Abstract：
:To identify the specific component(s) in the target membrane involved in fusion of vesicular stomatitis virus (VSV), we examined the interaction of the virus with human erythrocyte membranes with asymmetric and symmetric bilayer distributions of phospholipids. Fusion was monitored spectrofluorometrically by the octadecylrhodamine dequenching assay. Fusion of VSV with lipid-symmetric erythrocyte ghosts was rapid at 37 degrees C and low pH, whereas little or no fusion was observed with lipid-asymmetric ghosts. Conversion of phosphatidylserine in the lipid-symmetric ghost membrane to phosphatidylethanolamine by means of the enzyme phosphatidylserine decarboxylase did not alter the target membrane's susceptibility to VSV fusion. Spin-labeled phospholipid analogues with phosphatidylserine, phosphatidylethanolamine, and phosphatidylcholine headgroups incorporated into the outer leaflet of lipid-asymmetric erythrocytes did not render those membranes fusogenic. Electron spin resonance spectra showed an increased mobility of a phosphatidylcholine spin-label incorporated into the outer leaflet of lipid-symmetric erythrocyte ghosts as compared to that of lipid-asymmetric ghosts. These results indicate that the susceptibility to VSV fusion is not dependent on any particular phospholipid but rather is related to packing characteristics of the target membrane.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Herrmann A,Clague MJ,Puri A,Morris SJ,Blumenthal R,Grimaldi Sdoi
10.1021/bi00469a005subject
Has Abstractpub_date
1990-05-01 00:00:00pages
4054-8issue
17eissn
0006-2960issn
1520-4995journal_volume
29pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:It had been reported previously that the beta2 subunit of Escherichia coli tryptophan synthetase [L-serinehydrolyase (adding indole) EC 4.2.1.20] can be cleaved by trypsin into a nearly functional dimeric protein, the monomer of which consists of two large, nonoverlapping, polypeptide fragments. In the present paper, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00637a012
更新日期：1977-09-06 00:00:00
abstract：:Oxygen and nitroaromatic compounds are known to enhance the sensitivity of cells to ionizing radiation. Employing calf thymus DNA and oligo(dA)12/poly(dT), we have examined the differences to DNA damage, in particular thymine glycols and the 3'-DNA termini at strand breaks, arising from irradiation under anoxic and ox...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00060a009
更新日期：1993-03-09 00:00:00
abstract：:Amyloid nucleation through agitation was studied with beta2-microglobulin, which is responsible for dialysis-related amyloidosis, in the presence of salt under acid and neutral pH conditions. First, the aggregation of beta2-microglobulin in NaCl solutions was achieved by mildly agitating for 24 h at 37 degrees C prote...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi701968g
更新日期：2008-02-26 00:00:00
abstract：:Ner repressors of the transposable phages Mu and D108 play a central role in regulating the expression of the early (transposase) operon and in ensuring that phage growth proceeds along a lytic pathway. The latter function is analogous to that performed by the Cro protein of phage lambda. Unlike lambda Cro, however, t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00201a018
更新日期：1994-09-06 00:00:00
abstract：:The Ogg1 protein of Saccharomyces cerevisiae belongs to a family of DNA glycosylases and apurinic/apyrimidinic site (AP) lyases, the signature of which is the alpha-helix-hairpin-alpha-helix-Gly/Pro-Asp (HhH-GPD) active site motif together with a conserved catalytic lysine residue, to which we refer as the HhH-GPD/K f...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi992262n
更新日期：2000-02-22 00:00:00
abstract：:Papain-solubilized human class II (HLA-DR) antigens have been purified from cadaveric spleens by ion-exchange chromatography, gel chromatography, and immunosorbent purification. The isolated papain-solubilized antigens comprised two subunits with apparent molecular weights of 23 000 and 30 000, respectively. The circu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00264a036
更新日期：1982-10-12 00:00:00
abstract：:Ultraviolet thermal denaturation studies substantiate our earlier hypothesis that substitution of a L-nucleotide residue for a D-nucleotide within a DNA duplex permits a stable structure in which all bases are paired through Watson-Crick hydrogen bonds (Damha, M. J., Giannaris, P. A., Marfey, P., & Reid, L. S. (1991) ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00191a015
更新日期：1994-06-28 00:00:00
abstract：:We have investigated the kinetic and thermodynamic properties of carbon monoxide binding to the fully reduced quinol oxidase (cytochrome aa(3)) from the hyperthermophilic archaeon Acidianus ambivalens. After flash photolysis of CO from heme a(3), the complex recombines with an apparent rate constant of approximately 3...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990473m
更新日期：1999-08-03 00:00:00
abstract：:A major challenge to the study of protein folding is the fact that intermediate states along the reaction pathway are generally unstable and thus difficult to observe. Recently developed NMR relaxation dispersion experiments present an avenue to accessing such states, providing kinetic, thermodynamic, and structural i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051771o
更新日期：2005-11-29 00:00:00
abstract：:An NMR and nuclear Overhauser effect (NOE) analysis of Thermus thermophilus tRNAIle1a is presented. This species contains modifications including s2T54 and s4U8 [Horie, N., Hara-Yokoyama, M., Yokoyama, S., Watanabe, K., Kuchino, Y., Nishimura, S., & Miyazawa, T. (1985) Biochemistry 24, 5711-5715]. All the expected sec...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00355a010
更新日期：1986-04-08 00:00:00
abstract：:The bacterial DNA cytosine methyltransferase M.HhaI sequence-specifically modifies DNA in an S-adenosylmethionine dependent reaction. The enzyme stabilizes the target cytosine (GCGC) into an extrahelical position, with a concomitant large movement of an active site loop involving residues 80-99. We used multidimension...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi602662e
更新日期：2007-06-19 00:00:00
abstract：:Thermal perturbation of the dicluster ferredoxin from Acidianus ambivalens was investigated employing a toolbox of spectroscopic methods. FTIR and visible CD were used for assessing changes of the secondary structure and coarse alterations of the [3Fe4S] and [4Fe4S] cluster moieties, respectively. Fine details of the ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700967g
更新日期：2007-09-18 00:00:00
abstract：:The stereochemical course of the phospho group transfer catalyzed by mutant (S102C) alkaline phosphatase from Escherichia coli was investigated by using 31P nuclear magnetic resonance spectroscopy. Transphosphorylation from 4-nitrophenyl (Rp)-[16O, 17O, 18O]phosphate to (S)-propane-1,2-diol occurs with overall retenti...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00413a029
更新日期：1988-06-28 00:00:00
abstract：:Electron paramagnetic resonance studies have indicated that nitrosodisulfonate binds to pig heart citrate synthase. Titration of the enzyme with nitrosodisulfonate revealed several binding sites for the probe per subunit with one site (KD approximately 0.1 mM) having a greater affinity than the others. The substrate, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00584a028
更新日期：1979-08-21 00:00:00
abstract：:Stabilization of the plasma membrane Ca-ATPase (PMCA) in an inactive conformation upon oxidation of multiple methionines in the calcium regulatory protein calmodulin (CaM) is part of an adaptive cellular response to minimize ATP utilization and the generation of reactive oxygen species (ROS) under conditions of oxidat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0474113
更新日期：2005-03-29 00:00:00
abstract：:The cytochrome bc(1) complex from Paracoccus denitrificans and soluble fragments of its cytochrome c(1) and Rieske ISP subunits are characterized by a combined approach of protein electrochemistry and FTIR difference spectroscopy. The FTIR difference spectra provide information about alterations in the protein upon re...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi035103j
更新日期：2003-10-28 00:00:00
abstract：:Electrostatic interactions play important roles in the catalysis of chorismate to prephenate by chorismate mutase. Mutation of Gln88 to glutamate in the monofunctional chorismate mutase from Escherichia coli results in an enzyme with a pH profile of activity significantly different from that of the wild type protein. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980217u
更新日期：1998-06-23 00:00:00
abstract：:In this communication, we present data which describe optimum conditions for reverse transcription of large ribonucleic acid (RNA) templates into deoxyribonucleic acid (DNA) transcripts by the avian retrovirus reverse transcriptase in vitro. In contrast to previous studies, we have optimized all of the reaction compon...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00544a019
更新日期：1980-02-05 00:00:00
abstract：:Formation of protein-protein assemblies is essential in maintaining cell structure and function. Conservation of structural motifs and binding sites is the result of evolutionary pressure for solutions compatible with both molecular economy and regulation. PDZ domains are a typical example: A conserved fold governs sp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi061869i
更新日期：2007-01-30 00:00:00
abstract：:Homodinuclear (Pt,Pt) and heterodinuclear (Ru,Pt) metal compounds having the generalized formula M(a)NH2(CH)4NH2M(b) are shown to form specific DNA lesions which can efficiently cross-link proteins to DNA. In this study, the homodinuclear case is represented by M(a) = M(b) = [cis-Pt(Cl2)-(NH3)] and the heterodinuclear...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00095a007
更新日期：1993-11-09 00:00:00
abstract：:Monoclonal antibodies were raised against a mono-p-nitrophenyl phosphonate ester to elicit catalytic antibodies capable of hydrolyzing the analogous p-nitrophenyl ester or carbonate. Potential catalytic antibody producing clones were selected, by use of a competitive inhibition assay, on the basis of their affinity fo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00494a023
更新日期：1990-10-23 00:00:00
abstract：:The functionality of the membrane-bound, ubiquinone-dependent pyruvate oxidase from the respiratory chain of Escherichia coli was reconstituted with a supported lipidic structure. The artificial structure was especially designed to allow the electrochemical control of the quinone pool through the lateral mobility of t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002325y
更新日期：2001-02-06 00:00:00
abstract：:The active site of methionyl-tRNA synthetase (MetRS) possesses two functions: synthetic, which provides Met-tRNA for protein synthesis, and editing, which rejects inadvertently misactivated homocysteine. During editing, the side chain -SH group of homocysteine reacts with its activated carboxyl group forming a cyclic ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi960344v
更新日期：1996-06-25 00:00:00
abstract：:Serine hydroxymethyltransferase (SHMT) catalyzes the reversible cleavage of serine to form glycine and single carbon groups that are essential for many biosynthetic pathways. SHMT requires both pyridoxal phosphate (PLP) and tetrahydropteroylpolyglutamate (H4PteGlun) as cofactors, the latter as a carrier of the single ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9904151
更新日期：1999-06-29 00:00:00
abstract：:Many strains of Campylobacter jejuni display modified heptose residues in their capsular polysaccharides (CPS). The precursor heptose was previously shown to be GDP-d-glycero-α-d-manno-heptose, from which a variety of modifications of the sugar moiety have been observed. These modifications include the generation of 6...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00096
更新日期：2020-04-07 00:00:00
abstract：:Kinetics studies of dNTP analogues having pyrophosphate-mimicking β,γ-pCXYp leaving groups with variable X and Y substitution reveal striking differences in the chemical transition-state energy for DNA polymerase β that depend on all aspects of base-pairing configurations, including whether the incoming dNTP is a puri...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi500101z
更新日期：2014-03-25 00:00:00
abstract：:The incorporation of extensively fluorinated, or fluorous, analogues of hydrophobic amino acids into proteins potentially provides the opportunity to modulate the physicochemical properties of proteins in a predictable manner. On the basis of the properties of small fluorocarbon molecules, extensively fluorinated prot...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901481k
更新日期：2009-11-17 00:00:00
abstract：:A conformational transition is described for the polypeptide, gramicidin A, in which a dimer that forms a left-handed intertwined antiparallel helix is converted to a single-stranded amino terminus to amino terminus right-handed helix. The starting structure is determined here by solution NMR methods while reference i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00152a019
更新日期：1992-09-22 00:00:00
abstract：:The site-directed mutagenesis of a number of proposed active site residues of 5-enolpyruvyl shikimate-3-phosphate (EPSP) synthase is reported. Several of these mutations resulted in complete loss of enzyme activity indicating that these residues are probably involved with catalysis, notably K22R, K411R, D384A, R27A, R...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9815142
更新日期：1999-01-05 00:00:00
abstract：:Halogen bonding is a weak chemical force that has so far mostly found applications in crystal engineering. Despite its potential for use in drug discovery, as a new molecular tool in the direction of molecular recognition events, it has rarely been assessed in biopolymers. Motivated by this fact, we have developed a p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b00429
更新日期：2017-06-27 00:00:00
