retinyl esters in vitro. In vivo, pancreatic CEL is thought to liberate cholesterol and retinol from their esters prior to absorption in the intestine. CEL is also a major lipase in the breast milk of many mammals, including humans and mice, an...

journal_title：Biochemistry

authors： Weng W,Li L,van Bennekum AM,Potter SH,Harrison EH,Blaner WS,Breslow JL,Fisher EA

更新日期：1999-03-30 00:00:00

abstract：:The diversity, abundance, and DNA sequence representation of poly(adenylic acid) containing RNA derived from cells of Achlya ambisexualis cultured in defined and undefined media have been determined. The kinetics of hybridization of polyadenylated RNA with complementary DNA were the same for both culture conditions an...

journal_title：Biochemistry

authors： Rozek CE,Orr WC,Timberlake WE

更新日期：1978-02-21 00:00:00

abstract：:Resonance Raman spectroscopy has been used to study milk xanthine oxidase, an enzyme containing molybdenum, binuclear iron-sulfur clusters, and FAD as cofactors. The contribution of FAD dominates the resonance Raman spectrum at frequencies above 500 cm-1. As expected, no bands assignable to FAD are observed in deflavo...

journal_title：Biochemistry

authors： Willis LJ,Loehr TM

更新日期：1985-05-21 00:00:00