Abstract:
:The phosphorelay signal transduction pathway controls sporulation initiation in Bacillus subtilis. Transfer of a phosphoryl group from multiple kinases (KinA and KinB) through a single domain response regulator homologue (Spo0F), a phosphotransferase (Spo0B), and ultimately to a transcriptional regulator, (Spo0A) activates sporulation. Counteracting this response are phosphatases (RapA and RapB), which can short-circuit this phosphorelay via dephosphorylation of Spo0F. In vitro assays of RapB activity on phosphorylated Spo0F alanine-scanning mutants have been used to identify Spo0F residues critical for interactions between these proteins. The Spo0F surface comprised of the beta1-alpha1 loop and N-terminal half of helix alpha1 has the largest number of residues in which an alanine substitution leads to resistance or decreased sensitivity to RapB phosphatase activity. Other mutations desensitizing Spo0F to RapB are also located near the site of phosphorylation on the beta3-alpha3 and beta4-alpha4 loops. This surface is similar to but not the same as the surface identified for KinA and Spo0B interactions with Spo0F. Divalent metal ions were shown to be required for RapB activity, and this activity was insensitive to vanadate, suggesting that Rap phosphatases catalyze acyl phosphate hydrolysis by inducing conformational changes in phosphorylated Spo0F, which results in increased autodephosphorylation. Arginine 16 of Spo0F is proposed to play a role in catalysis, and similarities between the mechanisms for RapB catalyzed Spo0F approximately P hydrolysis and GAP (GTPase activating protein)-assisted GTP hydrolysis of Ras are discussed.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Tzeng YL,Feher VA,Cavanagh J,Perego M,Hoch JAdoi
10.1021/bi981340osubject
Has Abstractpub_date
1998-11-24 00:00:00pages
16538-45issue
47eissn
0006-2960issn
1520-4995pii
bi981340ojournal_volume
37pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The binding of rabbit globin mRNA to the 25-kDa cap binding protein eIF-4E from human erythrocytes was found to be 5.3-fold stronger than the binding of the cap analogue m7GpppG to eIF-4E [Gross et al. (1990) Biochemistry 29, 5008-5012]. In order to investigate whether this effect is due to the longer sequence of nucl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00120a020
更新日期:1992-02-11 00:00:00
abstract::A new type of thrombin exo-site inhibitor has been designed with enhanced inhibitory potency and increased metabolic stability. With the aid of the model of the structure of the thrombin-hirudin fragment complex [Yue, S.-Y., DiMaio, J., Szewczuk, Z., Purisima, E. O., Ni, F., & Konishi, Y. (1992) Protein Eng. 5, 77-85]...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00153a004
更新日期:1992-09-29 00:00:00
abstract::The enzyme endonuclease V initiates repair of deaminated DNA bases by making an endonucleolytic incision on the 3' side one nucleotide from a base lesion. In this study, we have used site-directed mutagenesis to characterize the role of the highly conserved residues D43, E89, D110, and H214 in Thermotoga maritima endo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060512b
更新日期:2006-08-29 00:00:00
abstract::A systematic 220-MHz proton nuclear magnetic resonance (nmr) study has been made of all common purine and pyrimidine 2'(3')-ribonucleotides in D2O solutions at 20 plus or minus 2 degrees. Spectra for the entire series were measured under similar conditions of concentration, temperature, and ionic strength, thereby fac...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00674a013
更新日期:1975-02-11 00:00:00
abstract::X-ray reflectivity was used to study the interaction of the PX domain of p40(phox) protein (p40(phox)-PX) with a Langmuir monolayer of a mixture of SOPC (1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine), SOPS (1-stearoyl-2-oleoyl-sn-glycero-3-phosphoserine), and DPPtdIns(3)P (1,2-dipalmitoylphosphatidylinositol 3-phos...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061133l
更新日期:2006-11-14 00:00:00
abstract::A wide variety of biological processes including differentiation, regeneration, and cancer progression are regulated by shedding of membrane-anchored proteins. One of the major sheddases is A Disintegrin And Metalloprotease-17 (ADAM17) whose extracellular region consists of a pro-, a catalytic, a disintegrin-, and a m...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00497
更新日期:2015-09-29 00:00:00
abstract::1-Bromovinyl (I), Z-2-bromovinyl (II), 1,2-dibromoethyl (III), and a series of 4-(halomethyl)-2-nitrophenyl (IVa-c) diethyl phosphate esters were examined as substrates and mechanism-based inhibitors for the bacterial phosphotriesterase. All of these compounds were found to act as substrates for the enzyme. Inhibitor ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi970680r
更新日期:1997-07-22 00:00:00
abstract::Copper binding to the human copper chaperone for superoxide dismutase (hCCS) has been investigated by X-ray absorption spectroscopy. Stoichiometry measurements on the dialyzed, as-isolated protein indicated that up to 3.5 Cu ions bound per hCCS molecule. Reduction with either sodium dithionite or dithiothreitol decrea...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi000690j
更新日期:2000-06-27 00:00:00
abstract::When grown under O2-limited conditions, Escherichia coli expresses a cytochrome bd quinol oxidase that has an unusually high affinity for O2. We have studied the reaction of cytochrome bd with CO and O2 by rapid-reaction spectrophotometry. The reduced enzyme forms a photosensitive ferrocytochrome d-CO complex, and fol...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00254a021
更新日期:1994-12-20 00:00:00
abstract::Despite many studies suggesting the role of enolpyruvate as a bound intermediate in the pyruvate kinase reaction, direct evidence for it has been lacking. By use of a combination of chemical trapping and isolation of a derivative, significant amounts of enzyme-bound enolpyruvate have now been demonstrated. The method ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00217a022
更新日期:1991-01-22 00:00:00
abstract::The cystic fibrosis transmembrane conductance regulator forms an anion-selective channel. We previously showed that charge selectivity, the ability to discriminate between anions and cations, occurs near the cytoplasmic end of the channel. The molecular determinants of charge selectivity, however, are unknown. We inve...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990155n
更新日期:1999-04-27 00:00:00
abstract::The interaction of lipoprotein lipase (LpL) and a nonhydrolyzable phosphatidylcholine, 1,2-ditetradecyl-rac-glycero-3-phosphocholine (C14-ether-PC), has been studied by several physical methods. Analysis of the circular dichroic spectrum of LpL gave the following fractional conformation: 35% alpha-helix, 30% beta-plea...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00352a020
更新日期:1986-02-25 00:00:00
abstract::In order to define structural regions in thrombin that interact with hirudin, the N alpha-dinitrofluorobenzyl analogue of an undecapeptide was synthesized corresponding to residues 54-64 of hirudin [GDFEEIPEEY(O35SO3)L (DNFB-[35S]Hir54-64)]. DNFB-[35S]Hir54-64 was reacted at a 10-fold molar excess with human alpha-thr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00479a006
更新日期:1990-07-10 00:00:00
abstract::Combined quantum mechanical/molecular mechanical (QM/MM) simulations are carried out to analyze factors that dictate the proton transfer in carbonic anhydrase II (CAII), an enzyme that has been used as a prototypical example of long-range proton transfers in biomolecules. In contrast to the long-held conjecture in the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701950j
更新日期:2008-02-26 00:00:00
abstract::The interactions of two plant annexins, annexin 24(Ca32) from Capsicum annuum and annexin Gh1 from Gossypium hirsutum, with phospholipid membranes have been characterized using liposome-based assays and adsorption to monolayers. These two plant annexins show a preference for phosphatidylserine-containing membranes and...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0516226
更新日期:2005-12-13 00:00:00
abstract::One of the most intriguing questions confronting the bone morphogenetic protein family is the mechanism of ligand recognition, because there are more ligands than receptors. Crystal structures of two type II receptors, ActR-II and BMPR-II, are essentially identical, and a loop structure (A-loop) has been suggested to ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi3003023
更新日期:2012-09-04 00:00:00
abstract::Proton and phosphorus-31 nuclear magnetic resonance (1H and 31P NMR) studies of the interaction between a tridecapeptide pheromone, the alpha-factor of Saccharomyces cerevisiae, and sonicated lipid vesicles are reported. 31P NMR studies demonstrate that there is interaction of the peptide with the phosphorus headgroup...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00436a017
更新日期:1989-05-16 00:00:00
abstract::To identify the specific component(s) in the target membrane involved in fusion of vesicular stomatitis virus (VSV), we examined the interaction of the virus with human erythrocyte membranes with asymmetric and symmetric bilayer distributions of phospholipids. Fusion was monitored spectrofluorometrically by the octade...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00469a005
更新日期:1990-05-01 00:00:00
abstract::The present study was undertaken for the purpose of evaluating the solvent perturbation technique as a method to identify enzyme catalytic residues. For establishment of expected directions and sizes of pKa perturbations for different types of acids in different classes of solvents, a study of the pKa of a series of a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00287a013
更新日期:1983-08-30 00:00:00
abstract::The influenza M2 protein is the target of the amantadine family of antiviral drugs, and its transmembrane (TM) domain structure and dynamics have been extensively studied. However, little is known about the structure of the highly conserved N-terminal ectodomain, which contains epitopes targeted by influenza vaccines....
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00727
更新日期:2016-09-27 00:00:00
abstract::A common motif in protein structures is the assembly of alpha-helices. Natural alpha-helical assemblies, such as helical bundles and coiled coils, consist of multiple right-handed alpha-helices. Here we design a protein complex containing both left-handed and right-handed helices, with peptides of D- and L-amino acids...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi010725v
更新日期:2001-07-31 00:00:00
abstract::Earlier thermodynamic studies of the intermolecular interactions between mature oxytocin and neurophysin, and of the effects of these interactions on neurophysin folding, raised questions about the intramolecular interactions of oxytocin with neurophysin within their common precursor. To address this issue, the disulf...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9912950
更新日期:1999-10-12 00:00:00
abstract::We have determined the formal potential values for each electron transfer to electron transfer flavoprotein-ubiquinone oxidoreductase (ETF-QO), in order to further characterize the thermodynamics of electron transport from various acyl-CoA thioesters to the mitochondrial ubiquinone pool. ETF-QO contains one [4Fe-4S]2+...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00162a012
更新日期:1992-12-01 00:00:00
abstract::A structural understanding of the nature and scope of serpin inhibition mechanisms has been limited by the inability so far to crystallize any serpin-proteinase complex. We describe here the application of [(1)H-(15)N]-HSQC NMR on uniformly and residue-selectively (15)N-labeled serpin alpha(1)-proteinase inhibitor (Pi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001152+
更新日期:2000-10-03 00:00:00
abstract::The arrangement of the mRNA on the Escherichia coli ribosome with respect to ribosomal RNA sites has been investigated by photochemical cross-linking experiments. mRNA analogues 51-54 nucleotides in length contained a Shine-Dalgarno sequence, a single codon for tRNA(Gly), and 4-thiouridine (s4U) in the 5' third of the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00176a040
更新日期:1994-03-15 00:00:00
abstract::Spectrophotometric titration of pyridoxamine-pyruvate transaminase (EC 2.6.1.30) with pyridoxal at pH 7.15 gives four equivalent binding sites per tetramer. The pH dependence of the equilibrium constant for the association of 5'-deoxypyridoxal with the active site lysine residue was determined spectrophotometrically. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00643a013
更新日期:1977-11-29 00:00:00
abstract::A highly active, large-scale preparation of ubiquinol:cytochrome c2 oxidoreductase (EC 1.10.2.2; cytochrome bc1 complex) has been obtained from Rhodobacter sphaeroides. The enzyme was solubilized from chromatophores by using dodecyl maltoside in the presence of glycerol and was purified by anion-exchange and gel filtr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00463a004
更新日期:1990-03-20 00:00:00
abstract::The mechanism by which the contractile machinery of muscle is assembled and maintained is not well-understood. Members of the cysteine-rich protein (CRP) family have been implicated in these processes. Three vertebrate CRPs (CRP1-3) that exhibit developmentally regulated muscle-specific expression have been identified...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi982036y
更新日期:1999-05-04 00:00:00
abstract::Paracoccus denitrificans synthesizes an inducible, periplasmic, blue copper protein [Husain, M., & Davidson, V.L. (1985) J. Biol. Chem. 260, 14626-14629] that can be classified as an amicyanin on the basis of its ability to accept electrons from methylamine dehydrogenase. The amino acid composition and sequence of the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00357a020
更新日期:1986-05-06 00:00:00
abstract::1,4-Dihydroxy-2-naphthoyl-coenzyme A (DHNA-CoA) synthase, or MenB, catalyzes an intramolecular Claisen condensation involving two oxyanion intermediates in the biosynthetic pathway of menaquinone, an essential respiration electron transporter in many microorganisms. Here we report the finding that the DHNA-CoA product...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200376x
更新日期:2011-07-05 00:00:00