Crystal structure of rabbit cytosolic serine hydroxymethyltransferase at 2.8 A resolution: mechanistic implications.

abstract：:In this study, we prepared several shortened and full-length insulin analogues with substitutions at position B26. We compared the binding affinities of the analogues for rat adipose membranes with their ability to lower the plasma glucose level in nondiabetic Wistar rats in vivo after subcutaneous administration, and...

journal_title：Biochemistry

authors： Záková L,Kazdová L,Hanclová I,Protivínská E,Sanda M,Budesínský M,Jirácek J

更新日期：2008-05-27 00:00:00

abstract：:Previously, we have demonstrated physical and functional interactions of the voltage-gated potassium channel Kv2.1 with the plasma membrane protein components of the exocytotic SNARE complex, syntaxin 1A, and the t-SNARE, syntaxin 1A/SNAP-25, complex. Importantly, the physical interaction of Kv2.1 with syntaxin was sh...

journal_title：Biochemistry

authors： Tsuk S,Lvov A,Michaelevski I,Chikvashvili D,Lotan I

更新日期：2008-08-12 00:00:00

abstract：:We describe a fluorescence assay that can be used to monitor helicase-catalyzed unwinding of duplex DNA continuously in real time. The assay is based on the observation that fluorescence resonance energy transfer (FRET) occurs between donor (fluorescein) and acceptor (hexachlorofluorescein) fluorophores that are in cl...

journal_title：Biochemistry

authors： Bjornson KP,Amaratunga M,Moore KJ,Lohman TM

更新日期：1994-11-29 00:00:00

abstract：:NAD(P)H:quinone oxidoreductase 1 (NQO1) is currently an emerging target in pancreatic cancer. In this report, we describe a series of indolequinones, based on 5-methoxy-1,2-dimethyl-3-[(4-nitrophenoxy)methyl]indole-4,7-dione (ES936), and evaluate NQO1 inhibition and growth inhibitory activity in the human pancreatic M...

journal_title：Biochemistry

authors： Reigan P,Colucci MA,Siegel D,Chilloux A,Moody CJ,Ross D

更新日期：2007-05-22 00:00:00

abstract：:Cysteinyl S-nitrosylation has emerged as an important post-translational modification affecting protein function in health and disease. Great emphasis has been placed on global, unbiased quantification of S-nitrosylated proteins because of physiologic and oxidative stimuli. However, current strategies have been hamper...

journal_title：Biochemistry

authors： Wiktorowicz JE,Stafford S,Rea H,Urvil P,Soman K,Kurosky A,Perez-Polo JR,Savidge TC

更新日期：2011-06-28 00:00:00

abstract：:Autophagy, an essential eukaryotic homeostasis pathway, allows the sequestration of unwanted, damaged, or harmful cytoplasmic components in vesicles called autophagosomes, permitting subsequent lysosomal degradation and nutrient recycling. Autophagosome nucleation is mediated by class III phosphatidylinositol-3-kinase...

journal_title：Biochemistry

authors： Mei Y,Su M,Sanishvili R,Chakravarthy S,Colbert CL,Sinha SC

更新日期：2016-08-02 00:00:00

abstract：:Plasminogen activator inhibitor-1 (PAI-1) is a serine protease inhibitor of the serpin superfamily which rapidly inactivates tissue plasminogen activator (tPA), but reacts with thrombin at a much slower rate. Based on the previous mutagenesis studies and the X-ray crystal structure of the thrombin E192Q-bovine pancrea...

journal_title：Biochemistry

authors： Rezaie AR

更新日期：1998-09-22 00:00:00

abstract：:In an effort to gain greater insight into the molecular mechanism of the electron-transfer reactions of cytochrome b(5), the bovine cytochrome b(5)-horse cytochrome c complex has been investigated by high-resolution multidimensional NMR spectroscopy using (13)C, (15)N-labeled cytochrome b(5) expressed from a synthetic...

journal_title：Biochemistry

authors： Hom K,Ma QF,Wolfe G,Zhang H,Storch EM,Daggett V,Basus VJ,Waskell L

更新日期：2000-11-21 00:00:00

abstract：:The major histocompatibility complex (HLA in humans) contains a relatively large set of gene loci in the HLA-D/DR region responsible for regulation of the immune response. The structural dissection of the protein products of these loci is a necessary accompaniment to understanding of this response. In this study, two ...

journal_title：Biochemistry

authors： Andrews DW,Bono MR,Strominger JL

更新日期：1982-12-21 00:00:00

abstract：:To study the engineering requirements for proton pumping in energy-converting enzymes such as cytochrome c oxidase, the thermodynamics and mechanisms of proton-coupled electron transfer in two designed heme proteins are elucidated. Both heme protein maquettes chosen, heme b-[H10A24]2 and heme b-[delta7-His]2, are four...

journal_title：Biochemistry

authors： Reddi AR,Reedy CJ,Mui S,Gibney BR

更新日期：2007-01-09 00:00:00

abstract：:In recent years, a number of bioorthogonal reactions have been developed, exemplified by click chemistry, that enable the efficient formation of a specific product, even within a highly complex chemical environment. While the exquisite selectivity and reliability of these transformations have led to their broad applic...

journal_title：Biochemistry

authors： Best MD

更新日期：2009-07-21 00:00:00

abstract：:Crude calf thymus DNA-dependent RNA polymerase, RNA polymerase B (ribonucleoside triphosphate: RNA nucleotidyltransferase, EC 2.7.7.6), was incubated with the tritium labeled, potent inhibitor [3H]amanin, in order to form the enzymatically inactive [3H]amanin-polymerase complex ([3H]A-P complex). Subsequent purificati...

journal_title：Biochemistry

authors： Brodner OG,Wieland T

更新日期：1976-08-10 00:00:00

abstract：:The interaction of the recombinant hemoglobin from Vitreoscilla sp. (VHb) with the bacterial membrane of Escherichia coli cells has been investigated by measuring the propensity of VHb to interact with monolayers formed by natural bacterial phosholipids. The measurements showed that the protein is capable of penetrati...

journal_title：Biochemistry

authors： Rinaldi AC,Bonamore A,Macone A,Boffi A,Bozzi A,Di Giulio A

更新日期：2006-04-04 00:00:00

abstract：:The mechanism by which terminal guanidino nitrogen substituted analogues of amiloride inhibit Na-Ca exchange in purified cardiac sarcolemmal membrane vesicles has been investigated. These inhibitors block both Nai-dependent Ca2+ uptake and Nao-dependent Ca2+ efflux. Inhibition of Na-Ca exchange monitored in K+ is nonc...

journal_title：Biochemistry

authors： Slaughter RS,Garcia ML,Cragoe EJ Jr,Reeves JP,Kaczorowski GJ

更新日期：1988-04-05 00:00:00

abstract：:The reduction of substrates catalyzed by nitrogenase utilizes an electron transfer (ET) chain comprised of three metalloclusters distributed between the two component proteins, designated as the Fe protein and the MoFe protein. The flow of electrons through these three metalloclusters involves ET from the [4Fe-4S] clu...

journal_title：Biochemistry

authors： Danyal K,Dean DR,Hoffman BM,Seefeldt LC

更新日期：2011-11-01 00:00:00

abstract：:Several fusions between the gene for human insulin-like growth factor I (IGF-I) and the genes for different IgG-binding fragments of staphylococcal protein A were assembled and compared regarding expression, secretion, and purification of the peptide hormone. After IgG affinity purification of the fusion proteins from...

journal_title：Biochemistry

authors： Moks T,Abrahmsén L,Holmgren E,Bilich M,Olsson A,Uhlén M,Pohl G,Sterky C,Hultberg H,Josephson S

更新日期：1987-08-25 00:00:00

abstract：:The mechanism by which the contractile machinery of muscle is assembled and maintained is not well-understood. Members of the cysteine-rich protein (CRP) family have been implicated in these processes. Three vertebrate CRPs (CRP1-3) that exhibit developmentally regulated muscle-specific expression have been identified...

journal_title：Biochemistry

authors： Yao X,Pérez-Alvarado GC,Louis HA,Pomiès P,Hatt C,Summers MF,Beckerle MC

更新日期：1999-05-04 00:00:00

abstract：:Carboxymethylation with 14 C-labeled iodoacetate of cysteine residues in wild-type beta-galactosidase from Escherichia coli and in a defective beta-galactosidase from deletion mutant strain M15 was investigated in order to determine accessible positions in the tetrameric wild-type form and the dimeric mutant M15 prote...

journal_title：Biochemistry

authors： Jörnvall H,Fowler AV,Zabin I

更新日期：1978-11-28 00:00:00

abstract：:CD6 is a member of the scavenger receptor cysteine rich protein superfamily (SRCRSF). This family includes many cell surface proteins whose three-dimensional structures and functions are presently not well understood. The extracellular region of CD6 includes 3 SRCR domains. The membrane proximal SRCR domain specifical...

journal_title：Biochemistry

authors： Bodian DL,Skonier JE,Bowen MA,Neubauer M,Siadak AW,Aruffo A,Bajorath J

更新日期：1997-03-04 00:00:00

abstract：:Immunoglobulins fragments are composed of globular domains linked by extended polypeptide segments. The molecular flexibility inherent in this arrangement allows for significant potential differences between structures observed in the crystalline state and those attained in solution. Small-angle neutron scattering mea...

journal_title：Biochemistry

authors： Schiffer M,Stevens FJ,Westholm FA,Kim SS,Carlson RD

更新日期：1982-06-08 00:00:00

abstract：:Properties of the interaction of bleomycin (Blm) and metallobleomycins [M = Zn, Cu(II), Fe(III), and HO(2)-Co(III)] with site-specific and nonspecific DNA oligomers, d(GGAAGCTTCC)(2) (I) and d(GGAAATTTCC)(2) (II), respectively, were investigated. With both 10-mers association constants increased in the series Blm A(2)...

journal_title：Biochemistry

authors： Li W,Zhao C,Xia C,Antholine WE,Petering DH

更新日期：2001-06-26 00:00:00

abstract：:Gamma-aminobutyric acid type A receptors (GABA(A) receptors) are ligand-gated chloride channels that play a central role in signal transmission within the mammalian central nervous system. Compounds that modulate specific GABA(A) receptor subtypes containing the delta-subunit are scarce but would be valuable research ...

journal_title：Biochemistry

authors： Lewis RW,Mabry J,Polisar JG,Eagen KP,Ganem B,Hess GP

更新日期：2010-06-15 00:00:00

abstract：:The surface electrical charge density and the deformability of nucleosomal arrays have been characterized by quantitative agarose gel electrophoresis. Monodisperse linear DNA (2.5-3.3 kbp) was reconstituted with histone octamers into either saturated (approximately 1 nucleosome/200-bp DNA) or subsaturated (< 1 nucleos...

journal_title：Biochemistry

authors： Fletcher TM,Krishnan U,Serwer P,Hansen JC

更新日期：1994-03-01 00:00:00

abstract：:Guided by the X-ray structure analysis of a crystalline EcoRV-d(GGGATATCCC) complex (Winkler, in preparation), we have begun to identify functionally important amino acid residues of EcoRV. We show here that Asn70, Asp74, Ser183, Asn185, Thr186, and Asn188 are most likely involved in the binding and/or cleavage of the...

journal_title：Biochemistry

authors： Thielking V,Selent U,Köhler E,Wolfes H,Pieper U,Geiger R,Urbanke C,Winkler FK,Pingoud A

更新日期：1991-07-02 00:00:00

abstract：:The protein tyrosine kinase, pp60(c-)(src), is involved in cellular signaling and is activated during mitosis and in various tumors. We have been employing cyclic decapeptides to identify the determinants for substrate binding and phosphorylation to develop inhibitors competitive with protein substrates of Src. A stru...

journal_title：Biochemistry

authors： Wang W,Ramdas L,Sun G,Ke S,Obeyesekere NU,Budde RJ,McMurray JS

更新日期：2000-05-02 00:00:00

abstract：:The rate of the reaction between p-nitroperoxybenzoic acid and cytochrome c peroxidase (CcP) has been investigated as a function of pH and ionic strength. The pH dependence of the reaction between CcP and peracetic acid has also been determined. The rate of the reactions are influenced by two heme-linked ionizations i...

journal_title：Biochemistry

authors： Palamakumbura AH,Foshay MC,Vitello LB,Erman JE

更新日期：1999-11-23 00:00:00

abstract：:Differential scanning calorimetry and CD spectrometry were employed to study the thermal unfolding of light meromyosin (LMM) prepared from carp acclimated to different temperatures. The transition temperatures given by the major peaks at pH 8.0 in 0.6 M KCl for LMM from carp acclimated to 10 degrees C were 32.5 and 39...

journal_title：Biochemistry

authors： Nakaya M,Kakinuma M,Watabe S,Ooi T

更新日期：1997-07-29 00:00:00

abstract：:Alpha2u-globulin is a male rat liver protein under multihormonal control which represents approximately 1% of hepatic protein synthesis. We have measured the rate of transcription of the alpha2u-globulin gene using a nuclear cell-free transcriptional system with mercurated CTP as substrate for the endogenous RNA polym...

journal_title：Biochemistry

authors： Chan KM,Kurtz DT,Feigelson P

更新日期：1978-07-25 00:00:00

abstract：:Beetle luciferases produce different bioluminescence colors from green to red using the same d-luciferin substrate. Despite many studies of the mechanisms and structural determinants of bioluminescence colors with firefly luciferases, the identity of the emitters and the specific active site interactions responsible f...

journal_title：Biochemistry

authors： Viviani VR,Neves DR,Amaral DT,Prado RA,Matsuhashi T,Hirano T

更新日期：2014-08-19 00:00:00

abstract：:N-Methylpurine-DNA glycosylase (MPG), a ubiquitous DNA repair enzyme, is responsible for the removal of a wide variety of alkylated base lesions in DNA, e.g., N-alkylpurines and cyclic ethenoadducts of adenine, guanine, and cytosine. These lesions, some of which are mutagenic and toxic, are generated endogenously or b...

journal_title：Biochemistry

authors： Roy R,Biswas T,Hazra TK,Roy G,Grabowski DT,Izumi T,Srinivasan G,Mitra S

更新日期：1998-01-13 00:00:00